FvH4_6g21311

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0020123
Physical Location & Seq
Forward (+)
14860103 .. 14860947
845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21311.t1

Sequence Viewer

Length: 612 bp
ATGTGGGAATCTGAGTTGTTAAAGTGTGGTGAGAAGCATGAAAATTTGGCATTTTCCAAGACAGTTGTGCTCAAATGGTGGAAACCAACACCTGGGGTGGGTAAACTTAACACTGATGGGTCAATGAACCCCATAAATGGCCGCTTTGGGGGTGACGGGGTGATTAGGAAATCTGATGGAAATTGGTTATATGGCTTCTTCGCCAACTTTGGGTTTGGAACCGTTCTTAAAGTAGAGGCTGAAGCTCTATATGTTGGATTGACAATCGCCAGTGAACTTCATCTTACGAGTTTGGAGATCGAGACAGATTCTTCTATTCTTGTCCTATTGGTGAAAGATTCAGCCAAACTTGTTGTTTCTGAGCAGCTGCGTCAAACAATTATTAACTGCCAAAATCTTCTAAAGTCTTTTCAGCATTATATTTTTGAGCATGTTTACAGAGAGCAAAACATTATGGTTGATAAGCTTGCTGCTAAAGATTGTGAGCAAGAAGGCCTACATAAGTTTGGTTGTCCTCCGGACTTCTTTACTGATGCGTTGGAGAATGATCTCTGTGGAAAAACCACTCCTAGACAGATGGGAAAGGCGAAAAATGGTAGGAAGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.77

Weight (kDa)

6.95

Isoelectric Point (pI)

20.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 37 - 158 2.3e-21 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020123)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g21311
prunus_persica Prupe.3G010000_v2.0.a1 Prupe.4G174500_v2.0.a1 Prupe.6G123500_v2.0.a1
pyrus_communis pycom10g09650
rosa_rugosa Rorug02G0245200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 1 cut(s) 142
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 261
AfiI CCNNNNNNNGG 5 cut(s) 92, 98, 137, 148, 210
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 3 cut(s) 245, 367, 466
AluI AGCT 3 cut(s) 245, 367, 466
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 2 cut(s) 139, 493
ApeKI GCWGC 3 cut(s) 364, 367, 470
ApoI RAATTY 1 cut(s) 43
AsuHPI GGTGA 4 cut(s) 41, 164, 172, 343
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 3 cut(s) 354, 376, 457
BccI CCATC 3 cut(s) 110, 170, 571
BciT130I CCWGG 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 1 cut(s) 570
BisI GCNGC 4 cut(s) 142, 365, 368, 471
BlsI GCNGC 4 cut(s) 143, 366, 369, 472
Bme1390I CCNGG 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 523
BsaJI CCNNGG 1 cut(s) 92
BsaWI WCCGGW 1 cut(s) 517
Bsc4I CCNNNNNNNGG 5 cut(s) 92, 98, 137, 148, 210
Bse1I ACTGG 1 cut(s) 270
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 92
BseLI CCNNNNNNNGG 5 cut(s) 92, 98, 137, 148, 210
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 270
BseXI GCAGC 3 cut(s) 354, 376, 457
BshFI GGCC 2 cut(s) 141, 495
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 518
BslI CCNNNNNNNGG 5 cut(s) 92, 98, 137, 148, 210
BsmAI GTCTC 1 cut(s) 296
BsnI GGCC 2 cut(s) 141, 495
Bsp1286I GDGCHC 1 cut(s) 72
Bsp13I TCCGGA 1 cut(s) 517
Bsp143I GATC 2 cut(s) 297, 547
BspACI CCGC 1 cut(s) 142
BspANI GGCC 2 cut(s) 141, 495
BspCNI CTCAG 2 cut(s) 4, 352
BspEI TCCGGA 1 cut(s) 517
BspLI GGNNCC 1 cut(s) 220
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 2 cut(s) 297, 547
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 64, 223
BstC8I GCNNGC 1 cut(s) 468
BstDEI CTNAG 2 cut(s) 12, 360
BstKTI GATC 2 cut(s) 300, 550
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 2 cut(s) 297, 547
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 434
BstSCI CCNGG 1 cut(s) 91
BstV1I GCAGC 3 cut(s) 354, 376, 457
BsuRI GGCC 2 cut(s) 141, 495
BtsIMutI CAGTG 2 cut(s) 111, 277
Cac8I GCNNGC 1 cut(s) 468
CseI GACGC 1 cut(s) 359
CviAII CATG 2 cut(s) 38, 431
CviJI RGCY 8 cut(s) 141, 195, 239, 245, 344, 367, 466, 495
CviKI_1 RGCY 8 cut(s) 141, 195, 239, 245, 344, 367, 466, 495
DdeI CTNAG 2 cut(s) 12, 360
DpnI GATC 2 cut(s) 299, 549
DpnII GATC 2 cut(s) 297, 547
EaeI YGGCCR 1 cut(s) 139
Eco147I AGGCCT 1 cut(s) 495
Eco57I CTGAAG 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 91
FaeI CATG 2 cut(s) 41, 434
FatI CATG 2 cut(s) 37, 430
Fnu4HI GCNGC 4 cut(s) 142, 365, 368, 471
Fsp4HI GCNGC 4 cut(s) 142, 365, 368, 471
FspBI CTAG 1 cut(s) 570
GluI GCNGC 4 cut(s) 142, 365, 368, 471
HaeIII GGCC 2 cut(s) 141, 495
HapII CCGG 1 cut(s) 518
HgaI GACGC 1 cut(s) 359
Hin1II CATG 2 cut(s) 41, 434
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 3 cut(s) 8, 308, 338
HpaII CCGG 1 cut(s) 518
HphI GGTGA 4 cut(s) 41, 164, 172, 343
Hpy166II GTNNAC 3 cut(s) 104, 275, 436
Hpy188I TCNGA 3 cut(s) 13, 175, 361
Hpy188III TCNNGA 2 cut(s) 301, 518
Hpy8I GTNNAC 3 cut(s) 104, 275, 436
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 2 cut(s) 64, 223
HpyF3I CTNAG 2 cut(s) 12, 360
Hsp92II CATG 2 cut(s) 41, 434
Kpn2I TCCGGA 1 cut(s) 517
Kzo9I GATC 2 cut(s) 297, 547
LpnPI CCDG 4 cut(s) 78, 105, 283, 531
Lsp1109I GCAGC 3 cut(s) 354, 376, 457
LweI GCATC 1 cut(s) 523
MaeI CTAG 1 cut(s) 570
MaeIII GTNAC 1 cut(s) 152
MalI GATC 2 cut(s) 299, 549
MboI GATC 2 cut(s) 297, 547
MboII GAAGA 3 cut(s) 190, 303, 389
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 3 cut(s) 43, 181, 378
MmeI TCCRAC 2 cut(s) 235, 519
MnlI CCTC 2 cut(s) 229, 525
MroI TCCGGA 1 cut(s) 517
MseI TTAA 4 cut(s) 20, 108, 228, 384
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
NdeII GATC 2 cut(s) 297, 547
NlaIII CATG 2 cut(s) 41, 434
NlaIV GGNNCC 1 cut(s) 220
NmuCI GTSAC 1 cut(s) 152
NspI RCATGY 1 cut(s) 434
PceI AGGCCT 1 cut(s) 495
PfeI GAWTC 3 cut(s) 8, 308, 338
PflMI CCANNNNNTGG 1 cut(s) 92
PkrI GCNGC 4 cut(s) 143, 366, 369, 472
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 367
SaqAI TTAA 4 cut(s) 20, 108, 228, 384
SatI GCNGC 4 cut(s) 142, 365, 368, 471
Sau3AI GATC 2 cut(s) 297, 547
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 1 cut(s) 72
SetI ASST 4 cut(s) 94, 247, 369, 468
SfaNI GCATC 1 cut(s) 523
Sse9I AATT 3 cut(s) 43, 181, 378
SseBI AGGCCT 1 cut(s) 495
SsiI CCGC 1 cut(s) 142
SspMI CTAG 1 cut(s) 570
StuI AGGCCT 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 2 cut(s) 64, 223
TaqI TCGA 1 cut(s) 300
TasI AATT 3 cut(s) 43, 181, 378
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 3 cut(s) 8, 308, 338
Tru1I TTAA 4 cut(s) 20, 108, 228, 384
Tru9I TTAA 4 cut(s) 20, 108, 228, 384
TscAI CASTG 2 cut(s) 118, 277
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 3 cut(s) 364, 367, 470
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 3 cut(s) 54, 140, 269
TspRI CASTG 2 cut(s) 118, 277
Van91I CCANNNNNTGG 1 cut(s) 92
XapI RAATTY 1 cut(s) 43
XceI RCATGY 1 cut(s) 434
XspI CTAG 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.