FvH4_5g28150

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016861
Physical Location & Seq
Forward (+)
19343751 .. 19345656
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28150.t1

Sequence Viewer

Length: 1218 bp
ATGTTCGGGGAAAACCGCTCTACTCTTTGTTCATTCATTTTTCTTGAACCGACAACAAATTTCGATACTCTACAATCTTGGTTTTTACTCTGCACGCATTTTGATTTAGATAGAAGACGTCCGCTAGTACTTGCATATTTTGAATTGGGTTTACTTTGTGCCCAATTCAAGATATCAGTGTTTGTTTTCGAAGAAATGATGGGCAGAAATGATGAATGCACATCTCCTCTGCCTGGTGAATGGATGAAGGGCAAATTGGTTGGATCAGGTTCTTTTGGAAGTGTCCATTTGGCCATGAGCAAATCCACAGGAGGACTTTTCGTTGTGAAATCCTCGCAGTCTTGTGATGGAGCCCTCGCTCTTGAGAATGAGGCTAAGATTCTCGAGAGTTTGAATTCCCCATATGTTGTTCGATGTATAGGGATCGAAAATGGTAGAGGGAAATTGTGCAATGTTTTCATGGAGTACATGGCAGGGGGTAGTTTGTCAGATGTGGCTCAGATATTTGGTGGGGCTTTCGATGAAGACATGGTTCGCTTGTACTCGAGAGAGATTCTTCATGGTCTCAAGTATCTTCACGAAAATGGGATTATGCATTGTGATCTCAAGTGCAAGAATGTGCTCTTGAGCTCTTCCGGGAATGTGAAGCTGGCAGACTTTGGTTGCGCTAGGAGGCTCAAGGACTCGACCACCACTACTGGTGGAACTCCATTATGGATGGCTCCCGAAGTTTTGAGGAATGAAGGGCTGGATTTTCCTTGTGACATTTGGTCATTGGGATGCACCGTGATTGAAATGGCCACCGGAAGGCCTCCTTGGGCCGCTGAGATTTCTGATCCGGTAGCTGCTGTTCTGAAGATTGCTTGCAGCAGCGAGAGGCCTCAGTTTCCGAGACAGTTTTCAAATGAGGGGTCGGATTTCTTAGCGAAATGCTTGGAGAGAGACCCGAAAAGGAGGTGGTCTGCTGAGCAACTACTTAACCATCCTTTTGTCTCTGGAAATCCAATGAGAGTTTCAAGAAAGGGCGAGACATGCTCACCGGCAAGTACTTTGGATGACATTGGAATGTACAATTTTGATTGTGGAATTGAGAGCTGTGAGGGCAATGAGTTCGCCTTCAGAAATCCTTTCTCGAGGTGTTGTGACGAAATAAACAGAAATACAGTAAAGCCTGAGTGTCACCTTGAGTCATCTGAAAATTGGATCACTGTTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

406

Amino Acids

45.08

Weight (kDa)

5.36

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 83 - 331 6.3e-65 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 85 - 327 4.3e-39 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 153 - 230 4.4e-07 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016861)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g28150
malus_domestica MD08G1245500.v1.1 MD15G1440500.v1.1
prunus_persica Prupe.1G582600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220281
rosa_laevigata RLG00000002243
rosa_multiflora Rmu_sc0001136.1_g000029
rosa_roxburghii Rroxscaffold_3G00239650
rosa_rugosa Rorug07G0191800
rosa_samantha Rh7AG333800 Rh7BG323300 Rh7CG351400 Rh7DG330100
rosa_wichuraiana Rw7G028160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 121
AccBSI CCGCTC 1 cut(s) 18
AciI CCGC 3 cut(s) 16, 122, 822
AclWI GGATC 4 cut(s) 271, 431, 830, 1211
AcoI YGGCCR 2 cut(s) 291, 798
AcsI RAATTY 2 cut(s) 58, 394
AcuI CTGAAG 2 cut(s) 875, 1102
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 5 cut(s) 129, 467, 542, 1048, 1070
AfiI CCNNNNNNNGG 2 cut(s) 233, 807
AgsI TTSAA 7 cut(s) 47, 143, 169, 394, 794, 903, 1017
AhdI GACNNNNNGTC 1 cut(s) 769
AjnI CCWGG 1 cut(s) 232
AjuI GAANNNNNNNTTGG 6 cut(s) 239, 271, 799, 831, 997, 1029
AluBI AGCT 4 cut(s) 630, 649, 845, 1095
AluI AGCT 4 cut(s) 630, 649, 845, 1095
Alw21I GWGCWC 2 cut(s) 624, 632
Alw26I GTCTC 5 cut(s) 569, 886, 936, 997, 1022
AlwI GGATC 4 cut(s) 271, 431, 830, 1211
Ama87I CYCGRG 3 cut(s) 383, 544, 1132
AoxI GGCC 5 cut(s) 291, 798, 809, 819, 878
ApeKI GCWGC 3 cut(s) 845, 867, 870
ApoI RAATTY 2 cut(s) 58, 394
ArsI GACNNNNNNTTYG 2 cut(s) 896, 928
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 1 cut(s) 819
AsuC2I CCSGG 1 cut(s) 637
AsuHPI GGTGA 3 cut(s) 248, 1029, 1172
AsuII TTCGAA 1 cut(s) 189
AvaI CYCGRG 3 cut(s) 383, 544, 1132
BaeGI GKGCMC 1 cut(s) 163
BalI TGGCCA 2 cut(s) 293, 800
BanII GRGCYC 2 cut(s) 355, 632
BbsI GAAGAC 2 cut(s) 121, 531
Bbv12I GWGCWC 2 cut(s) 624, 632
BbvI GCAGC 3 cut(s) 832, 879, 882
BccI CCATC 4 cut(s) 193, 341, 712, 990
BcgI CGANNNNNNTGC 2 cut(s) 1093, 1127
BciT130I CCWGG 1 cut(s) 234
BcnI CCSGG 1 cut(s) 637
BcoDI GTCTC 5 cut(s) 569, 886, 936, 997, 1022
BfaI CTAG 2 cut(s) 125, 669
BisI GCNGC 4 cut(s) 822, 846, 868, 871
BlpI GCTNAGC 1 cut(s) 966
BlsI GCNGC 4 cut(s) 823, 847, 869, 872
BmcAI AGTACT 2 cut(s) 129, 1048
Bme1390I CCNGG 2 cut(s) 234, 637
BmeRI GACNNNNNGTC 1 cut(s) 769
BmeT110I CYCGRG 3 cut(s) 383, 544, 1132
BmgT120I GGNCC 1 cut(s) 819
BmiI GGNNCC 2 cut(s) 352, 723
BmrFI CCNGG 2 cut(s) 234, 637
BmsI GCATC 1 cut(s) 770
BpiI GAAGAC 2 cut(s) 121, 531
BplI GAGNNNNNCTC 2 cut(s) 1019, 1051
Bpu1102I GCTNAGC 1 cut(s) 966
Bpu14I TTCGAA 1 cut(s) 189
BpuEI CTTGAG 6 cut(s) 383, 551, 590, 646, 662, 1205
BpuMI CCSGG 1 cut(s) 637
BsaHI GRCGYC 1 cut(s) 118
BsaI GGTCTC 2 cut(s) 569, 936
BsaJI CCNNGG 1 cut(s) 815
BsaWI WCCGGW 2 cut(s) 803, 838
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 807
Bse118I RCCGGY 1 cut(s) 1039
Bse1I ACTGG 1 cut(s) 703
Bse3DI GCAATG 2 cut(s) 457, 1111
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 815
BseGI GGATG 5 cut(s) 249, 723, 785, 982, 1060
BseLI CCNNNNNNNGG 2 cut(s) 233, 807
BseMI GCAATG 2 cut(s) 457, 1111
BseMII CTCAG 5 cut(s) 512, 816, 896, 957, 1164
BseNI ACTGG 1 cut(s) 703
BseRI GAGGAG 1 cut(s) 216
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 3 cut(s) 832, 879, 882
BsgI GTGCAG 1 cut(s) 76
BshFI GGCC 5 cut(s) 293, 800, 811, 821, 880
BsiHKAI GWGCWC 2 cut(s) 624, 632
BsiHKCI CYCGRG 3 cut(s) 383, 544, 1132
BsiSI CCGG 4 cut(s) 636, 804, 839, 1040
BslI CCNNNNNNNGG 2 cut(s) 233, 807
BsmAI GTCTC 5 cut(s) 569, 886, 936, 997, 1022
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 5 cut(s) 293, 800, 811, 821, 880
Bso31I GGTCTC 2 cut(s) 569, 936
BsoBI CYCGRG 3 cut(s) 383, 544, 1132
Bsp119I TTCGAA 1 cut(s) 189
Bsp1286I GDGCHC 4 cut(s) 163, 355, 624, 632
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 5 cut(s) 263, 423, 601, 835, 1203
Bsp1720I GCTNAGC 1 cut(s) 966
BspACI CCGC 3 cut(s) 16, 122, 822
BspANI GGCC 5 cut(s) 293, 800, 811, 821, 880
BspCNI CTCAG 5 cut(s) 511, 817, 895, 958, 1165
BspLI GGNNCC 2 cut(s) 352, 723
BspPI GGATC 4 cut(s) 271, 431, 830, 1211
BspQI GCTCTTC 1 cut(s) 637
BspT104I TTCGAA 1 cut(s) 189
BspTNI GGTCTC 2 cut(s) 569, 936
BsrBI CCGCTC 1 cut(s) 18
BsrDI GCAATG 2 cut(s) 457, 1111
BsrFI RCCGGY 1 cut(s) 1039
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 1 cut(s) 703
BssAI RCCGGY 1 cut(s) 1039
BssECI CCNNGG 1 cut(s) 815
BssMI GATC 5 cut(s) 263, 423, 601, 835, 1203
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 815
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 4 cut(s) 787, 897, 1165, 1210
Bst6I CTCTTC 1 cut(s) 637
BstACI GRCGYC 1 cut(s) 118
BstAUI TGTACA 1 cut(s) 1068
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 95, 651, 865
BstDEI CTNAG 7 cut(s) 375, 498, 825, 882, 922, 966, 1173
BstF5I GGATG 5 cut(s) 249, 723, 785, 982, 1060
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 5 cut(s) 266, 426, 604, 838, 1206
BstMAI GTCTC 5 cut(s) 569, 886, 936, 997, 1022
BstMBI GATC 5 cut(s) 263, 423, 601, 835, 1203
BstMWI GCNNNNNNNGC 2 cut(s) 1032, 1101
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 1035
BstSCI CCNGG 2 cut(s) 232, 635
BstSLI GKGCMC 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 832, 879, 882
BstV2I GAAGAC 2 cut(s) 121, 531
BsuRI GGCC 5 cut(s) 293, 800, 811, 821, 880
BtsCI GGATG 5 cut(s) 249, 723, 785, 982, 1060
BtsIMutI CAGTG 2 cut(s) 183, 1206
Cac8I GCNNGC 3 cut(s) 95, 651, 865
CfoI GCGC 1 cut(s) 668
Cfr10I RCCGGY 1 cut(s) 1039
Cfr13I GGNCC 1 cut(s) 819
Csp6I GTAC 5 cut(s) 128, 466, 541, 1047, 1069
CviAII CATG 6 cut(s) 295, 460, 469, 529, 560, 1032
CviQI GTAC 5 cut(s) 128, 466, 541, 1047, 1069
DdeI CTNAG 7 cut(s) 375, 498, 825, 882, 922, 966, 1173
DpnI GATC 5 cut(s) 265, 425, 603, 837, 1205
DpnII GATC 5 cut(s) 263, 423, 601, 835, 1203
DriI GACNNNNNGTC 1 cut(s) 769
EaeI YGGCCR 2 cut(s) 291, 798
Eam1104I CTCTTC 1 cut(s) 637
Eam1105I GACNNNNNGTC 1 cut(s) 769
EarI CTCTTC 1 cut(s) 637
Ecl136II GAGCTC 1 cut(s) 630
Eco130I CCWWGG 1 cut(s) 815
Eco147I AGGCCT 2 cut(s) 811, 880
Eco24I GRGCYC 2 cut(s) 355, 632
Eco31I GGTCTC 2 cut(s) 569, 936
Eco32I GATATC 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 2 cut(s) 875, 1102
Eco88I CYCGRG 3 cut(s) 383, 544, 1132
EcoICRI GAGCTC 1 cut(s) 630
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 232
EcoRV GATATC 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 815
EcoT22I ATGCAT 1 cut(s) 597
EcoT38I GRGCYC 2 cut(s) 355, 632
ErhI CCWWGG 1 cut(s) 815
FaeI CATG 6 cut(s) 298, 463, 472, 532, 563, 1035
FalI AAGNNNNNCTT 2 cut(s) 799, 831
FatI CATG 6 cut(s) 294, 459, 468, 528, 559, 1031
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 4 cut(s) 822, 846, 868, 871
FokI GGATG 5 cut(s) 256, 730, 792, 969, 1067
FriOI GRGCYC 2 cut(s) 355, 632
Fsp4HI GCNGC 4 cut(s) 822, 846, 868, 871
FspBI CTAG 2 cut(s) 125, 669
GlaI GCGC 1 cut(s) 667
GluI GCNGC 4 cut(s) 822, 846, 868, 871
HaeIII GGCC 5 cut(s) 293, 800, 811, 821, 880
HapII CCGG 4 cut(s) 636, 804, 839, 1040
HhaI GCGC 1 cut(s) 668
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 6 cut(s) 298, 463, 472, 532, 563, 1035
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HinfI GANTC 4 cut(s) 379, 553, 683, 1187
HpaII CCGG 4 cut(s) 636, 804, 839, 1040
HphI GGTGA 3 cut(s) 248, 1029, 1172
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 8 cut(s) 490, 501, 835, 855, 891, 916, 1121, 1195
Hpy8I GTNNAC 1 cut(s) 152
HpyAV CCTTC 4 cut(s) 241, 737, 801, 1126
HpyCH4III ACNGT 4 cut(s) 787, 897, 1165, 1210
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 8 cut(s) 93, 134, 219, 450, 595, 612, 783, 867
HpyF10VI GCNNNNNNNGC 2 cut(s) 1032, 1101
HpyF3I CTNAG 7 cut(s) 375, 498, 825, 882, 922, 966, 1173
HpySE526I ACGT 1 cut(s) 118
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 6 cut(s) 298, 463, 472, 532, 563, 1035
HspAI GCGC 1 cut(s) 666
Kzo9I GATC 5 cut(s) 263, 423, 601, 835, 1203
LguI GCTCTTC 1 cut(s) 637
LmnI GCTCC 2 cut(s) 350, 727
Lsp1109I GCAGC 3 cut(s) 832, 879, 882
LweI GCATC 1 cut(s) 770
MaeI CTAG 2 cut(s) 125, 669
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 3 cut(s) 761, 1142, 1178
MalI GATC 5 cut(s) 265, 425, 603, 837, 1205
MbiI CCGCTC 1 cut(s) 18
MboI GATC 5 cut(s) 263, 423, 601, 835, 1203
MboII GAAGA 7 cut(s) 126, 203, 536, 548, 566, 624, 868
MhlI GDGCHC 4 cut(s) 163, 355, 624, 632
MlsI TGGCCA 2 cut(s) 293, 800
MluCI AATT 9 cut(s) 58, 143, 164, 254, 394, 443, 1072, 1086, 1198
MluNI TGGCCA 2 cut(s) 293, 800
MlyI GAGTC 2 cut(s) 677, 1196
MmeI TCCRAC 2 cut(s) 241, 894
Mox20I TGGCCA 2 cut(s) 293, 800
Mph1103I ATGCAT 1 cut(s) 597
MscI TGGCCA 2 cut(s) 293, 800
MseI TTAA 1 cut(s) 978
MslI CAYNNNNRTG 3 cut(s) 582, 778, 1064
Msp20I TGGCCA 2 cut(s) 293, 800
MspA1I CMGCKG 1 cut(s) 824
MspI CCGG 4 cut(s) 636, 804, 839, 1040
MspR9I CCNGG 2 cut(s) 234, 637
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 2 cut(s) 1032, 1101
NciI CCSGG 1 cut(s) 637
NdeI CATATG 1 cut(s) 403
NdeII GATC 5 cut(s) 263, 423, 601, 835, 1203
NlaIII CATG 6 cut(s) 298, 463, 472, 532, 563, 1035
NlaIV GGNNCC 2 cut(s) 352, 723
NmuCI GTSAC 3 cut(s) 761, 1142, 1178
NsiI ATGCAT 1 cut(s) 597
NspI RCATGY 1 cut(s) 1035
NspV TTCGAA 1 cut(s) 189
PaeR7I CTCGAG 3 cut(s) 383, 544, 1132
PceI AGGCCT 2 cut(s) 811, 880
PciSI GCTCTTC 1 cut(s) 637
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 379, 553
PfoI TCCNGGA 1 cut(s) 635
PkrI GCNGC 4 cut(s) 823, 847, 869, 872
PleI GAGTC 2 cut(s) 677, 1195
PpsI GAGTC 2 cut(s) 677, 1195
Psp124BI GAGCTC 1 cut(s) 632
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 352, 723
PspPI GGNCC 1 cut(s) 819
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
RsaI GTAC 5 cut(s) 129, 467, 542, 1048, 1070
RsaNI GTAC 5 cut(s) 128, 466, 541, 1047, 1069
RseI CAYNNNNRTG 3 cut(s) 582, 778, 1064
SacI GAGCTC 1 cut(s) 632
SapI GCTCTTC 1 cut(s) 637
SaqAI TTAA 1 cut(s) 978
SatI GCNGC 4 cut(s) 822, 846, 868, 871
Sau3AI GATC 5 cut(s) 263, 423, 601, 835, 1203
Sau96I GGNCC 1 cut(s) 819
ScaI AGTACT 2 cut(s) 129, 1048
SchI GAGTC 2 cut(s) 677, 1196
ScrFI CCNGG 2 cut(s) 234, 637
SduI GDGCHC 4 cut(s) 163, 355, 624, 632
SetI ASST 9 cut(s) 121, 271, 632, 651, 847, 959, 1097, 1139, 1185
SfaNI GCATC 1 cut(s) 770
Sfr274I CTCGAG 3 cut(s) 383, 544, 1132
SfuI TTCGAA 1 cut(s) 189
SlaI CTCGAG 3 cut(s) 383, 544, 1132
SmiMI CAYNNNNRTG 3 cut(s) 582, 778, 1064
SmlI CTYRAG 9 cut(s) 362, 383, 544, 566, 605, 625, 677, 1132, 1184
SmoI CTYRAG 9 cut(s) 362, 383, 544, 566, 605, 625, 677, 1132, 1184
Sse9I AATT 9 cut(s) 58, 143, 164, 254, 394, 443, 1072, 1086, 1198
SseBI AGGCCT 2 cut(s) 811, 880
SsiI CCGC 3 cut(s) 16, 122, 822
SspMI CTAG 2 cut(s) 125, 669
SstI GAGCTC 1 cut(s) 632
StuI AGGCCT 2 cut(s) 811, 880
StyD4I CCNGG 2 cut(s) 232, 635
StyI CCWWGG 1 cut(s) 815
TaaI ACNGT 4 cut(s) 787, 897, 1165, 1210
TaiI ACGT 1 cut(s) 121
TaqI TCGA 9 cut(s) 63, 189, 384, 412, 426, 519, 545, 686, 1133
TasI AATT 9 cut(s) 58, 143, 164, 254, 394, 443, 1072, 1086, 1198
TatI WGTACW 5 cut(s) 127, 465, 540, 1046, 1068
TauI GCSGC 1 cut(s) 824
TfiI GAWTC 2 cut(s) 379, 553
Tru1I TTAA 1 cut(s) 978
Tru9I TTAA 1 cut(s) 978
TscAI CASTG 2 cut(s) 183, 1213
TseFI GTSAC 3 cut(s) 761, 1142, 1178
TseI GCWGC 3 cut(s) 845, 867, 870
Tsp45I GTSAC 3 cut(s) 761, 1142, 1178
TspDTI ATGAA 8 cut(s) 21, 25, 228, 260, 448, 537, 548, 756
TspRI CASTG 2 cut(s) 183, 1213
XapI RAATTY 2 cut(s) 58, 394
XceI RCATGY 1 cut(s) 1035
XhoI CTCGAG 3 cut(s) 383, 544, 1132
XspI CTAG 2 cut(s) 125, 669
ZraI GACGTC 1 cut(s) 119
ZrmI AGTACT 2 cut(s) 129, 1048
Zsp2I ATGCAT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.