FvH4_6g04400

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017322
Physical Location & Seq
Forward (+)
2397836 .. 2399744
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04400.t1

Sequence Viewer

Length: 1149 bp
ATGGAGAGGATCGCGGCGGAGTTACAGGACAGCGTGGTCGACTTGACCCGGCAGTTAACGGAGCTCCAGGCCCGCTACGACTCGACCCGGAACCAGCTCCCGACCCGAATGCAGGCTCTGGACCAGAGGGAGCTGCAATTGCAGTCCAGGGAGCTGAGGGTGAAGTCGGAAGCGGAAACAGTCCGAAAAAAGTGGGAGATGTTAGAGATGAGGGAGAGGGAGCTTGAGATGAGGGAGTTGGAGAGAGAAGAGCATTTGGTGATGCTGGAGAAGTCGGTGGAGGAGAGGGAGAGGGGTTTGGATGGGATGCAGGAGTCGCTGGAGGAGCAGAGGCGGCATTCGAAGGCGCTGCAGGAGGTTTTGGAGGAGAAATTGGGGTTGATTTCTGGCCAAATGAGGGTGAGGCTGAGAGAGTTTGAAGAGAAGGCGAGAGAGGTTGGAGTGAAGGTTAGTGGGTGTGGTGATGATGAGGGAGGAGGAGGAGGTTGCGAAATGCGGGGTGGTTTGAATGCAGGGGAGGTTGTGAAGCTTATTGGGGAGATTTCGCGGAGTGCAGAGATTGTGGAGTTGTGTCAGGGAGTTGATTGTGTTGATAAGATGCAGGATGTTATCCGGATTCTCATTGAACGAAAGCAACTGATAGAGGCTGTTGGATTTATATGCACCTTCAAGTTCACCGATGAGTTCCCCCCTGTACCACTCTTAAATGAGTATGTGGAGGATGCAGAGAAGTGGTGGACTGAAACTTTCAGTCAGAAGAAATCACTTCGTAAGAAGGAAAATGCTGTAGATGATCGAATAACTAGTCTAAGAGCTGTGGCTCAATGTGTCAAAGCTTACAACTTACAGTCTGAGTATTCACTAGTGCACATTGACAATGACATATTGGAGCTTGAAAATCTAAAGGAAAATCTGCATTCGGTGGCATCTCTCAACATTATGATGAAAACTAAAGGATTTGGAAAACTAAACGAGAATAGGGAGGTGGTCCTATGTCTTCCGTCATATAATAGACAAGAGCAGAGAAACCAGAGGAAAGAAGATTTCAGCACTCTTTCCCCCAGTTTTCGCATACATCGCTGTTGCAGAAAAAAAATTAATCGGCCAGCCATTTCAACTGCTAGCCCCATGACTCGGATAGAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

383

Amino Acids

44.28

Weight (kDa)

5.48

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 171 - 306 1e-30 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
AccI GTMKAC 1 cut(s) 39
AccII CGCG 2 cut(s) 14, 547
AccIII TCCGGA 1 cut(s) 612
AciI CCGC 7 cut(s) 14, 17, 73, 173, 334, 496, 547
AclWI GGATC 1 cut(s) 17
AcoI YGGCCR 2 cut(s) 388, 1103
AfaI GTAC 1 cut(s) 696
AfiI CCNNNNNNNGG 3 cut(s) 112, 397, 1134
AgsI TTSAA 6 cut(s) 419, 508, 626, 670, 896, 1116
AhlI ACTAGT 2 cut(s) 803, 862
AjnI CCWGG 2 cut(s) 66, 146
AluBI AGCT 9 cut(s) 64, 97, 133, 154, 223, 529, 815, 836, 892
AluI AGCT 9 cut(s) 64, 97, 133, 154, 223, 529, 815, 836, 892
Alw21I GWGCWC 2 cut(s) 66, 870
Alw44I GTGCAC 1 cut(s) 866
AlwI GGATC 1 cut(s) 17
AlwNI CAGNNNCTG 1 cut(s) 118
Aor13HI TCCGGA 1 cut(s) 612
AoxI GGCC 3 cut(s) 69, 388, 1103
ApaLI GTGCAC 1 cut(s) 866
ApeKI GCWGC 2 cut(s) 133, 349
ArsI GACNNNNNNTTYG 2 cut(s) 790, 822
AseI ATTAAT 1 cut(s) 1098
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 3 cut(s) 70, 121, 988
AsuC2I CCSGG 2 cut(s) 49, 88
AsuHPI GGTGA 5 cut(s) 172, 271, 412, 473, 667
AsuII TTCGAA 1 cut(s) 341
AsuNHI GCTAGC 1 cut(s) 1121
AvaII GGWCC 2 cut(s) 121, 988
BaeGI GKGCMC 1 cut(s) 870
BalI TGGCCA 1 cut(s) 390
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 989
Bbv12I GWGCWC 2 cut(s) 66, 870
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 2 cut(s) 120, 336
BccI CCATC 1 cut(s) 296
BcgI CGANNNNNNTGC 4 cut(s) 91, 125, 331, 365
BciT130I CCWGG 2 cut(s) 68, 148
BcnI CCSGG 2 cut(s) 49, 88
BcuI ACTAGT 2 cut(s) 803, 862
BfaI CTAG 3 cut(s) 804, 863, 1122
BfmI CTRYAG 2 cut(s) 350, 786
BfoI RGCGCY 1 cut(s) 350
BisI GCNGC 4 cut(s) 15, 134, 335, 350
BlsI GCNGC 4 cut(s) 16, 135, 336, 351
Bme1390I CCNGG 4 cut(s) 49, 68, 88, 148
Bme18I GGWCC 2 cut(s) 121, 988
BmgT120I GGNCC 3 cut(s) 70, 121, 988
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 4 cut(s) 49, 68, 88, 148
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 5 cut(s) 252, 297, 588, 712, 935
BmtI GCTAGC 1 cut(s) 1125
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 1 cut(s) 989
BpmI CTGGAG 3 cut(s) 50, 287, 341
Bpu10I CCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 49, 88
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 612
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 397, 1134
Bse1I ACTGG 1 cut(s) 1062
BseAI TCCGGA 1 cut(s) 612
BseBI CCWGG 2 cut(s) 68, 148
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 4 cut(s) 307, 312, 610, 727
BseLI CCNNNNNNNGG 3 cut(s) 112, 397, 1134
BseMII CTCAG 3 cut(s) 146, 398, 843
BseNI ACTGG 1 cut(s) 1062
BseRI GAGGAG 6 cut(s) 296, 338, 380, 489, 492, 495
BseSI GKGCMC 1 cut(s) 870
BseXI GCAGC 2 cut(s) 120, 336
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 2 cut(s) 14, 547
BshFI GGCC 3 cut(s) 71, 390, 1105
BsiHKAI GWGCWC 2 cut(s) 66, 870
BsiSI CCGG 3 cut(s) 49, 88, 613
BslI CCNNNNNNNGG 3 cut(s) 112, 397, 1134
BsmI GAATGC 4 cut(s) 114, 337, 514, 916
BsnI GGCC 3 cut(s) 71, 390, 1105
Bsp119I TTCGAA 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 66, 870
Bsp13I TCCGGA 1 cut(s) 612
Bsp143I GATC 2 cut(s) 9, 793
BspACI CCGC 7 cut(s) 14, 17, 73, 173, 334, 496, 547
BspANI GGCC 3 cut(s) 71, 390, 1105
BspCNI CTCAG 3 cut(s) 147, 399, 844
BspEI TCCGGA 1 cut(s) 612
BspFNI CGCG 2 cut(s) 14, 547
BspLI GGNNCC 1 cut(s) 92
BspMAI CTGCAG 1 cut(s) 354
BspOI GCTAGC 1 cut(s) 1125
BspPI GGATC 1 cut(s) 17
BspQI GCTCTTC 1 cut(s) 243
BspT104I TTCGAA 1 cut(s) 341
BsrI ACTGG 1 cut(s) 1062
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 2 cut(s) 9, 793
Bst2UI CCWGG 2 cut(s) 68, 148
Bst4CI ACNGT 2 cut(s) 181, 849
Bst6I CTCTTC 2 cut(s) 243, 414
BstBI TTCGAA 1 cut(s) 341
BstC8I GCNNGC 4 cut(s) 73, 114, 1107, 1123
BstDEI CTNAG 4 cut(s) 155, 407, 809, 852
BstF5I GGATG 4 cut(s) 307, 312, 610, 727
BstFNI CGCG 2 cut(s) 14, 547
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 2 cut(s) 12, 796
BstMBI GATC 2 cut(s) 9, 793
BstMWI GCNNNNNNNGC 5 cut(s) 139, 316, 325, 334, 1077
BstNI CCWGG 2 cut(s) 68, 148
BstSCI CCNGG 4 cut(s) 47, 66, 86, 146
BstSFI CTRYAG 2 cut(s) 350, 786
BstSLI GKGCMC 1 cut(s) 870
BstUI CGCG 2 cut(s) 14, 547
BstV1I GCAGC 2 cut(s) 120, 336
BstV2I GAAGAC 1 cut(s) 989
BsuRI GGCC 3 cut(s) 71, 390, 1105
BtgZI GCGATG 1 cut(s) 1061
BtsCI GGATG 4 cut(s) 307, 312, 610, 727
Cac8I GCNNGC 4 cut(s) 73, 114, 1107, 1123
CaiI CAGNNNCTG 1 cut(s) 118
CfoI GCGC 1 cut(s) 349
Cfr13I GGNCC 3 cut(s) 70, 121, 988
Csp6I GTAC 1 cut(s) 695
CviAII CATG 1 cut(s) 1129
CviQI GTAC 1 cut(s) 695
DdeI CTNAG 4 cut(s) 155, 407, 809, 852
DpnI GATC 2 cut(s) 11, 795
DpnII GATC 2 cut(s) 9, 793
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 2 cut(s) 388, 1103
Eam1104I CTCTTC 2 cut(s) 243, 414
EarI CTCTTC 2 cut(s) 243, 414
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 2 cut(s) 121, 988
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 66, 146
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 1 cut(s) 1132
FatI CATG 1 cut(s) 1128
FauI CCCGC 2 cut(s) 80, 489
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 15, 134, 335, 350
FokI GGATG 4 cut(s) 314, 319, 617, 734
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 4 cut(s) 15, 134, 335, 350
FspBI CTAG 3 cut(s) 804, 863, 1122
GlaI GCGC 1 cut(s) 348
GluI GCNGC 4 cut(s) 15, 134, 335, 350
GsuI CTGGAG 3 cut(s) 50, 287, 341
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 3 cut(s) 71, 390, 1105
HapII CCGG 3 cut(s) 49, 88, 613
HhaI GCGC 1 cut(s) 349
Hin1II CATG 1 cut(s) 1132
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 2 cut(s) 40, 57
HindII GTYRAC 2 cut(s) 40, 57
HindIII AAGCTT 2 cut(s) 527, 834
HinfI GANTC 4 cut(s) 80, 314, 616, 1132
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 3 cut(s) 49, 88, 613
HphI GGTGA 5 cut(s) 172, 271, 412, 473, 667
Hpy166II GTNNAC 5 cut(s) 40, 57, 675, 738, 868
Hpy188I TCNGA 5 cut(s) 169, 185, 756, 853, 1137
Hpy188III TCNNGA 3 cut(s) 100, 119, 613
Hpy8I GTNNAC 5 cut(s) 40, 57, 675, 738, 868
HpyAV CCTTC 5 cut(s) 337, 418, 439, 676, 769
HpyCH4III ACNGT 2 cut(s) 181, 849
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 316, 325, 334, 1077
HpyF3I CTNAG 4 cut(s) 155, 407, 809, 852
Hsp92II CATG 1 cut(s) 1132
HspAI GCGC 1 cut(s) 347
Kpn2I TCCGGA 1 cut(s) 612
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 2 cut(s) 9, 793
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 8 cut(s) 61, 69, 102, 130, 151, 220, 325, 889
Lsp1109I GCAGC 2 cut(s) 120, 336
LweI GCATC 5 cut(s) 252, 297, 588, 712, 935
MaeI CTAG 3 cut(s) 804, 863, 1122
MaeIII GTNAC 1 cut(s) 21
MalI GATC 2 cut(s) 11, 795
MboI GATC 2 cut(s) 9, 793
MboII GAAGA 5 cut(s) 260, 431, 769, 989, 1052
MfeI CAATTG 1 cut(s) 137
MhlI GDGCHC 2 cut(s) 66, 870
MlsI TGGCCA 1 cut(s) 390
MluCI AATT 3 cut(s) 137, 371, 1095
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 3 cut(s) 74, 323, 1126
MmeI TCCRAC 4 cut(s) 147, 219, 418, 631
Mox20I TGGCCA 1 cut(s) 390
MroI TCCGGA 1 cut(s) 612
MscI TGGCCA 1 cut(s) 390
MseI TTAA 3 cut(s) 56, 704, 1098
MslI CAYNNNNRTG 1 cut(s) 941
Msp20I TGGCCA 1 cut(s) 390
MspI CCGG 3 cut(s) 49, 88, 613
MspR9I CCNGG 4 cut(s) 49, 68, 88, 148
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 4 cut(s) 114, 337, 514, 916
MvaI CCWGG 2 cut(s) 68, 148
MvnI CGCG 2 cut(s) 14, 547
MwoI GCNNNNNNNGC 5 cut(s) 139, 316, 325, 334, 1077
NciI CCSGG 2 cut(s) 49, 88
NdeII GATC 2 cut(s) 9, 793
NheI GCTAGC 1 cut(s) 1121
NlaIII CATG 1 cut(s) 1132
NlaIV GGNNCC 1 cut(s) 92
NspV TTCGAA 1 cut(s) 341
PciSI GCTCTTC 1 cut(s) 243
PctI GAATGC 4 cut(s) 114, 337, 514, 916
PfeI GAWTC 1 cut(s) 616
PkrI GCNGC 4 cut(s) 16, 135, 336, 351
PleI GAGTC 3 cut(s) 74, 322, 1126
PpsI GAGTC 3 cut(s) 74, 322, 1126
PshBI ATTAAT 1 cut(s) 1098
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 66, 146
PspGI CCWGG 2 cut(s) 66, 146
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 3 cut(s) 70, 121, 988
PstI CTGCAG 1 cut(s) 354
PstNI CAGNNNCTG 1 cut(s) 118
RsaI GTAC 1 cut(s) 696
RsaNI GTAC 1 cut(s) 695
RseI CAYNNNNRTG 1 cut(s) 941
SacI GAGCTC 1 cut(s) 66
SalI GTCGAC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 56, 704, 1098
SatI GCNGC 4 cut(s) 15, 134, 335, 350
Sau3AI GATC 2 cut(s) 9, 793
Sau96I GGNCC 3 cut(s) 70, 121, 988
SchI GAGTC 3 cut(s) 74, 323, 1126
ScrFI CCNGG 4 cut(s) 49, 68, 88, 148
SduI GDGCHC 2 cut(s) 66, 870
SfaNI GCATC 5 cut(s) 252, 297, 588, 712, 935
SfcI CTRYAG 2 cut(s) 350, 786
SfuI TTCGAA 1 cut(s) 341
SinI GGWCC 2 cut(s) 121, 988
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SpeI ACTAGT 2 cut(s) 803, 862
Sse9I AATT 3 cut(s) 137, 371, 1095
SsiI CCGC 7 cut(s) 14, 17, 73, 173, 334, 496, 547
SspMI CTAG 3 cut(s) 804, 863, 1122
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 4 cut(s) 47, 66, 86, 146
TaaI ACNGT 2 cut(s) 181, 849
TaqI TCGA 4 cut(s) 39, 83, 341, 796
TasI AATT 3 cut(s) 137, 371, 1095
TauI GCSGC 2 cut(s) 17, 337
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 3 cut(s) 56, 704, 1098
Tru9I TTAA 3 cut(s) 56, 704, 1098
TseI GCWGC 2 cut(s) 133, 349
TspDTI ATGAA 1 cut(s) 959
TspGWI ACGGA 2 cut(s) 74, 990
VneI GTGCAC 1 cut(s) 866
VpaK11BI GGWCC 2 cut(s) 121, 988
VspI ATTAAT 1 cut(s) 1098
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 3 cut(s) 804, 863, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.