FvH4_5g32900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016047
Physical Location & Seq
Reverse (-)
23752873 .. 23754851
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32900.t1

Sequence Viewer

Length: 1110 bp
ATGAGGAAGTTTAAAGAGTTGGGGAGGTTGTTATTTGCACTAGTGGTTTGGCTGCGGTTGAGTGCCAGGGCTTGCTCGCAGCCACAGCAGCAGCAAGTCCCTTGCTTCTTCATTTTCGGTGACTCCTTGGTGGACAATGGAAACAACAATCGACTGCTCACACTTTCTAGAGCAAATTATAGGCCTTATGGCATCGACTTTCCTCAGGGTGTCACCGGCCGCTTCACCAATGGTCGAACCTATGTTGATGTACTAGCTCAGCTTCTGGGGTTTCCAACTTTCATTCCGCCTTATTCAAGAACTCGAGGAGCTGCACTTATGAGGGGAGTGAATCTTGCATCGGGAGCATCTGGCATTCGAGATGAAACTGGAGATAATTTGGGTGGTCACACATCGATGAACCAACAAGTCCAGACTTTTGGAAATGAAATACTTCCGGAAATGAGGAGGGTGTTAAGAGGAGACGCCAATGCACTAAGCAGCTATCTTAGCAGATGCATCTTCTATTCTGGGATGGGGAGTAATGATTATCTTAACAATTATTACATGCCTGACTTCTACTCGACCAGTTCCGATTTCACTACAAAAGCTTATGCTGATGCACTTCTAAAAGACTACTCGCGCCAGCTCACTCAACTGTATTCCTTTGGAGCTAGAAAAGTGATTGTGACAGCAATTGGCCAAATTGGCTGCATACCCTATGAACTAGCAAGGTTTCGTGGAAACAACAGCCGGTGCAATGAAGACATCAACAAAGCTATTGTTATGTTCAACTCAGGGCTTAAAAAACTTGTTGACAGTTTCAATGGCGATCAACTGCCTGGAGCCAAGTTTGTCTACGTAGATGCTTATCAAAGCAACGTTGACCTCTATCAAAACCCAAAGGCTTCTGGATTTGATGTGATTGATAAGGGGTGTTGTGGAGTAGGAAGGAACAATGGGCAGCTAACTTGTCTTCCTCTTCAAGAAGCTTGTCGAGATCGAACGAAGTACCTGTTTTGGGATGCCTTCCATCCAACAGATACAGCAAACGTTGGGTTCGGCAAGGTAACATTCAGTTCCAGACAGTATACGTATCCCATCAATATACAACAATTGGCAATGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

41.28

Weight (kDa)

9.12

Isoelectric Point (pI)

28.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 344 1.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33811
fragaria_vesca FvH4_5g32900
malus_domestica MD08G1215700.v1.1
prunus_persica Prupe.1G547400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0230871
rosa_laevigata RLG00000001456
rosa_multiflora Rmu_sc0000551.1_g000017 Rmu_sc0010407.1_g000024
rosa_roxburghii Rroxscaffold_3G00229730
rosa_rugosa Rorug07G0262700
rosa_samantha Rh7BG392000 Rh7CG434900 Rh7DG410400
rosa_wichuraiana Rw7G034290 Rw7G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 837, 1070
AccII CGCG 1 cut(s) 622
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 3 cut(s) 55, 220, 287
AclI AACGTT 2 cut(s) 861, 1032
AcoI YGGCCR 2 cut(s) 217, 679
AcyI GRCGYC 1 cut(s) 465
AfaI GTAC 2 cut(s) 252, 992
AfiI CCNNNNNNNGG 1 cut(s) 1000
AgsI TTSAA 4 cut(s) 297, 772, 805, 965
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 2 cut(s) 65, 820
Alw26I GTCTC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 3 cut(s) 182, 217, 679
ApeKI GCWGC 8 cut(s) 52, 79, 88, 91, 311, 480, 690, 943
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 624
AsuHPI GGTGA 3 cut(s) 131, 205, 217
AvaI CYCGRG 1 cut(s) 303
AxyI CCTNAGG 1 cut(s) 204
BalI TGGCCA 1 cut(s) 681
BbsI GAAGAC 2 cut(s) 750, 947
BbvI GCAGC 8 cut(s) 39, 91, 100, 103, 298, 492, 677, 955
BccI CCATC 3 cut(s) 508, 1020, 1088
BciT130I CCWGG 2 cut(s) 67, 822
BciVI GTATCC 1 cut(s) 1086
BcoDI GTCTC 1 cut(s) 456
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 5 cut(s) 41, 168, 254, 654, 707
BfuI GTATCC 1 cut(s) 1086
BglI GCCNNNNNGGC 1 cut(s) 687
BisI GCNGC 9 cut(s) 53, 80, 89, 92, 220, 312, 481, 691, 944
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 9 cut(s) 54, 81, 90, 93, 221, 313, 482, 692, 945
Bme1390I CCNGG 2 cut(s) 67, 822
BmeT110I CYCGRG 1 cut(s) 303
BmiI GGNNCC 1 cut(s) 826
BmrFI CCNGG 2 cut(s) 67, 822
BmsI GCATC 8 cut(s) 201, 347, 356, 485, 507, 589, 835, 994
BpiI GAAGAC 2 cut(s) 750, 947
BpmI CTGGAG 2 cut(s) 390, 843
Bpu1102I GCTNAGC 1 cut(s) 258
Bsa29I ATCGAT 1 cut(s) 395
BsaAI YACGTR 2 cut(s) 841, 1074
BsaBI GATNNNNATC 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 66, 126
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 1000
Bse118I RCCGGY 2 cut(s) 215, 732
Bse1I ACTGG 2 cut(s) 373, 567
Bse21I CCTNAGG 1 cut(s) 204
Bse3DI GCAATG 2 cut(s) 745, 1107
Bse8I GATNNNNATC 1 cut(s) 849
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 2 cut(s) 67, 822
BseCI ATCGAT 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 66, 126
BseGI GGATG 3 cut(s) 519, 1009, 1012
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 1 cut(s) 1000
BseMI GCAATG 2 cut(s) 745, 1107
BseMII CTCAG 3 cut(s) 218, 272, 789
BseNI ACTGG 2 cut(s) 373, 567
BseRI GAGGAG 3 cut(s) 321, 460, 474
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 8 cut(s) 39, 91, 100, 103, 298, 492, 677, 955
BsgI GTGCAG 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 622
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 3 cut(s) 184, 219, 681
BshVI ATCGAT 1 cut(s) 395
BsiEI CGRYCG 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 3 cut(s) 216, 437, 733
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 1000
BsmAI GTCTC 1 cut(s) 456
BsmBI CGTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 83
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 3 cut(s) 184, 219, 681
BsoBI CYCGRG 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 2 cut(s) 811, 979
Bsp1720I GCTNAGC 1 cut(s) 258
BspACI CCGC 3 cut(s) 55, 220, 287
BspANI GGCC 3 cut(s) 184, 219, 681
BspCNI CTCAG 3 cut(s) 217, 271, 788
BspDI ATCGAT 1 cut(s) 395
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 1 cut(s) 622
BspLI GGNNCC 1 cut(s) 826
BsrDI GCAATG 2 cut(s) 745, 1107
BsrFI RCCGGY 2 cut(s) 215, 732
BsrI ACTGG 2 cut(s) 373, 567
BssAI RCCGGY 2 cut(s) 215, 732
BssECI CCNNGG 2 cut(s) 66, 126
BssMI GATC 2 cut(s) 811, 979
BssNAI GTATAC 1 cut(s) 1071
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 1 cut(s) 126
Bst1107I GTATAC 1 cut(s) 1071
Bst2UI CCWGG 2 cut(s) 67, 822
Bst4CI ACNGT 3 cut(s) 639, 800, 1068
Bst6I CTCTTC 1 cut(s) 966
BstACI GRCGYC 1 cut(s) 465
BstBAI YACGTR 2 cut(s) 841, 1074
BstC8I GCNNGC 3 cut(s) 73, 77, 626
BstDEI CTNAG 5 cut(s) 204, 258, 476, 488, 775
BstF5I GGATG 3 cut(s) 519, 1009, 1012
BstFNI CGCG 1 cut(s) 622
BstHHI GCGC 1 cut(s) 624
BstKTI GATC 2 cut(s) 814, 982
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 2 cut(s) 811, 979
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 5 cut(s) 85, 88, 344, 489, 687
BstNI CCWGG 2 cut(s) 67, 822
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 2 cut(s) 65, 820
BstSNI TACGTA 2 cut(s) 841, 1074
BstUI CGCG 1 cut(s) 622
BstV1I GCAGC 8 cut(s) 39, 91, 100, 103, 298, 492, 677, 955
BstV2I GAAGAC 2 cut(s) 750, 947
BstXI CCANNNNNNTGG 1 cut(s) 419
BstZ17I GTATAC 1 cut(s) 1071
BstZI CGGCCG 1 cut(s) 217
Bsu15I ATCGAT 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 204
BsuI GTATCC 1 cut(s) 1086
BsuRI GGCC 3 cut(s) 184, 219, 681
BsuTUI ATCGAT 1 cut(s) 395
BtsCI GGATG 3 cut(s) 519, 1009, 1012
Cac8I GCNNGC 3 cut(s) 73, 77, 626
CaiI CAGNNNCTG 1 cut(s) 265
CfoI GCGC 1 cut(s) 624
Cfr10I RCCGGY 2 cut(s) 215, 732
ClaI ATCGAT 1 cut(s) 395
CseI GACGC 1 cut(s) 473
Csp6I GTAC 2 cut(s) 251, 991
CviAII CATG 1 cut(s) 547
CviQI GTAC 2 cut(s) 251, 991
DdeI CTNAG 5 cut(s) 204, 258, 476, 488, 775
DpnI GATC 2 cut(s) 813, 981
DpnII GATC 2 cut(s) 811, 979
DraI TTTAAA 1 cut(s) 13
EaeI YGGCCR 2 cut(s) 217, 679
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
EciI GGCGGA 1 cut(s) 276
EclXI CGGCCG 1 cut(s) 217
Eco105I TACGTA 2 cut(s) 841, 1074
Eco130I CCWWGG 1 cut(s) 126
Eco147I AGGCCT 1 cut(s) 184
Eco52I CGGCCG 1 cut(s) 217
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 65, 820
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 500
ErhI CCWWGG 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 456
FaeI CATG 1 cut(s) 550
FaqI GGGAC 1 cut(s) 83
FatI CATG 1 cut(s) 546
FblI GTMKAC 2 cut(s) 837, 1070
Fnu4HI GCNGC 9 cut(s) 53, 80, 89, 92, 220, 312, 481, 691, 944
FokI GGATG 3 cut(s) 526, 999, 1016
Fsp4HI GCNGC 9 cut(s) 53, 80, 89, 92, 220, 312, 481, 691, 944
FspBI CTAG 5 cut(s) 41, 168, 254, 654, 707
GlaI GCGC 1 cut(s) 623
GluI GCNGC 9 cut(s) 53, 80, 89, 92, 220, 312, 481, 691, 944
GsuI CTGGAG 2 cut(s) 390, 843
HaeIII GGCC 3 cut(s) 184, 219, 681
HapII CCGG 3 cut(s) 216, 437, 733
HgaI GACGC 1 cut(s) 473
HhaI GCGC 1 cut(s) 624
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 1 cut(s) 550
Hin6I GCGC 1 cut(s) 622
HinP1I GCGC 1 cut(s) 622
HincII GTYRAC 2 cut(s) 796, 865
HindII GTYRAC 2 cut(s) 796, 865
HindIII AAGCTT 2 cut(s) 588, 969
HinfI GANTC 2 cut(s) 122, 331
HpaII CCGG 3 cut(s) 216, 437, 733
HphI GGTGA 3 cut(s) 131, 205, 217
Hpy166II GTNNAC 5 cut(s) 133, 796, 838, 865, 1071
Hpy188I TCNGA 1 cut(s) 574
Hpy8I GTNNAC 5 cut(s) 133, 796, 838, 865, 1071
HpyAV CCTTC 2 cut(s) 924, 1018
HpyCH4III ACNGT 3 cut(s) 639, 800, 1068
HpyCH4IV ACGT 4 cut(s) 840, 861, 1032, 1073
HpyCH4V TGCA 8 cut(s) 38, 314, 338, 473, 498, 602, 693, 738
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 88, 344, 489, 687
HpyF3I CTNAG 5 cut(s) 204, 258, 476, 488, 775
HpySE526I ACGT 4 cut(s) 840, 861, 1032, 1073
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 1 cut(s) 550
HspAI GCGC 1 cut(s) 622
Kpn2I TCCGGA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 811, 979
LmnI GCTCC 4 cut(s) 308, 344, 650, 824
Lsp1109I GCAGC 8 cut(s) 39, 91, 100, 103, 298, 492, 677, 955
LweI GCATC 8 cut(s) 201, 347, 356, 485, 507, 589, 835, 994
MaeI CTAG 5 cut(s) 41, 168, 254, 654, 707
MaeII ACGT 4 cut(s) 840, 861, 1032, 1073
MaeIII GTNAC 5 cut(s) 119, 211, 386, 667, 1048
MalI GATC 2 cut(s) 813, 981
MboI GATC 2 cut(s) 811, 979
MboII GAAGA 5 cut(s) 100, 493, 755, 947, 953
MfeI CAATTG 2 cut(s) 675, 1094
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 6 cut(s) 175, 376, 538, 675, 684, 1094
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 299, 1040
MnlI CCTC 9 cut(s) 18, 213, 299, 315, 438, 441, 452, 878, 969
Mox20I TGGCCA 1 cut(s) 681
Mph1103I ATGCAT 1 cut(s) 500
MroI TCCGGA 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 1 cut(s) 681
MseI TTAA 4 cut(s) 12, 455, 534, 783
MslI CAYNNNNRTG 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 681
MspI CCGG 3 cut(s) 216, 437, 733
MspR9I CCNGG 2 cut(s) 67, 822
MunI CAATTG 2 cut(s) 675, 1094
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 2 cut(s) 67, 822
MvnI CGCG 1 cut(s) 622
MwoI GCNNNNNNNGC 5 cut(s) 85, 88, 344, 489, 687
NdeII GATC 2 cut(s) 811, 979
NlaIII CATG 1 cut(s) 550
NlaIV GGNNCC 1 cut(s) 826
NmuCI GTSAC 4 cut(s) 119, 211, 386, 667
NsiI ATGCAT 1 cut(s) 500
NspI RCATGY 1 cut(s) 550
PaeR7I CTCGAG 1 cut(s) 303
PceI AGGCCT 1 cut(s) 184
PctI GAATGC 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 9 cut(s) 54, 81, 90, 93, 221, 313, 482, 692, 945
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Ppu21I YACGTR 2 cut(s) 841, 1074
Psp1406I AACGTT 2 cut(s) 861, 1032
Psp6I CCWGG 2 cut(s) 65, 820
PspGI CCWGG 2 cut(s) 65, 820
PspN4I GGNNCC 1 cut(s) 826
PspXI VCTCGAGB 1 cut(s) 303
PstNI CAGNNNCTG 1 cut(s) 265
RsaI GTAC 2 cut(s) 252, 992
RsaNI GTAC 2 cut(s) 251, 991
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 4 cut(s) 12, 455, 534, 783
SatI GCNGC 9 cut(s) 53, 80, 89, 92, 220, 312, 481, 691, 944
Sau3AI GATC 2 cut(s) 811, 979
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 67, 822
SfaNI GCATC 8 cut(s) 201, 347, 356, 485, 507, 589, 835, 994
Sfr274I CTCGAG 1 cut(s) 303
SlaI CTCGAG 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
SnaBI TACGTA 2 cut(s) 841, 1074
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 6 cut(s) 175, 376, 538, 675, 684, 1094
SseBI AGGCCT 1 cut(s) 184
SsiI CCGC 3 cut(s) 55, 220, 287
SspMI CTAG 5 cut(s) 41, 168, 254, 654, 707
StuI AGGCCT 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 65, 820
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 3 cut(s) 639, 800, 1068
TaiI ACGT 4 cut(s) 843, 864, 1035, 1076
TaqI TCGA 9 cut(s) 151, 195, 235, 304, 358, 395, 563, 976, 982
TasI AATT 6 cut(s) 175, 376, 538, 675, 684, 1094
TatI WGTACW 1 cut(s) 250
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 4 cut(s) 12, 455, 534, 783
Tru9I TTAA 4 cut(s) 12, 455, 534, 783
TseFI GTSAC 4 cut(s) 119, 211, 386, 667
TseI GCWGC 8 cut(s) 52, 79, 88, 91, 311, 480, 690, 943
Tsp45I GTSAC 4 cut(s) 119, 211, 386, 667
TspDTI ATGAA 7 cut(s) 100, 271, 378, 413, 441, 717, 756
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 1 cut(s) 550
XhoI CTCGAG 1 cut(s) 303
XmiI GTMKAC 2 cut(s) 837, 1070
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 5 cut(s) 41, 168, 254, 654, 707
Zsp2I ATGCAT 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.