Prupe.1G582600_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016861
Physical Location & Seq
Forward (+)
47324368 .. 47326091
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G582600.1

Sequence Viewer

Length: 1083 bp
ATGGCTTCAAATTCTGATCAATGCACATCTCCTCTGCCTGGTGCATGGATGAAGGGCAAACTGGTTGGATCTGGTGCTTTTGGCAGTGTCCATTTGGCCATGAGCAAAGCCACAGGAGCACTTTTTGTTGTGAAATCTGCAGTGTCTGGGCATGGAGTTCAGGCTCTGGAGAATGAGGCAAAGATTCTCGAGAGTTTGAATTCGCAATACGTTGTTCGATGCCTGGGGAAGGAAAATGGTGAAGGGAAATGCATCAATGTGTTCATGGAGTACATGGCAGGGGGTAGCTTGTCAGATGTGTCACAGATATTTGGTGGTGCATTGGAGGAAGAGGTGGTTCGTTTGTACACCAGAGAGATTCTTCTTGGTCTCAAGTATCTTCATGAAAATGGGATTGTGCATTGTGACCTCAAGTGCAAGAATGTGCTCGTGGGCTCCTCCGGGAATGTGAAGCTGGCGGATTTTGGCTCTGCTAAGAGGCTAAAGGACTCAAAGGCTCATGGGGTTTCCGTGCAGGTTGCTGTGCAATCTATTGGTGGGACTCCATTGTGGATGGCTCCTGAAGTTTTGAGAAATGAAGGGCTGGATTTCGCTTTGGATATTTGGTCATTAGGATGCACTGTCATTGAAATGGCTACTGGCAGGCCTCCTTGGGGTACCAAGGCTTCGAATCCGGTGGCTGCTGTTTTGGAGATTGCTTGTGGCAATGAGAAGCCTCAGTTTCCAAGACATTTCTCAGATAATGGGTTGGATTTCTTAGCAAGGTGCTTGGAGAGAGACCCGAAAAGGAGGTGGTCTGCTGAGGAATTGCTTAACCATCCATTTGTATCAGGAAATTCAATGAGAGTTTCAAGAAAGGGTGTGGCTTGCTCACCTGCAAGTACTCTAGACATTGGAATTTGTGAGGAGGGCTCTGATTACTCATCAGATCATGGAATGGGGATTCGCGATCGCGATGATTTCCCGAGCATCCCGAGCAGAAATCCTTTCTCAAAGTGCTGTGATGAAGGAAACAGAAGCACAAGAACGCAGCAGACTGAGAGTCACTTGGAGTCATCAGAAAATTGGATCACTGTTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

361

Amino Acids

38.91

Weight (kDa)

6.17

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016861)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g28150
malus_domestica MD08G1245500.v1.1 MD15G1440500.v1.1
prunus_persica Prupe.1G582600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220281
rosa_laevigata RLG00000002243
rosa_multiflora Rmu_sc0001136.1_g000029
rosa_roxburghii Rroxscaffold_3G00239650
rosa_rugosa Rorug07G0191800
rosa_samantha Rh7AG333800 Rh7BG323300 Rh7CG351400 Rh7DG330100
rosa_wichuraiana Rw7G028160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 883
Acc36I ACCTGC 2 cut(s) 505, 883
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccII CGCG 2 cut(s) 948, 954
AciI CCGC 1 cut(s) 458
AclWI GGATC 2 cut(s) 76, 1076
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 4 cut(s) 10, 199, 835, 897
AcuI CTGAAG 1 cut(s) 582
AfaI GTAC 4 cut(s) 272, 347, 658, 883
AfiI CCNNNNNNNGG 3 cut(s) 38, 229, 653
AgsI TTSAA 5 cut(s) 9, 199, 629, 840, 852
AhdI GACNNNNNGTC 1 cut(s) 1041
AjnI CCWGG 2 cut(s) 37, 222
AluBI AGCT 2 cut(s) 288, 454
AluI AGCT 2 cut(s) 288, 454
Alw21I GWGCWC 2 cut(s) 121, 429
Alw26I GTCTC 2 cut(s) 374, 771
AlwI GGATC 2 cut(s) 76, 1076
AlwNI CAGNNNCTG 2 cut(s) 146, 166
Ama87I CYCGRG 3 cut(s) 188, 964, 973
AoxI GGCC 2 cut(s) 96, 644
ApeKI GCWGC 2 cut(s) 680, 1030
ApoI RAATTY 4 cut(s) 10, 199, 835, 897
AsiSI GCGATCGC 1 cut(s) 952
Asp718I GGTACC 1 cut(s) 656
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 251, 864
AsuII TTCGAA 1 cut(s) 668
AvaI CYCGRG 3 cut(s) 188, 964, 973
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 2 cut(s) 437, 914
BarI GAAGNNNNNNTAC 2 cut(s) 649, 681
BauI CACGAG 1 cut(s) 428
Bbv12I GWGCWC 2 cut(s) 121, 429
BbvCI CCTCAGC 1 cut(s) 801
BbvI GCAGC 2 cut(s) 667, 1042
BccI CCATC 2 cut(s) 547, 825
BciT130I CCWGG 2 cut(s) 39, 224
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 2 cut(s) 374, 771
BfaI CTAG 1 cut(s) 887
BfmI CTRYAG 1 cut(s) 138
BfuAI ACCTGC 2 cut(s) 505, 883
BisI GCNGC 2 cut(s) 681, 1031
BlsI GCNGC 2 cut(s) 682, 1032
BmcAI AGTACT 1 cut(s) 883
Bme1390I CCNGG 3 cut(s) 39, 224, 442
BmeRI GACNNNNNGTC 1 cut(s) 1041
BmeT110I CYCGRG 3 cut(s) 188, 964, 973
BmiI GGNNCC 3 cut(s) 436, 558, 658
BmrFI CCNGG 3 cut(s) 39, 224, 442
BmsI GCATC 4 cut(s) 209, 261, 605, 978
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 2 cut(s) 356, 395
BpuMI CCSGG 1 cut(s) 442
BsaI GGTCTC 2 cut(s) 374, 771
BsaJI CCNNGG 3 cut(s) 223, 650, 660
BsaWI WCCGGW 1 cut(s) 673
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 229, 653
Bse1I ACTGG 2 cut(s) 66, 643
Bse3DI GCAATG 1 cut(s) 712
BseBI CCWGG 2 cut(s) 39, 224
BseDI CCNNGG 3 cut(s) 223, 650, 660
BseGI GGATG 5 cut(s) 54, 558, 620, 817, 969
BseLI CCNNNNNNNGG 3 cut(s) 38, 229, 653
BseMI GCAATG 1 cut(s) 712
BseMII CTCAG 4 cut(s) 731, 750, 792, 1029
BseNI ACTGG 2 cut(s) 66, 643
BseRI GAGGAG 3 cut(s) 21, 427, 920
BseXI GCAGC 2 cut(s) 667, 1042
BsgI GTGCAG 1 cut(s) 533
Bsh1236I CGCG 2 cut(s) 948, 954
Bsh1285I CGRYCG 1 cut(s) 952
BshFI GGCC 2 cut(s) 98, 646
BshNI GGYRCC 1 cut(s) 656
BsiEI CGRYCG 1 cut(s) 952
BsiHKAI GWGCWC 2 cut(s) 121, 429
BsiHKCI CYCGRG 3 cut(s) 188, 964, 973
BsiSI CCGG 2 cut(s) 441, 674
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 3 cut(s) 38, 229, 653
BsmAI GTCTC 2 cut(s) 374, 771
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 2 cut(s) 98, 646
Bso31I GGTCTC 2 cut(s) 374, 771
BsoBI CYCGRG 3 cut(s) 188, 964, 973
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 4 cut(s) 121, 429, 437, 914
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 5 cut(s) 16, 68, 928, 949, 1068
Bsp68I TCGCGA 2 cut(s) 948, 954
BspACI CCGC 1 cut(s) 458
BspANI GGCC 2 cut(s) 98, 646
BspCNI CTCAG 4 cut(s) 730, 749, 793, 1030
BspFNI CGCG 2 cut(s) 948, 954
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 436, 558, 658
BspMAI CTGCAG 1 cut(s) 142
BspMI ACCTGC 2 cut(s) 505, 883
BspPI GGATC 2 cut(s) 76, 1076
BspT104I TTCGAA 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 656
BspTNI GGTCTC 2 cut(s) 374, 771
BsrDI GCAATG 1 cut(s) 712
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 2 cut(s) 66, 643
BssECI CCNNGG 3 cut(s) 223, 650, 660
BssMI GATC 5 cut(s) 16, 68, 928, 949, 1068
BssSI CACGAG 1 cut(s) 428
BssT1I CCWWGG 2 cut(s) 650, 660
Bst2BI CACGAG 1 cut(s) 428
Bst2UI CCWGG 2 cut(s) 39, 224
Bst4CI ACNGT 2 cut(s) 622, 1075
Bst6I CTCTTC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 345
BstBI TTCGAA 1 cut(s) 668
BstC8I GCNNGC 3 cut(s) 456, 644, 868
BstDEI CTNAG 6 cut(s) 474, 717, 736, 757, 801, 1038
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 5 cut(s) 54, 558, 620, 817, 969
BstFNI CGCG 2 cut(s) 948, 954
BstKTI GATC 5 cut(s) 19, 71, 931, 952, 1071
BstMAI GTCTC 2 cut(s) 374, 771
BstMBI GATC 5 cut(s) 16, 68, 928, 949, 1068
BstMCI CGRYCG 1 cut(s) 952
BstMWI GCNNNNNNNGC 2 cut(s) 116, 975
BstNI CCWGG 2 cut(s) 39, 224
BstSCI CCNGG 3 cut(s) 37, 222, 440
BstSFI CTRYAG 1 cut(s) 138
BstUI CGCG 2 cut(s) 948, 954
BstV1I GCAGC 2 cut(s) 667, 1042
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 2 cut(s) 98, 646
BtgZI GCGATG 1 cut(s) 969
BtsCI GGATG 5 cut(s) 54, 558, 620, 817, 969
BtsI GCAGTG 2 cut(s) 91, 147
BtsIMutI CAGTG 4 cut(s) 91, 147, 618, 1071
BtuMI TCGCGA 2 cut(s) 948, 954
BveI ACCTGC 2 cut(s) 505, 883
Cac8I GCNNGC 3 cut(s) 456, 644, 868
CaiI CAGNNNCTG 2 cut(s) 146, 166
CciI TCATGA 1 cut(s) 382
Csp6I GTAC 4 cut(s) 271, 346, 657, 882
CviAII CATG 8 cut(s) 45, 100, 152, 265, 274, 383, 500, 932
CviQI GTAC 4 cut(s) 271, 346, 657, 882
DdeI CTNAG 6 cut(s) 474, 717, 736, 757, 801, 1038
DpnI GATC 5 cut(s) 18, 70, 930, 951, 1070
DpnII GATC 5 cut(s) 16, 68, 928, 949, 1068
DriI GACNNNNNGTC 1 cut(s) 1041
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 324
Eam1105I GACNNNNNGTC 1 cut(s) 1041
EarI CTCTTC 1 cut(s) 324
EciI GGCGGA 1 cut(s) 473
Eco130I CCWWGG 2 cut(s) 650, 660
Eco147I AGGCCT 1 cut(s) 646
Eco24I GRGCYC 2 cut(s) 437, 914
Eco31I GGTCTC 2 cut(s) 374, 771
Eco57I CTGAAG 1 cut(s) 582
Eco88I CYCGRG 3 cut(s) 188, 964, 973
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 2 cut(s) 37, 222
EcoT14I CCWWGG 2 cut(s) 650, 660
EcoT22I ATGCAT 1 cut(s) 254
EcoT38I GRGCYC 2 cut(s) 437, 914
ErhI CCWWGG 2 cut(s) 650, 660
FaeI CATG 8 cut(s) 48, 103, 155, 268, 277, 386, 503, 935
FaiI YATR 8 cut(s) 46, 101, 153, 266, 275, 384, 501, 933
FaqI GGGAC 1 cut(s) 553
FatI CATG 8 cut(s) 44, 99, 151, 264, 273, 382, 499, 931
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 681, 1031
FokI GGATG 5 cut(s) 61, 565, 627, 804, 956
FriOI GRGCYC 2 cut(s) 437, 914
Fsp4HI GCNGC 2 cut(s) 681, 1031
FspBI CTAG 1 cut(s) 887
GluI GCNGC 2 cut(s) 681, 1031
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 2 cut(s) 98, 646
HapII CCGG 2 cut(s) 441, 674
Hin1II CATG 8 cut(s) 48, 103, 155, 268, 277, 386, 503, 935
HinfI GANTC 8 cut(s) 184, 358, 488, 541, 670, 943, 1042, 1052
HpaII CCGG 2 cut(s) 441, 674
HphI GGTGA 2 cut(s) 251, 864
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 6 cut(s) 16, 295, 739, 916, 928, 1060
Hpy8I GTNNAC 1 cut(s) 348
HpyAV CCTTC 5 cut(s) 46, 223, 236, 572, 1001
HpyCH4III ACNGT 2 cut(s) 622, 1075
HpyCH4IV ACGT 1 cut(s) 210
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 975
HpyF3I CTNAG 6 cut(s) 474, 717, 736, 757, 801, 1038
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 8 cut(s) 48, 103, 155, 268, 277, 386, 503, 935
KpnI GGTACC 1 cut(s) 660
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 68, 928, 949, 1068
LmnI GCTCC 3 cut(s) 116, 440, 562
Lsp1109I GCAGC 2 cut(s) 667, 1042
LweI GCATC 4 cut(s) 209, 261, 605, 978
MaeI CTAG 1 cut(s) 887
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 3 cut(s) 300, 404, 1043
MalI GATC 5 cut(s) 18, 70, 930, 951, 1070
MboI GATC 5 cut(s) 16, 68, 928, 949, 1068
MboII GAAGA 3 cut(s) 341, 353, 371
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 4 cut(s) 121, 429, 437, 914
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 6 cut(s) 10, 199, 806, 835, 897, 1063
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 4 cut(s) 482, 535, 1051, 1061
MmeI TCCRAC 2 cut(s) 46, 729
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 1 cut(s) 254
MscI TGGCCA 1 cut(s) 98
MseI TTAA 1 cut(s) 813
MslI CAYNNNNRTG 4 cut(s) 257, 387, 613, 629
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 2 cut(s) 441, 674
MspR9I CCNGG 3 cut(s) 39, 224, 442
MvaI CCWGG 2 cut(s) 39, 224
MvnI CGCG 2 cut(s) 948, 954
MwoI GCNNNNNNNGC 2 cut(s) 116, 975
NciI CCSGG 1 cut(s) 442
NdeII GATC 5 cut(s) 16, 68, 928, 949, 1068
NlaIII CATG 8 cut(s) 48, 103, 155, 268, 277, 386, 503, 935
NlaIV GGNNCC 3 cut(s) 436, 558, 658
NmuCI GTSAC 3 cut(s) 300, 404, 1043
NruI TCGCGA 2 cut(s) 948, 954
NsiI ATGCAT 1 cut(s) 254
NspV TTCGAA 1 cut(s) 668
PaeR7I CTCGAG 1 cut(s) 188
PagI TCATGA 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 883
PceI AGGCCT 1 cut(s) 646
PfeI GAWTC 4 cut(s) 184, 358, 670, 943
PfoI TCCNGGA 1 cut(s) 440
PkrI GCNGC 2 cut(s) 682, 1032
Ple19I CGATCG 1 cut(s) 952
PleI GAGTC 4 cut(s) 482, 535, 1050, 1060
PpsI GAGTC 4 cut(s) 482, 535, 1050, 1060
Psp6I CCWGG 2 cut(s) 37, 222
PspGI CCWGG 2 cut(s) 37, 222
PspN4I GGNNCC 3 cut(s) 436, 558, 658
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 2 cut(s) 146, 166
PsuI RGATCY 1 cut(s) 68
PvuI CGATCG 1 cut(s) 952
RgaI GCGATCGC 1 cut(s) 952
RruI TCGCGA 2 cut(s) 948, 954
RsaI GTAC 4 cut(s) 272, 347, 658, 883
RsaNI GTAC 4 cut(s) 271, 346, 657, 882
RseI CAYNNNNRTG 4 cut(s) 257, 387, 613, 629
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 2 cut(s) 681, 1031
Sau3AI GATC 5 cut(s) 16, 68, 928, 949, 1068
ScaI AGTACT 1 cut(s) 883
SchI GAGTC 4 cut(s) 482, 535, 1051, 1061
ScrFI CCNGG 3 cut(s) 39, 224, 442
SduI GDGCHC 4 cut(s) 121, 429, 437, 914
SfaAI GCGATCGC 1 cut(s) 952
SfaNI GCATC 4 cut(s) 209, 261, 605, 978
SfcI CTRYAG 1 cut(s) 138
Sfr274I CTCGAG 1 cut(s) 188
SfuI TTCGAA 1 cut(s) 668
SgfI GCGATCGC 1 cut(s) 952
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 4 cut(s) 257, 387, 613, 629
SmlI CTYRAG 3 cut(s) 188, 371, 410
SmoI CTYRAG 3 cut(s) 188, 371, 410
Sse9I AATT 6 cut(s) 10, 199, 806, 835, 897, 1063
SseBI AGGCCT 1 cut(s) 646
SsiI CCGC 1 cut(s) 458
SspMI CTAG 1 cut(s) 887
StuI AGGCCT 1 cut(s) 646
StyD4I CCNGG 3 cut(s) 37, 222, 440
StyI CCWWGG 2 cut(s) 650, 660
TaaI ACNGT 2 cut(s) 622, 1075
TaiI ACGT 1 cut(s) 213
TaqI TCGA 3 cut(s) 189, 217, 668
TasI AATT 6 cut(s) 10, 199, 806, 835, 897, 1063
TatI WGTACW 3 cut(s) 270, 345, 881
TfiI GAWTC 4 cut(s) 184, 358, 670, 943
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 4 cut(s) 91, 147, 625, 1078
TseFI GTSAC 3 cut(s) 300, 404, 1043
TseI GCWGC 2 cut(s) 680, 1030
Tsp45I GTSAC 3 cut(s) 300, 404, 1043
TspDTI ATGAA 6 cut(s) 65, 253, 371, 399, 591, 1020
TspGWI ACGGA 1 cut(s) 499
TspRI CASTG 4 cut(s) 91, 147, 625, 1078
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 4 cut(s) 10, 199, 835, 897
XbaI TCTAGA 1 cut(s) 886
XhoI CTCGAG 1 cut(s) 188
XspI CTAG 1 cut(s) 887
ZrmI AGTACT 1 cut(s) 883
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.