MD14G1021500.v1.1

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014365
Physical Location & Seq
Reverse (-)
2010306 .. 2010977
672 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1021500.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGCCCAGAAAATGTCTTCTAGTATCACAATTTTGCTTGTTATTTTTGCCCTCCAATGCGCCATAACATTGGCTTCCGATCCTGATCCGGTTCAAGACTTCTGCATACCAAACCCGAAATTCGGTGCCCTGAGAACACCTCTTCACAACATCCTCCCATGCAAGAACGCGTCCGAGGCCACCACTGATGACTTCATCTTCTCTGGAATGAAGGTGGCCGGGAACTTCACCGACATGGGCATCGCAGCCATCTCGGTGAACCCAACGGTCTTCCCGGGGATCAACACGCTGGGGATGTCATTCATACGATCTGACCTCAAAGTTGGTGGAATCAATCCACCACACTTCCACCCTAGAGCCACAGAGATATCCCACGTAGTGGAAGGGAGTGTTTATTCAGGGTTTGTTGATTCAGCCAATAGGGTTTTTGCTAGGGTTATTGAGCAAGGGGAGGTCATGGTGTTCCCTAGGGGTCTAGTGCACTTCCAGATGAATGTTGGCAAGAATCCTGCTACAATATTTGGTAGCTTCAATAGCCAAAACCCAGGAAATCAAAAGATTCCCTCTGCCATTTTCGGGTCGCGGATAAACAGTGAGCTCTTGGAGAAGGCATTTGGATTGAGTCCTAAGCAGATTAGAACGATGAGAAGAAAATTTGATCCAAAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

224

Amino Acids

24.3

Weight (kDa)

9.47

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 76 - 212 2.7e-43 Cupin
Cupin_2 PF07883 105 - 173 1.7e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014365)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10080 AT3G10080
fragaria_vesca FvH4_6g18500
malus_domestica MD12G1024100.v1.1 MD12G1034800.v1.1 MD14G1021500.v1.1
prunus_persica Prupe.7G112300_v2.0.a1
pyrus_communis pycom12g02270
rosa_chinensis RchiOBHm_Chr3g0473041
rosa_laevigata RLG00000024035
rosa_multiflora Rmu_sc0006336.1_g000008
rosa_roxburghii Rroxscaffold_6G00408370
rosa_rugosa Rorug03G0130900
rosa_samantha Rh3BG209000 Rh3CG206100 Rh3DG206100
rosa_wichuraiana Rw3G016060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 379
AccII CGCG 2 cut(s) 170, 583
AciI CCGC 1 cut(s) 583
AclWI GGATC 4 cut(s) 74, 80, 287, 653
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 2 cut(s) 119, 653
AdeI CACNNNGTG 1 cut(s) 379
AfiI CCNNNNNNNGG 3 cut(s) 122, 379, 576
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 2 cut(s) 95, 532
AjnI CCWGG 1 cut(s) 544
AluBI AGCT 2 cut(s) 528, 598
AluI AGCT 2 cut(s) 528, 598
Alw21I GWGCWC 2 cut(s) 483, 600
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 4 cut(s) 74, 80, 287, 653
Ama87I CYCGRG 1 cut(s) 274
AoxI GGCC 3 cut(s) 3, 177, 216
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 2 cut(s) 119, 653
AspA2I CCTAGG 1 cut(s) 467
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 3 cut(s) 220, 275, 276
AsuHPI GGTGA 2 cut(s) 220, 268
AvaI CYCGRG 1 cut(s) 274
AvrII CCTAGG 1 cut(s) 467
BaeGI GKGCMC 2 cut(s) 130, 483
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 600
BbsI GAAGAC 2 cut(s) 9, 262
Bbv12I GWGCWC 2 cut(s) 483, 600
BbvI GCAGC 1 cut(s) 257
BccI CCATC 1 cut(s) 257
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 1 cut(s) 546
BcnI CCSGG 3 cut(s) 220, 275, 276
BfaI CTAG 5 cut(s) 21, 354, 432, 468, 476
BisI GCNGC 1 cut(s) 246
BlnI CCTAGG 1 cut(s) 467
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 4 cut(s) 220, 275, 276, 546
BmeT110I CYCGRG 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 4 cut(s) 220, 275, 276, 546
BmsI GCATC 1 cut(s) 249
BpiI GAAGAC 2 cut(s) 9, 262
BplI GAGNNNNNCTC 2 cut(s) 124, 156
Bpu10I CCTNAGC 1 cut(s) 627
BpuMI CCSGG 3 cut(s) 220, 275, 276
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 1 cut(s) 84
BsaJI CCNNGG 5 cut(s) 174, 274, 275, 467, 544
BsaWI WCCGGW 1 cut(s) 88
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 379, 576
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 5 cut(s) 174, 274, 275, 467, 544
BseGI GGATG 2 cut(s) 150, 300
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 3 cut(s) 122, 379, 576
BseMII CTCAG 1 cut(s) 122
BseSI GKGCMC 2 cut(s) 130, 483
BseXI GCAGC 1 cut(s) 257
BseYI CCCAGC 1 cut(s) 289
Bsh1236I CGCG 2 cut(s) 170, 583
BshFI GGCC 3 cut(s) 5, 179, 218
BshNI GGYRCC 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 483, 600
BsiHKCI CYCGRG 1 cut(s) 274
BsiSI CCGG 3 cut(s) 89, 219, 275
BslI CCNNNNNNNGG 3 cut(s) 122, 379, 576
BsnI GGCC 3 cut(s) 5, 179, 218
BsoBI CYCGRG 1 cut(s) 274
Bsp1286I GDGCHC 3 cut(s) 130, 483, 600
Bsp143I GATC 5 cut(s) 79, 85, 279, 308, 658
BspACI CCGC 1 cut(s) 583
BspANI GGCC 3 cut(s) 5, 179, 218
BspCNI CTCAG 1 cut(s) 123
BspFNI CGCG 2 cut(s) 170, 583
BspLI GGNNCC 1 cut(s) 127
BspPI GGATC 4 cut(s) 74, 80, 287, 653
BspT107I GGYRCC 1 cut(s) 125
BssECI CCNNGG 5 cut(s) 174, 274, 275, 467, 544
BssMI GATC 5 cut(s) 79, 85, 279, 308, 658
BssT1I CCWWGG 1 cut(s) 467
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 2 cut(s) 268, 593
Bst6I CTCTTC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 376
BstDEI CTNAG 2 cut(s) 131, 627
BstF5I GGATG 2 cut(s) 150, 300
BstFNI CGCG 2 cut(s) 170, 583
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 5 cut(s) 82, 88, 282, 311, 661
BstMBI GATC 5 cut(s) 79, 85, 279, 308, 658
BstMWI GCNNNNNNNGC 2 cut(s) 176, 534
BstNI CCWGG 1 cut(s) 546
BstSCI CCNGG 4 cut(s) 218, 273, 274, 544
BstSLI GKGCMC 2 cut(s) 130, 483
BstUI CGCG 2 cut(s) 170, 583
BstV1I GCAGC 1 cut(s) 257
BstV2I GAAGAC 2 cut(s) 9, 262
BstXI CCANNNNNNTGG 1 cut(s) 70
BsuRI GGCC 3 cut(s) 5, 179, 218
BtgZI GCGATG 1 cut(s) 226
BtsCI GGATG 2 cut(s) 150, 300
BtsIMutI CAGTG 2 cut(s) 183, 598
CfoI GCGC 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 4
Cfr9I CCCGGG 1 cut(s) 274
CseI GACGC 1 cut(s) 159
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 3 cut(s) 159, 235, 457
DdeI CTNAG 2 cut(s) 131, 627
DpnI GATC 5 cut(s) 81, 87, 281, 310, 660
DpnII GATC 5 cut(s) 79, 85, 279, 308, 658
DraIII CACNNNGTG 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 467
Eco24I GRGCYC 1 cut(s) 600
Eco32I GATATC 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 274
EcoICRI GAGCTC 1 cut(s) 598
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 467
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 467
FaeI CATG 3 cut(s) 162, 238, 460
FaiI YATR 6 cut(s) 65, 107, 160, 236, 305, 458
FatI CATG 3 cut(s) 158, 234, 456
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 2 cut(s) 137, 307
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 5 cut(s) 21, 354, 432, 468, 476
GlaI GCGC 1 cut(s) 61
GluI GCNGC 1 cut(s) 246
GsaI CCCAGC 1 cut(s) 293
HaeIII GGCC 3 cut(s) 5, 179, 218
HapII CCGG 3 cut(s) 89, 219, 275
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 62
Hin1II CATG 3 cut(s) 162, 238, 460
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HinfI GANTC 5 cut(s) 330, 410, 505, 559, 622
HpaII CCGG 3 cut(s) 89, 219, 275
HphI GGTGA 2 cut(s) 220, 268
Hpy166II GTNNAC 2 cut(s) 259, 481
Hpy188I TCNGA 3 cut(s) 79, 175, 313
Hpy188III TCNNGA 4 cut(s) 83, 95, 204, 487
Hpy8I GTNNAC 2 cut(s) 259, 481
HpyAV CCTTC 3 cut(s) 205, 377, 601
HpyCH4III ACNGT 2 cut(s) 268, 593
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 3 cut(s) 105, 162, 481
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 534
HpyF3I CTNAG 2 cut(s) 131, 627
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 3 cut(s) 162, 238, 460
HspAI GCGC 1 cut(s) 60
Kzo9I GATC 5 cut(s) 79, 85, 279, 308, 658
Lsp1109I GCAGC 1 cut(s) 257
LweI GCATC 1 cut(s) 249
MaeI CTAG 5 cut(s) 21, 354, 432, 468, 476
MaeII ACGT 1 cut(s) 375
MalI GATC 5 cut(s) 81, 87, 281, 310, 660
MboI GATC 5 cut(s) 79, 85, 279, 308, 658
MboII GAAGA 5 cut(s) 9, 134, 190, 262, 660
MhlI GDGCHC 3 cut(s) 130, 483, 600
MluCI AATT 3 cut(s) 30, 119, 653
MluI ACGCGT 1 cut(s) 168
MlyI GAGTC 1 cut(s) 631
MnlI CCTC 7 cut(s) 62, 150, 164, 169, 326, 445, 574
MslI CAYNNNNRTG 2 cut(s) 233, 254
MspI CCGG 3 cut(s) 89, 219, 275
MspR9I CCNGG 4 cut(s) 220, 275, 276, 546
MvaI CCWGG 1 cut(s) 546
MvnI CGCG 2 cut(s) 170, 583
MwoI GCNNNNNNNGC 2 cut(s) 176, 534
NciI CCSGG 3 cut(s) 220, 275, 276
NdeII GATC 5 cut(s) 79, 85, 279, 308, 658
NlaIII CATG 3 cut(s) 162, 238, 460
NlaIV GGNNCC 1 cut(s) 127
PfeI GAWTC 4 cut(s) 330, 410, 505, 559
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 1 cut(s) 247
PleI GAGTC 1 cut(s) 630
PpsI GAGTC 1 cut(s) 630
Ppu21I YACGTR 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 289
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 233, 254
SacI GAGCTC 1 cut(s) 600
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 5 cut(s) 79, 85, 279, 308, 658
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 4 cut(s) 220, 275, 276, 546
SduI GDGCHC 3 cut(s) 130, 483, 600
SetI ASST 8 cut(s) 142, 216, 318, 378, 456, 530, 600, 671
SfaNI GCATC 1 cut(s) 249
SmaI CCCGGG 1 cut(s) 276
SmiMI CAYNNNNRTG 2 cut(s) 233, 254
Sse9I AATT 3 cut(s) 30, 119, 653
SsiI CCGC 1 cut(s) 583
SspI AATATT 1 cut(s) 519
SspMI CTAG 5 cut(s) 21, 354, 432, 468, 476
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 4 cut(s) 218, 273, 274, 544
StyI CCWWGG 1 cut(s) 467
TaaI ACNGT 2 cut(s) 268, 593
TaiI ACGT 1 cut(s) 378
TasI AATT 3 cut(s) 30, 119, 653
TfiI GAWTC 4 cut(s) 330, 410, 505, 559
TscAI CASTG 2 cut(s) 190, 598
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 4 cut(s) 184, 224, 292, 506
TspMI CCCGGG 1 cut(s) 274
TspRI CASTG 2 cut(s) 190, 598
Van91I CCANNNNNTGG 1 cut(s) 379
VneI GTGCAC 1 cut(s) 479
XapI RAATTY 2 cut(s) 119, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmaI CCCGGG 1 cut(s) 274
XmaJI CCTAGG 1 cut(s) 467
XspI CTAG 5 cut(s) 21, 354, 432, 468, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.