MD12G1024100.v1.1

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014365
Physical Location & Seq
Reverse (-)
2451931 .. 2452593
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1024100.v1.1.491

Sequence Viewer

Length: 663 bp
ATGTCTTCCAATATCACAATTTTGCTTGTTATTTTTGCCCTCCTATGTGATGTTATGGCTTCCGATCCTGACCCGATTCAAGACTACTGCATACCGAACCCGAAATTTGGTGCCATAAGAACACCTCTTCACACCATCCTCCCATGCAAGAACTCGTCCGAGGCCACCACCGATGACTTCATCTTCTCTGGGATGAAGGTGGCCGGAAACTTCAGCGACATGGGTATCGCAGCCATCGCAGTGAACCCAACAGTCTTCCCGGGGATCAACACGCTCGGGATGTCATTCGTACGAGCTGACCTCATAGTTGGTGGAATCAATCCACCACATTTCCACCCTAGAGCCACAGAGATATCCCACGTAGTGAAGGGGAGTGTTTATTCAGGGTTCGTTGATTCAACCAATAGGGTTTTTGCTAGGGTTATTGAGCAAGGGGAGGTCATGGTGTTCCCTAGGGGTCTAGTGCACTTCCAAATGAATGTTGGCAAGAAGCCTGCTACAATTTTTGGTAGCTTCAACAGCCAAAACCCAGGAAGTCAAAGGATCCCCTCTGCCATTTTCGGGTCGGGGATAAACGATGAGCTCTTGGAGAAGGCATTTGGATTGAGTCCTAAGCAGATTGGAACGATGAGAAGAAGATTTGATCCAAAAAGTATGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

221

Amino Acids

23.81

Weight (kDa)

8.49

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 71 - 207 2.4e-42 Cupin
Cupin_2 PF07883 101 - 168 2.6e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10080 AT3G10080
fragaria_vesca FvH4_6g18500
malus_domestica MD12G1024100.v1.1 MD12G1034800.v1.1 MD14G1021500.v1.1
prunus_persica Prupe.7G112300_v2.0.a1
pyrus_communis pycom12g02270
rosa_chinensis RchiOBHm_Chr3g0473041
rosa_laevigata RLG00000024035
rosa_multiflora Rmu_sc0006336.1_g000008
rosa_roxburghii Rroxscaffold_6G00408370
rosa_rugosa Rorug03G0130900
rosa_samantha Rh3BG209000 Rh3CG206100 Rh3DG206100
rosa_wichuraiana Rw3G016060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AclWI GGATC 5 cut(s) 59, 272, 538, 551, 638
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 1 cut(s) 104
AcuI CTGAAG 1 cut(s) 196
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 291
AfiI CCNNNNNNNGG 2 cut(s) 107, 561
AgsI TTSAA 3 cut(s) 80, 399, 517
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 3 cut(s) 296, 513, 583
AluI AGCT 3 cut(s) 296, 513, 583
Alw21I GWGCWC 2 cut(s) 468, 585
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 5 cut(s) 59, 272, 538, 551, 638
Ama87I CYCGRG 2 cut(s) 259, 275
AoxI GGCC 2 cut(s) 162, 201
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 104
AspA2I CCTAGG 1 cut(s) 452
AsuC2I CCSGG 2 cut(s) 260, 261
AvaI CYCGRG 2 cut(s) 259, 275
AvrII CCTAGG 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 468
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BamHI GGATCC 1 cut(s) 543
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 2 cut(s) 468, 585
BbvI GCAGC 1 cut(s) 242
BccI CCATC 2 cut(s) 143, 242
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 2 cut(s) 260, 261
BfaI CTAG 4 cut(s) 339, 417, 453, 461
BisI GCNGC 1 cut(s) 231
BlnI CCTAGG 1 cut(s) 452
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 3 cut(s) 260, 261, 531
BmeT110I CYCGRG 2 cut(s) 259, 275
BmiI GGNNCC 2 cut(s) 112, 545
BmrFI CCNGG 3 cut(s) 260, 261, 531
BpiI GAAGAC 1 cut(s) 247
BplI GAGNNNNNCTC 2 cut(s) 285, 317
Bpu10I CCTNAGC 1 cut(s) 612
BpuMI CCSGG 2 cut(s) 260, 261
BsaAI YACGTR 1 cut(s) 361
BsaJI CCNNGG 5 cut(s) 159, 259, 260, 452, 529
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 561
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 5 cut(s) 159, 259, 260, 452, 529
BseGI GGATG 3 cut(s) 135, 198, 285
BseLI CCNNNNNNNGG 2 cut(s) 107, 561
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 2 cut(s) 164, 203
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 2 cut(s) 468, 585
BsiHKCI CYCGRG 2 cut(s) 259, 275
BsiSI CCGG 2 cut(s) 204, 260
BsiWI CGTACG 1 cut(s) 289
BslI CCNNNNNNNGG 2 cut(s) 107, 561
BsnI GGCC 2 cut(s) 164, 203
BsoBI CYCGRG 2 cut(s) 259, 275
Bsp1286I GDGCHC 2 cut(s) 468, 585
Bsp143I GATC 4 cut(s) 64, 264, 543, 643
BspANI GGCC 2 cut(s) 164, 203
BspLI GGNNCC 2 cut(s) 112, 545
BspPI GGATC 5 cut(s) 59, 272, 538, 551, 638
BspT107I GGYRCC 1 cut(s) 110
BssECI CCNNGG 5 cut(s) 159, 259, 260, 452, 529
BssMI GATC 4 cut(s) 64, 264, 543, 643
BssT1I CCWWGG 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 132
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 1 cut(s) 612
BstF5I GGATG 3 cut(s) 135, 198, 285
BstKTI GATC 4 cut(s) 67, 267, 546, 646
BstMBI GATC 4 cut(s) 64, 264, 543, 643
BstMWI GCNNNNNNNGC 2 cut(s) 236, 519
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 3 cut(s) 258, 259, 529
BstSLI GKGCMC 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 242
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 1 cut(s) 543
BstYI RGATCY 1 cut(s) 543
BsuRI GGCC 2 cut(s) 164, 203
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 3 cut(s) 135, 198, 285
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 495
Cfr9I CCCGGG 1 cut(s) 259
Csp6I GTAC 1 cut(s) 290
CviAII CATG 3 cut(s) 144, 220, 442
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 1 cut(s) 612
DpnI GATC 4 cut(s) 66, 266, 545, 645
DpnII GATC 4 cut(s) 64, 264, 543, 643
DraIII CACNNNGTG 1 cut(s) 364
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 585
Eco32I GATATC 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 196
Eco88I CYCGRG 2 cut(s) 259, 275
EcoICRI GAGCTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 452
FaeI CATG 3 cut(s) 147, 223, 445
FaiI YATR 9 cut(s) 46, 56, 92, 116, 145, 221, 305, 443, 656
FatI CATG 3 cut(s) 143, 219, 441
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 3 cut(s) 122, 205, 292
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 4 cut(s) 339, 417, 453, 461
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 2 cut(s) 164, 203
HapII CCGG 2 cut(s) 204, 260
Hin1II CATG 3 cut(s) 147, 223, 445
HinfI GANTC 4 cut(s) 76, 315, 395, 607
HpaII CCGG 2 cut(s) 204, 260
Hpy166II GTNNAC 2 cut(s) 244, 466
Hpy188I TCNGA 2 cut(s) 64, 160
Hpy188III TCNNGA 3 cut(s) 68, 80, 277
Hpy8I GTNNAC 2 cut(s) 244, 466
HpyAV CCTTC 3 cut(s) 190, 361, 586
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 3 cut(s) 90, 147, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 519
HpyF3I CTNAG 1 cut(s) 612
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 3 cut(s) 147, 223, 445
Kzo9I GATC 4 cut(s) 64, 264, 543, 643
LpnPI CCDG 8 cut(s) 81, 174, 217, 273, 369, 507, 516, 543
Lsp1109I GCAGC 1 cut(s) 242
MaeI CTAG 4 cut(s) 339, 417, 453, 461
MaeII ACGT 1 cut(s) 360
MalI GATC 4 cut(s) 66, 266, 545, 645
MboI GATC 4 cut(s) 64, 264, 543, 643
MboII GAAGA 5 cut(s) 119, 175, 247, 645, 648
MflI RGATCY 1 cut(s) 543
MhlI GDGCHC 2 cut(s) 468, 585
MluCI AATT 3 cut(s) 18, 104, 501
MlyI GAGTC 1 cut(s) 616
MnlI CCTC 7 cut(s) 50, 135, 149, 154, 311, 430, 559
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 2 cut(s) 204, 260
MspR9I CCNGG 3 cut(s) 260, 261, 531
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 236, 519
NciI CCSGG 2 cut(s) 260, 261
NdeII GATC 4 cut(s) 64, 264, 543, 643
NlaIII CATG 3 cut(s) 147, 223, 445
NlaIV GGNNCC 2 cut(s) 112, 545
PfeI GAWTC 3 cut(s) 76, 315, 395
Pfl23II CGTACG 1 cut(s) 289
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
Ppu21I YACGTR 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspLI CGTACG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 112, 545
PsuI RGATCY 1 cut(s) 543
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 585
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 4 cut(s) 64, 264, 543, 643
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 3 cut(s) 260, 261, 531
SduI GDGCHC 2 cut(s) 468, 585
SetI ASST 8 cut(s) 127, 201, 298, 303, 363, 441, 515, 585
SmaI CCCGGG 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 239
Sse9I AATT 3 cut(s) 18, 104, 501
SspMI CTAG 4 cut(s) 339, 417, 453, 461
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 258, 259, 529
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 1 cut(s) 363
TasI AATT 3 cut(s) 18, 104, 501
TfiI GAWTC 3 cut(s) 76, 315, 395
TscAI CASTG 1 cut(s) 246
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 169, 209, 491
TspMI CCCGGG 1 cut(s) 259
TspRI CASTG 1 cut(s) 246
VneI GTGCAC 1 cut(s) 464
XapI RAATTY 1 cut(s) 104
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XmaI CCCGGG 1 cut(s) 259
XmaJI CCTAGG 1 cut(s) 452
XspI CTAG 4 cut(s) 339, 417, 453, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.