FvH4_6g18500

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014365
Physical Location & Seq
Reverse (-)
12258333 .. 12259153
821 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18500.t1

Sequence Viewer

Length: 654 bp
ATGTCACTCTCTCTTGCCCTTATGTTTGTTAGTTTCATCCTCCTGTGTGCCACAACATTAGCTTCCGATCCCGACCCAGTTCAAGACTATTGCTTACCGAATCTGAAATCCGGTACAATAAGAACACCCCACCACACCATCCTCCCATGCAGGAACTCGTCCGAGGCCATCACCAATGACTTCGTCTTCTCCGGAATGAAGGCACCCGGGAACTTCACCAACATGGGCCTCGCAGCCATCTCGGTGAACCCGACAATCTTTCCAGGGATTAACACACTAGGGATGTCATTTGTAAGAGCTGATCTCAAGGTGGGTGGAATCAATCCACCGCATTTCCACCCAAGAGCCACAGAAATAGCATATATAGTGAAAGGGAGTGTTTATTCTGGGTTTGTTGATTCTACAAATAGGGTTTTCGCTCGGGTAATTGAGCAAGGAGAGGTCATGGTGTTCCCCAGAGGTCTAGTGCACTTTCAAATGAATGTTGGCAAGAAGCCAGCTACAGTTTTTGGAAGCTTCAATAGCCAAAATCCAGGAATGCAAAAGATCCCAGCGGCTATTTTTGGGTCTGGTATAAATGATAAGCTCTTGGAAAAGGCATTTGGATTAAGTCCTAAGCAGATTGCGATGATGAGAAGAAGATTTGACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

218

Amino Acids

23.61

Weight (kDa)

9.67

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 67 - 208 7e-45 Cupin
Cupin_2 PF07883 100 - 169 1.2e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10080 AT3G10080
fragaria_vesca FvH4_6g18500
malus_domestica MD12G1024100.v1.1 MD12G1034800.v1.1 MD14G1021500.v1.1
prunus_persica Prupe.7G112300_v2.0.a1
pyrus_communis pycom12g02270
rosa_chinensis RchiOBHm_Chr3g0473041
rosa_laevigata RLG00000024035
rosa_multiflora Rmu_sc0006336.1_g000008
rosa_roxburghii Rroxscaffold_6G00408370
rosa_rugosa Rorug03G0130900
rosa_samantha Rh3BG209000 Rh3CG206100 Rh3DG206100
rosa_wichuraiana Rw3G016060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 202
AccIII TCCGGA 1 cut(s) 191
AciI CCGC 2 cut(s) 329, 554
AclWI GGATC 2 cut(s) 62, 541
AfaI GTAC 1 cut(s) 115
AgsI TTSAA 3 cut(s) 83, 476, 520
AjnI CCWGG 2 cut(s) 262, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 585, 617
AluBI AGCT 5 cut(s) 62, 299, 500, 516, 586
AluI AGCT 5 cut(s) 62, 299, 500, 516, 586
Alw21I GWGCWC 1 cut(s) 471
Alw44I GTGCAC 1 cut(s) 467
AlwI GGATC 2 cut(s) 62, 541
Ama87I CYCGRG 2 cut(s) 206, 420
Aor13HI TCCGGA 1 cut(s) 191
AoxI GGCC 2 cut(s) 165, 226
ApaLI GTGCAC 1 cut(s) 467
ApeKI GCWGC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 2 cut(s) 207, 208
AsuHPI GGTGA 3 cut(s) 163, 208, 256
AvaI CYCGRG 2 cut(s) 206, 420
BaeGI GKGCMC 1 cut(s) 471
BanI GGYRCC 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 1 cut(s) 245
BccI CCATC 3 cut(s) 146, 176, 245
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BciT130I CCWGG 2 cut(s) 264, 534
BcnI CCSGG 2 cut(s) 207, 208
BfaI CTAG 2 cut(s) 278, 464
BfmI CTRYAG 1 cut(s) 501
BisI GCNGC 2 cut(s) 234, 555
BlsI GCNGC 2 cut(s) 235, 556
Bme1390I CCNGG 4 cut(s) 207, 208, 264, 534
BmeT110I CYCGRG 2 cut(s) 206, 420
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 4 cut(s) 207, 208, 264, 534
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 178
BplI GAGNNNNNCTC 2 cut(s) 288, 320
Bpu10I CCTNAGC 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 2 cut(s) 207, 208
BsaJI CCNNGG 3 cut(s) 162, 206, 263
BsaWI WCCGGW 2 cut(s) 110, 191
Bse1I ACTGG 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 191
BseBI CCWGG 2 cut(s) 264, 534
BseDI CCNNGG 3 cut(s) 162, 206, 263
BseGI GGATG 3 cut(s) 36, 138, 288
BseNI ACTGG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 471
BseXI GCAGC 1 cut(s) 245
BseYI CCCAGC 1 cut(s) 550
BshFI GGCC 2 cut(s) 167, 228
BshNI GGYRCC 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 2 cut(s) 206, 420
BsiSI CCGG 3 cut(s) 111, 192, 207
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 2 cut(s) 167, 228
BsoBI CYCGRG 2 cut(s) 206, 420
Bsp1286I GDGCHC 1 cut(s) 471
Bsp13I TCCGGA 1 cut(s) 191
Bsp143I GATC 3 cut(s) 67, 301, 546
BspACI CCGC 2 cut(s) 329, 554
BspANI GGCC 2 cut(s) 167, 228
BspEI TCCGGA 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 204
BspPI GGATC 2 cut(s) 62, 541
BspT107I GGYRCC 1 cut(s) 202
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 162, 206, 263
BssMI GATC 3 cut(s) 67, 301, 546
Bst2UI CCWGG 2 cut(s) 264, 534
Bst4CI ACNGT 1 cut(s) 505
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 1 cut(s) 615
BstF5I GGATG 3 cut(s) 36, 138, 288
BstKTI GATC 3 cut(s) 70, 304, 549
BstMBI GATC 3 cut(s) 67, 301, 546
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNI CCWGG 2 cut(s) 264, 534
BstSCI CCNGG 4 cut(s) 205, 206, 262, 532
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 245
BstV2I GAAGAC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
BsuRI GGCC 2 cut(s) 167, 228
BtgZI GCGATG 1 cut(s) 641
BtsCI GGATG 3 cut(s) 36, 138, 288
Cac8I GCNNGC 1 cut(s) 498
Cfr13I GGNCC 1 cut(s) 226
Cfr9I CCCGGG 1 cut(s) 206
Csp6I GTAC 1 cut(s) 114
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 3 cut(s) 147, 223, 445
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 615
DpnI GATC 3 cut(s) 69, 303, 548
DpnII GATC 3 cut(s) 67, 301, 546
Eco88I CYCGRG 2 cut(s) 206, 420
EcoRII CCWGG 2 cut(s) 262, 532
FaeI CATG 3 cut(s) 150, 226, 448
FaiI YATR 8 cut(s) 23, 148, 224, 361, 363, 365, 446, 575
FatI CATG 3 cut(s) 146, 222, 444
Fnu4HI GCNGC 2 cut(s) 234, 555
FokI GGATG 3 cut(s) 23, 125, 295
Fsp4HI GCNGC 2 cut(s) 234, 555
FspBI CTAG 2 cut(s) 278, 464
GluI GCNGC 2 cut(s) 234, 555
GsaI CCCAGC 1 cut(s) 554
HaeIII GGCC 2 cut(s) 167, 228
HapII CCGG 3 cut(s) 111, 192, 207
Hin1II CATG 3 cut(s) 150, 226, 448
HindIII AAGCTT 1 cut(s) 514
HinfI GANTC 3 cut(s) 100, 318, 398
HpaII CCGG 3 cut(s) 111, 192, 207
HphI GGTGA 3 cut(s) 163, 208, 256
Hpy166II GTNNAC 2 cut(s) 247, 469
Hpy188I TCNGA 3 cut(s) 67, 105, 163
Hpy188III TCNNGA 3 cut(s) 71, 83, 192
Hpy8I GTNNAC 2 cut(s) 247, 469
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4V TGCA 3 cut(s) 150, 469, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 1 cut(s) 615
Hsp92II CATG 3 cut(s) 150, 226, 448
Kpn2I TCCGGA 1 cut(s) 191
Kzo9I GATC 3 cut(s) 67, 301, 546
Lsp1109I GCAGC 1 cut(s) 245
MaeI CTAG 2 cut(s) 278, 464
MaeIII GTNAC 1 cut(s) 3
MalI GATC 3 cut(s) 69, 303, 548
MboI GATC 3 cut(s) 67, 301, 546
MboII GAAGA 3 cut(s) 178, 648, 651
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 1 cut(s) 426
MnlI CCTC 6 cut(s) 50, 152, 157, 239, 433, 452
MroI TCCGGA 1 cut(s) 191
MseI TTAA 2 cut(s) 270, 608
MslI CAYNNNNRTG 2 cut(s) 221, 242
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 3 cut(s) 111, 192, 207
MspR9I CCNGG 4 cut(s) 207, 208, 264, 534
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 2 cut(s) 264, 534
MwoI GCNNNNNNNGC 1 cut(s) 522
NciI CCSGG 2 cut(s) 207, 208
NdeII GATC 3 cut(s) 67, 301, 546
NlaIII CATG 3 cut(s) 150, 226, 448
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 1 cut(s) 543
PfeI GAWTC 3 cut(s) 100, 318, 398
PflFI GACNNNGTC 1 cut(s) 182
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 2 cut(s) 235, 556
Psp6I CCWGG 2 cut(s) 262, 532
PspFI CCCAGC 1 cut(s) 550
PspGI CCWGG 2 cut(s) 262, 532
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 226
PsuI RGATCY 1 cut(s) 546
PsyI GACNNNGTC 1 cut(s) 182
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 2 cut(s) 221, 242
SaqAI TTAA 2 cut(s) 270, 608
SatI GCNGC 2 cut(s) 234, 555
Sau3AI GATC 3 cut(s) 67, 301, 546
Sau96I GGNCC 1 cut(s) 226
ScrFI CCNGG 4 cut(s) 207, 208, 264, 534
SduI GDGCHC 1 cut(s) 471
SetI ASST 8 cut(s) 64, 301, 312, 444, 463, 502, 518, 588
SfcI CTRYAG 1 cut(s) 501
SmaI CCCGGG 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 221, 242
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 1 cut(s) 426
SsiI CCGC 2 cut(s) 329, 554
SspMI CTAG 2 cut(s) 278, 464
StyD4I CCNGG 4 cut(s) 205, 206, 262, 532
TaaI ACNGT 1 cut(s) 505
TasI AATT 1 cut(s) 426
TauI GCSGC 1 cut(s) 557
TfiI GAWTC 3 cut(s) 100, 318, 398
Tru1I TTAA 2 cut(s) 270, 608
Tru9I TTAA 2 cut(s) 270, 608
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 25, 212, 494
TspMI CCCGGG 1 cut(s) 206
Tth111I GACNNNGTC 1 cut(s) 182
VneI GTGCAC 1 cut(s) 467
XmaI CCCGGG 1 cut(s) 206
XspI CTAG 2 cut(s) 278, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.