Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0009021

Physical Location & Seq

Genomic Coordinates Forward (+)

3365460 .. 3371811

Gene Structure 6352 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g05910.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2094 bp

ATGGAATCGGATGCAGCTCTACGTCGCCTCCACGCCGTACAATCCCACCTCCTCCCTCCCACGGCCGACGATCCTCAACCCCTCCTTCAGAACAACCTCGCCGCCGGCGAGTTTGCTCACGGGAAGGGGTACAGTGTAGTACTCCCGGAGAAGTTGCAAACAGGAAAATGGAATGTGTACAGATCCGTACGTTCCCCTTTCGAGCTTGTAACTCGGTTTGCTGATCATCCGGATATTGTCACATTGCACGATAACTTTGTACGTTCGGCTGAGACTTTTAAAGATCACAAGTATTTGGGTACACGAATTCGGAGTGATGGAACTGTTGGAGAGTACAAATGGATGACATACGGTGAAGCAGCAACAGTTCGGGAAGCATTAGGTTCCGGCCTGTGGTTAAATGGGATACAAAAGGGAGCGTGTGTGGGACTCTTTTTCATAAACAGACCAGAGTGGTTGATTGTGGATCATGCTTGTTCAGCATATTCTTTTATCTCAGTTCCTCTATACGATACTCTTGGACCTGATGCGGTTAAGTATGTCGTGAATCATGCCAATGTACAAGCAATTTTTTGTTCTCCCGATAATATGAACACTTTGCTGAGCTTACTTTCCGAGATCCAATCTGTAGCTCTTATAGTGGTAGTGGGAGGGGTAGATGAACACTTGCGTTCCCTTCCATCTATAACTGGAGTGAAACTCGTGTCATATATGGAGCTCGTCAGTCAGGGCCGCAGTTGCCTGCAGCCTTTTTGCCCTCCTAATTCTGAAGATGTTGCGACCATATGTTATACAAGTGGTACAACTGGAACACCAAAGGGTGTTGTGTTGACTCATGGAAACTTAATATCAAGTTGTGCTGGATTCAGTTGTGCATTGCGATTCTGTTCATCTGATATTTACATATCCTATCTTCCTTTGGCACATATATACGAGCGAGCTAATCAAATTGTCGTACTGTACTTTGGTGGAGCTTGTGGCTTCTACCAGGGGGACAATCTGAAATTGATGGATGACCTGGCTGTATTAAGACCAACACTATTTTGCAGTGTTCCACGGGTGTACAATCGAATCTATGCTGGAATCAACAACGCTGTTAAAACTTCTGGGATTCTAAAGGAGAGACTTTTCAGAGCTGCTTACAATTCTAAGAAGCAATCCATAATGAGTGGTTCAACATCATCACCAATATGGGACAGATTGGTATTTAATAAAATCAAGGCAAAGCTTGGAGGACGAGTCCGTTTTATGTCATCTGGTGCTTCACCCTTGTCTCCTGATGTCATGGACTTCCTTAGGGTGTGCTTTGGCTGTCAAGTAATTGAAGGATATGGTATGACAGAGACTACTTCTGTCATATGCATGATGGATGAGGGTGACACCTTAAGTGGCCATGTTGGTTCCCCTAGTGCAGCTTGTGAAGTAAAACTAGTCGATGTCCCTGAAATGAATTACACCTCTGACGACCAGCCATATCCTCGTGGGGAAATTTGTGTCCGCGGTCCCATTGTCTTCCAAGGCTACTACAAAGACGAACTGCAGACCAAAGAAGTATTTGATGATGAAGGTTGGCTGCATACTGGAGACATTGGTTTGTGGTTACCTGGAGGCAGGCTTAAGATTATTGATAGGAAAAAGAATATCTTCAAGTTGGCACAGGGTGAGTATGTAGCACCGGAGAAGATAGAGAATGTCTATGCCAAGTGCCAATTTGTTGCTCAGTGTTTTATTTATGGTGATAGCTTTAAGTCCTGTCTAGTAGCTATAGTTGCAGTGGATCCTGATGTATTGAAAGAGTGGGCTGCATCTGAAGGCATAAAGTACGAAGATCTCAGACAATTGTGCAATAATCCAAGAGTAAGGTCTGTAGTTTTGGCTGACATGGATGCCATTGGAAAGGAGGCTCAGTTGAGAGGTTTTGAATTTGCAAAAGCTGTAACTTTAGTTCCTGAACCATTCACAGTAGAGAATGGCCTTCTTACTCCAACCTTTAAGATCAAGAGGCCTCAAGCCAGGGCACACTTTGCGAAAGCAATATCAAACATGTACTCTGAGCTTTCCGTACCCGATATTGTAACGGAAAAAATTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010193 GO:0015645 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0032787 GO:0042221 GO:0042493 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0050896 GO:0071704 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

698

Amino Acids

76.9

Weight (kDa)

6.3

Isoelectric Point (pI)

38.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	91 - 509	1e-89	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27600
fragaria_vesca	FvH4_6g05910 FvH4_6g05910 FvH4_6g05910
prunus_persica	Prupe.3G211300_v2.0.a1 Prupe.6G325200_v2.0.a1 Prupe.6G325200_v2.0.a1 Prupe.6G325200_v2.0.a1 Prupe.6G325200_v2.0.a1 Prupe.8G000300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0454921
rosa_multiflora	Rmu_sc0001792.1_g000009 Rmu_sc0002169.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424680
rosa_rugosa	Rorug03G0002900 Rorug03G0003000
rosa_samantha	Rh3AG062800 Rh3BG064700 Rh3CG063600 Rh3DG064700 Rh6AG155800
rosa_wichuraiana	Rw3G004910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	1500
AccIII	TCCGGA	1 cut(s)	229
AciI	CCGC	5 cut(s)	102, 530, 733, 1498, 1500
AclWI	GGATC	6 cut(s)	65, 177, 474, 613, 1772, 1785
AcoI	YGGCCR	2 cut(s)	63, 1390
AcsI	RAATTY	3 cut(s)	306, 1488, 1922
AcuI	CTGAAG	3 cut(s)	71, 789, 1830
AdeI	CACNNNGTG	1 cut(s)	1661
AfiI	CCNNNNNNNGG	2 cut(s)	61, 393
AflII	CTTAAG	2 cut(s)	1384, 1616
AflIII	ACRYGT	1 cut(s)	2043
AgsI	TTSAA	5 cut(s)	1176, 1325, 1648, 1792, 1922
AhlI	ACTAGT	1 cut(s)	1429
AjnI	CCWGG	4 cut(s)	987, 1017, 1603, 2012
Alw21I	GWGCWC	1 cut(s)	720
Alw26I	GTCTC	5 cut(s)	266, 1117, 1278, 1337, 1578
AlwI	GGATC	6 cut(s)	65, 177, 474, 613, 1772, 1785
Aor13HI	TCCGGA	1 cut(s)	229
AoxI	GGCC	6 cut(s)	63, 388, 730, 1390, 1972, 2003
ApeKI	GCWGC	7 cut(s)	14, 359, 745, 1136, 1412, 1573, 1802
ApoI	RAATTY	3 cut(s)	306, 1488, 1922
Asp700I	GAANNNNTTC	1 cut(s)	1643
AspS9I	GGNCC	3 cut(s)	521, 730, 1502
AsuC2I	CCSGG	1 cut(s)	146
AsuHPI	GGTGA	6 cut(s)	365, 1176, 1257, 1388, 1673, 1748
AvaII	GGWCC	2 cut(s)	521, 1502
AxyI	CCTNAGG	1 cut(s)	1295
BaeGI	GKGCMC	1 cut(s)	2020
BalI	TGGCCA	1 cut(s)	1392
BamHI	GGATCC	1 cut(s)	1777
BanII	GRGCYC	1 cut(s)	720
BauI	CACGAG	2 cut(s)	701, 1479
BbsI	GAAGAC	1 cut(s)	1504
Bbv12I	GWGCWC	1 cut(s)	720
BbvI	GCAGC	7 cut(s)	26, 371, 757, 1123, 1424, 1560, 1789
BccI	CCATC	4 cut(s)	311, 688, 1003, 1360
BceAI	ACGGC	2 cut(s)	20, 78
BciT130I	CCWGG	4 cut(s)	989, 1019, 1605, 2014
BciVI	GTATCC	1 cut(s)	399
BclI	TGATCA	1 cut(s)	223
BcnI	CCSGG	1 cut(s)	146
BcoDI	GTCTC	5 cut(s)	266, 1117, 1278, 1337, 1578
BcuI	ACTAGT	1 cut(s)	1429
BfaI	CTAG	3 cut(s)	1407, 1430, 1757
BfmI	CTRYAG	5 cut(s)	627, 743, 1538, 1764, 1866
BfrI	CTTAAG	2 cut(s)	1384, 1616
BfuI	GTATCC	1 cut(s)	399
BglII	AGATCT	1 cut(s)	1828
BisI	GCNGC	9 cut(s)	15, 102, 360, 733, 746, 1137, 1413, 1574, 1803
BlpI	GCTNAGC	1 cut(s)	602
BlsI	GCNGC	9 cut(s)	16, 103, 361, 734, 747, 1138, 1414, 1575, 1804
BmcAI	AGTACT	1 cut(s)	141
Bme1390I	CCNGG	5 cut(s)	146, 989, 1019, 1605, 2014
Bme18I	GGWCC	2 cut(s)	521, 1502
BmgT120I	GGNCC	3 cut(s)	521, 730, 1502
BmiI	GGNNCC	4 cut(s)	385, 1402, 1504, 1779
BmrFI	CCNGG	5 cut(s)	146, 989, 1019, 1605, 2014
BmsI	GCATC	3 cut(s)	517, 1814, 1876
BpiI	GAAGAC	1 cut(s)	1504
BplI	GAGNNNNNCTC	2 cut(s)	684, 716
BpmI	CTGGAG	3 cut(s)	711, 1602, 1626
Bpu1102I	GCTNAGC	1 cut(s)	602
BpuEI	CTTGAG	1 cut(s)	1992
BpuMI	CCSGG	1 cut(s)	146
BsaJI	CCNNGG	6 cut(s)	60, 988, 1055, 1498, 1516, 2013
BsaWI	WCCGGW	2 cut(s)	229, 1675
BsaXI	ACNNNNNCTCC	8 cut(s)	12, 42, 408, 438, 1224, 1254, 1892, 1922
Bsc4I	CCNNNNNNNGG	2 cut(s)	61, 393
Bse118I	RCCGGY	1 cut(s)	104
Bse1I	ACTGG	3 cut(s)	694, 811, 1585
Bse21I	CCTNAGG	1 cut(s)	1295
Bse3DI	GCAATG	2 cut(s)	242, 875
BseAI	TCCGGA	1 cut(s)	229
BseBI	CCWGG	4 cut(s)	989, 1019, 1605, 2014
BseDI	CCNNGG	6 cut(s)	60, 988, 1055, 1498, 1516, 2013
BseGI	GGATG	6 cut(s)	16, 226, 348, 1018, 1375, 1891
BseLI	CCNNNNNNNGG	2 cut(s)	61, 393
BseMI	GCAATG	2 cut(s)	242, 875
BseMII	CTCAG	7 cut(s)	261, 510, 593, 1733, 1846, 1919, 2043
BseNI	ACTGG	3 cut(s)	694, 811, 1585
BseRI	GAGGAG	1 cut(s)	41
BseSI	GKGCMC	1 cut(s)	2020
BseX3I	CGGCCG	1 cut(s)	63
BseXI	GCAGC	7 cut(s)	26, 371, 757, 1123, 1424, 1560, 1789
BsgI	GTGCAG	1 cut(s)	1431
Bsh1236I	CGCG	1 cut(s)	1500
Bsh1285I	CGRYCG	1 cut(s)	66
BshFI	GGCC	6 cut(s)	65, 390, 732, 1392, 1974, 2005
BsiEI	CGRYCG	1 cut(s)	66
BsiHKAI	GWGCWC	1 cut(s)	720
BsiSI	CCGG	5 cut(s)	105, 146, 230, 387, 1676
BsiWI	CGTACG	1 cut(s)	187
BslFI	GGGAC	5 cut(s)	441, 1007, 1208, 1424, 1488
BslI	CCNNNNNNNGG	2 cut(s)	61, 393
BsmAI	GTCTC	5 cut(s)	266, 1117, 1278, 1337, 1578
BsmFI	GGGAC	5 cut(s)	441, 1007, 1208, 1424, 1488
BsnI	GGCC	6 cut(s)	65, 390, 732, 1392, 1974, 2005
Bsp1286I	GDGCHC	2 cut(s)	720, 2020
Bsp13I	TCCGGA	1 cut(s)	229
Bsp1407I	TGTACA	3 cut(s)	177, 559, 1062
Bsp143I	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
Bsp1720I	GCTNAGC	1 cut(s)	602
BspACI	CCGC	5 cut(s)	102, 530, 733, 1498, 1500
BspANI	GGCC	6 cut(s)	65, 390, 732, 1392, 1974, 2005
BspCNI	CTCAG	7 cut(s)	262, 509, 594, 1732, 1845, 1918, 2044
BspEI	TCCGGA	1 cut(s)	229
BspFNI	CGCG	1 cut(s)	1500
BspLI	GGNNCC	4 cut(s)	385, 1402, 1504, 1779
BspMAI	CTGCAG	2 cut(s)	747, 1542
BspPI	GGATC	6 cut(s)	65, 177, 474, 613, 1772, 1785
BspTI	CTTAAG	2 cut(s)	1384, 1616
BsrDI	GCAATG	2 cut(s)	242, 875
BsrFI	RCCGGY	1 cut(s)	104
BsrGI	TGTACA	3 cut(s)	177, 559, 1062
BsrI	ACTGG	3 cut(s)	694, 811, 1585
BssAI	RCCGGY	1 cut(s)	104
BssECI	CCNNGG	6 cut(s)	60, 988, 1055, 1498, 1516, 2013
BssMI	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
BssSI	CACGAG	2 cut(s)	701, 1479
BssT1I	CCWWGG	1 cut(s)	1516
Bst2BI	CACGAG	2 cut(s)	701, 1479
Bst2UI	CCWGG	4 cut(s)	989, 1019, 1605, 2014
Bst4CI	ACNGT	6 cut(s)	134, 325, 353, 367, 960, 1963
BstAFI	CTTAAG	2 cut(s)	1384, 1616
BstAPI	GCANNNNNTGC	1 cut(s)	2024
BstAUI	TGTACA	3 cut(s)	177, 559, 1062
BstC8I	GCNNGC	4 cut(s)	106, 743, 939, 1613
BstDEI	CTNAG	9 cut(s)	270, 496, 602, 1149, 1295, 1719, 1832, 1905, 2052
BstDSI	CCRYGG	3 cut(s)	60, 1055, 1498
BstEII	GGTNACC	1 cut(s)	1599
BstF5I	GGATG	6 cut(s)	16, 226, 348, 1018, 1375, 1891
BstFNI	CGCG	1 cut(s)	1500
BstKTI	GATC	9 cut(s)	73, 185, 226, 286, 469, 621, 1780, 1831, 1998
BstMAI	GTCTC	5 cut(s)	266, 1117, 1278, 1337, 1578
BstMBI	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
BstMCI	CGRYCG	1 cut(s)	66
BstMWI	GCNNNNNNNGC	4 cut(s)	479, 738, 1769, 2024
BstNI	CCWGG	4 cut(s)	989, 1019, 1605, 2014
BstNSI	RCATGY	1 cut(s)	2047
BstPI	GGTNACC	1 cut(s)	1599
BstSCI	CCNGG	5 cut(s)	144, 987, 1017, 1603, 2012
BstSFI	CTRYAG	5 cut(s)	627, 743, 1538, 1764, 1866
BstSLI	GKGCMC	1 cut(s)	2020
BstUI	CGCG	1 cut(s)	1500
BstV1I	GCAGC	7 cut(s)	26, 371, 757, 1123, 1424, 1560, 1789
BstV2I	GAAGAC	1 cut(s)	1504
BstX2I	RGATCY	4 cut(s)	182, 618, 1777, 1828
BstYI	RGATCY	4 cut(s)	182, 618, 1777, 1828
BstZI	CGGCCG	1 cut(s)	63
Bsu36I	CCTNAGG	1 cut(s)	1295
BsuI	GTATCC	1 cut(s)	399
BsuRI	GGCC	6 cut(s)	65, 390, 732, 1392, 1974, 2005
BtgI	CCRYGG	3 cut(s)	60, 1055, 1498
BtsCI	GGATG	6 cut(s)	16, 226, 348, 1018, 1375, 1891
BtsI	GCAGTG	2 cut(s)	1054, 1779
BtsIMutI	CAGTG	4 cut(s)	139, 1054, 1727, 1779
Cac8I	GCNNGC	4 cut(s)	106, 743, 939, 1613
Cfr10I	RCCGGY	1 cut(s)	104
Cfr13I	GGNCC	3 cut(s)	521, 730, 1502
Cfr42I	CCGCGG	1 cut(s)	1501
CviAII	CATG	8 cut(s)	470, 551, 836, 1285, 1363, 1394, 1882, 2044
DdeI	CTNAG	9 cut(s)	270, 496, 602, 1149, 1295, 1719, 1832, 1905, 2052
DpnI	GATC	9 cut(s)	72, 184, 225, 285, 468, 620, 1779, 1830, 1997
DpnII	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
DraI	TTTAAA	1 cut(s)	280
DraIII	CACNNNGTG	1 cut(s)	1661
EaeI	YGGCCR	2 cut(s)	63, 1390
EagI	CGGCCG	1 cut(s)	63
Ecl136II	GAGCTC	1 cut(s)	718
EclXI	CGGCCG	1 cut(s)	63
Eco130I	CCWWGG	1 cut(s)	1516
Eco147I	AGGCCT	1 cut(s)	2005
Eco24I	GRGCYC	1 cut(s)	720
Eco47I	GGWCC	2 cut(s)	521, 1502
Eco52I	CGGCCG	1 cut(s)	63
Eco53kI	GAGCTC	1 cut(s)	718
Eco57I	CTGAAG	3 cut(s)	71, 789, 1830
Eco81I	CCTNAGG	1 cut(s)	1295
Eco91I	GGTNACC	1 cut(s)	1599
EcoICRI	GAGCTC	1 cut(s)	718
EcoO65I	GGTNACC	1 cut(s)	1599
EcoRI	GAATTC	1 cut(s)	306
EcoRII	CCWGG	4 cut(s)	987, 1017, 1603, 2012
EcoT14I	CCWWGG	1 cut(s)	1516
EcoT22I	ATGCAT	1 cut(s)	1364
EcoT38I	GRGCYC	1 cut(s)	720
ErhI	CCWWGG	1 cut(s)	1516
FaeI	CATG	8 cut(s)	473, 554, 839, 1288, 1366, 1397, 1885, 2047
FalI	AAGNNNNNCTT	2 cut(s)	1628, 1660
FaqI	GGGAC	5 cut(s)	441, 1007, 1208, 1424, 1488
FatI	CATG	8 cut(s)	469, 550, 835, 1284, 1362, 1393, 1881, 2043
FauNDI	CATATG	2 cut(s)	785, 1358
FbaI	TGATCA	1 cut(s)	223
Fnu4HI	GCNGC	9 cut(s)	15, 102, 360, 733, 746, 1137, 1413, 1574, 1803
FokI	GGATG	6 cut(s)	23, 213, 355, 1025, 1382, 1898
FriOI	GRGCYC	1 cut(s)	720
Fsp4HI	GCNGC	9 cut(s)	15, 102, 360, 733, 746, 1137, 1413, 1574, 1803
FspBI	CTAG	3 cut(s)	1407, 1430, 1757
GluI	GCNGC	9 cut(s)	15, 102, 360, 733, 746, 1137, 1413, 1574, 1803
GsuI	CTGGAG	3 cut(s)	711, 1602, 1626
HaeIII	GGCC	6 cut(s)	65, 390, 732, 1392, 1974, 2005
HapII	CCGG	5 cut(s)	105, 146, 230, 387, 1676
Hin1II	CATG	8 cut(s)	473, 554, 839, 1288, 1366, 1397, 1885, 2047
HincII	GTYRAC	1 cut(s)	831
HindII	GTYRAC	1 cut(s)	831
HindIII	AAGCTT	1 cut(s)	1226
HpaII	CCGG	5 cut(s)	105, 146, 230, 387, 1676
HphI	GGTGA	6 cut(s)	365, 1176, 1257, 1388, 1673, 1748
Hpy166II	GTNNAC	4 cut(s)	178, 302, 831, 1063
Hpy188III	TCNNGA	8 cut(s)	230, 371, 544, 581, 1277, 1781, 1949, 1999
Hpy8I	GTNNAC	4 cut(s)	178, 302, 831, 1063
Hpy99I	CGWCG	2 cut(s)	27, 71
HpyAV	CCTTC	7 cut(s)	95, 118, 686, 1319, 1559, 1805, 1985
HpyCH4III	ACNGT	6 cut(s)	134, 325, 353, 367, 960, 1963
HpyCH4IV	ACGT	3 cut(s)	22, 190, 262
HpyF10VI	GCNNNNNNNGC	4 cut(s)	479, 738, 1769, 2024
HpyF3I	CTNAG	9 cut(s)	270, 496, 602, 1149, 1295, 1719, 1832, 1905, 2052
HpySE526I	ACGT	3 cut(s)	22, 190, 262
Hsp92II	CATG	8 cut(s)	473, 554, 839, 1288, 1366, 1397, 1885, 2047
Kpn2I	TCCGGA	1 cut(s)	229
KroI	GCCGGC	1 cut(s)	104
KroNI	GCCGGC	1 cut(s)	106
Ksp22I	TGATCA	1 cut(s)	223
KspI	CCGCGG	1 cut(s)	1501
Kzo9I	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
LmnI	GCTCC	3 cut(s)	416, 715, 971
Lsp1109I	GCAGC	7 cut(s)	26, 371, 757, 1123, 1424, 1560, 1789
LweI	GCATC	3 cut(s)	517, 1814, 1876
MaeI	CTAG	3 cut(s)	1407, 1430, 1757
MaeII	ACGT	3 cut(s)	22, 190, 262
MaeIII	GTNAC	6 cut(s)	208, 238, 1376, 1599, 1936, 2074
MalI	GATC	9 cut(s)	72, 184, 225, 285, 468, 620, 1779, 1830, 1997
MboI	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
MboII	GAAGA	6 cut(s)	782, 905, 1504, 1636, 1693, 1838
MfeI	CAATTG	1 cut(s)	1838
MflI	RGATCY	4 cut(s)	182, 618, 1777, 1828
MhlI	GDGCHC	2 cut(s)	720, 2020
MlsI	TGGCCA	1 cut(s)	1392
MluNI	TGGCCA	1 cut(s)	1392
MlyI	GAGTC	3 cut(s)	423, 826, 1248
MmeI	TCCRAC	2 cut(s)	307, 2009
Mox20I	TGGCCA	1 cut(s)	1392
Mph1103I	ATGCAT	1 cut(s)	1364
MreI	CGCCGGCG	1 cut(s)	104
MroI	TCCGGA	1 cut(s)	229
MroNI	GCCGGC	1 cut(s)	104
MroXI	GAANNNNTTC	1 cut(s)	1643
MscI	TGGCCA	1 cut(s)	1392
MslI	CAYNNNNRTG	3 cut(s)	555, 1189, 1361
Msp20I	TGGCCA	1 cut(s)	1392
MspA1I	CMGCKG	1 cut(s)	1500
MspCI	CTTAAG	2 cut(s)	1384, 1616
MspI	CCGG	5 cut(s)	105, 146, 230, 387, 1676
MspR9I	CCNGG	5 cut(s)	146, 989, 1019, 1605, 2014
MunI	CAATTG	1 cut(s)	1838
MvaI	CCWGG	4 cut(s)	989, 1019, 1605, 2014
MvnI	CGCG	1 cut(s)	1500
MwoI	GCNNNNNNNGC	4 cut(s)	479, 738, 1769, 2024
NaeI	GCCGGC	1 cut(s)	106
NciI	CCSGG	1 cut(s)	146
NdeI	CATATG	2 cut(s)	785, 1358
NdeII	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
NgoMIV	GCCGGC	1 cut(s)	104
NlaIII	CATG	8 cut(s)	473, 554, 839, 1288, 1366, 1397, 1885, 2047
NlaIV	GGNNCC	4 cut(s)	385, 1402, 1504, 1779
NmuCI	GTSAC	2 cut(s)	238, 1376
NsiI	ATGCAT	1 cut(s)	1364
NspI	RCATGY	1 cut(s)	2047
PceI	AGGCCT	1 cut(s)	2005
PciI	ACATGT	1 cut(s)	2043
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PdiI	GCCGGC	1 cut(s)	106
PdmI	GAANNNNTTC	1 cut(s)	1643
PfeI	GAWTC	7 cut(s)	5, 547, 864, 882, 1071, 1083, 1111
Pfl23II	CGTACG	1 cut(s)	187
PfoI	TCCNGGA	1 cut(s)	144
PkrI	GCNGC	9 cut(s)	16, 103, 361, 734, 747, 1138, 1414, 1575, 1804
PleI	GAGTC	3 cut(s)	423, 826, 1247
PpsI	GAGTC	3 cut(s)	423, 826, 1247
PscI	ACATGT	1 cut(s)	2043
Psp124BI	GAGCTC	1 cut(s)	720
Psp6I	CCWGG	4 cut(s)	987, 1017, 1603, 2012
PspEI	GGTNACC	1 cut(s)	1599
PspGI	CCWGG	4 cut(s)	987, 1017, 1603, 2012
PspLI	CGTACG	1 cut(s)	187
PspN4I	GGNNCC	4 cut(s)	385, 1402, 1504, 1779
PspPI	GGNCC	3 cut(s)	521, 730, 1502
PsrI	GAACNNNNNNTAC	2 cut(s)	1929, 1961
PstI	CTGCAG	2 cut(s)	747, 1542
PsuI	RGATCY	4 cut(s)	182, 618, 1777, 1828
RseI	CAYNNNNRTG	3 cut(s)	555, 1189, 1361
SacI	GAGCTC	1 cut(s)	720
SacII	CCGCGG	1 cut(s)	1501
SatI	GCNGC	9 cut(s)	15, 102, 360, 733, 746, 1137, 1413, 1574, 1803
Sau3AI	GATC	9 cut(s)	70, 182, 223, 283, 466, 618, 1777, 1828, 1995
Sau96I	GGNCC	3 cut(s)	521, 730, 1502
ScaI	AGTACT	1 cut(s)	141
SchI	GAGTC	3 cut(s)	423, 826, 1248
ScrFI	CCNGG	5 cut(s)	146, 989, 1019, 1605, 2014
SduI	GDGCHC	2 cut(s)	720, 2020
SfaNI	GCATC	3 cut(s)	517, 1814, 1876
SfcI	CTRYAG	5 cut(s)	627, 743, 1538, 1764, 1866
Sfr303I	CCGCGG	1 cut(s)	1501
SgrAI	CRCCGGYG	1 cut(s)	104
SgrBI	CCGCGG	1 cut(s)	1501
SinI	GGWCC	2 cut(s)	521, 1502
SmiMI	CAYNNNNRTG	3 cut(s)	555, 1189, 1361
SmlI	CTYRAG	3 cut(s)	1384, 1616, 2007
SmoI	CTYRAG	3 cut(s)	1384, 1616, 2007
SpeI	ACTAGT	1 cut(s)	1429
SseBI	AGGCCT	1 cut(s)	2005
SsiI	CCGC	5 cut(s)	102, 530, 733, 1498, 1500
SspMI	CTAG	3 cut(s)	1407, 1430, 1757
SstI	GAGCTC	1 cut(s)	720
StuI	AGGCCT	1 cut(s)	2005
StyD4I	CCNGG	5 cut(s)	144, 987, 1017, 1603, 2012
StyI	CCWWGG	1 cut(s)	1516
TaaI	ACNGT	6 cut(s)	134, 325, 353, 367, 960, 1963
TaiI	ACGT	3 cut(s)	25, 193, 265
TaqI	TCGA	3 cut(s)	201, 1069, 1434
TatI	WGTACW	7 cut(s)	139, 177, 333, 559, 960, 1062, 2046
TauI	GCSGC	2 cut(s)	104, 735
TfiI	GAWTC	7 cut(s)	5, 547, 864, 882, 1071, 1083, 1111
TscAI	CASTG	4 cut(s)	139, 1054, 1727, 1779
TseFI	GTSAC	2 cut(s)	238, 1376
TseI	GCWGC	7 cut(s)	14, 359, 745, 1136, 1412, 1573, 1802
Tsp45I	GTSAC	2 cut(s)	238, 1376
TspDTI	ATGAA	6 cut(s)	427, 605, 675, 879, 1463, 1578
TspGWI	ACGGA	4 cut(s)	175, 1232, 2050, 2093
TspRI	CASTG	4 cut(s)	139, 1054, 1727, 1779
Vha464I	CTTAAG	2 cut(s)	1384, 1616
VpaK11BI	GGWCC	2 cut(s)	521, 1502
XapI	RAATTY	3 cut(s)	306, 1488, 1922
XceI	RCATGY	1 cut(s)	2047
XmnI	GAANNNNTTC	1 cut(s)	1643
XspI	CTAG	3 cut(s)	1407, 1430, 1757
ZrmI	AGTACT	1 cut(s)	141
Zsp2I	ATGCAT	1 cut(s)	1364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.