Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0008932

Physical Location & Seq

Genomic Coordinates Forward (+)

12736585 .. 12742890

Gene Structure 6306 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g21220.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGGGGTCTAAGCAGAATCCTCCAGGAAACAGAACAAGACGCACATTGTCTATGTTTGTTGTGATCGCTCTGTGCTGTTTCTTCTACCTTCTTGGAGCATGGCAAAAGAGTGGTTTTGGCAAAGGAGATAAGATAGCATTCGAAGTACCCAGGCATACAGACTGCAATGTCTTTGGTAGTAATTTGAATTTTGAACCTCATCACAATGACGTGGAGATAATTGAACACTCTGAACCGAAAGTTGAGGAGTTCAAGCCTTGTGATCCTAAGTACAGGGATTATACTCCTTGCCAAGAGCAAGACCGAGCGATGAAGTTCCCAAGGGAAAATATGGTATACAGAGAAAGACATTGCCCCACAGAAGAAGAAAAATTGCACTGTCTTATTCCAGCACCGGAAGGGTATATCACTCCTTTTCCCTGGCCTAAAAGTCGCGACTATGCCCACTTTGCTAATGTCCCTTACAGACACCTGGCAGTTGAGAAAGCTGTTCAGAACTGGGTAGAGTTTAAGGGTGATGTTTTTAAATTTCCAGGTGGAGGAACAATGTTCCCTCACGGTGCCGATGCATATATTGATGAACTTGCATCAGTTATTCCCATTAAAGATGGCTCTGTGAGAACAGCATTGGATACTGGCTGTGGGGTCGCAAGCTGGGGAGCATACTTGCTGAAGAGAAATGTATTAGCTATGTCATTTGCACCGAAGGACAATCATGAAGCACAAGTTCAGTTTGCATTGGAGCGAGGTGTACCTGCAGTTATTGGTGTTCTTGGATCAATACGCCTTCCATACCCATCGAGGGCCTTTGATATGTCTCAGTGCTCTCGATGTCTGATTCCATGGACTGCAAATGATGGGATGTACCTTAAAGAAGTTGATCGGGTCCTTAGACCTGGGGGATATTGGATCTTGTCTGGACCTCCAATTAATTGGAAGACTTACTACAAAACATGGAAGAGGTCCAAGGAGGATGTCCAGGCAGAGCAAAGGAAGATTGAAGAACTAGCTGAAAGCCTGTGCTGGGTGAAAAAGTACGAGAAAGGAGATATTGCTATCTGGAAGAAGAAAGCTAATACCAATTCCTGCCAAAGGGAGTCTGCCAATATATGCCGAACAAAGGATGCTGATGATGTCTGGTACAAGAAAATGGACACATGTGTAACCCCTCCCAGTGCAAATGATGTAGCTGTAGGGCAGTTGAAGTTTCCCGCGAGGCTTTATGCAGTCCCTCCTCGAATAGCCAATGGATTAGTTGCAGGGGTGACAATTGAATCTTACCAAGAAGACAATAAGCTTTGGAAAAAGCGAGTTAATTCCTACAAAAGAATCAACAAATTAATTGGCACTACAAGATATCGGAATGTCATGGATATGAATGCTGGCCTTGGAGGATTTGCAGCAGCCCTTGAATCAAAGAACTCTTGGGTTATGAATGTCGTACCTTCAATAGCAAAGAACACTTTAGGTGTTGTTTATGAGAGAGGTCTAATTGGCATATACCACGACTGGTGTGAAGGTTTCTCTACATATCCAAGGACATATGACCTTATCCATGCTAGTGGGGTGTTCAGCTTGTACAGGAATGAGTGCAAAGTGGAAGACATCCTTTTGGAGATGGACCGCATCTTGCGGCCTGAAGGGACAGTCATCTTAAGGGACGAGGTCAATGTCCTGAACAAGGTTAAGAAGATCGCTGGGGCCATGAGATGGGATACCAAAATGATGGACCATGAGGATGGACCTCTTGTACCTGAGAAGGTACTGGTTGTTGTTAAACAGTACTGGGTTGAAAGCAGCGGAAACAGCACATCCAGTGATGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009505 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0030312 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

609

Amino Acids

69.13

Weight (kDa)

8.5

Isoelectric Point (pI)

37.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	93 - 504	6.3e-182	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	210 - 304	2.5e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18030
fragaria_vesca	FvH4_5g21220 FvH4_5g21220 FvH4_5g21220 FvH4_5g21220 FvH4_5g21220
malus_domestica	MD06G1039700.v1.1 MD16G1269600.v1.1
prunus_persica	Prupe.5G045100_v2.0.a1 Prupe.5G045100_v2.0.a1
pyrus_communis	pycom06g03330 pycom16g23860
rosa_chinensis	RchiOBHm_Chr7g0208361
rosa_laevigata	RLG00000003219
rosa_multiflora	Rmu_ssc0000063.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250520
rosa_rugosa	Rorug07G0103900
rosa_samantha	Rh7AG238300 Rh7BG233800 Rh7CG253800 Rh7DG244000
rosa_wichuraiana	Rw7G020310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	167
Acc36I	ACCTGC	1 cut(s)	763
AccB1I	GGYRCC	1 cut(s)	560
AccB7I	CCANNNNNTGG	1 cut(s)	1710
AccI	GTMKAC	1 cut(s)	336
AccII	CGCG	2 cut(s)	435, 1214
AciI	CCGC	4 cut(s)	1212, 1624, 1633, 1800
AclWI	GGATC	3 cut(s)	257, 784, 917
AcsI	RAATTY	2 cut(s)	187, 527
AcuI	CTGAAG	2 cut(s)	692, 1659
AfiI	CCNNNNNNNGG	7 cut(s)	539, 802, 1024, 1092, 1120, 1681, 1710
AflII	CTTAAG	1 cut(s)	1654
AflIII	ACRYGT	1 cut(s)	1157
AjiI	CACGTC	1 cut(s)	211
AjnI	CCWGG	7 cut(s)	22, 149, 419, 471, 532, 895, 978
AluBI	AGCT	8 cut(s)	488, 654, 689, 1010, 1073, 1190, 1297, 1575
AluI	AGCT	8 cut(s)	488, 654, 689, 1010, 1073, 1190, 1297, 1575
Alw21I	GWGCWC	1 cut(s)	827
Alw26I	GTCTC	1 cut(s)	822
AlwI	GGATC	3 cut(s)	257, 784, 917
AoxI	GGCC	5 cut(s)	422, 804, 1384, 1634, 1701
ApeKI	GCWGC	3 cut(s)	1400, 1403, 1797
ApoI	RAATTY	2 cut(s)	187, 527
AseI	ATTAAT	2 cut(s)	930, 1340
AspS9I	GGNCC	8 cut(s)	804, 886, 920, 963, 1621, 1701, 1729, 1742
AsuHPI	GGTGA	3 cut(s)	527, 1039, 1276
AsuII	TTCGAA	1 cut(s)	141
AvaII	GGWCC	6 cut(s)	886, 920, 963, 1621, 1729, 1742
BanI	GGYRCC	1 cut(s)	560
BarI	GAAGNNNNNNTAC	2 cut(s)	929, 961
BbsI	GAAGAC	3 cut(s)	944, 1293, 1608
Bbv12I	GWGCWC	1 cut(s)	827
BbvI	GCAGC	3 cut(s)	1412, 1415, 1809
BccI	CCATC	7 cut(s)	602, 805, 851, 1612, 1704, 1720, 1733
BciT130I	CCWGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
BciVI	GTATCC	2 cut(s)	625, 1708
BcoDI	GTCTC	1 cut(s)	822
BfaI	CTAG	2 cut(s)	1007, 1560
BfmI	CTRYAG	2 cut(s)	756, 1191
BfrI	CTTAAG	1 cut(s)	1654
BfuAI	ACCTGC	1 cut(s)	763
BfuI	GTATCC	2 cut(s)	625, 1708
BisI	GCNGC	4 cut(s)	1401, 1404, 1634, 1798
BlsI	GCNGC	4 cut(s)	1402, 1405, 1635, 1799
BmcAI	AGTACT	1 cut(s)	1784
Bme1390I	CCNGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
Bme18I	GGWCC	6 cut(s)	886, 920, 963, 1621, 1729, 1742
BmgBI	CACGTC	1 cut(s)	211
BmgT120I	GGNCC	8 cut(s)	804, 886, 920, 963, 1621, 1701, 1729, 1742
BmiI	GGNNCC	3 cut(s)	562, 887, 1702
BmrFI	CCNGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
BmrI	ACTGGG	3 cut(s)	508, 1167, 1795
BmsI	GCATC	4 cut(s)	556, 596, 1114, 1635
BmuI	ACTGGG	3 cut(s)	508, 1167, 1795
BpiI	GAAGAC	3 cut(s)	944, 1293, 1608
BpmI	CTGGAG	1 cut(s)	6
Bpu14I	TTCGAA	1 cut(s)	141
BsaJI	CCNNGG	8 cut(s)	149, 320, 419, 842, 896, 966, 1387, 1535
BsaWI	WCCGGW	1 cut(s)	394
Bsc4I	CCNNNNNNNGG	7 cut(s)	539, 802, 1024, 1092, 1120, 1681, 1710
Bse1I	ACTGG	7 cut(s)	503, 640, 1173, 1514, 1770, 1790, 1815
Bse3DI	GCAATG	2 cut(s)	172, 349
BseBI	CCWGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
BseDI	CCNNGG	8 cut(s)	149, 320, 419, 842, 896, 966, 1387, 1535
BseGI	GGATG	6 cut(s)	867, 979, 1129, 1605, 1744, 1811
BseLI	CCNNNNNNNGG	7 cut(s)	539, 802, 1024, 1092, 1120, 1681, 1710
BseMI	GCAATG	2 cut(s)	172, 349
BseMII	CTCAG	2 cut(s)	833, 1746
BseNI	ACTGG	7 cut(s)	503, 640, 1173, 1514, 1770, 1790, 1815
BseRI	GAGGAG	2 cut(s)	260, 1224
BseXI	GCAGC	3 cut(s)	1412, 1415, 1809
BseYI	CCCAGC	3 cut(s)	654, 1023, 1697
Bsh1236I	CGCG	2 cut(s)	435, 1214
BshFI	GGCC	5 cut(s)	424, 806, 1386, 1636, 1703
BshNI	GGYRCC	1 cut(s)	560
BsiHKAI	GWGCWC	1 cut(s)	827
BsiSI	CCGG	1 cut(s)	395
BslFI	GGGAC	4 cut(s)	443, 1214, 1657, 1673
BslI	CCNNNNNNNGG	7 cut(s)	539, 802, 1024, 1092, 1120, 1681, 1710
BsmAI	GTCTC	1 cut(s)	822
BsmFI	GGGAC	4 cut(s)	443, 1214, 1657, 1673
BsmI	GAATGC	2 cut(s)	137, 1384
BsnI	GGCC	5 cut(s)	424, 806, 1386, 1636, 1703
Bsp119I	TTCGAA	1 cut(s)	141
Bsp1286I	GDGCHC	1 cut(s)	827
Bsp1407I	TGTACA	1 cut(s)	1578
Bsp143I	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
Bsp19I	CCATGG	1 cut(s)	842
Bsp68I	TCGCGA	1 cut(s)	435
BspACI	CCGC	4 cut(s)	1212, 1624, 1633, 1800
BspANI	GGCC	5 cut(s)	424, 806, 1386, 1636, 1703
BspCNI	CTCAG	2 cut(s)	832, 1747
BspFNI	CGCG	2 cut(s)	435, 1214
BspHI	TCATGA	1 cut(s)	715
BspLI	GGNNCC	3 cut(s)	562, 887, 1702
BspMAI	CTGCAG	1 cut(s)	760
BspMI	ACCTGC	1 cut(s)	763
BspPI	GGATC	3 cut(s)	257, 784, 917
BspT104I	TTCGAA	1 cut(s)	141
BspT107I	GGYRCC	1 cut(s)	560
BspTI	CTTAAG	1 cut(s)	1654
BsrDI	GCAATG	2 cut(s)	172, 349
BsrGI	TGTACA	1 cut(s)	1578
BsrI	ACTGG	7 cut(s)	503, 640, 1173, 1514, 1770, 1790, 1815
BssECI	CCNNGG	8 cut(s)	149, 320, 419, 842, 896, 966, 1387, 1535
BssMI	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
BssNAI	GTATAC	1 cut(s)	337
BssT1I	CCWWGG	5 cut(s)	320, 842, 966, 1387, 1535
Bst1107I	GTATAC	1 cut(s)	337
Bst2UI	CCWGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
Bst4CI	ACNGT	4 cut(s)	380, 560, 1648, 1782
Bst6I	CTCTTC	2 cut(s)	668, 953
BstAFI	CTTAAG	1 cut(s)	1654
BstAUI	TGTACA	1 cut(s)	1578
BstBI	TTCGAA	1 cut(s)	141
BstC8I	GCNNGC	2 cut(s)	652, 1384
BstDEI	CTNAG	5 cut(s)	9, 267, 819, 890, 1755
BstDSI	CCRYGG	1 cut(s)	842
BstENI	CCTNNNNNAGG	2 cut(s)	1090, 1679
BstF5I	GGATG	6 cut(s)	867, 979, 1129, 1605, 1744, 1811
BstFNI	CGCG	2 cut(s)	435, 1214
BstKTI	GATC	6 cut(s)	66, 265, 779, 883, 912, 1695
BstMAI	GTCTC	1 cut(s)	822
BstMBI	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
BstMWI	GCNNNNNNNGC	2 cut(s)	449, 1806
BstNI	CCWGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
BstNSI	RCATGY	1 cut(s)	1161
BstSCI	CCNGG	7 cut(s)	22, 149, 419, 471, 532, 895, 978
BstSFI	CTRYAG	2 cut(s)	756, 1191
BstUI	CGCG	2 cut(s)	435, 1214
BstV1I	GCAGC	3 cut(s)	1412, 1415, 1809
BstV2I	GAAGAC	3 cut(s)	944, 1293, 1608
BstX2I	RGATCY	1 cut(s)	909
BstXI	CCANNNNNNTGG	4 cut(s)	933, 1562, 1726, 1739
BstYI	RGATCY	1 cut(s)	909
BstZ17I	GTATAC	1 cut(s)	337
BsuI	GTATCC	2 cut(s)	625, 1708
BsuRI	GGCC	5 cut(s)	424, 806, 1386, 1636, 1703
BtgI	CCRYGG	1 cut(s)	842
BtgZI	GCGATG	1 cut(s)	323
BtrI	CACGTC	1 cut(s)	211
BtsCI	GGATG	6 cut(s)	867, 979, 1129, 1605, 1744, 1811
BtsIMutI	CAGTG	4 cut(s)	376, 827, 1180, 1822
BtuMI	TCGCGA	1 cut(s)	435
BveI	ACCTGC	1 cut(s)	763
Cac8I	GCNNGC	2 cut(s)	652, 1384
CciI	TCATGA	1 cut(s)	715
Cfr13I	GGNCC	8 cut(s)	804, 886, 920, 963, 1621, 1701, 1729, 1742
CseI	GACGC	1 cut(s)	48
CviAII	CATG	9 cut(s)	99, 716, 843, 954, 1158, 1369, 1556, 1705, 1733
DdeI	CTNAG	5 cut(s)	9, 267, 819, 890, 1755
DpnI	GATC	6 cut(s)	65, 264, 778, 882, 911, 1694
DpnII	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
DraI	TTTAAA	1 cut(s)	526
DrdI	GACNNNNNNGTC	1 cut(s)	167
DseDI	GACNNNNNNGTC	1 cut(s)	167
Eam1104I	CTCTTC	2 cut(s)	668, 953
EarI	CTCTTC	2 cut(s)	668, 953
Eco130I	CCWWGG	5 cut(s)	320, 842, 966, 1387, 1535
Eco32I	GATATC	1 cut(s)	1358
Eco47I	GGWCC	6 cut(s)	886, 920, 963, 1621, 1729, 1742
Eco57I	CTGAAG	2 cut(s)	692, 1659
EcoNI	CCTNNNNNAGG	2 cut(s)	1090, 1679
EcoO109I	RGGNCCY	2 cut(s)	804, 886
EcoRII	CCWGG	7 cut(s)	22, 149, 419, 471, 532, 895, 978
EcoRV	GATATC	1 cut(s)	1358
EcoT14I	CCWWGG	5 cut(s)	320, 842, 966, 1387, 1535
EcoT22I	ATGCAT	1 cut(s)	571
ErhI	CCWWGG	5 cut(s)	320, 842, 966, 1387, 1535
FaeI	CATG	9 cut(s)	102, 719, 846, 957, 1161, 1372, 1559, 1708, 1736
FalI	AAGNNNNNCTT	2 cut(s)	1593, 1625
FaqI	GGGAC	4 cut(s)	443, 1214, 1657, 1673
FatI	CATG	9 cut(s)	98, 715, 842, 953, 1157, 1368, 1555, 1704, 1732
FauI	CCCGC	1 cut(s)	1219
FauNDI	CATATG	1 cut(s)	1543
FblI	GTMKAC	1 cut(s)	336
Fnu4HI	GCNGC	4 cut(s)	1401, 1404, 1634, 1798
FokI	GGATG	6 cut(s)	874, 986, 1136, 1592, 1751, 1798
Fsp4HI	GCNGC	4 cut(s)	1401, 1404, 1634, 1798
FspBI	CTAG	2 cut(s)	1007, 1560
GluI	GCNGC	4 cut(s)	1401, 1404, 1634, 1798
GsaI	CCCAGC	3 cut(s)	658, 1027, 1701
GsuI	CTGGAG	1 cut(s)	6
HaeIII	GGCC	5 cut(s)	424, 806, 1386, 1636, 1703
HapII	CCGG	1 cut(s)	395
HgaI	GACGC	1 cut(s)	48
Hin1II	CATG	9 cut(s)	102, 719, 846, 957, 1161, 1372, 1559, 1708, 1736
HindIII	AAGCTT	1 cut(s)	1295
HinfI	GANTC	6 cut(s)	16, 838, 1097, 1274, 1329, 1412
HpaII	CCGG	1 cut(s)	395
HphI	GGTGA	3 cut(s)	527, 1039, 1276
Hpy166II	GTNNAC	2 cut(s)	337, 752
Hpy188I	TCNGA	4 cut(s)	232, 495, 837, 1362
Hpy188III	TCNNGA	6 cut(s)	434, 716, 828, 918, 1060, 1675
Hpy8I	GTNNAC	2 cut(s)	337, 752
HpyAV	CCTTC	8 cut(s)	98, 392, 700, 797, 1455, 1511, 1634, 1753
HpyCH4III	ACNGT	4 cut(s)	380, 560, 1648, 1782
HpyCH4IV	ACGT	1 cut(s)	210
HpyF10VI	GCNNNNNNNGC	2 cut(s)	449, 1806
HpyF3I	CTNAG	5 cut(s)	9, 267, 819, 890, 1755
HpySE526I	ACGT	1 cut(s)	210
Hsp92II	CATG	9 cut(s)	102, 719, 846, 957, 1161, 1372, 1559, 1708, 1736
Kzo9I	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
LmnI	GCTCC	3 cut(s)	95, 659, 742
Lsp1109I	GCAGC	3 cut(s)	1412, 1415, 1809
LweI	GCATC	4 cut(s)	556, 596, 1114, 1635
MaeI	CTAG	2 cut(s)	1007, 1560
MaeII	ACGT	1 cut(s)	210
MaeIII	GTNAC	2 cut(s)	1162, 1264
MalI	GATC	6 cut(s)	65, 264, 778, 882, 911, 1694
MboI	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
MfeI	CAATTG	1 cut(s)	1269
MflI	RGATCY	1 cut(s)	909
MhlI	GDGCHC	1 cut(s)	827
MlyI	GAGTC	1 cut(s)	1106
Mph1103I	ATGCAT	1 cut(s)	571
MslI	CAYNNNNRTG	4 cut(s)	204, 1373, 1560, 1737
MspA1I	CMGCKG	1 cut(s)	1800
MspCI	CTTAAG	1 cut(s)	1654
MspI	CCGG	1 cut(s)	395
MspR9I	CCNGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
MunI	CAATTG	1 cut(s)	1269
Mva1269I	GAATGC	2 cut(s)	137, 1384
MvaI	CCWGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
MvnI	CGCG	2 cut(s)	435, 1214
MwoI	GCNNNNNNNGC	2 cut(s)	449, 1806
NcoI	CCATGG	1 cut(s)	842
NdeI	CATATG	1 cut(s)	1543
NdeII	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
NlaIII	CATG	9 cut(s)	102, 719, 846, 957, 1161, 1372, 1559, 1708, 1736
NlaIV	GGNNCC	3 cut(s)	562, 887, 1702
NmuCI	GTSAC	1 cut(s)	1264
NruI	TCGCGA	1 cut(s)	435
NsiI	ATGCAT	1 cut(s)	571
NspI	RCATGY	1 cut(s)	1161
NspV	TTCGAA	1 cut(s)	141
PagI	TCATGA	1 cut(s)	715
PciI	ACATGT	1 cut(s)	1157
PctI	GAATGC	2 cut(s)	137, 1384
PfeI	GAWTC	5 cut(s)	16, 838, 1274, 1329, 1412
PflFI	GACNNNGTC	1 cut(s)	1664
PflMI	CCANNNNNTGG	1 cut(s)	1710
PfoI	TCCNGGA	1 cut(s)	22
PkrI	GCNGC	4 cut(s)	1402, 1405, 1635, 1799
PleI	GAGTC	1 cut(s)	1105
PpsI	GAGTC	1 cut(s)	1105
PpuMI	RGGWCCY	1 cut(s)	886
PscI	ACATGT	1 cut(s)	1157
PshBI	ATTAAT	2 cut(s)	930, 1340
Psp5II	RGGWCCY	1 cut(s)	886
Psp6I	CCWGG	7 cut(s)	22, 149, 419, 471, 532, 895, 978
PspFI	CCCAGC	3 cut(s)	654, 1023, 1697
PspGI	CCWGG	7 cut(s)	22, 149, 419, 471, 532, 895, 978
PspN4I	GGNNCC	3 cut(s)	562, 887, 1702
PspPI	GGNCC	8 cut(s)	804, 886, 920, 963, 1621, 1701, 1729, 1742
PspPPI	RGGWCCY	1 cut(s)	886
PstI	CTGCAG	1 cut(s)	760
PsuI	RGATCY	1 cut(s)	909
PsyI	GACNNNGTC	1 cut(s)	1664
RruI	TCGCGA	1 cut(s)	435
RseI	CAYNNNNRTG	4 cut(s)	204, 1373, 1560, 1737
SatI	GCNGC	4 cut(s)	1401, 1404, 1634, 1798
Sau3AI	GATC	6 cut(s)	63, 262, 776, 880, 909, 1692
Sau96I	GGNCC	8 cut(s)	804, 886, 920, 963, 1621, 1701, 1729, 1742
ScaI	AGTACT	1 cut(s)	1784
SchI	GAGTC	1 cut(s)	1106
ScrFI	CCNGG	7 cut(s)	24, 151, 421, 473, 534, 897, 980
SduI	GDGCHC	1 cut(s)	827
SfaNI	GCATC	4 cut(s)	556, 596, 1114, 1635
SfcI	CTRYAG	2 cut(s)	756, 1191
SfuI	TTCGAA	1 cut(s)	141
SinI	GGWCC	6 cut(s)	886, 920, 963, 1621, 1729, 1742
SmiMI	CAYNNNNRTG	4 cut(s)	204, 1373, 1560, 1737
SmlI	CTYRAG	1 cut(s)	1654
SmoI	CTYRAG	1 cut(s)	1654
SsiI	CCGC	4 cut(s)	1212, 1624, 1633, 1800
SspMI	CTAG	2 cut(s)	1007, 1560
StyD4I	CCNGG	7 cut(s)	22, 149, 419, 471, 532, 895, 978
StyI	CCWWGG	5 cut(s)	320, 842, 966, 1387, 1535
TaaI	ACNGT	4 cut(s)	380, 560, 1648, 1782
TaiI	ACGT	1 cut(s)	213
TaqI	TCGA	4 cut(s)	141, 800, 829, 1237
TaqII	GACCGA	1 cut(s)	318
TatI	WGTACW	3 cut(s)	270, 1578, 1782
TauI	GCSGC	1 cut(s)	1636
TfiI	GAWTC	5 cut(s)	16, 838, 1274, 1329, 1412
TscAI	CASTG	4 cut(s)	383, 827, 1180, 1822
TseFI	GTSAC	1 cut(s)	1264
TseI	GCWGC	3 cut(s)	1400, 1403, 1797
Tsp45I	GTSAC	1 cut(s)	1264
TspDTI	ATGAA	5 cut(s)	326, 594, 732, 1391, 1448
TspRI	CASTG	4 cut(s)	383, 827, 1180, 1822
Tth111I	GACNNNGTC	1 cut(s)	1664
Van91I	CCANNNNNTGG	1 cut(s)	1710
Vha464I	CTTAAG	1 cut(s)	1654
VpaK11BI	GGWCC	6 cut(s)	886, 920, 963, 1621, 1729, 1742
VspI	ATTAAT	2 cut(s)	930, 1340
XagI	CCTNNNNNAGG	2 cut(s)	1090, 1679
XapI	RAATTY	2 cut(s)	187, 527
XceI	RCATGY	1 cut(s)	1161
XmiI	GTMKAC	1 cut(s)	336
XspI	CTAG	2 cut(s)	1007, 1560
ZrmI	AGTACT	1 cut(s)	1784
Zsp2I	ATGCAT	1 cut(s)	571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.