pycom12g02270

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014365
Physical Location & Seq
Reverse (-)
1978565 .. 1979239
675 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02270.1

Sequence Viewer

Length: 675 bp
ATGGCCAAGAAAATGTCTTCCGGTATCACAATTTTGCTTGTTATTTTTGCCCTCCTATGCGATGTTATGGCTTCCGATCCTGACCCGATTCAAGACTACTGCATACCGAACCCGAAATTTGGTGCCATAAGAACACCTCTTCACACCATCCACCTATGCAAGAACTCGTCCGAGGCCACCACCGATGACTTCATCTTCTCTGGGATGAAGGTGGCCGGAAACTTCAGCGACATGGGTATCGCAGCCATCTCAGTGAACCCAACTGTCTTCCCGGGGATCAACACGCTCGGGATGTCATTCGTACGAGCTGACCTCAAAGTTGGTGGAATCAATCCACCACATTTCCACCCTAGAGCCACAGAGATATCCCATGTAGTGAAGGGGAGTGTTTATTCAGGGTTTGTTGATTCAACCAATAGGGTTTTTGCTAGGGTTATTGAGCAAGGGGAGGTCATGGTGTTCCCCAGGGGTCTAGTGCACTTCCAAATGAATGTTGGCAAGAAGCCTGCTACAATTTTTGGTAGCTTCAATAGCCAAAACCCAGGAAGTCAAAGGATCCCCTCTGCCATTTTCGGGTCAGGGATAAACGACGAGCTCTTGGAGAAGGCATTTGGATTGAGTCCTAAGCAGATTGGAACGATGAGAAGAAGATTTGATCCAAAAAGTGTGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

225

Amino Acids

24.25

Weight (kDa)

9.24

Isoelectric Point (pI)

28.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 75 - 211 4.2e-43 Cupin
Cupin_2 PF07883 103 - 172 7.8e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10080 AT3G10080
fragaria_vesca FvH4_6g18500
malus_domestica MD12G1024100.v1.1 MD12G1034800.v1.1 MD14G1021500.v1.1
prunus_persica Prupe.7G112300_v2.0.a1
pyrus_communis pycom12g02270
rosa_chinensis RchiOBHm_Chr3g0473041
rosa_laevigata RLG00000024035
rosa_multiflora Rmu_sc0006336.1_g000008
rosa_roxburghii Rroxscaffold_6G00408370
rosa_rugosa Rorug03G0130900
rosa_samantha Rh3BG209000 Rh3CG206100 Rh3DG206100
rosa_wichuraiana Rw3G016060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AclWI GGATC 5 cut(s) 71, 284, 550, 563, 650
AcoI YGGCCR 2 cut(s) 3, 213
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 208
AfaI GTAC 1 cut(s) 303
AfiI CCNNNNNNNGG 2 cut(s) 119, 573
AgsI TTSAA 3 cut(s) 92, 411, 529
AjnI CCWGG 2 cut(s) 464, 541
AluBI AGCT 3 cut(s) 308, 525, 595
AluI AGCT 3 cut(s) 308, 525, 595
Alw21I GWGCWC 2 cut(s) 480, 597
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 5 cut(s) 71, 284, 550, 563, 650
Ama87I CYCGRG 2 cut(s) 271, 287
AoxI GGCC 3 cut(s) 3, 174, 213
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 1 cut(s) 116
AsuC2I CCSGG 2 cut(s) 272, 273
AvaI CYCGRG 2 cut(s) 271, 287
BaeGI GKGCMC 1 cut(s) 480
BaeI ACNNNNGTAYC 2 cut(s) 220, 253
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 555
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 597
BbsI GAAGAC 2 cut(s) 9, 259
Bbv12I GWGCWC 2 cut(s) 480, 597
BbvI GCAGC 1 cut(s) 254
BccI CCATC 2 cut(s) 155, 254
BciT130I CCWGG 2 cut(s) 466, 543
BcnI CCSGG 2 cut(s) 272, 273
BfaI CTAG 3 cut(s) 351, 429, 473
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 4 cut(s) 272, 273, 466, 543
BmeT110I CYCGRG 2 cut(s) 271, 287
BmiI GGNNCC 2 cut(s) 124, 557
BmrFI CCNGG 4 cut(s) 272, 273, 466, 543
BpiI GAAGAC 2 cut(s) 9, 259
BplI GAGNNNNNCTC 2 cut(s) 297, 329
Bpu10I CCTNAGC 1 cut(s) 624
BpuMI CCSGG 2 cut(s) 272, 273
BsaJI CCNNGG 6 cut(s) 171, 271, 272, 464, 465, 541
BsaWI WCCGGW 1 cut(s) 20
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 573
BseBI CCWGG 2 cut(s) 466, 543
BseDI CCNNGG 6 cut(s) 171, 271, 272, 464, 465, 541
BseGI GGATG 3 cut(s) 147, 210, 297
BseLI CCNNNNNNNGG 2 cut(s) 119, 573
BseMII CTCAG 1 cut(s) 264
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 1 cut(s) 254
BshFI GGCC 3 cut(s) 5, 176, 215
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 2 cut(s) 480, 597
BsiHKCI CYCGRG 2 cut(s) 271, 287
BsiSI CCGG 3 cut(s) 21, 216, 272
BsiWI CGTACG 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 119, 573
BsnI GGCC 3 cut(s) 5, 176, 215
BsoBI CYCGRG 2 cut(s) 271, 287
Bsp1286I GDGCHC 2 cut(s) 480, 597
Bsp143I GATC 4 cut(s) 76, 276, 555, 655
BspANI GGCC 3 cut(s) 5, 176, 215
BspCNI CTCAG 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 124, 557
BspPI GGATC 5 cut(s) 71, 284, 550, 563, 650
BspT107I GGYRCC 1 cut(s) 122
BssECI CCNNGG 6 cut(s) 171, 271, 272, 464, 465, 541
BssMI GATC 4 cut(s) 76, 276, 555, 655
Bst2UI CCWGG 2 cut(s) 466, 543
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 2 cut(s) 250, 624
BstF5I GGATG 3 cut(s) 147, 210, 297
BstKTI GATC 4 cut(s) 79, 279, 558, 658
BstMBI GATC 4 cut(s) 76, 276, 555, 655
BstMWI GCNNNNNNNGC 1 cut(s) 531
BstNI CCWGG 2 cut(s) 466, 543
BstSCI CCNGG 4 cut(s) 270, 271, 464, 541
BstSLI GKGCMC 1 cut(s) 480
BstV1I GCAGC 1 cut(s) 254
BstV2I GAAGAC 2 cut(s) 9, 259
BstX2I RGATCY 1 cut(s) 555
BstYI RGATCY 1 cut(s) 555
BsuRI GGCC 3 cut(s) 5, 176, 215
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 3 cut(s) 147, 210, 297
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 507
Cfr9I CCCGGG 1 cut(s) 271
Csp6I GTAC 1 cut(s) 302
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 3 cut(s) 232, 371, 454
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 2 cut(s) 250, 624
DpnI GATC 4 cut(s) 78, 278, 557, 657
DpnII GATC 4 cut(s) 76, 276, 555, 655
EaeI YGGCCR 2 cut(s) 3, 213
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 597
Eco32I GATATC 1 cut(s) 366
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 208
Eco88I CYCGRG 2 cut(s) 271, 287
EcoICRI GAGCTC 1 cut(s) 595
EcoRII CCWGG 2 cut(s) 464, 541
EcoRV GATATC 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 597
FaeI CATG 3 cut(s) 235, 374, 457
FaiI YATR 8 cut(s) 58, 68, 104, 128, 157, 233, 372, 455
FatI CATG 3 cut(s) 231, 370, 453
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 3 cut(s) 134, 217, 304
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 3 cut(s) 351, 429, 473
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 3 cut(s) 5, 176, 215
HapII CCGG 3 cut(s) 21, 216, 272
Hin1II CATG 3 cut(s) 235, 374, 457
HinfI GANTC 4 cut(s) 88, 327, 407, 619
HpaII CCGG 3 cut(s) 21, 216, 272
Hpy166II GTNNAC 2 cut(s) 256, 478
Hpy188I TCNGA 2 cut(s) 76, 172
Hpy188III TCNNGA 3 cut(s) 80, 92, 289
Hpy8I GTNNAC 2 cut(s) 256, 478
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 3 cut(s) 202, 373, 598
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 3 cut(s) 102, 159, 478
HpyF10VI GCNNNNNNNGC 1 cut(s) 531
HpyF3I CTNAG 2 cut(s) 250, 624
Hsp92II CATG 3 cut(s) 235, 374, 457
Kzo9I GATC 4 cut(s) 76, 276, 555, 655
Lsp1109I GCAGC 1 cut(s) 254
MaeI CTAG 3 cut(s) 351, 429, 473
MalI GATC 4 cut(s) 78, 278, 557, 657
MboI GATC 4 cut(s) 76, 276, 555, 655
MboII GAAGA 6 cut(s) 9, 131, 187, 259, 657, 660
MflI RGATCY 1 cut(s) 555
MhlI GDGCHC 2 cut(s) 480, 597
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 30, 116, 513
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 628
MnlI CCTC 6 cut(s) 62, 147, 166, 323, 442, 571
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 21, 216, 272
MspR9I CCNGG 4 cut(s) 272, 273, 466, 543
MvaI CCWGG 2 cut(s) 466, 543
MwoI GCNNNNNNNGC 1 cut(s) 531
NciI CCSGG 2 cut(s) 272, 273
NdeII GATC 4 cut(s) 76, 276, 555, 655
NlaIII CATG 3 cut(s) 235, 374, 457
NlaIV GGNNCC 2 cut(s) 124, 557
PasI CCCWGGG 1 cut(s) 465
PfeI GAWTC 3 cut(s) 88, 327, 407
Pfl23II CGTACG 1 cut(s) 301
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 627
PpsI GAGTC 1 cut(s) 627
Psp124BI GAGCTC 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 464, 541
PspGI CCWGG 2 cut(s) 464, 541
PspLI CGTACG 1 cut(s) 301
PspN4I GGNNCC 2 cut(s) 124, 557
PsuI RGATCY 1 cut(s) 555
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 251
SacI GAGCTC 1 cut(s) 597
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 4 cut(s) 76, 276, 555, 655
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 4 cut(s) 272, 273, 466, 543
SduI GDGCHC 2 cut(s) 480, 597
SetI ASST 8 cut(s) 139, 156, 213, 310, 315, 453, 527, 597
SmaI CCCGGG 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 251
Sse9I AATT 3 cut(s) 30, 116, 513
SspMI CTAG 3 cut(s) 351, 429, 473
SstI GAGCTC 1 cut(s) 597
StyD4I CCNGG 4 cut(s) 270, 271, 464, 541
TaaI ACNGT 1 cut(s) 265
TasI AATT 3 cut(s) 30, 116, 513
TfiI GAWTC 3 cut(s) 88, 327, 407
TscAI CASTG 1 cut(s) 258
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 3 cut(s) 181, 221, 503
TspMI CCCGGG 1 cut(s) 271
TspRI CASTG 1 cut(s) 258
VneI GTGCAC 1 cut(s) 476
XapI RAATTY 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
XmaI CCCGGG 1 cut(s) 271
XspI CTAG 3 cut(s) 351, 429, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.