Prupe.1G547400_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016047
Physical Location & Seq
Forward (+)
44738796 .. 44741304
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G547400.1

Sequence Viewer

Length: 1110 bp
ATGAGGAAATTTTGTGACTTGGGTTTGTTGTTAATTGTTGCTCTTTGGCTAGGGTTCAATGGCAGGGCTTGGTCACAGCCACAACAGGCACAAGTTCCTGGTTTCTTCATATTTGGTGACTCATTGGTAGACAATGGAAACAATAATGGCATTGTCACTCTTGCTAGGGCCAACTATAGGCCTTATGGCATTGACTTCCCTCAGGGTGTCACTGGCCGCTTCACTAATGGAAGAACCTATGTTGATGTTCTAGCTCAGCTTCTGGGGTTTCAAACTTATATTCCGCCTTACTCAAGAACTCGCGGAGCTGCACTTCTGAGGGGAGTGAATTATGCATCAGGCGCAGCTGGAATTCGCGATGAAACGGGAGATAATCTGGGTGCTCACACATCCATGAACCAACAAGTTTCAAACTTTGGCAATACAGTGGCAGAAATGAGGAGGTTCTTCAGAGGAGATGCCAATGCACTCAGCAACTATCTTGGAAGATGCATCTTCTATTCTGGGATGGGAAGTAATGACTATCTTAACAACTATTTTATGCCAAACTTCTATACATCTAGTTCTGATTACACTACGAAAGCTTACGCTGCCGCACTCCTTCAGGACTATTCGCGCCAGCTGAGCCAATTGTATGCATTGGGAGCTCGGAAGGTGATTGTGACTGGGGTTGGCCAAATCGGTTGCATACCTTATCAACTAGCAAGGTATCATAGCAATAGCAGCCGCTGCAATGAAAACATCAACAATGCCATCCTTATTTTCAACTCGGGACTTAAAAAGATGGTTGACAATTTCAATCGTGGTCAATTGCCTGGAGCCAAGTTTGTCTACCTAGATTCTTATCAAAGCACCTTTGATCTGTTTCAAAATGCATCTGCTGCTGGTTTTGAAGTGATAGATAAAGGATGTTGTGGTGTAGGAAGGAACAATGGGCAGATAACTTGTCTGCCTCTTCAACAAGCTTGTAGAGATCGCACAAAGTACATGTTTTGGGATGCCTTTCATCCAACAGAAGCAGCAAACATTCTGCTAGGCAAGGCATCATACAGTTCCAATACACAGTCATATACTTGTCCAATTAATATACAACAATTGGCTATGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

40.82

Weight (kDa)

9.06

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33811
fragaria_vesca FvH4_5g32900
malus_domestica MD08G1215700.v1.1
prunus_persica Prupe.1G547400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0230871
rosa_laevigata RLG00000001456
rosa_multiflora Rmu_sc0000551.1_g000017 Rmu_sc0010407.1_g000024
rosa_roxburghii Rroxscaffold_3G00229730
rosa_rugosa Rorug07G0262700
rosa_samantha Rh7BG392000 Rh7CG434900 Rh7DG410400
rosa_wichuraiana Rw7G034290 Rw7G034450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 129, 831
AccII CGCG 3 cut(s) 303, 357, 616
AciI CCGC 5 cut(s) 217, 284, 303, 594, 727
AcoI YGGCCR 2 cut(s) 214, 673
AcsI RAATTY 2 cut(s) 8, 351
AcuI CTGAAG 2 cut(s) 433, 587
AfaI GTAC 1 cut(s) 986
AfiI CCNNNNNNNGG 1 cut(s) 177
AflIII ACRYGT 1 cut(s) 987
AgsI TTSAA 8 cut(s) 58, 272, 411, 766, 799, 869, 893, 959
AjnI CCWGG 2 cut(s) 97, 814
AluBI AGCT 8 cut(s) 254, 259, 308, 347, 584, 622, 647, 965
AluI AGCT 8 cut(s) 254, 259, 308, 347, 584, 622, 647, 965
Alw21I GWGCWC 2 cut(s) 385, 649
AlwNI CAGNNNCTG 2 cut(s) 262, 729
Ama87I CYCGRG 1 cut(s) 769
AoxI GGCC 4 cut(s) 168, 179, 214, 673
ApeKI GCWGC 7 cut(s) 308, 344, 590, 723, 729, 881, 1019
ApoI RAATTY 2 cut(s) 8, 351
AseI ATTAAT 1 cut(s) 1083
AspLEI GCGC 2 cut(s) 344, 618
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 128, 667
AvaI CYCGRG 1 cut(s) 769
AxyI CCTNAGG 1 cut(s) 201
BalI TGGCCA 1 cut(s) 675
BanII GRGCYC 1 cut(s) 649
Bbv12I GWGCWC 2 cut(s) 385, 649
BbvI GCAGC 7 cut(s) 295, 356, 577, 716, 735, 868, 1031
BccI CCATC 3 cut(s) 502, 761, 778
BciT130I CCWGG 2 cut(s) 99, 816
BfaI CTAG 7 cut(s) 50, 165, 251, 561, 701, 836, 1034
BfmI CTRYAG 1 cut(s) 175
BlpI GCTNAGC 2 cut(s) 255, 623
Bme1390I CCNGG 2 cut(s) 99, 816
BmeT110I CYCGRG 1 cut(s) 769
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 1 cut(s) 820
BmrFI CCNGG 2 cut(s) 99, 816
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 7 cut(s) 344, 448, 479, 501, 884, 988, 1052
BmuI ACTGGG 1 cut(s) 675
BpmI CTGGAG 1 cut(s) 837
Bpu1102I GCTNAGC 2 cut(s) 255, 623
BpuEI CTTGAG 1 cut(s) 277
BsaBI GATNNNNATC 1 cut(s) 843
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 217, 670
Bse21I CCTNAGG 1 cut(s) 201
Bse3DI GCAATG 1 cut(s) 739
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 2 cut(s) 99, 816
BseGI GGATG 6 cut(s) 389, 513, 753, 914, 1003, 1006
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 1 cut(s) 739
BseMII CTCAG 5 cut(s) 215, 269, 308, 484, 614
BseNI ACTGG 2 cut(s) 217, 670
BseRI GAGGAG 2 cut(s) 454, 468
BseXI GCAGC 7 cut(s) 295, 356, 577, 716, 735, 868, 1031
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 3 cut(s) 303, 357, 616
BshFI GGCC 4 cut(s) 170, 181, 216, 675
BsiHKAI GWGCWC 2 cut(s) 385, 649
BsiHKCI CYCGRG 1 cut(s) 769
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 786
BsnI GGCC 4 cut(s) 170, 181, 216, 675
BsoBI CYCGRG 1 cut(s) 769
Bsp1286I GDGCHC 2 cut(s) 385, 649
Bsp143I GATC 2 cut(s) 859, 973
Bsp1720I GCTNAGC 2 cut(s) 255, 623
Bsp68I TCGCGA 1 cut(s) 357
BspACI CCGC 5 cut(s) 217, 284, 303, 594, 727
BspANI GGCC 4 cut(s) 170, 181, 216, 675
BspCNI CTCAG 5 cut(s) 214, 268, 309, 483, 615
BspFNI CGCG 3 cut(s) 303, 357, 616
BspLI GGNNCC 1 cut(s) 820
BsrDI GCAATG 1 cut(s) 739
BsrI ACTGG 2 cut(s) 217, 670
BssMI GATC 2 cut(s) 859, 973
Bst2UI CCWGG 2 cut(s) 99, 816
Bst4CI ACNGT 3 cut(s) 427, 1052, 1065
Bst6I CTCTTC 1 cut(s) 960
BstAPI GCANNNNNTGC 2 cut(s) 729, 881
BstC8I GCNNGC 1 cut(s) 620
BstDEI CTNAG 5 cut(s) 201, 255, 317, 470, 623
BstF5I GGATG 6 cut(s) 389, 513, 753, 914, 1003, 1006
BstFNI CGCG 3 cut(s) 303, 357, 616
BstHHI GCGC 2 cut(s) 344, 618
BstKTI GATC 2 cut(s) 862, 976
BstMBI GATC 2 cut(s) 859, 973
BstMWI GCNNNNNNNGC 7 cut(s) 341, 590, 624, 644, 723, 729, 881
BstNI CCWGG 2 cut(s) 99, 816
BstNSI RCATGY 1 cut(s) 991
BstSCI CCNGG 2 cut(s) 97, 814
BstSFI CTRYAG 1 cut(s) 175
BstUI CGCG 3 cut(s) 303, 357, 616
BstV1I GCAGC 7 cut(s) 295, 356, 577, 716, 735, 868, 1031
Bsu36I CCTNAGG 1 cut(s) 201
BsuRI GGCC 4 cut(s) 170, 181, 216, 675
BtgZI GCGATG 1 cut(s) 372
BtsCI GGATG 6 cut(s) 389, 513, 753, 914, 1003, 1006
BtsIMutI CAGTG 2 cut(s) 210, 432
BtuMI TCGCGA 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 620
CaiI CAGNNNCTG 2 cut(s) 262, 729
CfoI GCGC 2 cut(s) 344, 618
Cfr13I GGNCC 1 cut(s) 168
Csp6I GTAC 1 cut(s) 985
CviAII CATG 2 cut(s) 394, 988
CviQI GTAC 1 cut(s) 985
DdeI CTNAG 5 cut(s) 201, 255, 317, 470, 623
DpnI GATC 2 cut(s) 861, 975
DpnII GATC 2 cut(s) 859, 973
EaeI YGGCCR 2 cut(s) 214, 673
Eam1104I CTCTTC 1 cut(s) 960
EarI CTCTTC 1 cut(s) 960
EciI GGCGGA 1 cut(s) 273
Ecl136II GAGCTC 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 647
Eco57I CTGAAG 2 cut(s) 433, 587
Eco81I CCTNAGG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 769
EcoICRI GAGCTC 1 cut(s) 647
EcoRI GAATTC 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 97, 814
EcoT22I ATGCAT 4 cut(s) 337, 494, 640, 877
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 2 cut(s) 397, 991
FaqI GGGAC 1 cut(s) 786
FatI CATG 2 cut(s) 393, 987
FblI GTMKAC 2 cut(s) 129, 831
FokI GGATG 6 cut(s) 376, 520, 740, 921, 993, 1010
FriOI GRGCYC 1 cut(s) 649
FspBI CTAG 7 cut(s) 50, 165, 251, 561, 701, 836, 1034
GlaI GCGC 2 cut(s) 343, 617
GsuI CTGGAG 1 cut(s) 837
HaeIII GGCC 4 cut(s) 170, 181, 216, 675
HhaI GCGC 2 cut(s) 344, 618
Hin1II CATG 2 cut(s) 397, 991
Hin6I GCGC 2 cut(s) 342, 616
HinP1I GCGC 2 cut(s) 342, 616
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 2 cut(s) 582, 963
HinfI GANTC 2 cut(s) 119, 839
HphI GGTGA 2 cut(s) 128, 667
Hpy166II GTNNAC 3 cut(s) 130, 790, 832
Hpy188I TCNGA 4 cut(s) 318, 452, 568, 651
Hpy188III TCNNGA 4 cut(s) 294, 356, 605, 771
Hpy8I GTNNAC 3 cut(s) 130, 790, 832
HpyAV CCTTC 3 cut(s) 611, 646, 918
HpyCH4III ACNGT 3 cut(s) 427, 1052, 1065
HpyCH4V TGCA 8 cut(s) 311, 335, 467, 492, 638, 687, 732, 875
HpyF10VI GCNNNNNNNGC 7 cut(s) 341, 590, 624, 644, 723, 729, 881
HpyF3I CTNAG 5 cut(s) 201, 255, 317, 470, 623
Hsp92II CATG 2 cut(s) 397, 991
HspAI GCGC 2 cut(s) 342, 616
Kzo9I GATC 2 cut(s) 859, 973
LmnI GCTCC 3 cut(s) 305, 644, 818
Lsp1109I GCAGC 7 cut(s) 295, 356, 577, 716, 735, 868, 1031
LweI GCATC 7 cut(s) 344, 448, 479, 501, 884, 988, 1052
MaeI CTAG 7 cut(s) 50, 165, 251, 561, 701, 836, 1034
MaeIII GTNAC 6 cut(s) 14, 72, 116, 154, 208, 661
MalI GATC 2 cut(s) 861, 975
MboI GATC 2 cut(s) 859, 973
MboII GAAGA 6 cut(s) 97, 243, 439, 487, 498, 947
MfeI CAATTG 3 cut(s) 629, 809, 1094
MhlI GDGCHC 2 cut(s) 385, 649
MlsI TGGCCA 1 cut(s) 675
MluCI AATT 9 cut(s) 8, 33, 328, 351, 629, 793, 809, 1080, 1094
MluNI TGGCCA 1 cut(s) 675
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 1034
MnlI CCTC 6 cut(s) 210, 312, 432, 435, 446, 963
Mox20I TGGCCA 1 cut(s) 675
Mph1103I ATGCAT 4 cut(s) 337, 494, 640, 877
MscI TGGCCA 1 cut(s) 675
MseI TTAA 4 cut(s) 32, 528, 777, 1083
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 675
MspA1I CMGCKG 3 cut(s) 347, 622, 729
MspR9I CCNGG 2 cut(s) 99, 816
MunI CAATTG 3 cut(s) 629, 809, 1094
MvaI CCWGG 2 cut(s) 99, 816
MvnI CGCG 3 cut(s) 303, 357, 616
MwoI GCNNNNNNNGC 7 cut(s) 341, 590, 624, 644, 723, 729, 881
NdeII GATC 2 cut(s) 859, 973
NlaIII CATG 2 cut(s) 397, 991
NlaIV GGNNCC 1 cut(s) 820
NmuCI GTSAC 6 cut(s) 14, 72, 116, 154, 208, 661
NruI TCGCGA 1 cut(s) 357
NsiI ATGCAT 4 cut(s) 337, 494, 640, 877
NspI RCATGY 1 cut(s) 991
PceI AGGCCT 1 cut(s) 181
PciI ACATGT 1 cut(s) 987
PfeI GAWTC 1 cut(s) 839
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PscI ACATGT 1 cut(s) 987
PshBI ATTAAT 1 cut(s) 1083
Psp124BI GAGCTC 1 cut(s) 649
Psp6I CCWGG 2 cut(s) 97, 814
PspGI CCWGG 2 cut(s) 97, 814
PspN4I GGNNCC 1 cut(s) 820
PspPI GGNCC 1 cut(s) 168
PstNI CAGNNNCTG 2 cut(s) 262, 729
PvuII CAGCTG 2 cut(s) 347, 622
RruI TCGCGA 1 cut(s) 357
RsaI GTAC 1 cut(s) 986
RsaNI GTAC 1 cut(s) 985
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 1 cut(s) 649
SaqAI TTAA 4 cut(s) 32, 528, 777, 1083
Sau3AI GATC 2 cut(s) 859, 973
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 2 cut(s) 99, 816
SduI GDGCHC 2 cut(s) 385, 649
SfaNI GCATC 7 cut(s) 344, 448, 479, 501, 884, 988, 1052
SfcI CTRYAG 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
Sse9I AATT 9 cut(s) 8, 33, 328, 351, 629, 793, 809, 1080, 1094
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 5 cut(s) 217, 284, 303, 594, 727
SspMI CTAG 7 cut(s) 50, 165, 251, 561, 701, 836, 1034
SstI GAGCTC 1 cut(s) 649
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 97, 814
TaaI ACNGT 3 cut(s) 427, 1052, 1065
TasI AATT 9 cut(s) 8, 33, 328, 351, 629, 793, 809, 1080, 1094
TatI WGTACW 1 cut(s) 984
TauI GCSGC 3 cut(s) 219, 596, 729
TfiI GAWTC 1 cut(s) 839
Tru1I TTAA 4 cut(s) 32, 528, 777, 1083
Tru9I TTAA 4 cut(s) 32, 528, 777, 1083
TscAI CASTG 2 cut(s) 217, 432
TseFI GTSAC 6 cut(s) 14, 72, 116, 154, 208, 661
TseI GCWGC 7 cut(s) 308, 344, 590, 723, 729, 881, 1019
Tsp45I GTSAC 6 cut(s) 14, 72, 116, 154, 208, 661
TspDTI ATGAA 5 cut(s) 97, 375, 410, 750, 995
TspRI CASTG 2 cut(s) 217, 432
VspI ATTAAT 1 cut(s) 1083
XapI RAATTY 2 cut(s) 8, 351
XceI RCATGY 1 cut(s) 991
XmiI GTMKAC 2 cut(s) 129, 831
XspI CTAG 7 cut(s) 50, 165, 251, 561, 701, 836, 1034
Zsp2I ATGCAT 4 cut(s) 337, 494, 640, 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.