Rw7G034450

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016047
Physical Location & Seq
Reverse (-)
51122787 .. 51125229
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G034450.1

Sequence Viewer

Length: 1113 bp
ATGAGGAAGTCTAAAGAGTTGGGGAGGTTGTTATTTGTACTAGTGGTTTGGCTACGGTTGAGTGCCAGGGCTTGCTCGCAGCCACTGCAGCAGCAGCAAGTCCCTTGCTTCTTCATTTTCGGTGACTCGTTGGTGGACAATGGAAATAACAATAGAATGATCACACTTGCTAGGGCAAATTATAGGCCTTATGGCGTTGACTTTCCTCAGGGTGTCACCGGCCGCTTCACCAATGGTCGAACCTATGTCGATGTACTAGCTGAGCTTCTGGGGTTTCGAACTTATATTCCGCCTTATTCAAGAACTCGTGGAGCTGCACTTATGAGGGGAGTGAATCTTGCATCAGGGGCATCTGGCATTCGAGATGAAACTGGAGATAATTTGGGTGGTCACACATCGATGAACCAACAAGTGTTGACCTTTGGAAATAATATACTGCCAGAAATGAGAAGGTTGTTTAGAGGAGACGCCAATGAACTAAACAGCTATCTTAGCAGATGCATCTTCTATTCTGGGATGGGAAGTAATGATTATCTTAACAATTATTACATGCCAAACTTCTACACAACCAGTTCTGATTTCACTACAAAAGCTTATGCTGCTGCACTTCTTCAGGACTACTCGCGCCAGCTCACACAATTGTATTCCTTCGGAGCTCGAAAAGTGATTGTGACAGCAATTGGCCAAATTGGCTGCATACCATATGAACTAGCAAGATTTCATGGCAACAACAGCCGTTGCAATGAAGACATCAACAATGCCATTGTTATGTTCAACTCGGGGCTTAAAAAACTTGTTGACAATTTCAATGGCGGTCAATTGCCTGGAGCCAAGTTTGTCTACGTAGATGCTTATCAAAGCAACGTTGACCTCTATAAAAGCCCAAATGCTTCTGGATTTGCAGTGATTGACAAGGGGTGTTGTGGAGTAGGAAGGAACAATGGGCAGATAACTTGTCTTCCACTCCAAGAAGCTTGCCAAGATCGCACGAAGCACTTGTTTTGGGATGCCTTCCATCCAACAGATACAGCAAACGTTGGGTTCGGCAAGGCAACATTCAGTTCCGGACAGTATACGTATCCAATCAATATTCAACAATTGGCAATGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

41.17

Weight (kDa)

9.06

Isoelectric Point (pI)

28.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 345 6.8e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33811
fragaria_vesca FvH4_5g32900
malus_domestica MD08G1215700.v1.1
prunus_persica Prupe.1G547400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0230871
rosa_laevigata RLG00000001456
rosa_multiflora Rmu_sc0000551.1_g000017 Rmu_sc0010407.1_g000024
rosa_roxburghii Rroxscaffold_3G00229730
rosa_rugosa Rorug07G0262700
rosa_samantha Rh7BG392000 Rh7CG434900 Rh7DG410400
rosa_wichuraiana Rw7G034290 Rw7G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 840, 1073
AccII CGCG 1 cut(s) 625
AccIII TCCGGA 1 cut(s) 1064
AciI CCGC 3 cut(s) 223, 290, 813
AclI AACGTT 2 cut(s) 864, 1035
AcoI YGGCCR 2 cut(s) 220, 682
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 468
AfaI GTAC 2 cut(s) 39, 255
AgsI TTSAA 4 cut(s) 300, 775, 808, 1094
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 2 cut(s) 65, 823
AloI GAACNNNNNNTCC 2 cut(s) 271, 303
AluBI AGCT 8 cut(s) 260, 265, 314, 486, 593, 631, 656, 974
AluI AGCT 8 cut(s) 260, 265, 314, 486, 593, 631, 656, 974
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 778
Aor13HI TCCGGA 1 cut(s) 1064
AoxI GGCC 3 cut(s) 185, 220, 682
ApeKI GCWGC 8 cut(s) 79, 88, 91, 94, 314, 599, 602, 693
AspLEI GCGC 1 cut(s) 627
AsuHPI GGTGA 3 cut(s) 134, 208, 220
AsuII TTCGAA 1 cut(s) 277
AvaI CYCGRG 1 cut(s) 778
AxyI CCTNAGG 1 cut(s) 207
BalI TGGCCA 1 cut(s) 684
BanII GRGCYC 1 cut(s) 658
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 2 cut(s) 753, 950
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 8 cut(s) 91, 100, 103, 106, 301, 586, 589, 680
BccI CCATC 2 cut(s) 511, 1023
BceAI ACGGC 1 cut(s) 720
BciT130I CCWGG 2 cut(s) 67, 825
BciVI GTATCC 1 cut(s) 1089
BclI TGATCA 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 459
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 171, 257, 710
BfmI CTRYAG 1 cut(s) 86
BfuI GTATCC 1 cut(s) 1089
BglI GCCNNNNNGGC 1 cut(s) 690
BisI GCNGC 9 cut(s) 80, 89, 92, 95, 223, 315, 600, 603, 694
BlpI GCTNAGC 1 cut(s) 261
BlsI GCNGC 9 cut(s) 81, 90, 93, 96, 224, 316, 601, 604, 695
Bme1390I CCNGG 2 cut(s) 67, 825
BmeT110I CYCGRG 1 cut(s) 778
BmiI GGNNCC 1 cut(s) 829
BmrFI CCNGG 2 cut(s) 67, 825
BmsI GCATC 6 cut(s) 350, 359, 488, 510, 838, 997
BpiI GAAGAC 2 cut(s) 753, 950
BpmI CTGGAG 2 cut(s) 393, 846
Bpu1102I GCTNAGC 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 277
Bsa29I ATCGAT 1 cut(s) 398
BsaAI YACGTR 2 cut(s) 844, 1077
BsaBI GATNNNNATC 1 cut(s) 852
BsaHI GRCGYC 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 1064
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 376, 570
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 2 cut(s) 748, 1110
Bse8I GATNNNNATC 1 cut(s) 852
BseAI TCCGGA 1 cut(s) 1064
BseBI CCWGG 2 cut(s) 67, 825
BseCI ATCGAT 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 3 cut(s) 522, 1012, 1015
BseJI GATNNNNATC 1 cut(s) 852
BseMI GCAATG 2 cut(s) 748, 1110
BseMII CTCAG 2 cut(s) 221, 252
BseNI ACTGG 2 cut(s) 376, 570
BseRI GAGGAG 1 cut(s) 477
BseX3I CGGCCG 1 cut(s) 220
BseXI GCAGC 8 cut(s) 91, 100, 103, 106, 301, 586, 589, 680
BsgI GTGCAG 2 cut(s) 300, 588
Bsh1236I CGCG 1 cut(s) 625
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 187, 222, 684
BshVI ATCGAT 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 2 cut(s) 219, 1065
BslFI GGGAC 1 cut(s) 86
BsmAI GTCTC 1 cut(s) 459
BsmBI CGTCTC 1 cut(s) 459
BsmFI GGGAC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 3 cut(s) 187, 222, 684
BsoBI CYCGRG 1 cut(s) 778
Bsp119I TTCGAA 1 cut(s) 277
Bsp1286I GDGCHC 1 cut(s) 658
Bsp13I TCCGGA 1 cut(s) 1064
Bsp143I GATC 2 cut(s) 159, 982
Bsp1720I GCTNAGC 1 cut(s) 261
BspACI CCGC 3 cut(s) 223, 290, 813
BspANI GGCC 3 cut(s) 187, 222, 684
BspCNI CTCAG 2 cut(s) 220, 253
BspDI ATCGAT 1 cut(s) 398
BspEI TCCGGA 1 cut(s) 1064
BspFNI CGCG 1 cut(s) 625
BspLI GGNNCC 1 cut(s) 829
BspMAI CTGCAG 1 cut(s) 90
BspT104I TTCGAA 1 cut(s) 277
BsrDI GCAATG 2 cut(s) 748, 1110
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 2 cut(s) 376, 570
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 2 cut(s) 159, 982
BssNAI GTATAC 1 cut(s) 1074
BssNI GRCGYC 1 cut(s) 468
BssSI CACGAG 1 cut(s) 306
Bst1107I GTATAC 1 cut(s) 1074
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 67, 825
Bst4CI ACNGT 2 cut(s) 57, 1071
BstACI GRCGYC 1 cut(s) 468
BstAPI GCANNNNNTGC 1 cut(s) 85
BstBAI YACGTR 2 cut(s) 844, 1077
BstBI TTCGAA 1 cut(s) 277
BstC8I GCNNGC 4 cut(s) 73, 77, 629, 976
BstDEI CTNAG 3 cut(s) 207, 261, 491
BstF5I GGATG 3 cut(s) 522, 1012, 1015
BstFNI CGCG 1 cut(s) 625
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 2 cut(s) 162, 985
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 2 cut(s) 159, 982
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 9 cut(s) 85, 88, 94, 347, 492, 599, 690, 732, 984
BstNI CCWGG 2 cut(s) 67, 825
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 2 cut(s) 65, 823
BstSFI CTRYAG 1 cut(s) 86
BstSNI TACGTA 2 cut(s) 844, 1077
BstUI CGCG 1 cut(s) 625
BstV1I GCAGC 8 cut(s) 91, 100, 103, 106, 301, 586, 589, 680
BstV2I GAAGAC 2 cut(s) 753, 950
BstZ17I GTATAC 1 cut(s) 1074
BstZI CGGCCG 1 cut(s) 220
Bsu15I ATCGAT 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 207
BsuI GTATCC 1 cut(s) 1089
BsuRI GGCC 3 cut(s) 187, 222, 684
BsuTUI ATCGAT 1 cut(s) 398
BtsCI GGATG 3 cut(s) 522, 1012, 1015
BtsI GCAGTG 2 cut(s) 83, 909
BtsIMutI CAGTG 2 cut(s) 83, 909
Cac8I GCNNGC 4 cut(s) 73, 77, 629, 976
CaiI CAGNNNCTG 1 cut(s) 85
CfoI GCGC 1 cut(s) 627
Cfr10I RCCGGY 1 cut(s) 218
ClaI ATCGAT 1 cut(s) 398
CseI GACGC 1 cut(s) 476
Csp6I GTAC 2 cut(s) 38, 254
CviAII CATG 2 cut(s) 550, 722
CviQI GTAC 2 cut(s) 38, 254
DdeI CTNAG 3 cut(s) 207, 261, 491
DpnI GATC 2 cut(s) 161, 984
DpnII GATC 2 cut(s) 159, 982
EaeI YGGCCR 2 cut(s) 220, 682
EagI CGGCCG 1 cut(s) 220
EciI GGCGGA 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 656
EclXI CGGCCG 1 cut(s) 220
Eco105I TACGTA 2 cut(s) 844, 1077
Eco147I AGGCCT 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 658
Eco52I CGGCCG 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 596
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 778
EcoICRI GAGCTC 1 cut(s) 656
EcoRII CCWGG 2 cut(s) 65, 823
EcoT22I ATGCAT 1 cut(s) 503
EcoT38I GRGCYC 1 cut(s) 658
Esp3I CGTCTC 1 cut(s) 459
FaeI CATG 2 cut(s) 553, 725
FaqI GGGAC 1 cut(s) 86
FatI CATG 2 cut(s) 549, 721
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 1 cut(s) 159
FblI GTMKAC 2 cut(s) 840, 1073
Fnu4HI GCNGC 9 cut(s) 80, 89, 92, 95, 223, 315, 600, 603, 694
FokI GGATG 3 cut(s) 529, 1002, 1019
FriOI GRGCYC 1 cut(s) 658
Fsp4HI GCNGC 9 cut(s) 80, 89, 92, 95, 223, 315, 600, 603, 694
FspBI CTAG 4 cut(s) 41, 171, 257, 710
GlaI GCGC 1 cut(s) 626
GluI GCNGC 9 cut(s) 80, 89, 92, 95, 223, 315, 600, 603, 694
GsuI CTGGAG 2 cut(s) 393, 846
HaeIII GGCC 3 cut(s) 187, 222, 684
HapII CCGG 2 cut(s) 219, 1065
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 627
Hin1I GRCGYC 1 cut(s) 468
Hin1II CATG 2 cut(s) 553, 725
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HincII GTYRAC 4 cut(s) 199, 417, 799, 868
HindII GTYRAC 4 cut(s) 199, 417, 799, 868
HindIII AAGCTT 2 cut(s) 591, 972
HinfI GANTC 2 cut(s) 125, 334
HpaII CCGG 2 cut(s) 219, 1065
HphI GGTGA 3 cut(s) 134, 208, 220
Hpy166II GTNNAC 7 cut(s) 136, 199, 417, 799, 841, 868, 1074
Hpy188I TCNGA 2 cut(s) 577, 653
Hpy188III TCNNGA 5 cut(s) 300, 362, 614, 894, 1065
Hpy8I GTNNAC 7 cut(s) 136, 199, 417, 799, 841, 868, 1074
HpyAV CCTTC 4 cut(s) 444, 658, 927, 1021
HpyCH4III ACNGT 2 cut(s) 57, 1071
HpyCH4IV ACGT 4 cut(s) 843, 864, 1035, 1076
HpyCH4V TGCA 8 cut(s) 88, 317, 341, 501, 605, 696, 741, 902
HpyF10VI GCNNNNNNNGC 9 cut(s) 85, 88, 94, 347, 492, 599, 690, 732, 984
HpyF3I CTNAG 3 cut(s) 207, 261, 491
HpySE526I ACGT 4 cut(s) 843, 864, 1035, 1076
Hsp92I GRCGYC 1 cut(s) 468
Hsp92II CATG 2 cut(s) 553, 725
HspAI GCGC 1 cut(s) 625
Kpn2I TCCGGA 1 cut(s) 1064
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 2 cut(s) 159, 982
LmnI GCTCC 3 cut(s) 311, 653, 827
Lsp1109I GCAGC 8 cut(s) 91, 100, 103, 106, 301, 586, 589, 680
LweI GCATC 6 cut(s) 350, 359, 488, 510, 838, 997
MaeI CTAG 4 cut(s) 41, 171, 257, 710
MaeII ACGT 4 cut(s) 843, 864, 1035, 1076
MaeIII GTNAC 4 cut(s) 122, 214, 389, 670
MalI GATC 2 cut(s) 161, 984
MboI GATC 2 cut(s) 159, 982
MboII GAAGA 5 cut(s) 103, 496, 602, 758, 950
MfeI CAATTG 4 cut(s) 638, 678, 818, 1097
MhlI GDGCHC 1 cut(s) 658
MlsI TGGCCA 1 cut(s) 684
MluCI AATT 9 cut(s) 178, 379, 541, 638, 678, 687, 802, 818, 1097
MluNI TGGCCA 1 cut(s) 684
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 1043
MnlI CCTC 5 cut(s) 18, 216, 318, 455, 881
Mox20I TGGCCA 1 cut(s) 684
Mph1103I ATGCAT 1 cut(s) 503
MroI TCCGGA 1 cut(s) 1064
MscI TGGCCA 1 cut(s) 684
MseI TTAA 2 cut(s) 537, 786
MslI CAYNNNNRTG 2 cut(s) 398, 767
Msp20I TGGCCA 1 cut(s) 684
MspI CCGG 2 cut(s) 219, 1065
MspR9I CCNGG 2 cut(s) 67, 825
MunI CAATTG 4 cut(s) 638, 678, 818, 1097
Mva1269I GAATGC 1 cut(s) 357
MvaI CCWGG 2 cut(s) 67, 825
MvnI CGCG 1 cut(s) 625
MwoI GCNNNNNNNGC 9 cut(s) 85, 88, 94, 347, 492, 599, 690, 732, 984
NdeI CATATG 1 cut(s) 703
NdeII GATC 2 cut(s) 159, 982
NlaIII CATG 2 cut(s) 553, 725
NlaIV GGNNCC 1 cut(s) 829
NmuCI GTSAC 4 cut(s) 122, 214, 389, 670
NsiI ATGCAT 1 cut(s) 503
NspI RCATGY 1 cut(s) 553
NspV TTCGAA 1 cut(s) 277
PceI AGGCCT 1 cut(s) 187
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 334
PkrI GCNGC 9 cut(s) 81, 90, 93, 96, 224, 316, 601, 604, 695
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
Ppu21I YACGTR 2 cut(s) 844, 1077
Psp124BI GAGCTC 1 cut(s) 658
Psp1406I AACGTT 2 cut(s) 864, 1035
Psp6I CCWGG 2 cut(s) 65, 823
PspGI CCWGG 2 cut(s) 65, 823
PspN4I GGNNCC 1 cut(s) 829
PstI CTGCAG 1 cut(s) 90
PstNI CAGNNNCTG 1 cut(s) 85
RsaI GTAC 2 cut(s) 39, 255
RsaNI GTAC 2 cut(s) 38, 254
RseI CAYNNNNRTG 2 cut(s) 398, 767
SacI GAGCTC 1 cut(s) 658
SaqAI TTAA 2 cut(s) 537, 786
SatI GCNGC 9 cut(s) 80, 89, 92, 95, 223, 315, 600, 603, 694
Sau3AI GATC 2 cut(s) 159, 982
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 67, 825
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 6 cut(s) 350, 359, 488, 510, 838, 997
SfcI CTRYAG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 277
SmiMI CAYNNNNRTG 2 cut(s) 398, 767
SnaBI TACGTA 2 cut(s) 844, 1077
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 9 cut(s) 178, 379, 541, 638, 678, 687, 802, 818, 1097
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 3 cut(s) 223, 290, 813
SspI AATATT 1 cut(s) 1090
SspMI CTAG 4 cut(s) 41, 171, 257, 710
SstI GAGCTC 1 cut(s) 658
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 2 cut(s) 65, 823
TaaI ACNGT 2 cut(s) 57, 1071
TaiI ACGT 4 cut(s) 846, 867, 1038, 1079
TaqI TCGA 6 cut(s) 238, 249, 277, 361, 398, 658
TasI AATT 9 cut(s) 178, 379, 541, 638, 678, 687, 802, 818, 1097
TatI WGTACW 2 cut(s) 37, 253
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 1 cut(s) 334
Tru1I TTAA 2 cut(s) 537, 786
Tru9I TTAA 2 cut(s) 537, 786
TscAI CASTG 2 cut(s) 90, 909
TseFI GTSAC 4 cut(s) 122, 214, 389, 670
TseI GCWGC 8 cut(s) 79, 88, 91, 94, 314, 599, 602, 693
Tsp45I GTSAC 4 cut(s) 122, 214, 389, 670
TspDTI ATGAA 7 cut(s) 103, 381, 416, 489, 710, 720, 759
TspRI CASTG 2 cut(s) 90, 909
XceI RCATGY 1 cut(s) 553
XmiI GTMKAC 2 cut(s) 840, 1073
XspI CTAG 4 cut(s) 41, 171, 257, 710
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.