Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0008932

Physical Location & Seq

Genomic Coordinates Reverse (-)

45816148 .. 45822995

Gene Structure 6848 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003219

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGGTGGTGTGGTTCCGAAGTTTGCCCGAGGTTGGTAGATGCTTCCATCTTTACCGATATATACTTACCAGAGTTGCTAGGGAGCTTCAAATACCATTAGACCCACTTGGGCTCTCATCTCTCTCAAACCCATCAAAGAGCTCTCTAACCCATCTCAGGTTTGAGGGAATTTTTTCAACAATATTATGGGGTCTAAGCAGAATCCTCCAGGAAACAGAACAAGACGCACATTATAAGATAGCATTCCAAGTACCCAAGCAGACAGAGTGCAATGTCTTTAGTACTAATTTGAACTTTGAAACTCATCACAATGACGTGGAGATAATCGAACGCTCTGAACCAAAAGCCAAGGAGTTCAAGCCTTGTGATCCTCAGTACAGGGATTATACTCCTTGCCAAGAGCAAGACCGAGCAATGAAGTTCCCAAGGGAAAATATGGTATACAGGGAAAGACATTGTCCCCCAGAAGAGGAAAAATTGCACTGTCTTATTCCAGCACCCAAAGGGTATATTGCTCCGTTTCCCTGGCCTAAAAGCCGCGACTATGCTCACTATGCTAATGTCCCTTATAAACACCTGGCGGTTGAGAAAGCTGTTCAGAACTGGGTAGAGTTTCAGGGTGATGTTTTTAAATTCCCAGGTGGAGGAACAATGTTCCCTCACGGTGCTGATGCATATATTGAGGAACTTGCATCAGTTATTCCAATTGCAGATGGCTCTGTGAGAACAGCATTGGATACTGGCTGTGGGGTTGCAAGCTGGGGTGCATACTTGCTGAAGAGAAATATATTAGCCATGTCATTTGCACCAAAGGACAATCATGAAGCACAAGTTCAGTTTGCATTGGAGCGAGGTGTGCCTGCAGTTATTGGTGTTCTTGGATCAATACGTCTTCCATACCCATCTAGGGCCTTTGATATGTCTCAGTGCTCTCGATGTCTGATTCCATGGGCTGCAAATGATGGGATGTACCTAAAAGAAGTTGATCGGGTCCTTAGACCTGGGGGATATTGGATCTTGTCTGGACCTCCAATTAATTGGAAGACTTACTACAAAACGTGGAAGAGGTCTAAGGAGGATGTCCAGGCAGAGCAAAGGAAGATTGAAGACCTAGCTGAAAGTCTGTGTTGGGAGAAAAAGTACGAGAAAGGAGATATTGCCATCTGGAAGAAGAAAGCTAATACGAAATCCTGCAAAAGAGAGTCTGTCAATATATGCCGAACAAAGGATGCTGATGATGTCTGGTACAAGAAAATGGACACATGTGTAACCCCTCCCAGTGCAAATGAAGTACCTGTGGGGCAGTTGAAGTTTCCTGCAAGGCTTTACGCAGTCCCTCCTCGAATAGCTAATGGATTAGTTGCAGGGGTGACAACTGAATCTTATCAAGAAGACAATAAACTTTGGAAAAAGCAAGTAAATTCGTACAAAAGAATCAACAAATTAATTGGCACTACAAGATATAGGAATGTGATGGATATGAATGCTGGCCTGGGTGGATTTGCAGCGGCCCTTGAATCACGGAAGTCTTGGGTTATGAATGTCGTACCCTCAATAGCAAAGAACACTTTAGGTGTTGTTTATGAGAGAGGTCTAATCGGCATATACCATGACTGGTGTGAAGGCTTCTCTACATATCCAAGGACATATGACCTTATTCATGCCAGTGGGGTGTTCAGCTTGTACAGGAATGAGTGCGAAGCGGAAGACATCCTGTTGGAGATGGATCGCATCTTGCGGCCTGAAGGGACAGTCATCTTAAGGGATGAGGTCGATGTCCTGAACAAGGTTCAGAAGATTGCTGGGGGTATGAGATGGGATACCAAAATGATGGACCATGAGGATGGACCCCTTGTACCTGAGAAGATACTGGTTGTTGTTAAACAGTACTGGGTTGAAAACAGCGGAAACAGCACATCTAGTGATGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009505 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0030312 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

644

Amino Acids

73.45

Weight (kDa)

8.03

Isoelectric Point (pI)

46.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	128 - 628	1.1e-227	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	245 - 339	5.9e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18030
fragaria_vesca	FvH4_5g21220 FvH4_5g21220 FvH4_5g21220 FvH4_5g21220 FvH4_5g21220
malus_domestica	MD06G1039700.v1.1 MD16G1269600.v1.1
prunus_persica	Prupe.5G045100_v2.0.a1 Prupe.5G045100_v2.0.a1
pyrus_communis	pycom06g03330 pycom16g23860
rosa_chinensis	RchiOBHm_Chr7g0208361
rosa_laevigata	RLG00000003219
rosa_multiflora	Rmu_ssc0000063.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250520
rosa_rugosa	Rorug07G0103900
rosa_samantha	Rh7AG238300 Rh7BG233800 Rh7CG253800 Rh7DG244000
rosa_wichuraiana	Rw7G020310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	234, 570
AccI	GTMKAC	1 cut(s)	441
AccII	CGCG	1 cut(s)	540
AciI	CCGC	6 cut(s)	538, 581, 1508, 1703, 1738, 1905
AclWI	GGATC	4 cut(s)	362, 889, 1022, 1734
AcsI	RAATTY	3 cut(s)	168, 632, 1420
AcuI	CTGAAG	2 cut(s)	797, 1764
AfiI	CCNNNNNNNGG	7 cut(s)	32, 156, 469, 644, 907, 1225, 1786
AflII	CTTAAG	1 cut(s)	1759
AflIII	ACRYGT	1 cut(s)	1262
AgsI	TTSAA	9 cut(s)	89, 177, 292, 299, 358, 1106, 1309, 1517, 1898
AjiI	CACGTC	1 cut(s)	316
AjnI	CCWGG	7 cut(s)	207, 524, 576, 637, 1000, 1083, 1491
AluBI	AGCT	8 cut(s)	85, 141, 593, 759, 1115, 1178, 1349, 1680
AluI	AGCT	8 cut(s)	85, 141, 593, 759, 1115, 1178, 1349, 1680
Alw21I	GWGCWC	2 cut(s)	143, 932
Alw26I	GTCTC	1 cut(s)	927
AlwI	GGATC	4 cut(s)	362, 889, 1022, 1734
Ama87I	CYCGRG	1 cut(s)	26
AoxI	GGCC	5 cut(s)	527, 909, 1489, 1509, 1739
ApeKI	GCWGC	2 cut(s)	953, 1505
ApoI	RAATTY	3 cut(s)	168, 632, 1420
AseI	ATTAAT	2 cut(s)	1035, 1445
Asp700I	GAANNNNTTC	1 cut(s)	172
AspS9I	GGNCC	6 cut(s)	909, 991, 1025, 1510, 1834, 1847
AsuHPI	GGTGA	2 cut(s)	632, 1381
AvaI	CYCGRG	1 cut(s)	26
AvaII	GGWCC	4 cut(s)	991, 1025, 1834, 1847
BanII	GRGCYC	2 cut(s)	114, 143
BarI	GAAGNNNNNNTAC	2 cut(s)	1034, 1066
BbsI	GAAGAC	5 cut(s)	884, 1049, 1113, 1398, 1713
Bbv12I	GWGCWC	2 cut(s)	143, 932
BbvI	GCAGC	2 cut(s)	940, 1517
BciT130I	CCWGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
BciVI	GTATCC	2 cut(s)	730, 1813
BcoDI	GTCTC	1 cut(s)	927
BfaI	CTAG	4 cut(s)	78, 906, 1112, 1920
BfmI	CTRYAG	1 cut(s)	861
BfrI	CTTAAG	1 cut(s)	1759
BfuI	GTATCC	2 cut(s)	730, 1813
BisI	GCNGC	5 cut(s)	538, 954, 1506, 1509, 1739
BlsI	GCNGC	5 cut(s)	539, 955, 1507, 1510, 1740
BmcAI	AGTACT	2 cut(s)	283, 1889
Bme1390I	CCNGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
Bme18I	GGWCC	4 cut(s)	991, 1025, 1834, 1847
BmeT110I	CYCGRG	1 cut(s)	26
BmgBI	CACGTC	1 cut(s)	316
BmgT120I	GGNCC	6 cut(s)	909, 991, 1025, 1510, 1834, 1847
BmiI	GGNNCC	3 cut(s)	14, 992, 1849
BmrFI	CCNGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
BmrI	ACTGGG	3 cut(s)	613, 1272, 1900
BmsI	GCATC	5 cut(s)	29, 661, 701, 1219, 1740
BmuI	ACTGGG	3 cut(s)	613, 1272, 1900
BpiI	GAAGAC	5 cut(s)	884, 1049, 1113, 1398, 1713
BpmI	CTGGAG	1 cut(s)	191
BsaJI	CCNNGG	9 cut(s)	27, 348, 425, 524, 637, 947, 1001, 1492, 1640
BsaXI	ACNNNNNCTCC	2 cut(s)	1124, 1154
Bsc4I	CCNNNNNNNGG	7 cut(s)	32, 156, 469, 644, 907, 1225, 1786
Bse1I	ACTGG	7 cut(s)	608, 745, 1278, 1619, 1665, 1875, 1895
Bse3DI	GCAATG	2 cut(s)	277, 420
BseBI	CCWGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
BseDI	CCNNGG	9 cut(s)	27, 348, 425, 524, 637, 947, 1001, 1492, 1640
BseGI	GGATG	6 cut(s)	972, 1084, 1234, 1710, 1771, 1849
BseLI	CCNNNNNNNGG	7 cut(s)	32, 156, 469, 644, 907, 1225, 1786
BseMI	GCAATG	2 cut(s)	277, 420
BseMII	CTCAG	4 cut(s)	169, 386, 938, 1851
BseNI	ACTGG	7 cut(s)	608, 745, 1278, 1619, 1665, 1875, 1895
BseRI	GAGGAG	1 cut(s)	1329
BseXI	GCAGC	2 cut(s)	940, 1517
BseYI	CCCAGC	2 cut(s)	759, 1802
Bsh1236I	CGCG	1 cut(s)	540
BshFI	GGCC	5 cut(s)	529, 911, 1491, 1511, 1741
BsiHKAI	GWGCWC	2 cut(s)	143, 932
BsiHKCI	CYCGRG	1 cut(s)	26
BslFI	GGGAC	4 cut(s)	444, 548, 1319, 1762
BslI	CCNNNNNNNGG	7 cut(s)	32, 156, 469, 644, 907, 1225, 1786
BsmAI	GTCTC	1 cut(s)	927
BsmFI	GGGAC	4 cut(s)	444, 548, 1319, 1762
BsmI	GAATGC	2 cut(s)	242, 1489
BsnI	GGCC	5 cut(s)	529, 911, 1491, 1511, 1741
BsoBI	CYCGRG	1 cut(s)	26
Bsp1286I	GDGCHC	3 cut(s)	114, 143, 932
Bsp1407I	TGTACA	1 cut(s)	1683
Bsp143I	GATC	5 cut(s)	367, 881, 985, 1014, 1726
Bsp19I	CCATGG	1 cut(s)	947
BspACI	CCGC	6 cut(s)	538, 581, 1508, 1703, 1738, 1905
BspANI	GGCC	5 cut(s)	529, 911, 1491, 1511, 1741
BspCNI	CTCAG	4 cut(s)	168, 385, 937, 1852
BspFNI	CGCG	1 cut(s)	540
BspHI	TCATGA	1 cut(s)	820
BspLI	GGNNCC	3 cut(s)	14, 992, 1849
BspMAI	CTGCAG	1 cut(s)	865
BspPI	GGATC	4 cut(s)	362, 889, 1022, 1734
BspTI	CTTAAG	1 cut(s)	1759
BsrDI	GCAATG	2 cut(s)	277, 420
BsrGI	TGTACA	1 cut(s)	1683
BsrI	ACTGG	7 cut(s)	608, 745, 1278, 1619, 1665, 1875, 1895
BssECI	CCNNGG	9 cut(s)	27, 348, 425, 524, 637, 947, 1001, 1492, 1640
BssMI	GATC	5 cut(s)	367, 881, 985, 1014, 1726
BssNAI	GTATAC	1 cut(s)	442
BssT1I	CCWWGG	4 cut(s)	348, 425, 947, 1640
Bst1107I	GTATAC	1 cut(s)	442
Bst2UI	CCWGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
Bst4CI	ACNGT	4 cut(s)	485, 665, 1753, 1887
Bst6I	CTCTTC	3 cut(s)	462, 773, 1058
BstAFI	CTTAAG	1 cut(s)	1759
BstAUI	TGTACA	1 cut(s)	1683
BstC8I	GCNNGC	3 cut(s)	757, 861, 1489
BstDEI	CTNAG	7 cut(s)	155, 194, 372, 924, 995, 1071, 1860
BstDSI	CCRYGG	1 cut(s)	947
BstENI	CCTNNNNNAGG	1 cut(s)	1784
BstF5I	GGATG	6 cut(s)	972, 1084, 1234, 1710, 1771, 1849
BstFNI	CGCG	1 cut(s)	540
BstKTI	GATC	5 cut(s)	370, 884, 988, 1017, 1729
BstMAI	GTCTC	1 cut(s)	927
BstMBI	GATC	5 cut(s)	367, 881, 985, 1014, 1726
BstMWI	GCNNNNNNNGC	3 cut(s)	554, 856, 1911
BstNI	CCWGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
BstNSI	RCATGY	1 cut(s)	1266
BstSCI	CCNGG	7 cut(s)	207, 524, 576, 637, 1000, 1083, 1491
BstSFI	CTRYAG	1 cut(s)	861
BstUI	CGCG	1 cut(s)	540
BstV1I	GCAGC	2 cut(s)	940, 1517
BstV2I	GAAGAC	5 cut(s)	884, 1049, 1113, 1398, 1713
BstX2I	RGATCY	1 cut(s)	1014
BstXI	CCANNNNNNTGG	3 cut(s)	1038, 1831, 1844
BstYI	RGATCY	1 cut(s)	1014
BstZ17I	GTATAC	1 cut(s)	442
BsuI	GTATCC	2 cut(s)	730, 1813
BsuRI	GGCC	5 cut(s)	529, 911, 1491, 1511, 1741
BtgI	CCRYGG	1 cut(s)	947
BtrI	CACGTC	1 cut(s)	316
BtsCI	GGATG	6 cut(s)	972, 1084, 1234, 1710, 1771, 1849
BtsIMutI	CAGTG	4 cut(s)	481, 932, 1285, 1672
Cac8I	GCNNGC	3 cut(s)	757, 861, 1489
CciI	TCATGA	1 cut(s)	820
Cfr13I	GGNCC	6 cut(s)	909, 991, 1025, 1510, 1834, 1847
CseI	GACGC	1 cut(s)	233
CviAII	CATG	7 cut(s)	796, 821, 948, 1263, 1610, 1661, 1838
DdeI	CTNAG	7 cut(s)	155, 194, 372, 924, 995, 1071, 1860
DpnI	GATC	5 cut(s)	369, 883, 987, 1016, 1728
DpnII	GATC	5 cut(s)	367, 881, 985, 1014, 1726
DraI	TTTAAA	1 cut(s)	631
Eam1104I	CTCTTC	3 cut(s)	462, 773, 1058
EarI	CTCTTC	3 cut(s)	462, 773, 1058
Ecl136II	GAGCTC	1 cut(s)	141
Eco130I	CCWWGG	4 cut(s)	348, 425, 947, 1640
Eco24I	GRGCYC	2 cut(s)	114, 143
Eco47I	GGWCC	4 cut(s)	991, 1025, 1834, 1847
Eco53kI	GAGCTC	1 cut(s)	141
Eco57I	CTGAAG	2 cut(s)	797, 1764
Eco88I	CYCGRG	1 cut(s)	26
EcoICRI	GAGCTC	1 cut(s)	141
EcoNI	CCTNNNNNAGG	1 cut(s)	1784
EcoO109I	RGGNCCY	2 cut(s)	909, 991
EcoRII	CCWGG	7 cut(s)	207, 524, 576, 637, 1000, 1083, 1491
EcoT14I	CCWWGG	4 cut(s)	348, 425, 947, 1640
EcoT22I	ATGCAT	1 cut(s)	676
EcoT38I	GRGCYC	2 cut(s)	114, 143
ErhI	CCWWGG	4 cut(s)	348, 425, 947, 1640
FaeI	CATG	7 cut(s)	799, 824, 951, 1266, 1613, 1664, 1841
FaqI	GGGAC	4 cut(s)	444, 548, 1319, 1762
FatI	CATG	7 cut(s)	795, 820, 947, 1262, 1609, 1660, 1837
FauNDI	CATATG	1 cut(s)	1648
FblI	GTMKAC	1 cut(s)	441
Fnu4HI	GCNGC	5 cut(s)	538, 954, 1506, 1509, 1739
FokI	GGATG	6 cut(s)	979, 1091, 1241, 1697, 1778, 1856
FriOI	GRGCYC	2 cut(s)	114, 143
Fsp4HI	GCNGC	5 cut(s)	538, 954, 1506, 1509, 1739
FspBI	CTAG	4 cut(s)	78, 906, 1112, 1920
GluI	GCNGC	5 cut(s)	538, 954, 1506, 1509, 1739
GsaI	CCCAGC	2 cut(s)	763, 1806
GsuI	CTGGAG	1 cut(s)	191
HaeIII	GGCC	5 cut(s)	529, 911, 1491, 1511, 1741
HgaI	GACGC	1 cut(s)	233
Hin1II	CATG	7 cut(s)	799, 824, 951, 1266, 1613, 1664, 1841
HinfI	GANTC	6 cut(s)	201, 943, 1202, 1379, 1434, 1517
HphI	GGTGA	2 cut(s)	632, 1381
Hpy166II	GTNNAC	1 cut(s)	442
Hpy188I	TCNGA	5 cut(s)	17, 337, 600, 942, 1794
Hpy188III	TCNNGA	6 cut(s)	821, 933, 1023, 1165, 1388, 1780
Hpy8I	GTNNAC	1 cut(s)	442
HpyAV	CCTTC	2 cut(s)	1616, 1739
HpyCH4III	ACNGT	4 cut(s)	485, 665, 1753, 1887
HpyCH4IV	ACGT	3 cut(s)	315, 889, 1058
HpyF10VI	GCNNNNNNNGC	3 cut(s)	554, 856, 1911
HpyF3I	CTNAG	7 cut(s)	155, 194, 372, 924, 995, 1071, 1860
HpySE526I	ACGT	3 cut(s)	315, 889, 1058
Hsp92II	CATG	7 cut(s)	799, 824, 951, 1266, 1613, 1664, 1841
Kzo9I	GATC	5 cut(s)	367, 881, 985, 1014, 1726
LmnI	GCTCC	3 cut(s)	82, 520, 847
Lsp1109I	GCAGC	2 cut(s)	940, 1517
LweI	GCATC	5 cut(s)	29, 661, 701, 1219, 1740
MaeI	CTAG	4 cut(s)	78, 906, 1112, 1920
MaeII	ACGT	3 cut(s)	315, 889, 1058
MaeIII	GTNAC	2 cut(s)	1267, 1369
MalI	GATC	5 cut(s)	369, 883, 987, 1016, 1728
MboI	GATC	5 cut(s)	367, 881, 985, 1014, 1726
MfeI	CAATTG	1 cut(s)	705
MflI	RGATCY	1 cut(s)	1014
MhlI	GDGCHC	3 cut(s)	114, 143, 932
MlyI	GAGTC	1 cut(s)	1211
MmeI	TCCRAC	1 cut(s)	1698
Mph1103I	ATGCAT	1 cut(s)	676
MroXI	GAANNNNTTC	1 cut(s)	172
MseI	TTAA	5 cut(s)	630, 1035, 1445, 1760, 1881
MslI	CAYNNNNRTG	3 cut(s)	309, 1665, 1842
MspA1I	CMGCKG	2 cut(s)	1508, 1905
MspCI	CTTAAG	1 cut(s)	1759
MspR9I	CCNGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
MunI	CAATTG	1 cut(s)	705
Mva1269I	GAATGC	2 cut(s)	242, 1489
MvaI	CCWGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
MvnI	CGCG	1 cut(s)	540
MwoI	GCNNNNNNNGC	3 cut(s)	554, 856, 1911
NcoI	CCATGG	1 cut(s)	947
NdeI	CATATG	1 cut(s)	1648
NdeII	GATC	5 cut(s)	367, 881, 985, 1014, 1726
NlaIII	CATG	7 cut(s)	799, 824, 951, 1266, 1613, 1664, 1841
NlaIV	GGNNCC	3 cut(s)	14, 992, 1849
NmuCI	GTSAC	1 cut(s)	1369
NsiI	ATGCAT	1 cut(s)	676
NspI	RCATGY	1 cut(s)	1266
PagI	TCATGA	1 cut(s)	820
PciI	ACATGT	1 cut(s)	1262
PctI	GAATGC	2 cut(s)	242, 1489
PdmI	GAANNNNTTC	1 cut(s)	172
PfeI	GAWTC	5 cut(s)	201, 943, 1379, 1434, 1517
PflFI	GACNNNGTC	1 cut(s)	456
PfoI	TCCNGGA	1 cut(s)	207
PkrI	GCNGC	5 cut(s)	539, 955, 1507, 1510, 1740
PleI	GAGTC	1 cut(s)	1210
PpsI	GAGTC	1 cut(s)	1210
PpuMI	RGGWCCY	1 cut(s)	991
PscI	ACATGT	1 cut(s)	1262
PshBI	ATTAAT	2 cut(s)	1035, 1445
PsiI	TTATAA	2 cut(s)	234, 570
Psp124BI	GAGCTC	1 cut(s)	143
Psp5II	RGGWCCY	1 cut(s)	991
Psp6I	CCWGG	7 cut(s)	207, 524, 576, 637, 1000, 1083, 1491
PspFI	CCCAGC	2 cut(s)	759, 1802
PspGI	CCWGG	7 cut(s)	207, 524, 576, 637, 1000, 1083, 1491
PspN4I	GGNNCC	3 cut(s)	14, 992, 1849
PspPI	GGNCC	6 cut(s)	909, 991, 1025, 1510, 1834, 1847
PspPPI	RGGWCCY	1 cut(s)	991
PstI	CTGCAG	1 cut(s)	865
PsuI	RGATCY	1 cut(s)	1014
PsyI	GACNNNGTC	1 cut(s)	456
RseI	CAYNNNNRTG	3 cut(s)	309, 1665, 1842
SacI	GAGCTC	1 cut(s)	143
SaqAI	TTAA	5 cut(s)	630, 1035, 1445, 1760, 1881
SatI	GCNGC	5 cut(s)	538, 954, 1506, 1509, 1739
Sau3AI	GATC	5 cut(s)	367, 881, 985, 1014, 1726
Sau96I	GGNCC	6 cut(s)	909, 991, 1025, 1510, 1834, 1847
ScaI	AGTACT	2 cut(s)	283, 1889
SchI	GAGTC	1 cut(s)	1211
ScrFI	CCNGG	7 cut(s)	209, 526, 578, 639, 1002, 1085, 1493
SduI	GDGCHC	3 cut(s)	114, 143, 932
SfaNI	GCATC	5 cut(s)	29, 661, 701, 1219, 1740
SfcI	CTRYAG	1 cut(s)	861
SinI	GGWCC	4 cut(s)	991, 1025, 1834, 1847
SmiMI	CAYNNNNRTG	3 cut(s)	309, 1665, 1842
SmlI	CTYRAG	1 cut(s)	1759
SmoI	CTYRAG	1 cut(s)	1759
SsiI	CCGC	6 cut(s)	538, 581, 1508, 1703, 1738, 1905
SspI	AATATT	1 cut(s)	183
SspMI	CTAG	4 cut(s)	78, 906, 1112, 1920
SstI	GAGCTC	1 cut(s)	143
StyD4I	CCNGG	7 cut(s)	207, 524, 576, 637, 1000, 1083, 1491
StyI	CCWWGG	4 cut(s)	348, 425, 947, 1640
TaaI	ACNGT	4 cut(s)	485, 665, 1753, 1887
TaiI	ACGT	3 cut(s)	318, 892, 1061
TaqI	TCGA	4 cut(s)	327, 934, 1342, 1773
TaqII	GACCGA	1 cut(s)	423
TatI	WGTACW	4 cut(s)	281, 375, 1683, 1887
TauI	GCSGC	3 cut(s)	540, 1511, 1741
TfiI	GAWTC	5 cut(s)	201, 943, 1379, 1434, 1517
Tru1I	TTAA	5 cut(s)	630, 1035, 1445, 1760, 1881
Tru9I	TTAA	5 cut(s)	630, 1035, 1445, 1760, 1881
TscAI	CASTG	4 cut(s)	488, 932, 1285, 1672
TseFI	GTSAC	1 cut(s)	1369
TseI	GCWGC	2 cut(s)	953, 1505
Tsp45I	GTSAC	1 cut(s)	1369
TspDTI	ATGAA	6 cut(s)	431, 837, 1302, 1496, 1553, 1649
TspGWI	ACGGA	2 cut(s)	507, 1537
TspRI	CASTG	4 cut(s)	488, 932, 1285, 1672
Tth111I	GACNNNGTC	1 cut(s)	456
Vha464I	CTTAAG	1 cut(s)	1759
VpaK11BI	GGWCC	4 cut(s)	991, 1025, 1834, 1847
VspI	ATTAAT	2 cut(s)	1035, 1445
XagI	CCTNNNNNAGG	1 cut(s)	1784
XapI	RAATTY	3 cut(s)	168, 632, 1420
XceI	RCATGY	1 cut(s)	1266
XmiI	GTMKAC	1 cut(s)	441
XmnI	GAANNNNTTC	1 cut(s)	172
XspI	CTAG	4 cut(s)	78, 906, 1112, 1920
ZrmI	AGTACT	2 cut(s)	283, 1889
Zsp2I	ATGCAT	1 cut(s)	676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.