MD06G1041000.v1.1

Belongs to the expansin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0018044
Physical Location & Seq
Reverse (-)
5217113 .. 5219045
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1041000.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGCTTTCTCTCTTGGGTCTCAAGTACTCTCCCTTCTCATTGTTTTGCTTTTACTGCAATCTCCGGGCGAGGCTGCAACGTGCAGTGACTGCTTCGTTCACTCTCGAGCAACTTATTATCCGAATTCTGAAGAACAAGGAACAGACGTTGGTGCATGTGGATTTGGTTCATTTGGAGCAACACTCAATGGCGGGGATGTCTCAGCTGCCTCTGACCTCTATCGCAGTGGCCTCGGATGCGGAGCTTGTTACCAGGTGAGGTGCACCGACAGTGCCTATTGCTCAGACAAAGGAGTAACTGCAGTTATAACTGACCAAGGCTCGAGTGATGGCACAGACTTCATCCTGAGTAAGCGAGCGTTTGGCCGGATGGCTCAAACTACTGAAGCAGCTGCTTCTCTGCAGTCACTTGGTGTCATTGATATCGAGTACAGACGTGTTTCATGCAGCTATCCAAACAAAAATATTACAATCAAGATCGATGAGCACAGCAACAATCCTTATTATTTGGCTTTTGTAATAGAGTACCAGCAAGGGAAGAAAGATATCACCGCTGTCCAGCTGTGTGAGACAACAAACTTTGTATGCATAGTAATGGACAGGAGTTTTGGAGCAGTTTGGACAACAACTTCAGCACCAATTGGACCCTTGCAAATAAGAATGTTATTCAGCGATGATGATGACGAGACTTGGGTTGTTCCTCTGAACACTATACCACAAGACTGGAAAGTTGGACAAACATATGACACTGGAGTTCAAGTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

256

Amino Acids

27.53

Weight (kDa)

4.45

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 71 - 144 2.5e-11 Lytic transglycolase
Expansin_C PF01357 156 - 239 6e-15 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018044)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21120
malus_domestica MD06G1041000.v1.1
prunus_persica Prupe.5G047300_v2.0.a1 Prupe.5G047300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0208241
rosa_laevigata RLG00000003236
rosa_multiflora Rmu_sc0007869.1_g000018
rosa_roxburghii Rroxscaffold_3G00250650
rosa_rugosa Rorug07G0102400
rosa_samantha Rh7CG252500
rosa_wichuraiana Rw7G020190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 308
AciI CCGC 3 cut(s) 192, 240, 552
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 3 cut(s) 150, 405, 615
AdeI CACNNNGTG 1 cut(s) 413
AfaI GTAC 3 cut(s) 27, 431, 527
AflIII ACRYGT 1 cut(s) 436
AgsI TTSAA 1 cut(s) 758
AjiI CACGTC 1 cut(s) 437
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 5 cut(s) 206, 245, 392, 450, 562
AluI AGCT 5 cut(s) 206, 245, 392, 450, 562
Alw21I GWGCWC 2 cut(s) 266, 489
Alw26I GTCTC 4 cut(s) 24, 205, 563, 680
Alw44I GTGCAC 1 cut(s) 262
AlwNI CAGNNNCTG 1 cut(s) 90
Ama87I CYCGRG 2 cut(s) 105, 322
AoxI GGCC 2 cut(s) 229, 364
ApaLI GTGCAC 1 cut(s) 262
ApeKI GCWGC 5 cut(s) 74, 206, 389, 392, 447
ApoI RAATTY 1 cut(s) 124
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
AspS9I GGNCC 1 cut(s) 644
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 268, 541
AvaI CYCGRG 2 cut(s) 105, 322
AvaII GGWCC 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 266
BarI GAAGNNNNNNTAC 2 cut(s) 18, 50
Bbv12I GWGCWC 2 cut(s) 266, 489
BbvI GCAGC 5 cut(s) 61, 193, 379, 401, 459
BccI CCATC 2 cut(s) 323, 364
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciT130I CCWGG 1 cut(s) 254
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 4 cut(s) 24, 205, 563, 680
BfmI CTRYAG 2 cut(s) 300, 401
BisI GCNGC 5 cut(s) 75, 207, 390, 393, 448
BlsI GCNGC 5 cut(s) 76, 208, 391, 394, 449
BmcAI AGTACT 1 cut(s) 27
Bme1390I CCNGG 2 cut(s) 66, 254
Bme18I GGWCC 1 cut(s) 644
BmeT110I CYCGRG 2 cut(s) 105, 322
BmgBI CACGTC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 1 cut(s) 646
BmrFI CCNGG 2 cut(s) 66, 254
BmsI GCATC 1 cut(s) 227
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpuEI CTTGAG 1 cut(s) 6
BpuMI CCSGG 1 cut(s) 66
Bsa29I ATCGAT 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 232, 316
BsaXI ACNNNNNCTCC 1 cut(s) 744
Bse1I ACTGG 2 cut(s) 728, 754
BseBI CCWGG 1 cut(s) 254
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 232, 316
BseGI GGATG 4 cut(s) 202, 242, 342, 375
BseMII CTCAG 3 cut(s) 216, 297, 338
BseNI ACTGG 2 cut(s) 728, 754
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 5 cut(s) 61, 193, 379, 401, 459
BsgI GTGCAG 1 cut(s) 103
BshFI GGCC 2 cut(s) 231, 366
BshVI ATCGAT 1 cut(s) 480
BsiHKAI GWGCWC 2 cut(s) 266, 489
BsiHKCI CYCGRG 2 cut(s) 105, 322
BsiSI CCGG 2 cut(s) 65, 367
BsmAI GTCTC 4 cut(s) 24, 205, 563, 680
BsnI GGCC 2 cut(s) 231, 366
Bso31I GGTCTC 1 cut(s) 24
BsoBI CYCGRG 2 cut(s) 105, 322
Bsp1286I GDGCHC 2 cut(s) 266, 489
Bsp143I GATC 1 cut(s) 477
BspACI CCGC 3 cut(s) 192, 240, 552
BspANI GGCC 2 cut(s) 231, 366
BspCNI CTCAG 3 cut(s) 215, 296, 339
BspDI ATCGAT 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 646
BspMAI CTGCAG 2 cut(s) 304, 405
BspTNI GGTCTC 1 cut(s) 24
BsrI ACTGG 2 cut(s) 728, 754
BssECI CCNNGG 2 cut(s) 232, 316
BssMI GATC 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 3 cut(s) 202, 283, 347
BstF5I GGATG 4 cut(s) 202, 242, 342, 375
BstKTI GATC 1 cut(s) 480
BstMAI GTCTC 4 cut(s) 24, 205, 563, 680
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 3 cut(s) 55, 90, 237
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 64, 252
BstSFI CTRYAG 2 cut(s) 300, 401
BstSLI GKGCMC 1 cut(s) 266
BstV1I GCAGC 5 cut(s) 61, 193, 379, 401, 459
BstXI CCANNNNNNTGG 1 cut(s) 723
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 2 cut(s) 231, 366
BsuTUI ATCGAT 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 687
BtrI CACGTC 1 cut(s) 437
BtsCI GGATG 4 cut(s) 202, 242, 342, 375
BtsI GCAGTG 2 cut(s) 91, 232
BtsIMutI CAGTG 4 cut(s) 91, 232, 277, 747
Cac8I GCNNGC 1 cut(s) 357
CaiI CAGNNNCTG 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 644
ClaI ATCGAT 1 cut(s) 480
CsiI ACCWGGT 1 cut(s) 252
Csp6I GTAC 3 cut(s) 26, 430, 526
CviAII CATG 2 cut(s) 156, 444
CviQI GTAC 3 cut(s) 26, 430, 526
DdeI CTNAG 3 cut(s) 202, 283, 347
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
DraIII CACNNNGTG 1 cut(s) 413
EaeI YGGCCR 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 316
Eco31I GGTCTC 1 cut(s) 24
Eco32I GATATC 2 cut(s) 424, 547
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 3 cut(s) 150, 405, 615
Eco88I CYCGRG 2 cut(s) 105, 322
EcoRI GAATTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 252
EcoRV GATATC 2 cut(s) 424, 547
EcoT14I CCWWGG 1 cut(s) 316
EcoT22I ATGCAT 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 2 cut(s) 159, 447
FaiI YATR 8 cut(s) 157, 308, 445, 586, 590, 713, 742, 744
FatI CATG 2 cut(s) 155, 443
FauI CCCGC 1 cut(s) 185
FauNDI CATATG 1 cut(s) 742
Fnu4HI GCNGC 5 cut(s) 75, 207, 390, 393, 448
FokI GGATG 4 cut(s) 209, 249, 329, 382
Fsp4HI GCNGC 5 cut(s) 75, 207, 390, 393, 448
GluI GCNGC 5 cut(s) 75, 207, 390, 393, 448
HaeIII GGCC 2 cut(s) 231, 366
HapII CCGG 2 cut(s) 65, 367
Hin1II CATG 2 cut(s) 159, 447
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HpaI GTTAAC 1 cut(s) 763
HpaII CCGG 2 cut(s) 65, 367
HphI GGTGA 2 cut(s) 268, 541
Hpy166II GTNNAC 3 cut(s) 100, 264, 763
Hpy188I TCNGA 6 cut(s) 123, 130, 214, 236, 286, 705
Hpy188III TCNNGA 3 cut(s) 105, 346, 475
Hpy8I GTNNAC 3 cut(s) 100, 264, 763
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 3 cut(s) 80, 147, 436
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 90, 237
HpyF3I CTNAG 3 cut(s) 202, 283, 347
HpySE526I ACGT 3 cut(s) 80, 147, 436
Hsp92II CATG 2 cut(s) 159, 447
KspAI GTTAAC 1 cut(s) 763
Kzo9I GATC 1 cut(s) 477
LmnI GCTCC 3 cut(s) 176, 242, 611
Lsp1109I GCAGC 5 cut(s) 61, 193, 379, 401, 459
LweI GCATC 1 cut(s) 227
MabI ACCWGGT 1 cut(s) 252
MaeII ACGT 3 cut(s) 80, 147, 436
MaeIII GTNAC 4 cut(s) 86, 248, 295, 405
MalI GATC 1 cut(s) 479
MboI GATC 1 cut(s) 477
MboII GAAGA 2 cut(s) 143, 550
MfeI CAATTG 1 cut(s) 639
MhlI GDGCHC 2 cut(s) 266, 489
MluCI AATT 2 cut(s) 124, 639
MmeI TCCRAC 1 cut(s) 712
MnlI CCTC 6 cut(s) 64, 220, 227, 242, 252, 711
Mph1103I ATGCAT 1 cut(s) 590
MseI TTAA 1 cut(s) 762
MslI CAYNNNNRTG 1 cut(s) 593
MspA1I CMGCKG 4 cut(s) 206, 392, 554, 562
MspI CCGG 2 cut(s) 65, 367
MspR9I CCNGG 2 cut(s) 66, 254
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 55, 90, 237
NciI CCSGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 742
NdeII GATC 1 cut(s) 477
NlaIII CATG 2 cut(s) 159, 447
NlaIV GGNNCC 1 cut(s) 646
NmuCI GTSAC 2 cut(s) 86, 405
NsiI ATGCAT 1 cut(s) 590
NspI RCATGY 1 cut(s) 159
PaeR7I CTCGAG 2 cut(s) 105, 322
PkrI GCNGC 5 cut(s) 76, 208, 391, 394, 449
PsiI TTATAA 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 646
PspPI GGNCC 1 cut(s) 644
PspXI VCTCGAGB 1 cut(s) 322
PstI CTGCAG 2 cut(s) 304, 405
PstNI CAGNNNCTG 1 cut(s) 90
PvuII CAGCTG 3 cut(s) 206, 392, 562
RsaI GTAC 3 cut(s) 27, 431, 527
RsaNI GTAC 3 cut(s) 26, 430, 526
RseI CAYNNNNRTG 1 cut(s) 593
SaqAI TTAA 1 cut(s) 762
SatI GCNGC 5 cut(s) 75, 207, 390, 393, 448
Sau3AI GATC 1 cut(s) 477
Sau96I GGNCC 1 cut(s) 644
ScaI AGTACT 1 cut(s) 27
ScrFI CCNGG 2 cut(s) 66, 254
SduI GDGCHC 2 cut(s) 266, 489
SexAI ACCWGGT 1 cut(s) 252
SfaNI GCATC 1 cut(s) 227
SfcI CTRYAG 2 cut(s) 300, 401
Sfr274I CTCGAG 2 cut(s) 105, 322
SinI GGWCC 1 cut(s) 644
SlaI CTCGAG 2 cut(s) 105, 322
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 3 cut(s) 21, 105, 322
SmoI CTYRAG 3 cut(s) 21, 105, 322
Sse9I AATT 2 cut(s) 124, 639
SsiI CCGC 3 cut(s) 192, 240, 552
SspI AATATT 1 cut(s) 466
StyD4I CCNGG 2 cut(s) 64, 252
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 3 cut(s) 83, 150, 439
TaqI TCGA 4 cut(s) 106, 323, 426, 480
TasI AATT 2 cut(s) 124, 639
TatI WGTACW 2 cut(s) 25, 429
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 4 cut(s) 91, 232, 277, 754
TseFI GTSAC 2 cut(s) 86, 405
TseI GCWGC 5 cut(s) 74, 206, 389, 392, 447
Tsp45I GTSAC 2 cut(s) 86, 405
TspDTI ATGAA 3 cut(s) 159, 331, 432
TspRI CASTG 4 cut(s) 91, 232, 277, 754
VneI GTGCAC 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 1 cut(s) 124
XceI RCATGY 1 cut(s) 159
XhoI CTCGAG 2 cut(s) 105, 322
ZrmI AGTACT 1 cut(s) 27
Zsp2I ATGCAT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.