Rroxscaffold_3G00239650

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016861
Physical Location & Seq
Forward (+)
30115161 .. 30117080
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239650.1

Sequence Viewer

Length: 1068 bp
ATGGGTAGAATTGATGAATGCACATCTCCTCTGCCTGGTGAATGGATGAAGGGCAAATTGGTTGGATCCGGTTCTTTTGGAAGTGTCCATTTGGCCATGAGCAAATCCACAGGAGGACTTTTCGTCGTGAAGTCGTCGCAGTCTCGGGATGGAGCGCTCGCTCTTGAGAATGAGGCTAAGATTCTCGACAGTTTGAATTCCCCATATGTTGTTCGATGCATGGGGATCGAAAATGGTAAAGAGAAATTGTGCAATGTGTTTATGGAGTACATGGCAGGGGGTAGTTTGTCAGATGTGGCTCAGATATTTGGCGGGGCTTTCGATGAAGAAGTGGTTCGTTTGTACTCGAGAGAGATTCTTCATGGTCTCAAGTATCTTCACGAAAATGGGATCGTGCATTGTGATCTCAAGTGCAAGAATGTGCTCTTGAGCTCCTCCGGGAACGTGAAGCTGGCAGACTTTGGTTGTGCTAAGAGGCTCAAGGACTCAAACACCAAGAATGGGGGTAGCTCCACGCCTTCCACCACTGTTGGTGGAACTCCATTGTGGATGGCTCCTGAAGTTTTGAGGAATGAAGGGCTGGATTTTGCTTGTGACATTTGGTCATTGGGATGCACCGTGATTGAAATGGCCACCGGAAGGCCTCCTTGGGCTGCTGAGATATCTGATCCGAGAGCCGCTGTTTTGAAGATTGCTTGCAGCAACGAGAGGCCTCAGTTTCCGAGACAGTTTTCAAGTGAGGGGTCGGATTTCTTAGCGAAATGCTTGGAAAGAGACCCGAAAAGGAGGTGGTCTGCTGAGCAACTACTTAACCATCCCTTTGTCTCTGGAAATCCAATGAGAGTTTCAAGAAAGGGCGAGACATGCTCACCGGCAAGTACTTTGGACGATATTGGAACTTACGATGAGAGTTCTGATTGTAGTTCTGATCGTGGAATGGAGAGCTGTGAGGGGAACGAGTTGACCAGCAGAAATCCTTTCTCGAGGTGTTTTGATGAAAGAAACAGAAGTACAGTAAGGCCTGAGTGTCACTTTGAGTCATCTGAAAATTGGATCACTGTTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

355

Amino Acids

38.88

Weight (kDa)

5.67

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 17 - 275 3.1e-66 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 19 - 271 1.7e-40 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 87 - 164 1.3e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016861)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g28150
malus_domestica MD08G1245500.v1.1 MD15G1440500.v1.1
prunus_persica Prupe.1G582600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220281
rosa_laevigata RLG00000002243
rosa_multiflora Rmu_sc0001136.1_g000029
rosa_roxburghii Rroxscaffold_3G00239650
rosa_rugosa Rorug07G0191800
rosa_samantha Rh7AG333800 Rh7BG323300 Rh7CG351400 Rh7DG330100
rosa_wichuraiana Rw7G028160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 312, 678
AclWI GGATC 6 cut(s) 60, 73, 233, 398, 662, 1061
AcoI YGGCCR 2 cut(s) 93, 630
AcsI RAATTY 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 4 cut(s) 269, 344, 880, 1012
AfeI AGCGCT 1 cut(s) 156
AfiI CCNNNNNNNGG 3 cut(s) 35, 501, 639
AgsI TTSAA 5 cut(s) 196, 626, 688, 735, 849
AhdI GACNNNNNGTC 2 cut(s) 122, 601
AjnI CCWGG 1 cut(s) 34
AjuI GAANNNNNNNTTGG 6 cut(s) 41, 73, 631, 663, 829, 861
AluBI AGCT 4 cut(s) 432, 451, 510, 945
AluI AGCT 4 cut(s) 432, 451, 510, 945
Alw21I GWGCWC 2 cut(s) 426, 434
Alw26I GTCTC 6 cut(s) 147, 371, 718, 768, 829, 854
AlwI GGATC 6 cut(s) 60, 73, 233, 398, 662, 1061
Ama87I CYCGRG 3 cut(s) 144, 346, 982
Aor51HI AGCGCT 1 cut(s) 156
AoxI GGCC 5 cut(s) 93, 630, 641, 710, 1019
ApeKI GCWGC 2 cut(s) 653, 699
ApoI RAATTY 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 50, 861
AvaI CYCGRG 3 cut(s) 144, 346, 982
BalI TGGCCA 2 cut(s) 95, 632
BamHI GGATCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 434
Bbv12I GWGCWC 2 cut(s) 426, 434
BbvI GCAGC 2 cut(s) 640, 711
BccI CCATC 3 cut(s) 143, 544, 822
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 1 cut(s) 36
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 6 cut(s) 147, 371, 718, 768, 829, 854
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 3 cut(s) 654, 678, 700
BlpI GCTNAGC 1 cut(s) 798
BlsI GCNGC 3 cut(s) 655, 679, 701
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 2 cut(s) 36, 439
BmeRI GACNNNNNGTC 2 cut(s) 122, 601
BmeT110I CYCGRG 3 cut(s) 144, 346, 982
BmiI GGNNCC 2 cut(s) 67, 555
BmrFI CCNGG 2 cut(s) 36, 439
BmsI GCATC 2 cut(s) 206, 602
BplI GAGNNNNNCTC 2 cut(s) 851, 883
Bpu1102I GCTNAGC 1 cut(s) 798
BpuEI CTTGAG 5 cut(s) 185, 353, 392, 448, 464
BpuMI CCSGG 1 cut(s) 439
BsaI GGTCTC 2 cut(s) 371, 768
BsaJI CCNNGG 1 cut(s) 647
BsaWI WCCGGW 2 cut(s) 68, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 501, 639
Bse118I RCCGGY 1 cut(s) 871
Bse3DI GCAATG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 647
BseGI GGATG 5 cut(s) 51, 154, 555, 617, 814
BseLI CCNNNNNNNGG 3 cut(s) 35, 501, 639
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 5 cut(s) 314, 648, 728, 789, 1014
BseRI GAGGAG 2 cut(s) 18, 424
BseXI GCAGC 2 cut(s) 640, 711
BshFI GGCC 5 cut(s) 95, 632, 643, 712, 1021
BsiHKAI GWGCWC 2 cut(s) 426, 434
BsiHKCI CYCGRG 3 cut(s) 144, 346, 982
BsiSI CCGG 4 cut(s) 69, 438, 636, 872
BslI CCNNNNNNNGG 3 cut(s) 35, 501, 639
BsmAI GTCTC 6 cut(s) 147, 371, 718, 768, 829, 854
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 5 cut(s) 95, 632, 643, 712, 1021
Bso31I GGTCTC 2 cut(s) 371, 768
BsoBI CYCGRG 3 cut(s) 144, 346, 982
Bsp1286I GDGCHC 2 cut(s) 426, 434
Bsp143I GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
Bsp1720I GCTNAGC 1 cut(s) 798
BspACI CCGC 2 cut(s) 312, 678
BspANI GGCC 5 cut(s) 95, 632, 643, 712, 1021
BspCNI CTCAG 5 cut(s) 313, 649, 727, 790, 1015
BspLI GGNNCC 2 cut(s) 67, 555
BspPI GGATC 6 cut(s) 60, 73, 233, 398, 662, 1061
BspTNI GGTCTC 2 cut(s) 371, 768
BsrDI GCAATG 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 871
BssAI RCCGGY 1 cut(s) 871
BssECI CCNNGG 1 cut(s) 647
BssMI GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
BssT1I CCWWGG 1 cut(s) 647
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 6 cut(s) 191, 529, 619, 729, 1015, 1060
BstC8I GCNNGC 3 cut(s) 159, 453, 697
BstDEI CTNAG 8 cut(s) 177, 300, 471, 657, 714, 754, 798, 1023
BstF5I GGATG 5 cut(s) 51, 154, 555, 617, 814
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 7 cut(s) 68, 228, 393, 406, 670, 931, 1056
BstMAI GTCTC 6 cut(s) 147, 371, 718, 768, 829, 854
BstMBI GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
BstMWI GCNNNNNNNGC 1 cut(s) 864
BstNI CCWGG 1 cut(s) 36
BstNSI RCATGY 1 cut(s) 867
BstSCI CCNGG 2 cut(s) 34, 437
BstV1I GCAGC 2 cut(s) 640, 711
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 5 cut(s) 95, 632, 643, 712, 1021
BtsCI GGATG 5 cut(s) 51, 154, 555, 617, 814
BtsIMutI CAGTG 2 cut(s) 525, 1056
Cac8I GCNNGC 3 cut(s) 159, 453, 697
CfoI GCGC 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 871
Csp6I GTAC 4 cut(s) 268, 343, 879, 1011
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviAII CATG 5 cut(s) 97, 220, 271, 362, 864
CviQI GTAC 4 cut(s) 268, 343, 879, 1011
DdeI CTNAG 8 cut(s) 177, 300, 471, 657, 714, 754, 798, 1023
DpnI GATC 7 cut(s) 67, 227, 392, 405, 669, 930, 1055
DpnII GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
DriI GACNNNNNGTC 2 cut(s) 122, 601
EaeI YGGCCR 2 cut(s) 93, 630
Eam1105I GACNNNNNGTC 2 cut(s) 122, 601
Ecl136II GAGCTC 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 3 cut(s) 643, 712, 1021
Eco24I GRGCYC 1 cut(s) 434
Eco31I GGTCTC 2 cut(s) 371, 768
Eco32I GATATC 1 cut(s) 663
Eco47III AGCGCT 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 432
Eco57I CTGAAG 1 cut(s) 579
Eco88I CYCGRG 3 cut(s) 144, 346, 982
EcoICRI GAGCTC 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 34
EcoRV GATATC 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 647
EcoT22I ATGCAT 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 5 cut(s) 100, 223, 274, 365, 867
FaiI YATR 8 cut(s) 98, 205, 207, 221, 263, 272, 363, 865
FalI AAGNNNNNCTT 2 cut(s) 631, 663
FatI CATG 5 cut(s) 96, 219, 270, 361, 863
FauI CCCGC 1 cut(s) 305
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 654, 678, 700
FokI GGATG 5 cut(s) 58, 161, 562, 624, 801
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 3 cut(s) 654, 678, 700
GlaI GCGC 1 cut(s) 156
GluI GCNGC 3 cut(s) 654, 678, 700
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 5 cut(s) 95, 632, 643, 712, 1021
HapII CCGG 4 cut(s) 69, 438, 636, 872
HhaI GCGC 1 cut(s) 157
Hin1II CATG 5 cut(s) 100, 223, 274, 365, 867
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 963
HindII GTYRAC 1 cut(s) 963
HinfI GANTC 4 cut(s) 181, 355, 485, 1037
HpaII CCGG 4 cut(s) 69, 438, 636, 872
HphI GGTGA 2 cut(s) 50, 861
Hpy166II GTNNAC 1 cut(s) 963
Hpy188I TCNGA 9 cut(s) 292, 303, 667, 672, 723, 748, 916, 928, 1045
Hpy8I GTNNAC 1 cut(s) 963
Hpy99I CGWCG 2 cut(s) 128, 139
HpyAV CCTTC 4 cut(s) 43, 528, 569, 633
HpyCH4III ACNGT 6 cut(s) 191, 529, 619, 729, 1015, 1060
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 7 cut(s) 21, 219, 252, 397, 414, 615, 699
HpyF10VI GCNNNNNNNGC 1 cut(s) 864
HpyF3I CTNAG 8 cut(s) 177, 300, 471, 657, 714, 754, 798, 1023
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 5 cut(s) 100, 223, 274, 365, 867
HspAI GCGC 1 cut(s) 155
Kzo9I GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
LmnI GCTCC 4 cut(s) 152, 437, 515, 559
Lsp1109I GCAGC 2 cut(s) 640, 711
LweI GCATC 2 cut(s) 206, 602
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 593, 1028
MalI GATC 7 cut(s) 67, 227, 392, 405, 669, 930, 1055
MboI GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
MboII GAAGA 4 cut(s) 338, 350, 368, 700
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 2 cut(s) 426, 434
MlsI TGGCCA 2 cut(s) 95, 632
MluCI AATT 5 cut(s) 9, 56, 196, 245, 1048
MluNI TGGCCA 2 cut(s) 95, 632
MlyI GAGTC 2 cut(s) 479, 1046
MmeI TCCRAC 2 cut(s) 43, 726
Mox20I TGGCCA 2 cut(s) 95, 632
Mph1103I ATGCAT 1 cut(s) 221
MroXI GAANNNNTTC 1 cut(s) 333
MscI TGGCCA 2 cut(s) 95, 632
MseI TTAA 1 cut(s) 810
MslI CAYNNNNRTG 2 cut(s) 384, 610
Msp20I TGGCCA 2 cut(s) 95, 632
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 4 cut(s) 69, 438, 636, 872
MspR9I CCNGG 2 cut(s) 36, 439
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 1 cut(s) 36
MwoI GCNNNNNNNGC 1 cut(s) 864
NciI CCSGG 1 cut(s) 439
NdeI CATATG 1 cut(s) 205
NdeII GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
NlaIII CATG 5 cut(s) 100, 223, 274, 365, 867
NlaIV GGNNCC 2 cut(s) 67, 555
NmuCI GTSAC 2 cut(s) 593, 1028
NsiI ATGCAT 1 cut(s) 221
NspI RCATGY 1 cut(s) 867
PaeR7I CTCGAG 2 cut(s) 346, 982
PceI AGGCCT 3 cut(s) 643, 712, 1021
PctI GAATGC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 181, 355
PfoI TCCNGGA 1 cut(s) 437
PkrI GCNGC 3 cut(s) 655, 679, 701
PleI GAGTC 2 cut(s) 479, 1045
PpsI GAGTC 2 cut(s) 479, 1045
Psp124BI GAGCTC 1 cut(s) 434
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 67, 555
PsuI RGATCY 1 cut(s) 65
RsaI GTAC 4 cut(s) 269, 344, 880, 1012
RsaNI GTAC 4 cut(s) 268, 343, 879, 1011
RseI CAYNNNNRTG 2 cut(s) 384, 610
SacI GAGCTC 1 cut(s) 434
SaqAI TTAA 1 cut(s) 810
SatI GCNGC 3 cut(s) 654, 678, 700
Sau3AI GATC 7 cut(s) 65, 225, 390, 403, 667, 928, 1053
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 2 cut(s) 479, 1046
ScrFI CCNGG 2 cut(s) 36, 439
SduI GDGCHC 2 cut(s) 426, 434
SetI ASST 7 cut(s) 434, 447, 453, 512, 791, 947, 989
SfaNI GCATC 2 cut(s) 206, 602
Sfr274I CTCGAG 2 cut(s) 346, 982
SlaI CTCGAG 2 cut(s) 346, 982
SmiMI CAYNNNNRTG 2 cut(s) 384, 610
SmlI CTYRAG 7 cut(s) 164, 346, 368, 407, 427, 479, 982
SmoI CTYRAG 7 cut(s) 164, 346, 368, 407, 427, 479, 982
Sse9I AATT 5 cut(s) 9, 56, 196, 245, 1048
SseBI AGGCCT 3 cut(s) 643, 712, 1021
SsiI CCGC 2 cut(s) 312, 678
SstI GAGCTC 1 cut(s) 434
StuI AGGCCT 3 cut(s) 643, 712, 1021
StyD4I CCNGG 2 cut(s) 34, 437
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 6 cut(s) 191, 529, 619, 729, 1015, 1060
TaiI ACGT 1 cut(s) 447
TaqI TCGA 6 cut(s) 186, 214, 228, 321, 347, 983
TasI AATT 5 cut(s) 9, 56, 196, 245, 1048
TatI WGTACW 4 cut(s) 267, 342, 878, 1010
TauI GCSGC 1 cut(s) 680
TfiI GAWTC 2 cut(s) 181, 355
Tru1I TTAA 1 cut(s) 810
Tru9I TTAA 1 cut(s) 810
TscAI CASTG 2 cut(s) 532, 1063
TseFI GTSAC 2 cut(s) 593, 1028
TseI GCWGC 2 cut(s) 653, 699
Tsp45I GTSAC 2 cut(s) 593, 1028
TspDTI ATGAA 6 cut(s) 30, 62, 339, 350, 588, 1011
TspRI CASTG 2 cut(s) 532, 1063
XapI RAATTY 1 cut(s) 196
XceI RCATGY 1 cut(s) 867
XhoI CTCGAG 2 cut(s) 346, 982
XmnI GAANNNNTTC 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 880
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.