MD08G1245500.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016861
Physical Location & Seq
Forward (+)
31011902 .. 31012954
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1245500.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCTGCGAATCCTGATCAGTGCACATCTCCTCTGCCGGGTGAGTGGATGAAGGGAAAAATGGTCGGTTCTGGTTCTTATGGGAGTGTTCATCTGGCCATGAACAAAGCCACAGGAGCGCTTTTCGTTGTGAAATCTGCTCAGTCTAGGGATGGAGCTGAGGCTCTTGAGAACGAGGTTAAGATTCTCGAGAGTATGAATTCCCCACATGTTGTTAAATGTATGGGGAGGGAAAATGGTGAAGGGAAATGCACTAATGTGTTCATGGAGTACATGGCAGGGGGTAGTTTGTCAGATGTGATGCAGATATTTGGCGGGGCGTTGGAGGAAGAAGTGGTTCGGTTGTACACAAGGGAGATTCTTCACGGCCTCAAGTATCTTCATGAAAGTGGGATTGTGCATTGTGACCTCAAGTGCAGCAATGTGCTCTTGGGATCCTCCGGGAATGTCAAGTTGGCGGATTTTGGCTTCGCAAAGAGGCTAAAGGCCTCAAAGGCTGACGGGGTTTCAGTGCAGCACTCTTTGCAATGTATTGGCGGGACTCCATTGTGGATGGCTCCTGAAGTTTTGAGAAACGAAGGGCTAGATTTTGCTTCGGATATCTGGTCATTGGGATGCACGGTGATTGAAATGCTTACCGGGAGGCCTCCTTGGGGCAATGAGATTTGGAATCCGGTTGCTGCTGTTTTGAAGATTGCTTGTGGCAATGAGAAGCCTCAGTTCCCGCAAAAGCTTTCGGACGAAGGGTTGGATTTCTTAGCAAGATGCTTGGAGAGAGACCCCAAAAGGAGGTGGTCTGCTAAGGAGTTGCTCAACCATCCATTTGTGTCTGGAAACTCGATGCAATTTTCAAGAAATGGTGCGGCCTACTCACCAGCGAGCACATTAGACATTGGACTTTACGAAGAGGGTTCTGATTGTTCAGATCAGGGATTTGAGAGCTGCGATCGCGATGAGTTCCCCAGCAGCCCAAGTAGAAACCCTTTTTCAAGAAGGCAGCAGATATCTGAGAATCACTTGGAGTCGTCAGGAAATTGGATTACTGTTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

351

Amino Acids

38.27

Weight (kDa)

5.58

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 18 - 276 1.7e-65 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 19 - 272 1.1e-40 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 88 - 164 1e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016861)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g28150
malus_domestica MD08G1245500.v1.1 MD15G1440500.v1.1
prunus_persica Prupe.1G582600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220281
rosa_laevigata RLG00000002243
rosa_multiflora Rmu_sc0001136.1_g000029
rosa_roxburghii Rroxscaffold_3G00239650
rosa_rugosa Rorug07G0191800
rosa_samantha Rh7AG333800 Rh7BG323300 Rh7CG351400 Rh7DG330100
rosa_wichuraiana Rw7G028160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 951
AciI CCGC 5 cut(s) 315, 458, 537, 725, 863
AclWI GGATC 2 cut(s) 429, 442
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 582
AfaI GTAC 2 cut(s) 272, 347
AfeI AGCGCT 1 cut(s) 120
AfiI CCNNNNNNNGG 3 cut(s) 38, 653, 789
AflIII ACRYGT 1 cut(s) 208
AgsI TTSAA 4 cut(s) 629, 691, 852, 990
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 3 cut(s) 158, 733, 942
AluI AGCT 3 cut(s) 158, 733, 942
Alw21I GWGCWC 3 cut(s) 26, 429, 884
Alw26I GTCTC 1 cut(s) 771
Alw44I GTGCAC 1 cut(s) 22
AlwI GGATC 2 cut(s) 429, 442
Ama87I CYCGRG 1 cut(s) 188
Aor51HI AGCGCT 1 cut(s) 120
AoxI GGCC 5 cut(s) 96, 367, 486, 644, 864
ApaLI GTGCAC 1 cut(s) 22
ApeKI GCWGC 7 cut(s) 5, 417, 514, 680, 942, 966, 997
ApoI RAATTY 1 cut(s) 199
AsiSI GCGATCGC 1 cut(s) 949
Asp700I GAANNNNTTC 1 cut(s) 336
AspLEI GCGC 1 cut(s) 121
AsuC2I CCSGG 3 cut(s) 39, 442, 640
AsuHPI GGTGA 4 cut(s) 53, 251, 634, 864
AvaI CYCGRG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 26
BalI TGGCCA 1 cut(s) 98
BamHI GGATCC 1 cut(s) 434
Bbv12I GWGCWC 3 cut(s) 26, 429, 884
BbvCI CCTCAGC 1 cut(s) 159
BbvI GCAGC 6 cut(s) 429, 526, 667, 929, 978, 1009
BccI CCATC 3 cut(s) 146, 547, 825
BceAI ACGGC 1 cut(s) 382
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 3 cut(s) 39, 442, 640
BcoDI GTCTC 1 cut(s) 771
BfaI CTAG 2 cut(s) 147, 584
BfoI RGCGCY 1 cut(s) 122
BglI GCCNNNNNGGC 1 cut(s) 494
BisI GCNGC 8 cut(s) 6, 418, 515, 681, 864, 943, 967, 998
BlsI GCNGC 8 cut(s) 7, 419, 516, 682, 865, 944, 968, 999
Bme1390I CCNGG 3 cut(s) 39, 442, 640
BmeT110I CYCGRG 1 cut(s) 188
BmiI GGNNCC 2 cut(s) 436, 558
BmrFI CCNGG 3 cut(s) 39, 442, 640
BmsI GCATC 4 cut(s) 291, 605, 755, 831
Bpu10I CCTNAGC 2 cut(s) 159, 801
BpuEI CTTGAG 3 cut(s) 188, 356, 395
BpuMI CCSGG 3 cut(s) 39, 442, 640
BsaI GGTCTC 1 cut(s) 771
BsaJI CCNNGG 1 cut(s) 650
BsaWI WCCGGW 1 cut(s) 673
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 653, 789
Bse3DI GCAATG 4 cut(s) 427, 533, 664, 712
BseDI CCNNGG 1 cut(s) 650
BseGI GGATG 5 cut(s) 54, 157, 558, 620, 817
BseLI CCNNNNNNNGG 3 cut(s) 38, 653, 789
BseMI GCAATG 4 cut(s) 427, 533, 664, 712
BseMII CTCAG 4 cut(s) 150, 155, 731, 999
BseRI GAGGAG 1 cut(s) 21
BseSI GKGCMC 1 cut(s) 26
BseXI GCAGC 6 cut(s) 429, 526, 667, 929, 978, 1009
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 2 cut(s) 436, 533
Bsh1236I CGCG 1 cut(s) 951
Bsh1285I CGRYCG 1 cut(s) 949
BshFI GGCC 5 cut(s) 98, 369, 488, 646, 866
BsiEI CGRYCG 1 cut(s) 949
BsiHKAI GWGCWC 3 cut(s) 26, 429, 884
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 4 cut(s) 38, 441, 639, 674
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 3 cut(s) 38, 653, 789
BsmAI GTCTC 1 cut(s) 771
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 5 cut(s) 98, 369, 488, 646, 866
Bso31I GGTCTC 1 cut(s) 771
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 3 cut(s) 26, 429, 884
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 4 cut(s) 16, 434, 925, 946
Bsp68I TCGCGA 1 cut(s) 951
BspACI CCGC 5 cut(s) 315, 458, 537, 725, 863
BspANI GGCC 5 cut(s) 98, 369, 488, 646, 866
BspCNI CTCAG 4 cut(s) 151, 154, 730, 1000
BspFNI CGCG 1 cut(s) 951
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 436, 558
BspPI GGATC 2 cut(s) 429, 442
BspTNI GGTCTC 1 cut(s) 771
BsrDI GCAATG 4 cut(s) 427, 533, 664, 712
BsrGI TGTACA 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 650
BssMI GATC 4 cut(s) 16, 434, 925, 946
BssT1I CCWWGG 1 cut(s) 650
Bst4CI ACNGT 2 cut(s) 622, 1045
Bst6I CTCTTC 1 cut(s) 900
BstAPI GCANNNNNTGC 1 cut(s) 523
BstAUI TGTACA 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 880
BstDEI CTNAG 6 cut(s) 141, 159, 717, 757, 801, 1008
BstF5I GGATG 5 cut(s) 54, 157, 558, 620, 817
BstFNI CGCG 1 cut(s) 951
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 4 cut(s) 19, 437, 928, 949
BstMAI GTCTC 1 cut(s) 771
BstMBI GATC 4 cut(s) 16, 434, 925, 946
BstMCI CGRYCG 1 cut(s) 949
BstMWI GCNNNNNNNGC 4 cut(s) 116, 494, 523, 948
BstNSI RCATGY 1 cut(s) 212
BstSCI CCNGG 3 cut(s) 37, 440, 638
BstSLI GKGCMC 1 cut(s) 26
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 6 cut(s) 429, 526, 667, 929, 978, 1009
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 5 cut(s) 98, 369, 488, 646, 866
BtgZI GCGATG 1 cut(s) 966
BtsCI GGATG 5 cut(s) 54, 157, 558, 620, 817
BtsIMutI CAGTG 2 cut(s) 26, 516
BtuMI TCGCGA 1 cut(s) 951
Cac8I GCNNGC 1 cut(s) 880
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 121
Csp6I GTAC 2 cut(s) 271, 346
CviAII CATG 5 cut(s) 100, 209, 265, 274, 383
CviQI GTAC 2 cut(s) 271, 346
DdeI CTNAG 6 cut(s) 141, 159, 717, 757, 801, 1008
DpnI GATC 4 cut(s) 18, 436, 927, 948
DpnII GATC 4 cut(s) 16, 434, 925, 946
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 900
EarI CTCTTC 1 cut(s) 900
EciI GGCGGA 1 cut(s) 473
Eco130I CCWWGG 1 cut(s) 650
Eco147I AGGCCT 2 cut(s) 488, 646
Eco31I GGTCTC 1 cut(s) 771
Eco32I GATATC 2 cut(s) 601, 1005
Eco47III AGCGCT 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 188
EcoRI GAATTC 1 cut(s) 199
EcoRV GATATC 2 cut(s) 601, 1005
EcoT14I CCWWGG 1 cut(s) 650
ErhI CCWWGG 1 cut(s) 650
FaeI CATG 5 cut(s) 103, 212, 268, 277, 386
FaiI YATR 8 cut(s) 81, 101, 197, 210, 224, 266, 275, 384
FaqI GGGAC 1 cut(s) 553
FatI CATG 5 cut(s) 99, 208, 264, 273, 382
FauI CCCGC 3 cut(s) 308, 530, 732
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 8 cut(s) 6, 418, 515, 681, 864, 943, 967, 998
FokI GGATG 5 cut(s) 61, 164, 565, 627, 804
Fsp4HI GCNGC 8 cut(s) 6, 418, 515, 681, 864, 943, 967, 998
FspBI CTAG 2 cut(s) 147, 584
GlaI GCGC 1 cut(s) 120
GluI GCNGC 8 cut(s) 6, 418, 515, 681, 864, 943, 967, 998
GsaI CCCAGC 1 cut(s) 966
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 5 cut(s) 98, 369, 488, 646, 866
HapII CCGG 4 cut(s) 38, 441, 639, 674
HhaI GCGC 1 cut(s) 121
Hin1II CATG 5 cut(s) 103, 212, 268, 277, 386
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 7 cut(s) 10, 184, 358, 541, 670, 1012, 1022
HpaII CCGG 4 cut(s) 38, 441, 639, 674
HphI GGTGA 4 cut(s) 53, 251, 634, 864
Hpy166II GTNNAC 2 cut(s) 24, 348
Hpy188I TCNGA 6 cut(s) 295, 598, 739, 916, 925, 1009
Hpy8I GTNNAC 2 cut(s) 24, 348
HpyAV CCTTC 5 cut(s) 46, 236, 572, 737, 987
HpyCH4III ACNGT 2 cut(s) 622, 1045
HpyCH4V TGCA 9 cut(s) 24, 252, 304, 400, 417, 514, 526, 618, 844
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 494, 523, 948
HpyF3I CTNAG 6 cut(s) 141, 159, 717, 757, 801, 1008
Hsp92II CATG 5 cut(s) 103, 212, 268, 277, 386
HspAI GCGC 1 cut(s) 119
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 4 cut(s) 16, 434, 925, 946
LmnI GCTCC 3 cut(s) 116, 155, 562
Lsp1109I GCAGC 6 cut(s) 429, 526, 667, 929, 978, 1009
LweI GCATC 4 cut(s) 291, 605, 755, 831
MaeI CTAG 2 cut(s) 147, 584
MaeIII GTNAC 1 cut(s) 404
MalI GATC 4 cut(s) 18, 436, 927, 948
MboI GATC 4 cut(s) 16, 434, 925, 946
MboII GAAGA 5 cut(s) 341, 353, 371, 703, 917
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 3 cut(s) 26, 429, 884
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 3 cut(s) 199, 845, 1033
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 2 cut(s) 535, 1031
MmeI TCCRAC 2 cut(s) 303, 729
Mox20I TGGCCA 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 336
MscI TGGCCA 1 cut(s) 98
MseI TTAA 2 cut(s) 180, 216
MslI CAYNNNNRTG 3 cut(s) 257, 387, 613
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 4 cut(s) 38, 441, 639, 674
MspR9I CCNGG 3 cut(s) 39, 442, 640
MvnI CGCG 1 cut(s) 951
MwoI GCNNNNNNNGC 4 cut(s) 116, 494, 523, 948
NciI CCSGG 3 cut(s) 39, 442, 640
NdeII GATC 4 cut(s) 16, 434, 925, 946
NlaIII CATG 5 cut(s) 103, 212, 268, 277, 386
NlaIV GGNNCC 2 cut(s) 436, 558
NmuCI GTSAC 1 cut(s) 404
NruI TCGCGA 1 cut(s) 951
NspI RCATGY 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 257
PaeR7I CTCGAG 1 cut(s) 188
PagI TCATGA 1 cut(s) 382
PceI AGGCCT 2 cut(s) 488, 646
PciI ACATGT 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 5 cut(s) 10, 184, 358, 670, 1012
PfoI TCCNGGA 1 cut(s) 440
PkrI GCNGC 8 cut(s) 7, 419, 516, 682, 865, 944, 968, 999
Ple19I CGATCG 1 cut(s) 949
PleI GAGTC 2 cut(s) 535, 1030
PpsI GAGTC 2 cut(s) 535, 1030
PscI ACATGT 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 962
PspN4I GGNNCC 2 cut(s) 436, 558
PsuI RGATCY 1 cut(s) 434
PvuI CGATCG 1 cut(s) 949
RgaI GCGATCGC 1 cut(s) 949
RruI TCGCGA 1 cut(s) 951
RsaI GTAC 2 cut(s) 272, 347
RsaNI GTAC 2 cut(s) 271, 346
RseI CAYNNNNRTG 3 cut(s) 257, 387, 613
SaqAI TTAA 2 cut(s) 180, 216
SatI GCNGC 8 cut(s) 6, 418, 515, 681, 864, 943, 967, 998
Sau3AI GATC 4 cut(s) 16, 434, 925, 946
SchI GAGTC 2 cut(s) 535, 1031
ScrFI CCNGG 3 cut(s) 39, 442, 640
SduI GDGCHC 3 cut(s) 26, 429, 884
SetI ASST 7 cut(s) 160, 180, 411, 735, 794, 944, 1052
SfaAI GCGATCGC 1 cut(s) 949
SfaNI GCATC 4 cut(s) 291, 605, 755, 831
Sfr274I CTCGAG 1 cut(s) 188
SgfI GCGATCGC 1 cut(s) 949
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 3 cut(s) 257, 387, 613
SmlI CTYRAG 4 cut(s) 167, 188, 371, 410
SmoI CTYRAG 4 cut(s) 167, 188, 371, 410
Sse9I AATT 3 cut(s) 199, 845, 1033
SseBI AGGCCT 2 cut(s) 488, 646
SsiI CCGC 5 cut(s) 315, 458, 537, 725, 863
SspMI CTAG 2 cut(s) 147, 584
StuI AGGCCT 2 cut(s) 488, 646
StyD4I CCNGG 3 cut(s) 37, 440, 638
StyI CCWWGG 1 cut(s) 650
TaaI ACNGT 2 cut(s) 622, 1045
TaqI TCGA 2 cut(s) 189, 839
TasI AATT 3 cut(s) 199, 845, 1033
TatI WGTACW 2 cut(s) 270, 345
TauI GCSGC 1 cut(s) 866
TfiI GAWTC 5 cut(s) 10, 184, 358, 670, 1012
Tru1I TTAA 2 cut(s) 180, 216
Tru9I TTAA 2 cut(s) 180, 216
TscAI CASTG 2 cut(s) 26, 516
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 7 cut(s) 5, 417, 514, 680, 942, 966, 997
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 7 cut(s) 65, 80, 116, 212, 253, 371, 399
TspRI CASTG 2 cut(s) 26, 516
VneI GTGCAC 1 cut(s) 22
XapI RAATTY 1 cut(s) 199
XceI RCATGY 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 2 cut(s) 147, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.