MD08G1215700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016047
Physical Location & Seq
Forward (+)
27849350 .. 27851567
2218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1215700.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGAGCAGGGCTAGAAAATTCTGCGACTTGGGTTTGTTTGCAATAGTTATAGTAGTTGTGTGGCTGCAGGGTTTGAGTGGCAGAGTTTGGTCGCAGCCGCAGTCGACAGCCCCAGTGCCTGGTTTCTTCATTTTTGGTGACTCGTTGGTTGACAATGGGAACAACAATGGGATAATAACACTTTCTAGGGCAAATTATAGACCTTATGGCATCGACTTCCCTCTTGGTGTAACTGGCCGCTTCACCAATGGTAGAACCTATGTTGATGTCCTAGCTCAGCTTCTTGGATTTCGAACTTATATTCCGCCCTATTCAAGAACACGCGGAGATGCACTGCTGAGGGGAGCCAATTTTGCATCCGGTGCAGCTGGTATCCGAGAGGAAACGGGAGACAATCTAGGTGCTCACATGTCCATGAACCAACAAGTTGCAAACTTTGGGAATGCAGTGCTAGAAATGAGAAGATTCTTTAGAGGAGATGCCAATGCACTCAACACCTATCTTAGCAGATGCATCTTCTATTCTGGGATGGGAAGTAATGACTATCTTAACAACTATTTTATGCCCAGCTTCTATACATCTAGCTCCGATTACACCACGCAAGCGTATGCTGCTGTACTCCTTCAGGACTACTCGCGTCAACTCACGCAATTATATTCACTGGGAGCTCGGAAGGTGATTGTAACTGCTGTTGGCCAAATCGGTTGCATACCATATCAACTAGCAAGGTTCAATAGCAACAGCAGCCGCTGCAACGAAAACATCAACAAGGCTATCCTTATTTTCAACTCAGGCCTCAAAAAACTGGTTGACAACTTCAATGGTGGTCAATTGCCTGGGGCCAAATTTGTCTACCTCGATGCTTATCAAAGCACCTTTGATCTGTTTCAAAATCCCACTACTAACGGGTTTGAAGTGATAGACAAGGGTTGTTGTGGAGTAGGAAGGAACAATGGGCAGATAACTTGTCTACCTCTTCAGCAAACTTGTACGGATCGCACAAAGTACTTGTTTTGGGATGCCTTCCATCCAACAGAGGTAGCAAACATTTTGCTGGGCAAGGCATCATTCAGTGGTTCCAATCAACAATCATATGCTTACCCAATCAATATTCAACAACTGGCAATGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

41.68

Weight (kDa)

9.03

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 354 9.6e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33811
fragaria_vesca FvH4_5g32900
malus_domestica MD08G1215700.v1.1
prunus_persica Prupe.1G547400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0230871
rosa_laevigata RLG00000001456
rosa_multiflora Rmu_sc0000551.1_g000017 Rmu_sc0010407.1_g000024
rosa_roxburghii Rroxscaffold_3G00229730
rosa_rugosa Rorug07G0262700
rosa_samantha Rh7BG392000 Rh7CG434900 Rh7DG410400
rosa_wichuraiana Rw7G034290 Rw7G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 119
AccI GTMKAC 3 cut(s) 104, 852, 970
AccII CGCG 2 cut(s) 324, 637
AciI CCGC 5 cut(s) 98, 238, 305, 324, 748
AclWI GGATC 1 cut(s) 1002
AcoI YGGCCR 2 cut(s) 235, 694
AcsI RAATTY 2 cut(s) 17, 845
AcuI CTGAAG 2 cut(s) 608, 962
AfaI GTAC 3 cut(s) 618, 991, 1007
AfiI CCNNNNNNNGG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 7 cut(s) 315, 733, 787, 820, 890, 914, 1115
AjnI CCWGG 2 cut(s) 118, 835
AloI GAACNNNNNNTCC 2 cut(s) 286, 318
AluBI AGCT 6 cut(s) 275, 280, 368, 570, 585, 668
AluI AGCT 6 cut(s) 275, 280, 368, 570, 585, 668
Alw21I GWGCWC 2 cut(s) 406, 670
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 1 cut(s) 1002
AlwNI CAGNNNCTG 2 cut(s) 119, 750
AoxI GGCC 4 cut(s) 235, 694, 793, 840
ApeKI GCWGC 6 cut(s) 64, 94, 365, 611, 744, 750
ApoI RAATTY 2 cut(s) 17, 845
AspS9I GGNCC 1 cut(s) 840
AsuHPI GGTGA 3 cut(s) 149, 235, 688
AsuII TTCGAA 1 cut(s) 292
BalI TGGCCA 1 cut(s) 696
BanII GRGCYC 1 cut(s) 670
Bbv12I GWGCWC 2 cut(s) 406, 670
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 6 cut(s) 51, 106, 377, 598, 737, 756
BccI CCATC 2 cut(s) 523, 1035
BciT130I CCWGG 2 cut(s) 120, 837
BciVI GTATCC 1 cut(s) 383
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 7 cut(s) 12, 186, 272, 398, 452, 582, 722
BfmI CTRYAG 1 cut(s) 65
BfuI GTATCC 1 cut(s) 383
BisI GCNGC 9 cut(s) 65, 95, 98, 238, 366, 612, 745, 748, 751
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 9 cut(s) 66, 96, 99, 239, 367, 613, 746, 749, 752
BmcAI AGTACT 1 cut(s) 1007
Bme1390I CCNGG 2 cut(s) 120, 837
BmgT120I GGNCC 1 cut(s) 840
BmiI GGNNCC 3 cut(s) 346, 841, 1078
BmrFI CCNGG 2 cut(s) 120, 837
BmrI ACTGGG 2 cut(s) 107, 671
BmsI GCATC 9 cut(s) 219, 319, 365, 469, 500, 522, 850, 1009, 1073
BmuI ACTGGG 2 cut(s) 107, 671
Bpu10I CCTNAGC 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 292
BsaBI GATNNNNATC 1 cut(s) 864
BsaJI CCNNGG 1 cut(s) 836
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse1I ACTGG 5 cut(s) 113, 238, 666, 810, 1125
Bse3DI GCAATG 1 cut(s) 1131
Bse8I GATNNNNATC 1 cut(s) 864
BseBI CCWGG 2 cut(s) 120, 837
BseDI CCNNGG 1 cut(s) 836
BseGI GGATG 4 cut(s) 356, 534, 1024, 1027
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 3 cut(s) 290, 329, 804
BseNI ACTGG 5 cut(s) 113, 238, 666, 810, 1125
BseRI GAGGAG 1 cut(s) 489
BseXI GCAGC 6 cut(s) 51, 106, 377, 598, 737, 756
BseYI CCCAGC 2 cut(s) 566, 1054
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 2 cut(s) 324, 637
BshFI GGCC 4 cut(s) 237, 696, 795, 842
BsiHKAI GWGCWC 2 cut(s) 406, 670
BsiSI CCGG 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 384
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 4 cut(s) 237, 696, 795, 842
Bsp119I TTCGAA 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 406, 670
Bsp143I GATC 2 cut(s) 880, 994
Bsp1720I GCTNAGC 1 cut(s) 276
BspACI CCGC 5 cut(s) 98, 238, 305, 324, 748
BspANI GGCC 4 cut(s) 237, 696, 795, 842
BspCNI CTCAG 3 cut(s) 289, 330, 803
BspFNI CGCG 2 cut(s) 324, 637
BspLI GGNNCC 3 cut(s) 346, 841, 1078
BspMAI CTGCAG 1 cut(s) 69
BspPI GGATC 1 cut(s) 1002
BspT104I TTCGAA 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 1131
BsrI ACTGG 5 cut(s) 113, 238, 666, 810, 1125
BssECI CCNNGG 1 cut(s) 836
BssMI GATC 2 cut(s) 880, 994
Bst2UI CCWGG 2 cut(s) 120, 837
Bst6I CTCTTC 1 cut(s) 981
BstAPI GCANNNNNTGC 2 cut(s) 362, 750
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 4 cut(s) 276, 338, 503, 790
BstF5I GGATG 4 cut(s) 356, 534, 1024, 1027
BstFNI CGCG 2 cut(s) 324, 637
BstKTI GATC 2 cut(s) 883, 997
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 2 cut(s) 880, 994
BstMWI GCNNNNNNNGC 5 cut(s) 353, 362, 611, 744, 750
BstNI CCWGG 2 cut(s) 120, 837
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 118, 835
BstSFI CTRYAG 1 cut(s) 65
BstUI CGCG 2 cut(s) 324, 637
BstV1I GCAGC 6 cut(s) 51, 106, 377, 598, 737, 756
BsuI GTATCC 1 cut(s) 383
BsuRI GGCC 4 cut(s) 237, 696, 795, 842
BtsCI GGATG 4 cut(s) 356, 534, 1024, 1027
BtsI GCAGTG 2 cut(s) 332, 453
BtsIMutI CAGTG 5 cut(s) 120, 332, 453, 659, 1078
Cac8I GCNNGC 1 cut(s) 603
CaiI CAGNNNCTG 2 cut(s) 119, 750
Cfr13I GGNCC 1 cut(s) 840
CseI GACGC 1 cut(s) 626
Csp6I GTAC 3 cut(s) 617, 990, 1006
CviAII CATG 2 cut(s) 409, 415
CviQI GTAC 3 cut(s) 617, 990, 1006
DdeI CTNAG 4 cut(s) 276, 338, 503, 790
DpnI GATC 2 cut(s) 882, 996
DpnII GATC 2 cut(s) 880, 994
EaeI YGGCCR 2 cut(s) 235, 694
Eam1104I CTCTTC 1 cut(s) 981
EarI CTCTTC 1 cut(s) 981
EciI GGCGGA 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 668
Eco147I AGGCCT 1 cut(s) 795
Eco24I GRGCYC 1 cut(s) 670
Eco53kI GAGCTC 1 cut(s) 668
Eco57I CTGAAG 2 cut(s) 608, 962
EcoICRI GAGCTC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 118, 835
EcoT22I ATGCAT 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 670
FaeI CATG 2 cut(s) 412, 418
FatI CATG 2 cut(s) 408, 414
FauNDI CATATG 1 cut(s) 1093
FblI GTMKAC 3 cut(s) 104, 852, 970
Fnu4HI GCNGC 9 cut(s) 65, 95, 98, 238, 366, 612, 745, 748, 751
FokI GGATG 4 cut(s) 343, 541, 1014, 1031
FriOI GRGCYC 1 cut(s) 670
Fsp4HI GCNGC 9 cut(s) 65, 95, 98, 238, 366, 612, 745, 748, 751
FspBI CTAG 7 cut(s) 12, 186, 272, 398, 452, 582, 722
GluI GCNGC 9 cut(s) 65, 95, 98, 238, 366, 612, 745, 748, 751
GsaI CCCAGC 2 cut(s) 570, 1058
HaeIII GGCC 4 cut(s) 237, 696, 795, 842
HapII CCGG 1 cut(s) 360
HgaI GACGC 1 cut(s) 626
Hin1II CATG 2 cut(s) 412, 418
HincII GTYRAC 4 cut(s) 105, 151, 641, 811
HindII GTYRAC 4 cut(s) 105, 151, 641, 811
HinfI GANTC 2 cut(s) 140, 465
HpaII CCGG 1 cut(s) 360
HphI GGTGA 3 cut(s) 149, 235, 688
Hpy166II GTNNAC 6 cut(s) 105, 151, 641, 811, 853, 971
Hpy188I TCNGA 3 cut(s) 377, 589, 672
Hpy188III TCNNGA 2 cut(s) 315, 626
Hpy8I GTNNAC 6 cut(s) 105, 151, 641, 811, 853, 971
HpyAV CCTTC 4 cut(s) 632, 667, 939, 1033
HpyF10VI GCNNNNNNNGC 5 cut(s) 353, 362, 611, 744, 750
HpyF3I CTNAG 4 cut(s) 276, 338, 503, 790
Hsp92II CATG 2 cut(s) 412, 418
Kzo9I GATC 2 cut(s) 880, 994
LmnI GCTCC 3 cut(s) 344, 590, 665
Lsp1109I GCAGC 6 cut(s) 51, 106, 377, 598, 737, 756
LweI GCATC 9 cut(s) 219, 319, 365, 469, 500, 522, 850, 1009, 1073
MaeI CTAG 7 cut(s) 12, 186, 272, 398, 452, 582, 722
MaeIII GTNAC 3 cut(s) 137, 229, 682
MalI GATC 2 cut(s) 882, 996
MboI GATC 2 cut(s) 880, 994
MboII GAAGA 4 cut(s) 118, 474, 508, 968
MfeI CAATTG 1 cut(s) 830
MhlI GDGCHC 2 cut(s) 406, 670
MlsI TGGCCA 1 cut(s) 696
MluCI AATT 6 cut(s) 17, 193, 349, 650, 830, 845
MluNI TGGCCA 1 cut(s) 696
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 1055
MnlI CCTC 8 cut(s) 231, 333, 373, 467, 806, 866, 984, 1030
Mox20I TGGCCA 1 cut(s) 696
Mph1103I ATGCAT 1 cut(s) 515
MscI TGGCCA 1 cut(s) 696
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 413
Msp20I TGGCCA 1 cut(s) 696
MspA1I CMGCKG 2 cut(s) 368, 750
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 2 cut(s) 120, 837
MunI CAATTG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 448
MvaI CCWGG 2 cut(s) 120, 837
MvnI CGCG 2 cut(s) 324, 637
MwoI GCNNNNNNNGC 5 cut(s) 353, 362, 611, 744, 750
NdeI CATATG 1 cut(s) 1093
NdeII GATC 2 cut(s) 880, 994
NlaIII CATG 2 cut(s) 412, 418
NlaIV GGNNCC 3 cut(s) 346, 841, 1078
NmuCI GTSAC 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 1 cut(s) 412
NspV TTCGAA 1 cut(s) 292
PceI AGGCCT 1 cut(s) 795
PciI ACATGT 1 cut(s) 408
PctI GAATGC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 465
PflMI CCANNNNNTGG 1 cut(s) 119
PkrI GCNGC 9 cut(s) 66, 96, 99, 239, 367, 613, 746, 749, 752
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
PscI ACATGT 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 670
Psp6I CCWGG 2 cut(s) 118, 835
PspFI CCCAGC 2 cut(s) 566, 1054
PspGI CCWGG 2 cut(s) 118, 835
PspN4I GGNNCC 3 cut(s) 346, 841, 1078
PspPI GGNCC 1 cut(s) 840
PstI CTGCAG 1 cut(s) 69
PstNI CAGNNNCTG 2 cut(s) 119, 750
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 3 cut(s) 618, 991, 1007
RsaNI GTAC 3 cut(s) 617, 990, 1006
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 1 cut(s) 670
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 9 cut(s) 65, 95, 98, 238, 366, 612, 745, 748, 751
Sau3AI GATC 2 cut(s) 880, 994
Sau96I GGNCC 1 cut(s) 840
ScaI AGTACT 1 cut(s) 1007
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 120, 837
SduI GDGCHC 2 cut(s) 406, 670
SfaNI GCATC 9 cut(s) 219, 319, 365, 469, 500, 522, 850, 1009, 1073
SfcI CTRYAG 1 cut(s) 65
SfuI TTCGAA 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 6 cut(s) 17, 193, 349, 650, 830, 845
SseBI AGGCCT 1 cut(s) 795
SsiI CCGC 5 cut(s) 98, 238, 305, 324, 748
SspI AATATT 1 cut(s) 1111
SspMI CTAG 7 cut(s) 12, 186, 272, 398, 452, 582, 722
SstI GAGCTC 1 cut(s) 670
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 2 cut(s) 118, 835
TaqI TCGA 4 cut(s) 104, 213, 292, 858
TasI AATT 6 cut(s) 17, 193, 349, 650, 830, 845
TatI WGTACW 2 cut(s) 616, 1005
TauI GCSGC 3 cut(s) 100, 240, 750
TfiI GAWTC 1 cut(s) 465
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 5 cut(s) 120, 339, 453, 666, 1078
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 6 cut(s) 64, 94, 365, 611, 744, 750
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 2 cut(s) 118, 431
TspGWI ACGGA 1 cut(s) 1007
TspRI CASTG 5 cut(s) 120, 339, 453, 666, 1078
Van91I CCANNNNNTGG 1 cut(s) 119
XapI RAATTY 2 cut(s) 17, 845
XceI RCATGY 1 cut(s) 412
XmiI GTMKAC 3 cut(s) 104, 852, 970
XspI CTAG 7 cut(s) 12, 186, 272, 398, 452, 582, 722
ZrmI AGTACT 1 cut(s) 1007
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.