Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008932

Physical Location & Seq

Genomic Coordinates Forward (+)

34827898 .. 34832764

Gene Structure 4867 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1269600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGGCCCCAAGCATAATTCTTCAGGAAACAGAACAAGAAGCACATTATCTATATTTGTTGTGATTTCTCTTTGTTGTTTCTTCTACCTTCTTGGAGCATGGCAAAAGAGCGGTTTTGGAAAAGGTGATAAGATAGCATTTCAAGTTACCAAACAGAAAGATTGCAATGTCTTTGCTGAGTTGAACTCTGATTTGAACTTTGAAACTCATCACAATGTCGTGGAGATAGTTGAACCTTCTGAACCTAAAGCCAAAGAGTTCAAACCTTGTCATGTGAAATATGCTGATTATACCCCTTGTCAAGAGCAAGACCGAGCAATGAAATTCCCCAGGGAAAATATGATATACAGGGAAAGACATTGTCCACCAGAAGAGGAAAAATTGCATTGTCTTATTCCAGCACGGGAAGGGTATATGACTCCATTCCCCTGGCCTAAAGGCCGTGACTATGTCCACTATGGAAATGTACCTTATAAAAGCCTCACAGTTGAGAAAGCTGTACAGAACTGGGTGCAATTTCAGGGAAATGTTTTCAAATTTCCAGGTGGAGGAACAATGTTCCCCCAAGGCGCAGATGCATATATTGATGAACTTGCATCAGTTATTCCGATTGCAGATGGCTCTGTCAGGACAGCATTGGATACCGGTTGTGGAGTTGCAAGTTGGGGTGCATACTTATTGAAGAGAAATGTATTAGCCATGTCCTTTGCACCGCGGGACAATCATGAAGCCCAAGTGCAGTTTGCATTGGAGCGAGGAGTACCTGCTGTTATCGGTGTGCTTGGATCGATTCGTCTTCCATACCCAGCTAGGGCCTTTGATATGGCCCAGTGCTCTCGGTGTTTAATTCCATGGACTGCAAATGATGGAATGTACCTAAAAGAAGTTGATAGGGTCCTTAGACCTGGTGGATACTGGATATTGTCTGGACCTCCAATTAATTGGAAGACCTACTACAAAACGTGGAAGCGGTCTAAGGAGGACGTGCAGGCAGAGCAAAAACAGATTGAAGCACTAGCTGAAAGTCTTTGCTGGGAGAAAAAGTATGAGAAAGGAGATATGGCTATCTGGAAGAAAAAAGTTAATACAAAATCCTGCAGGAGAAAGTCTGTCGATGTATGCCAGACAGAGGATACTGATGATGTCTGGTACAAGAAAATGGACACATGTGTAACCCCTTCTCCTGACGTAACCAATGCAAATGAAGTAGCTGTAGGGGAGTTGAAGTTTCCTGCAAGGCTTTATGCAGTCCCTCCTCGAATAGCTAATGGTTTAGTTGTGGGGGTTACAACCAAATCTTACCAAGAGGACAATAAACTTTGGAAAAAGCATGTAAATGTTTACAAAAGAATCAACAGATTGATTGGCACTACAAGATATCGGAATGTGATGGATATGAATGCATGCCTTGGAGGATTTGCAGCAGCCCTTGAATCACGAAAATTGTGGGTTATGAATGTTGTGCCTACAATTGCAAAGAATACTTTAGGTGTTATTTATGAGAGAGGTCTAATCGGCATATATCATGACTGGTGTGAAGGCTTTTCTACGTACCCAAGGTCATATGACCTTATTCATGCTAGCGGTGTGTTCAGCTTGTACAAGAACGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009505 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0030312 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

538

Amino Acids

61.01

Weight (kDa)

8.9

Isoelectric Point (pI)

34.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	96 - 536	6.4e-198	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	213 - 307	1.6e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18030
fragaria_vesca	FvH4_5g21220 FvH4_5g21220 FvH4_5g21220 FvH4_5g21220 FvH4_5g21220
malus_domestica	MD06G1039700.v1.1 MD16G1269600.v1.1
prunus_persica	Prupe.5G045100_v2.0.a1 Prupe.5G045100_v2.0.a1
pyrus_communis	pycom06g03330 pycom16g23860
rosa_chinensis	RchiOBHm_Chr7g0208361
rosa_laevigata	RLG00000003219
rosa_multiflora	Rmu_ssc0000063.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250520
rosa_rugosa	Rorug07G0103900
rosa_samantha	Rh7AG238300 Rh7BG233800 Rh7CG253800 Rh7DG244000
rosa_wichuraiana	Rw7G020310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	474
Acc36I	ACCTGC	1 cut(s)	772
AccBSI	CCGCTC	1 cut(s)	111
AccII	CGCG	1 cut(s)	715
AciI	CCGC	5 cut(s)	111, 713, 715, 970, 1584
AclWI	GGATC	1 cut(s)	793
AcsI	RAATTY	2 cut(s)	323, 536
AcuI	CTGAAG	1 cut(s)	6
AfaI	GTAC	7 cut(s)	468, 501, 762, 875, 1151, 1553, 1601
AfiI	CCNNNNNNNGG	3 cut(s)	548, 811, 1129
AflIII	ACRYGT	1 cut(s)	1166
AgeI	ACCGGT	1 cut(s)	644
AjiI	CACGTC	1 cut(s)	985
AjnI	CCWGG	4 cut(s)	329, 428, 541, 904
AluBI	AGCT	6 cut(s)	497, 809, 1019, 1211, 1265, 1596
AluI	AGCT	6 cut(s)	497, 809, 1019, 1211, 1265, 1596
Alw21I	GWGCWC	1 cut(s)	836
AlwI	GGATC	1 cut(s)	793
AoxI	GGCC	5 cut(s)	4, 431, 439, 813, 825
ApaI	GGGCCC	1 cut(s)	8
ApeKI	GCWGC	2 cut(s)	1421, 1424
ApoI	RAATTY	2 cut(s)	323, 536
AseI	ATTAAT	1 cut(s)	939
AsiGI	ACCGGT	1 cut(s)	644
AspLEI	GCGC	1 cut(s)	572
AspS9I	GGNCC	6 cut(s)	4, 5, 813, 826, 895, 929
AsuHPI	GGTGA	1 cut(s)	137
AsuNHI	GCTAGC	1 cut(s)	1580
AvaII	GGWCC	2 cut(s)	895, 929
BaeGI	GKGCMC	1 cut(s)	8
BanII	GRGCYC	1 cut(s)	8
BarI	GAAGNNNNNNTAC	2 cut(s)	938, 970
BbsI	GAAGAC	2 cut(s)	788, 953
Bbv12I	GWGCWC	1 cut(s)	836
BbvI	GCAGC	2 cut(s)	1433, 1436
BccI	CCATC	3 cut(s)	611, 860, 1384
BceAI	ACGGC	1 cut(s)	426
BciT130I	CCWGG	4 cut(s)	331, 430, 543, 906
BciVI	GTATCC	3 cut(s)	634, 905, 1126
BfaI	CTAG	3 cut(s)	810, 1016, 1581
BfmI	CTRYAG	2 cut(s)	1096, 1212
BfuAI	ACCTGC	1 cut(s)	772
BfuI	GTATCC	3 cut(s)	634, 905, 1126
BisI	GCNGC	2 cut(s)	1422, 1425
BlsI	GCNGC	2 cut(s)	1423, 1426
Bme1390I	CCNGG	4 cut(s)	331, 430, 543, 906
Bme18I	GGWCC	2 cut(s)	895, 929
BmgBI	CACGTC	1 cut(s)	985
BmgT120I	GGNCC	6 cut(s)	4, 5, 813, 826, 895, 929
BmiI	GGNNCC	3 cut(s)	6, 7, 896
BmrFI	CCNGG	4 cut(s)	331, 430, 543, 906
BmrI	ACTGGG	2 cut(s)	517, 823
BmsI	GCATC	2 cut(s)	565, 605
BmtI	GCTAGC	1 cut(s)	1584
BmuI	ACTGGG	2 cut(s)	517, 823
BpiI	GAAGAC	2 cut(s)	788, 953
BplI	GAGNNNNNCTC	2 cut(s)	170, 202
Bsa29I	ATCGAT	1 cut(s)	788
BsaAI	YACGTR	1 cut(s)	1551
BsaJI	CCNNGG	8 cut(s)	329, 330, 428, 565, 713, 851, 1408, 1556
BsaWI	WCCGGW	1 cut(s)	644
BsaXI	ACNNNNNCTCC	6 cut(s)	1028, 1058, 1165, 1195, 1211, 1241
Bsc4I	CCNNNNNNNGG	3 cut(s)	548, 811, 1129
Bse118I	RCCGGY	1 cut(s)	644
Bse1I	ACTGG	4 cut(s)	512, 829, 920, 1535
Bse3DI	GCAATG	2 cut(s)	172, 324
BseBI	CCWGG	4 cut(s)	331, 430, 543, 906
BseCI	ATCGAT	1 cut(s)	788
BseDI	CCNNGG	8 cut(s)	329, 330, 428, 565, 713, 851, 1408, 1556
BseLI	CCNNNNNNNGG	3 cut(s)	548, 811, 1129
BseMI	GCAATG	2 cut(s)	172, 324
BseMII	CTCAG	1 cut(s)	168
BseNI	ACTGG	4 cut(s)	512, 829, 920, 1535
BseRI	GAGGAG	2 cut(s)	771, 1245
BseSI	GKGCMC	1 cut(s)	8
BseXI	GCAGC	2 cut(s)	1433, 1436
BseYI	CCCAGC	2 cut(s)	805, 1032
BsgI	GTGCAG	2 cut(s)	758, 1007
Bsh1236I	CGCG	1 cut(s)	715
BshFI	GGCC	5 cut(s)	6, 433, 441, 815, 827
BshTI	ACCGGT	1 cut(s)	644
BshVI	ATCGAT	1 cut(s)	788
BsiHKAI	GWGCWC	1 cut(s)	836
BsiSI	CCGG	1 cut(s)	645
BslFI	GGGAC	2 cut(s)	731, 1235
BslI	CCNNNNNNNGG	3 cut(s)	548, 811, 1129
BsmFI	GGGAC	2 cut(s)	731, 1235
BsmI	GAATGC	1 cut(s)	1405
BsnI	GGCC	5 cut(s)	6, 433, 441, 815, 827
Bsp120I	GGGCCC	1 cut(s)	4
Bsp1286I	GDGCHC	2 cut(s)	8, 836
Bsp1407I	TGTACA	2 cut(s)	499, 1599
Bsp143I	GATC	1 cut(s)	785
Bsp19I	CCATGG	1 cut(s)	851
BspACI	CCGC	5 cut(s)	111, 713, 715, 970, 1584
BspANI	GGCC	5 cut(s)	6, 433, 441, 815, 827
BspCNI	CTCAG	1 cut(s)	169
BspDI	ATCGAT	1 cut(s)	788
BspFNI	CGCG	1 cut(s)	715
BspHI	TCATGA	2 cut(s)	724, 1525
BspLI	GGNNCC	3 cut(s)	6, 7, 896
BspMAI	CTGCAG	1 cut(s)	1100
BspMI	ACCTGC	1 cut(s)	772
BspOI	GCTAGC	1 cut(s)	1584
BspPI	GGATC	1 cut(s)	793
BsrBI	CCGCTC	1 cut(s)	111
BsrDI	GCAATG	2 cut(s)	172, 324
BsrFI	RCCGGY	1 cut(s)	644
BsrGI	TGTACA	2 cut(s)	499, 1599
BsrI	ACTGG	4 cut(s)	512, 829, 920, 1535
BssAI	RCCGGY	1 cut(s)	644
BssECI	CCNNGG	8 cut(s)	329, 330, 428, 565, 713, 851, 1408, 1556
BssMI	GATC	1 cut(s)	785
BssT1I	CCWWGG	4 cut(s)	565, 851, 1408, 1556
Bst2UI	CCWGG	4 cut(s)	331, 430, 543, 906
Bst4CI	ACNGT	1 cut(s)	487
Bst6I	CTCTTC	2 cut(s)	366, 677
BstAUI	TGTACA	2 cut(s)	499, 1599
BstBAI	YACGTR	1 cut(s)	1551
BstC8I	GCNNGC	3 cut(s)	990, 1405, 1582
BstDEI	CTNAG	3 cut(s)	177, 899, 975
BstDSI	CCRYGG	2 cut(s)	713, 851
BstFNI	CGCG	1 cut(s)	715
BstHHI	GCGC	1 cut(s)	572
BstKTI	GATC	1 cut(s)	788
BstMBI	GATC	1 cut(s)	785
BstMWI	GCNNNNNNNGC	1 cut(s)	994
BstNI	CCWGG	4 cut(s)	331, 430, 543, 906
BstNSI	RCATGY	3 cut(s)	1170, 1334, 1407
BstSCI	CCNGG	4 cut(s)	329, 428, 541, 904
BstSFI	CTRYAG	2 cut(s)	1096, 1212
BstSLI	GKGCMC	1 cut(s)	8
BstSNI	TACGTA	1 cut(s)	1551
BstUI	CGCG	1 cut(s)	715
BstV1I	GCAGC	2 cut(s)	1433, 1436
BstV2I	GAAGAC	2 cut(s)	788, 953
BstXI	CCANNNNNNTGG	2 cut(s)	429, 942
Bsu15I	ATCGAT	1 cut(s)	788
BsuI	GTATCC	3 cut(s)	634, 905, 1126
BsuRI	GGCC	5 cut(s)	6, 433, 441, 815, 827
BsuTUI	ATCGAT	1 cut(s)	788
BtgI	CCRYGG	2 cut(s)	713, 851
BtrI	CACGTC	1 cut(s)	985
BtsIMutI	CAGTG	1 cut(s)	836
BveI	ACCTGC	1 cut(s)	772
Cac8I	GCNNGC	3 cut(s)	990, 1405, 1582
CciI	TCATGA	2 cut(s)	724, 1525
CfoI	GCGC	1 cut(s)	572
Cfr10I	RCCGGY	1 cut(s)	644
Cfr13I	GGNCC	6 cut(s)	4, 5, 813, 826, 895, 929
Cfr42I	CCGCGG	1 cut(s)	716
ClaI	ATCGAT	1 cut(s)	788
CsiI	ACCWGGT	1 cut(s)	904
Csp6I	GTAC	7 cut(s)	467, 500, 761, 874, 1150, 1552, 1600
CspAI	ACCGGT	1 cut(s)	644
CviQI	GTAC	7 cut(s)	467, 500, 761, 874, 1150, 1552, 1600
DdeI	CTNAG	3 cut(s)	177, 899, 975
DpnI	GATC	1 cut(s)	787
DpnII	GATC	1 cut(s)	785
Eam1104I	CTCTTC	2 cut(s)	366, 677
EarI	CTCTTC	2 cut(s)	366, 677
Eco105I	TACGTA	1 cut(s)	1551
Eco130I	CCWWGG	4 cut(s)	565, 851, 1408, 1556
Eco24I	GRGCYC	1 cut(s)	8
Eco32I	GATATC	1 cut(s)	1379
Eco47I	GGWCC	2 cut(s)	895, 929
Eco57I	CTGAAG	1 cut(s)	6
EcoO109I	RGGNCCY	3 cut(s)	5, 813, 895
EcoRII	CCWGG	4 cut(s)	329, 428, 541, 904
EcoRV	GATATC	1 cut(s)	1379
EcoT14I	CCWWGG	4 cut(s)	565, 851, 1408, 1556
EcoT22I	ATGCAT	2 cut(s)	580, 1405
EcoT38I	GRGCYC	1 cut(s)	8
ErhI	CCWWGG	4 cut(s)	565, 851, 1408, 1556
FaqI	GGGAC	2 cut(s)	731, 1235
FauI	CCCGC	1 cut(s)	708
FauNDI	CATATG	1 cut(s)	1564
Fnu4HI	GCNGC	2 cut(s)	1422, 1425
FriOI	GRGCYC	1 cut(s)	8
Fsp4HI	GCNGC	2 cut(s)	1422, 1425
FspBI	CTAG	3 cut(s)	810, 1016, 1581
GlaI	GCGC	1 cut(s)	571
GluI	GCNGC	2 cut(s)	1422, 1425
GsaI	CCCAGC	2 cut(s)	809, 1036
HaeIII	GGCC	5 cut(s)	6, 433, 441, 815, 827
HapII	CCGG	1 cut(s)	645
HhaI	GCGC	1 cut(s)	572
Hin6I	GCGC	1 cut(s)	570
HinP1I	GCGC	1 cut(s)	570
HinfI	GANTC	4 cut(s)	418, 790, 1350, 1433
HpaII	CCGG	1 cut(s)	645
HphI	GGTGA	1 cut(s)	137
Hpy166II	GTNNAC	3 cut(s)	365, 454, 1342
Hpy188I	TCNGA	4 cut(s)	190, 241, 609, 1383
Hpy188III	TCNNGA	9 cut(s)	24, 302, 628, 725, 927, 1069, 1184, 1437, 1526
Hpy8I	GTNNAC	3 cut(s)	365, 454, 1342
HpyAV	CCTTC	5 cut(s)	98, 246, 401, 1188, 1532
HpyCH4III	ACNGT	1 cut(s)	487
HpyCH4IV	ACGT	4 cut(s)	962, 984, 1188, 1550
HpyF10VI	GCNNNNNNNGC	1 cut(s)	994
HpyF3I	CTNAG	3 cut(s)	177, 899, 975
HpySE526I	ACGT	4 cut(s)	962, 984, 1188, 1550
HspAI	GCGC	1 cut(s)	570
KspI	CCGCGG	1 cut(s)	716
Kzo9I	GATC	1 cut(s)	785
LmnI	GCTCC	2 cut(s)	95, 751
Lsp1109I	GCAGC	2 cut(s)	1433, 1436
LweI	GCATC	2 cut(s)	565, 605
MabI	ACCWGGT	1 cut(s)	904
MaeI	CTAG	3 cut(s)	810, 1016, 1581
MaeII	ACGT	4 cut(s)	962, 984, 1188, 1550
MaeIII	GTNAC	5 cut(s)	145, 443, 1171, 1189, 1285
MalI	GATC	1 cut(s)	787
MbiI	CCGCTC	1 cut(s)	111
MboI	GATC	1 cut(s)	785
MboII	GAAGA	7 cut(s)	12, 73, 383, 694, 788, 958, 1084
MfeI	CAATTG	1 cut(s)	1470
MhlI	GDGCHC	2 cut(s)	8, 836
MlyI	GAGTC	1 cut(s)	412
Mph1103I	ATGCAT	2 cut(s)	580, 1405
MseI	TTAA	3 cut(s)	845, 939, 1083
MslI	CAYNNNNRTG	2 cut(s)	213, 1335
MspA1I	CMGCKG	1 cut(s)	715
MspI	CCGG	1 cut(s)	645
MspR9I	CCNGG	4 cut(s)	331, 430, 543, 906
MunI	CAATTG	1 cut(s)	1470
Mva1269I	GAATGC	1 cut(s)	1405
MvaI	CCWGG	4 cut(s)	331, 430, 543, 906
MvnI	CGCG	1 cut(s)	715
MwoI	GCNNNNNNNGC	1 cut(s)	994
NcoI	CCATGG	1 cut(s)	851
NdeI	CATATG	1 cut(s)	1564
NdeII	GATC	1 cut(s)	785
NheI	GCTAGC	1 cut(s)	1580
NlaIV	GGNNCC	3 cut(s)	6, 7, 896
NmuCI	GTSAC	1 cut(s)	443
NsiI	ATGCAT	2 cut(s)	580, 1405
NspI	RCATGY	3 cut(s)	1170, 1334, 1407
PaeI	GCATGC	1 cut(s)	1407
PagI	TCATGA	2 cut(s)	724, 1525
PasI	CCCWGGG	1 cut(s)	330
PciI	ACATGT	1 cut(s)	1166
PctI	GAATGC	1 cut(s)	1405
PfeI	GAWTC	3 cut(s)	790, 1350, 1433
PflFI	GACNNNGTC	2 cut(s)	360, 449
PinAI	ACCGGT	1 cut(s)	644
PkrI	GCNGC	2 cut(s)	1423, 1426
PleI	GAGTC	1 cut(s)	412
PpsI	GAGTC	1 cut(s)	412
Ppu21I	YACGTR	1 cut(s)	1551
PpuMI	RGGWCCY	1 cut(s)	895
PscI	ACATGT	1 cut(s)	1166
PshBI	ATTAAT	1 cut(s)	939
PsiI	TTATAA	1 cut(s)	474
Psp5II	RGGWCCY	1 cut(s)	895
Psp6I	CCWGG	4 cut(s)	329, 428, 541, 904
PspFI	CCCAGC	2 cut(s)	805, 1032
PspGI	CCWGG	4 cut(s)	329, 428, 541, 904
PspN4I	GGNNCC	3 cut(s)	6, 7, 896
PspOMI	GGGCCC	1 cut(s)	4
PspPI	GGNCC	6 cut(s)	4, 5, 813, 826, 895, 929
PspPPI	RGGWCCY	1 cut(s)	895
PstI	CTGCAG	1 cut(s)	1100
PsyI	GACNNNGTC	2 cut(s)	360, 449
RsaI	GTAC	7 cut(s)	468, 501, 762, 875, 1151, 1553, 1601
RsaNI	GTAC	7 cut(s)	467, 500, 761, 874, 1150, 1552, 1600
RseI	CAYNNNNRTG	2 cut(s)	213, 1335
SacII	CCGCGG	1 cut(s)	716
SaqAI	TTAA	3 cut(s)	845, 939, 1083
SatI	GCNGC	2 cut(s)	1422, 1425
Sau3AI	GATC	1 cut(s)	785
Sau96I	GGNCC	6 cut(s)	4, 5, 813, 826, 895, 929
SbfI	CCTGCAGG	1 cut(s)	1100
SchI	GAGTC	1 cut(s)	412
ScrFI	CCNGG	4 cut(s)	331, 430, 543, 906
SdaI	CCTGCAGG	1 cut(s)	1100
SduI	GDGCHC	2 cut(s)	8, 836
SexAI	ACCWGGT	1 cut(s)	904
SfaNI	GCATC	2 cut(s)	565, 605
SfcI	CTRYAG	2 cut(s)	1096, 1212
Sfr303I	CCGCGG	1 cut(s)	716
SgrBI	CCGCGG	1 cut(s)	716
SinI	GGWCC	2 cut(s)	895, 929
SmiMI	CAYNNNNRTG	2 cut(s)	213, 1335
SnaBI	TACGTA	1 cut(s)	1551
SphI	GCATGC	1 cut(s)	1407
Sse8387I	CCTGCAGG	1 cut(s)	1100
SsiI	CCGC	5 cut(s)	111, 713, 715, 970, 1584
SspMI	CTAG	3 cut(s)	810, 1016, 1581
StyD4I	CCNGG	4 cut(s)	329, 428, 541, 904
StyI	CCWWGG	4 cut(s)	565, 851, 1408, 1556
TaaI	ACNGT	1 cut(s)	487
TaiI	ACGT	4 cut(s)	965, 987, 1191, 1553
TaqI	TCGA	3 cut(s)	788, 1113, 1258
TaqII	GACCGA	1 cut(s)	327
TatI	WGTACW	2 cut(s)	499, 1599
TfiI	GAWTC	3 cut(s)	790, 1350, 1433
Tru1I	TTAA	3 cut(s)	845, 939, 1083
Tru9I	TTAA	3 cut(s)	845, 939, 1083
TscAI	CASTG	1 cut(s)	836
TseFI	GTSAC	1 cut(s)	443
TseI	GCWGC	2 cut(s)	1421, 1424
Tsp45I	GTSAC	1 cut(s)	443
TspDTI	ATGAA	7 cut(s)	335, 603, 741, 1218, 1412, 1469, 1565
TspRI	CASTG	1 cut(s)	836
Tth111I	GACNNNGTC	2 cut(s)	360, 449
VpaK11BI	GGWCC	2 cut(s)	895, 929
VspI	ATTAAT	1 cut(s)	939
XapI	RAATTY	2 cut(s)	323, 536
XceI	RCATGY	3 cut(s)	1170, 1334, 1407
XspI	CTAG	3 cut(s)	810, 1016, 1581
Zsp2I	ATGCAT	2 cut(s)	580, 1405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.