FvH4_6g15632

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015784
Physical Location & Seq
Reverse (-)
9746616 .. 9748486
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15632.t2

Sequence Viewer

Length: 921 bp
ATGCAGATCTTCGTGAAAACCCTGACCGGCAAGACCATCACCCTCGAGGTTGAGAGCTCCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAGGGCATCCCTCCCGACCAGCAGAGGCTCATCTTCGCCGGAAAGCAGCTCGAGGACGGCCGGACTTTGGCCGACTACAACATCCAGAAGGAGTCAACTCTCCACCTGGTGCTCCGTCTCAGGGGTGGTATGCAGATCTTCGTGAAAACCCTAACCGGAAAGACGATCACTCTCGAGGTCGAGAGCTCCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAAGGGATCCCCCCAGATCAGCAGAGGTTGATCTTCGCCGGAAAGCAGCTCGAGGACGGTAGGACCTTGGCTGATTACAACATCCAGAAGGAGTCAACCCTCCATTTGGTGCTCCGTCTTCGCGGTGGGATGCAGATCTTCGTGAAGACCCTTACCGGGAAGACCATCACTCTCGAGGTTGAGAGCTCCGACACGATTGACAACGTCAAGGCTAAGATCCAGGATAAGGAGGGAATTCCCCCGGATCAGCAGAGATTGATCTTTGCCGGTAAGCAGTTGGAGGACGGCAGGACCCTGGCCGATTACAATATCCAGAAGGAGTCGACCCTTCACTTGGTGCTCCGATTGAGGGGTGGTATGCAGATTTTTGTGAAGACTTTGACAGGGAAGACCATTACCCTGGAGGTTGAGAGCTCCGATACAATTGATAACGTCAAGGCCAAGATCCAGGATAAGGAAGGGATCCCACCGGATCAGCAGAGGTTGATCTTTGCAGGGAAGCAGCTCGAGGATGGTCGTACCCTGGCTGATTACAATATCCAGAAGGAGTCCACCCTTCACTTGGTCCTCCGTCTCCGTGGTGGTGACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

307

Amino Acids

34.31

Weight (kDa)

6.48

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 3.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 4.4e-32 Ubiquitin family
Rad60-SLD PF11976 77 - 147 3.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 4.4e-32 Ubiquitin family
Rad60-SLD PF11976 153 - 223 3.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 4.4e-32 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 44
AccI GTMKAC 1 cut(s) 650
AccII CGCG 1 cut(s) 450
AciI CCGC 1 cut(s) 450
AcoI YGGCCR 3 cut(s) 157, 168, 624
AcsI RAATTY 1 cut(s) 561
AdeI CACNNNGTG 2 cut(s) 208, 664
AfaI GTAC 1 cut(s) 847
AjnI CCWGG 8 cut(s) 90, 204, 318, 546, 621, 726, 774, 849
AluBI AGCT 7 cut(s) 57, 148, 285, 376, 513, 741, 832
AluI AGCT 7 cut(s) 57, 148, 285, 376, 513, 741, 832
Alw21I GWGCWC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
Alw26I GTCTC 2 cut(s) 221, 905
Ama87I CYCGRG 6 cut(s) 44, 149, 272, 377, 500, 833
AoxI GGCC 6 cut(s) 81, 157, 168, 309, 624, 765
ApeKI GCWGC 3 cut(s) 145, 373, 829
ApoI RAATTY 1 cut(s) 561
AspS9I GGNCC 3 cut(s) 390, 618, 892
AsuC2I CCSGG 2 cut(s) 484, 569
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 6 cut(s) 44, 149, 272, 377, 500, 833
AvaII GGWCC 3 cut(s) 390, 618, 892
BamHI GGATCC 2 cut(s) 333, 789
BanII GRGCYC 4 cut(s) 59, 287, 515, 743
BbsI GAAGAC 5 cut(s) 437, 479, 494, 707, 722
Bbv12I GWGCWC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
BbvI GCAGC 3 cut(s) 157, 385, 841
BccI CCATC 5 cut(s) 44, 74, 302, 500, 833
BceAI ACGGC 2 cut(s) 172, 628
BciT130I CCWGG 8 cut(s) 92, 206, 320, 548, 623, 728, 776, 851
BcnI CCSGG 2 cut(s) 484, 569
BcoDI GTCTC 2 cut(s) 221, 905
BglII AGATCT 3 cut(s) 6, 234, 462
BisI GCNGC 3 cut(s) 146, 374, 830
BlsI GCNGC 3 cut(s) 147, 375, 831
Bme18I GGWCC 3 cut(s) 390, 618, 892
BmeT110I CYCGRG 6 cut(s) 44, 149, 272, 377, 500, 833
BmgT120I GGNCC 3 cut(s) 390, 618, 892
BmiI GGNNCC 3 cut(s) 335, 620, 791
BmsI GCATC 2 cut(s) 114, 447
BpiI GAAGAC 5 cut(s) 437, 479, 494, 707, 722
BpmI CTGGAG 1 cut(s) 749
BpuMI CCSGG 2 cut(s) 484, 569
BsaBI GATNNNNATC 1 cut(s) 461
BsaJI CCNNGG 6 cut(s) 393, 567, 621, 726, 849, 904
BsaWI WCCGGW 2 cut(s) 254, 796
Bse118I RCCGGY 2 cut(s) 26, 593
Bse8I GATNNNNATC 1 cut(s) 461
BseBI CCWGG 8 cut(s) 92, 206, 320, 548, 623, 728, 776, 851
BseDI CCNNGG 6 cut(s) 393, 567, 621, 726, 849, 904
BseGI GGATG 5 cut(s) 105, 180, 408, 462, 844
BseJI GATNNNNATC 1 cut(s) 461
BseMII CTCAG 1 cut(s) 232
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 3 cut(s) 157, 385, 841
Bsh1236I CGCG 1 cut(s) 450
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 6 cut(s) 83, 159, 170, 311, 626, 767
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
BsiHKCI CYCGRG 6 cut(s) 44, 149, 272, 377, 500, 833
BsiSI CCGG 9 cut(s) 27, 138, 160, 255, 366, 483, 569, 594, 797
BsmAI GTCTC 2 cut(s) 221, 905
BsmBI CGTCTC 2 cut(s) 221, 905
BsnI GGCC 6 cut(s) 83, 159, 170, 311, 626, 767
BsoBI CYCGRG 6 cut(s) 44, 149, 272, 377, 500, 833
Bsp1286I GDGCHC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
BspACI CCGC 1 cut(s) 450
BspANI GGCC 6 cut(s) 83, 159, 170, 311, 626, 767
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 1 cut(s) 450
BspLI GGNNCC 3 cut(s) 335, 620, 791
BsrFI RCCGGY 2 cut(s) 26, 593
BssAI RCCGGY 2 cut(s) 26, 593
BssECI CCNNGG 6 cut(s) 393, 567, 621, 726, 849, 904
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 8 cut(s) 92, 206, 320, 548, 623, 728, 776, 851
Bst4CI ACNGT 1 cut(s) 386
BstDEI CTNAG 2 cut(s) 218, 540
BstDSI CCRYGG 1 cut(s) 904
BstF5I GGATG 5 cut(s) 105, 180, 408, 462, 844
BstFNI CGCG 1 cut(s) 450
BstMAI GTCTC 2 cut(s) 221, 905
BstMCI CGRYCG 1 cut(s) 160
BstNI CCWGG 8 cut(s) 92, 206, 320, 548, 623, 728, 776, 851
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 3 cut(s) 157, 385, 841
BstV2I GAAGAC 5 cut(s) 437, 479, 494, 707, 722
BstX2I RGATCY 9 cut(s) 6, 87, 234, 315, 333, 462, 543, 771, 789
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 9 cut(s) 6, 87, 234, 315, 333, 462, 543, 771, 789
BstZI CGGCCG 1 cut(s) 157
BsuRI GGCC 6 cut(s) 83, 159, 170, 311, 626, 767
BtgI CCRYGG 1 cut(s) 904
BtsCI GGATG 5 cut(s) 105, 180, 408, 462, 844
Cfr10I RCCGGY 2 cut(s) 26, 593
Cfr13I GGNCC 3 cut(s) 390, 618, 892
CsiI ACCWGGT 1 cut(s) 204
Csp6I GTAC 1 cut(s) 846
CviQI GTAC 1 cut(s) 846
DdeI CTNAG 2 cut(s) 218, 540
DraIII CACNNNGTG 2 cut(s) 208, 664
EaeI YGGCCR 3 cut(s) 157, 168, 624
EagI CGGCCG 1 cut(s) 157
Ecl136II GAGCTC 4 cut(s) 57, 285, 513, 741
EclXI CGGCCG 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 4 cut(s) 59, 287, 515, 743
Eco47I GGWCC 3 cut(s) 390, 618, 892
Eco52I CGGCCG 1 cut(s) 157
Eco53kI GAGCTC 4 cut(s) 57, 285, 513, 741
Eco88I CYCGRG 6 cut(s) 44, 149, 272, 377, 500, 833
EcoICRI GAGCTC 4 cut(s) 57, 285, 513, 741
EcoO109I RGGNCCY 2 cut(s) 390, 618
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 8 cut(s) 90, 204, 318, 546, 621, 726, 774, 849
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 4 cut(s) 59, 287, 515, 743
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 2 cut(s) 221, 905
FaiI YATR 2 cut(s) 230, 686
FblI GTMKAC 1 cut(s) 650
Fnu4HI GCNGC 3 cut(s) 146, 374, 830
FokI GGATG 5 cut(s) 92, 167, 395, 469, 851
FriOI GRGCYC 4 cut(s) 59, 287, 515, 743
Fsp4HI GCNGC 3 cut(s) 146, 374, 830
GluI GCNGC 3 cut(s) 146, 374, 830
GsuI CTGGAG 1 cut(s) 749
HaeIII GGCC 6 cut(s) 83, 159, 170, 311, 626, 767
HapII CCGG 9 cut(s) 27, 138, 160, 255, 366, 483, 569, 594, 797
HincII GTYRAC 3 cut(s) 195, 423, 651
HindII GTYRAC 3 cut(s) 195, 423, 651
HinfI GANTC 4 cut(s) 191, 419, 647, 875
HpaII CCGG 9 cut(s) 27, 138, 160, 255, 366, 483, 569, 594, 797
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 4 cut(s) 195, 423, 651, 879
Hpy188I TCNGA 5 cut(s) 61, 289, 517, 671, 745
Hpy8I GTNNAC 4 cut(s) 195, 423, 651, 879
HpyAV CCTTC 8 cut(s) 181, 323, 409, 637, 665, 779, 865, 893
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 4 cut(s) 75, 303, 531, 759
HpyCH4V TGCA 5 cut(s) 4, 232, 460, 688, 821
HpyF3I CTNAG 2 cut(s) 218, 540
HpySE526I ACGT 4 cut(s) 75, 303, 531, 759
LmnI GCTCC 7 cut(s) 62, 216, 290, 444, 518, 672, 746
Lsp1109I GCAGC 3 cut(s) 157, 385, 841
LweI GCATC 2 cut(s) 114, 447
MabI ACCWGGT 1 cut(s) 204
MaeII ACGT 4 cut(s) 75, 303, 531, 759
MaeIII GTNAC 1 cut(s) 911
MboII GAAGA 9 cut(s) 124, 229, 352, 437, 457, 484, 499, 712, 727
MfeI CAATTG 1 cut(s) 750
MflI RGATCY 9 cut(s) 6, 87, 234, 315, 333, 462, 543, 771, 789
MhlI GDGCHC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
MluCI AATT 2 cut(s) 561, 750
MlyI GAGTC 4 cut(s) 200, 428, 656, 884
MmeI TCCRAC 4 cut(s) 84, 312, 540, 585
MseI TTAA 1 cut(s) 919
MspI CCGG 9 cut(s) 27, 138, 160, 255, 366, 483, 569, 594, 797
MunI CAATTG 1 cut(s) 750
MvaI CCWGG 8 cut(s) 92, 206, 320, 548, 623, 728, 776, 851
MvnI CGCG 1 cut(s) 450
NciI CCSGG 2 cut(s) 484, 569
NlaIV GGNNCC 3 cut(s) 335, 620, 791
NmuCI GTSAC 1 cut(s) 911
PaeR7I CTCGAG 6 cut(s) 44, 149, 272, 377, 500, 833
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PflFI GACNNNGTC 3 cut(s) 74, 302, 530
PfoI TCCNGGA 4 cut(s) 90, 318, 546, 774
PkrI GCNGC 3 cut(s) 147, 375, 831
PleI GAGTC 4 cut(s) 199, 427, 655, 883
PpsI GAGTC 4 cut(s) 199, 427, 655, 883
PpuMI RGGWCCY 2 cut(s) 390, 618
Psp124BI GAGCTC 4 cut(s) 59, 287, 515, 743
Psp5II RGGWCCY 2 cut(s) 390, 618
Psp6I CCWGG 8 cut(s) 90, 204, 318, 546, 621, 726, 774, 849
PspGI CCWGG 8 cut(s) 90, 204, 318, 546, 621, 726, 774, 849
PspN4I GGNNCC 3 cut(s) 335, 620, 791
PspPI GGNCC 3 cut(s) 390, 618, 892
PspPPI RGGWCCY 2 cut(s) 390, 618
PspXI VCTCGAGB 4 cut(s) 44, 149, 377, 833
PsuI RGATCY 9 cut(s) 6, 87, 234, 315, 333, 462, 543, 771, 789
PsyI GACNNNGTC 3 cut(s) 74, 302, 530
RsaI GTAC 1 cut(s) 847
RsaNI GTAC 1 cut(s) 846
SacI GAGCTC 4 cut(s) 59, 287, 515, 743
SalI GTCGAC 1 cut(s) 649
SaqAI TTAA 1 cut(s) 919
SatI GCNGC 3 cut(s) 146, 374, 830
Sau96I GGNCC 3 cut(s) 390, 618, 892
SchI GAGTC 4 cut(s) 200, 428, 656, 884
SduI GDGCHC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 2 cut(s) 114, 447
Sfr274I CTCGAG 6 cut(s) 44, 149, 272, 377, 500, 833
SinI GGWCC 3 cut(s) 390, 618, 892
SlaI CTCGAG 6 cut(s) 44, 149, 272, 377, 500, 833
SmlI CTYRAG 6 cut(s) 44, 149, 272, 377, 500, 833
SmoI CTYRAG 6 cut(s) 44, 149, 272, 377, 500, 833
Sse9I AATT 2 cut(s) 561, 750
SsiI CCGC 1 cut(s) 450
SstI GAGCTC 4 cut(s) 59, 287, 515, 743
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 4 cut(s) 78, 306, 534, 762
TasI AATT 2 cut(s) 561, 750
Tru1I TTAA 1 cut(s) 919
Tru9I TTAA 1 cut(s) 919
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 3 cut(s) 145, 373, 829
Tsp45I GTSAC 1 cut(s) 911
TspGWI ACGGA 4 cut(s) 203, 431, 887, 893
Tth111I GACNNNGTC 3 cut(s) 74, 302, 530
VpaK11BI GGWCC 3 cut(s) 390, 618, 892
XapI RAATTY 1 cut(s) 561
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XhoI CTCGAG 6 cut(s) 44, 149, 272, 377, 500, 833
XmiI GTMKAC 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.