MD15G1440500.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016861
Physical Location & Seq
Forward (+)
54037639 .. 54038691
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1440500.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCGGCGAATCGCAATCAGTGCACATCTCCTCTACCAGGTGAGTGGATGAAGGGCAAACTGGTTGGGTCTGGTTCTTATGGCAGTGTTCATTTGGCCATGAACAAAACCACAGGAGCGCTTTTTGTTGTGAAATCTGCGCAGTCAAGGGATGGAGTTGATGCTCTCGAGAAAGAGGTTAAGATTCTCGAGAGTCTGAATTCCCCATACGTTGTTAAATGTATGGGGAGGGAAAATGGTGAAGGGAAATGCATCAATGTGTTCATGGAGTATATGGCAGGGGGTAGTTTGTCAGATGTGTCACAGATATTTGGCGGCGCATTGGAGGAAGAGGTGGTTCGGTTGTACACCAGAGAAATTGTTCACGGCCTCAAGTATCTTCATGAAAGTGGGATTGTCCATTGTGACCTCAAGGGCAAGAACGTGCTCTTGGGATCCTCCGGGAATGTCAAGTTGGCGGATTTTGGTTTTGCGAAAAGGCTAAAGGACTCAAAGGTTGACGGGGCTTCGGTGCAGCATTCTTTGCAATGTATTGGCGGAACTCCATTGTGGATGGCTCCTGAAGTTTTGAGAAACGAAGAGCTAGATTTTGCTTCGGATATCTGGTCCTTGGGATGCACGGTGATTGAAATGGCTACCGGGAGGCCTCCTTGGGGGAATGAGATTTGGAATCCAGTTGCTGCTGTTTTGAAGATTGCATGTGGCAACGAGAAGCCTCATTTCCCGCAAAAGCTCTCGGATGAAGGATTGGATTTCTTATCAAGGTGCTTGGAGAGAGACCCCAAAAGGAGGTGGTCTGCTAAGGAGTTGCTCAACCATCCATTTGTGTCCGGAAACTCAATGCGAGTTTCAAGAAAGGGCGCAGCCTACTCACTGGCAAGCACATTAGACATTAGTCTTTATGAAGAGGCTTCTGACTGTTCAGATGAGGGATTGAAGATCCATGATCGCGATGAGTTCCTCAGCAGCTCGAGTAGACATCCTTTTTCAAGAAGGCAGCATGTATCTGAGAATCACTTGGAGTCGTCAGGAAATTGGATTACTGTTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

351

Amino Acids

38.53

Weight (kDa)

6.43

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 18 - 276 2e-64 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 20 - 272 2.4e-39 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 88 - 165 2.1e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016861)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g28150
malus_domestica MD08G1245500.v1.1 MD15G1440500.v1.1
prunus_persica Prupe.1G582600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220281
rosa_laevigata RLG00000002243
rosa_multiflora Rmu_sc0001136.1_g000029
rosa_roxburghii Rroxscaffold_3G00239650
rosa_rugosa Rorug07G0191800
rosa_samantha Rh7AG333800 Rh7BG323300 Rh7CG351400 Rh7DG330100
rosa_wichuraiana Rw7G028160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 141
AccI GTMKAC 1 cut(s) 976
AccII CGCG 1 cut(s) 951
AccIII TCCGGA 1 cut(s) 830
AciI CCGC 5 cut(s) 5, 315, 458, 537, 725
AclWI GGATC 3 cut(s) 429, 442, 934
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 582
AfaI GTAC 1 cut(s) 347
AfeI AGCGCT 1 cut(s) 120
AfiI CCNNNNNNNGG 3 cut(s) 38, 653, 789
AgsI TTSAA 5 cut(s) 629, 691, 852, 937, 990
AjnI CCWGG 1 cut(s) 37
AjuI GAANNNNNNNTTGG 4 cut(s) 413, 437, 445, 469
AluBI AGCT 3 cut(s) 583, 733, 969
AluI AGCT 3 cut(s) 583, 733, 969
Alw21I GWGCWC 2 cut(s) 26, 429
Alw26I GTCTC 1 cut(s) 771
Alw44I GTGCAC 1 cut(s) 22
AlwI GGATC 3 cut(s) 429, 442, 934
AlwNI CAGNNNCTG 1 cut(s) 680
Ama87I CYCGRG 3 cut(s) 167, 188, 970
Aor13HI TCCGGA 1 cut(s) 830
Aor51HI AGCGCT 1 cut(s) 120
AoxI GGCC 3 cut(s) 96, 367, 644
ApaLI GTGCAC 1 cut(s) 22
ApeKI GCWGC 5 cut(s) 514, 680, 863, 966, 997
ApoI RAATTY 1 cut(s) 199
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 4 cut(s) 121, 142, 320, 863
AspS9I GGNCC 1 cut(s) 606
AsuC2I CCSGG 2 cut(s) 442, 640
AsuHPI GGTGA 3 cut(s) 53, 251, 634
AvaI CYCGRG 3 cut(s) 167, 188, 970
AvaII GGWCC 1 cut(s) 606
BaeGI GKGCMC 1 cut(s) 26
BalI TGGCCA 1 cut(s) 98
BamHI GGATCC 1 cut(s) 434
Bbv12I GWGCWC 2 cut(s) 26, 429
BbvCI CCTCAGC 1 cut(s) 962
BbvI GCAGC 5 cut(s) 526, 667, 875, 978, 1009
BccI CCATC 3 cut(s) 146, 547, 825
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 1 cut(s) 39
BcnI CCSGG 2 cut(s) 442, 640
BcoDI GTCTC 1 cut(s) 771
BfaI CTAG 1 cut(s) 584
BfoI RGCGCY 1 cut(s) 122
BisI GCNGC 7 cut(s) 6, 316, 515, 681, 864, 967, 998
BlsI GCNGC 7 cut(s) 7, 317, 516, 682, 865, 968, 999
Bme1390I CCNGG 3 cut(s) 39, 442, 640
Bme18I GGWCC 1 cut(s) 606
BmeT110I CYCGRG 3 cut(s) 167, 188, 970
BmgT120I GGNCC 1 cut(s) 606
BmiI GGNNCC 2 cut(s) 436, 558
BmrFI CCNGG 3 cut(s) 39, 442, 640
BmsI GCATC 3 cut(s) 151, 261, 605
BoxI GACNNNNGTC 1 cut(s) 894
Bpu10I CCTNAGC 2 cut(s) 801, 962
BpuEI CTTGAG 2 cut(s) 356, 395
BpuMI CCSGG 2 cut(s) 442, 640
BsaI GGTCTC 1 cut(s) 771
BsaJI CCNNGG 2 cut(s) 609, 650
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 653, 789
Bse1I ACTGG 3 cut(s) 66, 674, 879
Bse3DI GCAATG 1 cut(s) 533
BseAI TCCGGA 1 cut(s) 830
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 2 cut(s) 609, 650
BseGI GGATG 7 cut(s) 54, 157, 558, 620, 745, 817, 979
BseLI CCNNNNNNNGG 3 cut(s) 38, 653, 789
BseMI GCAATG 1 cut(s) 533
BseMII CTCAG 2 cut(s) 976, 999
BseNI ACTGG 3 cut(s) 66, 674, 879
BseRI GAGGAG 1 cut(s) 21
BseSI GKGCMC 1 cut(s) 26
BseXI GCAGC 5 cut(s) 526, 667, 875, 978, 1009
BsgI GTGCAG 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 951
BshFI GGCC 3 cut(s) 98, 369, 646
BsiHKAI GWGCWC 2 cut(s) 26, 429
BsiHKCI CYCGRG 3 cut(s) 167, 188, 970
BsiSI CCGG 3 cut(s) 441, 639, 831
BslI CCNNNNNNNGG 3 cut(s) 38, 653, 789
BsmAI GTCTC 1 cut(s) 771
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 3 cut(s) 98, 369, 646
Bso31I GGTCTC 1 cut(s) 771
BsoBI CYCGRG 3 cut(s) 167, 188, 970
Bsp1286I GDGCHC 2 cut(s) 26, 429
Bsp13I TCCGGA 1 cut(s) 830
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 3 cut(s) 434, 939, 946
Bsp68I TCGCGA 1 cut(s) 951
BspACI CCGC 5 cut(s) 5, 315, 458, 537, 725
BspANI GGCC 3 cut(s) 98, 369, 646
BspCNI CTCAG 2 cut(s) 975, 1000
BspEI TCCGGA 1 cut(s) 830
BspFNI CGCG 1 cut(s) 951
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 436, 558
BspPI GGATC 3 cut(s) 429, 442, 934
BspQI GCTCTTC 1 cut(s) 573
BspTNI GGTCTC 1 cut(s) 771
BsrDI GCAATG 1 cut(s) 533
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 3 cut(s) 66, 674, 879
BssECI CCNNGG 2 cut(s) 609, 650
BssMI GATC 3 cut(s) 434, 939, 946
BssT1I CCWWGG 2 cut(s) 609, 650
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 3 cut(s) 622, 920, 1045
Bst6I CTCTTC 3 cut(s) 324, 573, 900
BstAPI GCANNNNNTGC 2 cut(s) 21, 523
BstAUI TGTACA 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 880
BstDEI CTNAG 3 cut(s) 801, 962, 1008
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 7 cut(s) 54, 157, 558, 620, 745, 817, 979
BstFNI CGCG 1 cut(s) 951
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 4 cut(s) 121, 142, 320, 863
BstKTI GATC 3 cut(s) 437, 942, 949
BstMAI GTCTC 1 cut(s) 771
BstMBI GATC 3 cut(s) 434, 939, 946
BstMWI GCNNNNNNNGC 2 cut(s) 21, 523
BstNI CCWGG 1 cut(s) 39
BstNSI RCATGY 2 cut(s) 702, 1004
BstPAI GACNNNNGTC 1 cut(s) 894
BstSCI CCNGG 3 cut(s) 37, 440, 638
BstSLI GKGCMC 1 cut(s) 26
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 5 cut(s) 526, 667, 875, 978, 1009
BstX2I RGATCY 2 cut(s) 434, 939
BstXI CCANNNNNNTGG 1 cut(s) 45
BstYI RGATCY 2 cut(s) 434, 939
BsuRI GGCC 3 cut(s) 98, 369, 646
BtgZI GCGATG 1 cut(s) 966
BtsCI GGATG 7 cut(s) 54, 157, 558, 620, 745, 817, 979
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 3 cut(s) 26, 91, 872
BtuMI TCGCGA 1 cut(s) 951
Cac8I GCNNGC 1 cut(s) 880
CaiI CAGNNNCTG 1 cut(s) 680
CciI TCATGA 1 cut(s) 382
CfoI GCGC 4 cut(s) 121, 142, 320, 863
Cfr13I GGNCC 1 cut(s) 606
CsiI ACCWGGT 1 cut(s) 37
Csp6I GTAC 1 cut(s) 346
CviAII CATG 6 cut(s) 100, 265, 383, 699, 944, 1001
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 3 cut(s) 801, 962, 1008
DpnI GATC 3 cut(s) 436, 941, 948
DpnII GATC 3 cut(s) 434, 939, 946
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 3 cut(s) 324, 573, 900
EarI CTCTTC 3 cut(s) 324, 573, 900
EciI GGCGGA 2 cut(s) 473, 552
Eco130I CCWWGG 2 cut(s) 609, 650
Eco147I AGGCCT 1 cut(s) 646
Eco31I GGTCTC 1 cut(s) 771
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 1 cut(s) 606
Eco47III AGCGCT 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 582
Eco88I CYCGRG 3 cut(s) 167, 188, 970
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 37
EcoRV GATATC 1 cut(s) 601
EcoT14I CCWWGG 2 cut(s) 609, 650
EcoT22I ATGCAT 1 cut(s) 254
ErhI CCWWGG 2 cut(s) 609, 650
FaeI CATG 6 cut(s) 103, 268, 386, 702, 947, 1004
FatI CATG 6 cut(s) 99, 264, 382, 698, 943, 1000
FauI CCCGC 1 cut(s) 732
FblI GTMKAC 1 cut(s) 976
Fnu4HI GCNGC 7 cut(s) 6, 316, 515, 681, 864, 967, 998
FokI GGATG 7 cut(s) 61, 164, 565, 627, 752, 804, 966
Fsp4HI GCNGC 7 cut(s) 6, 316, 515, 681, 864, 967, 998
FspBI CTAG 1 cut(s) 584
FspI TGCGCA 1 cut(s) 141
GlaI GCGC 4 cut(s) 120, 141, 319, 862
GluI GCNGC 7 cut(s) 6, 316, 515, 681, 864, 967, 998
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 3 cut(s) 98, 369, 646
HapII CCGG 3 cut(s) 441, 639, 831
HhaI GCGC 4 cut(s) 121, 142, 320, 863
Hin1II CATG 6 cut(s) 103, 268, 386, 702, 947, 1004
Hin6I GCGC 4 cut(s) 119, 140, 318, 861
HinP1I GCGC 4 cut(s) 119, 140, 318, 861
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 7 cut(s) 10, 184, 193, 488, 670, 1012, 1022
HpaII CCGG 3 cut(s) 441, 639, 831
HphI GGTGA 3 cut(s) 53, 251, 634
Hpy166II GTNNAC 5 cut(s) 24, 348, 364, 499, 977
Hpy188I TCNGA 7 cut(s) 198, 295, 598, 739, 916, 925, 1009
Hpy8I GTNNAC 5 cut(s) 24, 348, 364, 499, 977
HpyAV CCTTC 4 cut(s) 46, 236, 737, 987
HpyCH4III ACNGT 3 cut(s) 622, 920, 1045
HpyCH4IV ACGT 2 cut(s) 210, 423
HpyCH4V TGCA 6 cut(s) 24, 252, 514, 526, 618, 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 523
HpyF3I CTNAG 3 cut(s) 801, 962, 1008
HpySE526I ACGT 2 cut(s) 210, 423
Hsp92II CATG 6 cut(s) 103, 268, 386, 702, 947, 1004
HspAI GCGC 4 cut(s) 119, 140, 318, 861
Kpn2I TCCGGA 1 cut(s) 830
Kzo9I GATC 3 cut(s) 434, 939, 946
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 116, 562
Lsp1109I GCAGC 5 cut(s) 526, 667, 875, 978, 1009
LweI GCATC 3 cut(s) 151, 261, 605
MabI ACCWGGT 1 cut(s) 37
MaeI CTAG 1 cut(s) 584
MaeII ACGT 2 cut(s) 210, 423
MaeIII GTNAC 2 cut(s) 300, 404
MalI GATC 3 cut(s) 436, 941, 948
MboI GATC 3 cut(s) 434, 939, 946
MboII GAAGA 6 cut(s) 341, 371, 590, 703, 917, 949
MflI RGATCY 2 cut(s) 434, 939
MhlI GDGCHC 2 cut(s) 26, 429
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 3 cut(s) 199, 357, 1033
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 3 cut(s) 202, 482, 1031
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 1 cut(s) 254
MroI TCCGGA 1 cut(s) 830
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 98
MseI TTAA 2 cut(s) 180, 216
MslI CAYNNNNRTG 2 cut(s) 257, 387
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 3 cut(s) 441, 639, 831
MspR9I CCNGG 3 cut(s) 39, 442, 640
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 1 cut(s) 39
MvnI CGCG 1 cut(s) 951
MwoI GCNNNNNNNGC 2 cut(s) 21, 523
NciI CCSGG 2 cut(s) 442, 640
NdeII GATC 3 cut(s) 434, 939, 946
NlaIII CATG 6 cut(s) 103, 268, 386, 702, 947, 1004
NlaIV GGNNCC 2 cut(s) 436, 558
NmuCI GTSAC 2 cut(s) 300, 404
NruI TCGCGA 1 cut(s) 951
NsbI TGCGCA 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 2 cut(s) 702, 1004
PaeR7I CTCGAG 3 cut(s) 167, 188, 970
PagI TCATGA 1 cut(s) 382
PceI AGGCCT 1 cut(s) 646
PciSI GCTCTTC 1 cut(s) 573
PctI GAATGC 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 4 cut(s) 10, 184, 670, 1012
PfoI TCCNGGA 1 cut(s) 440
PkrI GCNGC 7 cut(s) 7, 317, 516, 682, 865, 968, 999
PleI GAGTC 3 cut(s) 201, 482, 1030
PpsI GAGTC 3 cut(s) 201, 482, 1030
PshAI GACNNNNGTC 1 cut(s) 894
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 436, 558
PspPI GGNCC 1 cut(s) 606
PspXI VCTCGAGB 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 2 cut(s) 434, 939
RruI TCGCGA 1 cut(s) 951
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 2 cut(s) 257, 387
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 180, 216
SatI GCNGC 7 cut(s) 6, 316, 515, 681, 864, 967, 998
Sau3AI GATC 3 cut(s) 434, 939, 946
Sau96I GGNCC 1 cut(s) 606
SchI GAGTC 3 cut(s) 202, 482, 1031
ScrFI CCNGG 3 cut(s) 39, 442, 640
SduI GDGCHC 2 cut(s) 26, 429
SexAI ACCWGGT 1 cut(s) 37
SfaNI GCATC 3 cut(s) 151, 261, 605
Sfr274I CTCGAG 3 cut(s) 167, 188, 970
SinI GGWCC 1 cut(s) 606
SlaI CTCGAG 3 cut(s) 167, 188, 970
SmiMI CAYNNNNRTG 2 cut(s) 257, 387
SmlI CTYRAG 5 cut(s) 167, 188, 371, 410, 970
SmoI CTYRAG 5 cut(s) 167, 188, 371, 410, 970
Sse9I AATT 3 cut(s) 199, 357, 1033
SseBI AGGCCT 1 cut(s) 646
SsiI CCGC 5 cut(s) 5, 315, 458, 537, 725
SspMI CTAG 1 cut(s) 584
StuI AGGCCT 1 cut(s) 646
StyD4I CCNGG 3 cut(s) 37, 440, 638
StyI CCWWGG 2 cut(s) 609, 650
TaaI ACNGT 3 cut(s) 622, 920, 1045
TaiI ACGT 2 cut(s) 213, 426
TaqI TCGA 3 cut(s) 168, 189, 971
TasI AATT 3 cut(s) 199, 357, 1033
TatI WGTACW 1 cut(s) 345
TauI GCSGC 2 cut(s) 8, 318
TfiI GAWTC 4 cut(s) 10, 184, 670, 1012
Tru1I TTAA 2 cut(s) 180, 216
Tru9I TTAA 2 cut(s) 180, 216
TscAI CASTG 3 cut(s) 26, 91, 879
TseFI GTSAC 2 cut(s) 300, 404
TseI GCWGC 5 cut(s) 514, 680, 863, 966, 997
Tsp45I GTSAC 2 cut(s) 300, 404
TspDTI ATGAA 8 cut(s) 65, 80, 116, 253, 371, 399, 756, 918
TspRI CASTG 3 cut(s) 26, 91, 879
VneI GTGCAC 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 606
XagI CCTNNNNNAGG 1 cut(s) 36
XapI RAATTY 1 cut(s) 199
XceI RCATGY 2 cut(s) 702, 1004
XhoI CTCGAG 3 cut(s) 167, 188, 970
XmiI GTMKAC 1 cut(s) 976
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 584
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.