Rorug02G0245200

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0020123
Physical Location & Seq
Forward (+)
25488696 .. 25489539
844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0245200.1

Sequence Viewer

Length: 768 bp
ATGCAACACTGTAAGCCTCCACAGAGGCGGTTTCCGTTGGAGAAAGGTTTGGCTCCGCCGTGGTGGCCTGACGGAAATGAGATATGGTGGGGTGAACAATGGGTTTCTCAAGAGCATGGGCCTCCTCCATATAGGAAACCTCATGATCTCAAAAAGGCTTGGAAAGTAAGTGCTTTGGCTGCAGTGATTAAGCATATGGTTCCTAATTTGGACAGGTGGTTAACCAAGAAGAAGCAATTCTCCAACTTATTACTGATCAGAAACCAATCAGCTGCCCCGAAATCGCGGGCACCACAGCCTTACATTCTAACGAAAAGAGGAAGTGCATGTTTGATGACGATTTCTCTGAATTGGATCGATGCTCCACATGTTCTTCCAAAAACTTCAAACAGTGAATTAGGTTTAGCCTTTGTGGATAAGAAATCAAGGGCAGGCCATGATCAAAATAATGTTTCTGATTCAATGCGGGAGGTTGACACAAGTTCTTCGTATGATCATTCCTCAAATGATTCAGGATTCGAAAGTGTGGTGGAATGGATTAATATGGAGCTACAAAAGGCCAGCATAAACGAAGTCGGTGGAGTGGTCCTGTCTGGTTTTCCAAATACTTACTGGGAAGGTGGAATGGAAGAATTAGCTTTGGATGCAGCATTTGAGATTCAAAGGCAAAACATGGATTTGAATTTATGTCCAGAAGAAGGGATCTCACAGGTGAATCAAGACTCAACTTCAATTTGGGACATGGGATATGATTATGATAATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

255

Amino Acids

28.92

Weight (kDa)

5.66

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIN3_DNA-bd PF04873 1 - 79 5.8e-36 Ethylene insensitive 3, DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020123)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g21311
prunus_persica Prupe.3G010000_v2.0.a1 Prupe.4G174500_v2.0.a1 Prupe.6G123500_v2.0.a1
pyrus_communis pycom10g09650
rosa_rugosa Rorug02G0245200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 289
AccII CGCG 1 cut(s) 286
AciI CCGC 4 cut(s) 28, 56, 286, 466
AclWI GGATC 2 cut(s) 362, 710
AcsI RAATTY 1 cut(s) 682
AfiI CCNNNNNNNGG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 5 cut(s) 387, 462, 662, 682, 732
AluBI AGCT 3 cut(s) 272, 550, 638
AluI AGCT 3 cut(s) 272, 550, 638
AlwI GGATC 2 cut(s) 362, 710
AoxI GGCC 4 cut(s) 65, 119, 433, 558
ApeKI GCWGC 3 cut(s) 179, 272, 647
ApoI RAATTY 1 cut(s) 682
AseI ATTAAT 1 cut(s) 540
Asp700I GAANNNNTTC 1 cut(s) 236
AspS9I GGNCC 2 cut(s) 119, 586
AsuHPI GGTGA 2 cut(s) 104, 724
AsuII TTCGAA 1 cut(s) 519
AvaII GGWCC 1 cut(s) 586
BaeGI GKGCMC 1 cut(s) 292
BanI GGYRCC 1 cut(s) 289
BbvI GCAGC 3 cut(s) 166, 259, 659
BceAI ACGGC 1 cut(s) 43
BclI TGATCA 3 cut(s) 255, 439, 493
BfmI CTRYAG 1 cut(s) 180
BglI GCCNNNNNGGC 1 cut(s) 64
BisI GCNGC 3 cut(s) 180, 273, 648
BlsI GCNGC 3 cut(s) 181, 274, 649
Bme18I GGWCC 1 cut(s) 586
BmgT120I GGNCC 2 cut(s) 119, 586
BmiI GGNNCC 3 cut(s) 54, 201, 291
BmrI ACTGGG 1 cut(s) 622
BmsI GCATC 2 cut(s) 349, 634
BmuI ACTGGG 1 cut(s) 622
Bpu14I TTCGAA 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 93
Bsa29I ATCGAT 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse1I ACTGG 1 cut(s) 617
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 1 cut(s) 698
BseNI ACTGG 1 cut(s) 617
BseRI GAGGAG 1 cut(s) 114
BseSI GKGCMC 1 cut(s) 292
BseXI GCAGC 3 cut(s) 166, 259, 659
Bsh1236I CGCG 1 cut(s) 286
BshFI GGCC 4 cut(s) 67, 121, 435, 560
BshNI GGYRCC 1 cut(s) 289
BshVI ATCGAT 1 cut(s) 357
BslFI GGGAC 1 cut(s) 752
BslI CCNNNNNNNGG 1 cut(s) 698
BsmFI GGGAC 1 cut(s) 752
BsnI GGCC 4 cut(s) 67, 121, 435, 560
Bsp119I TTCGAA 1 cut(s) 519
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 6 cut(s) 145, 255, 354, 439, 493, 702
BspACI CCGC 4 cut(s) 28, 56, 286, 466
BspANI GGCC 4 cut(s) 67, 121, 435, 560
BspDI ATCGAT 1 cut(s) 357
BspFNI CGCG 1 cut(s) 286
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 3 cut(s) 54, 201, 291
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 2 cut(s) 362, 710
BspT104I TTCGAA 1 cut(s) 519
BspT107I GGYRCC 1 cut(s) 289
BsrI ACTGG 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 6 cut(s) 145, 255, 354, 439, 493, 702
Bst4CI ACNGT 2 cut(s) 11, 392
BstBI TTCGAA 1 cut(s) 519
BstC8I GCNNGC 3 cut(s) 288, 433, 562
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 1 cut(s) 649
BstFNI CGCG 1 cut(s) 286
BstKTI GATC 6 cut(s) 148, 258, 357, 442, 496, 705
BstMBI GATC 6 cut(s) 145, 255, 354, 439, 493, 702
BstMWI GCNNNNNNNGC 3 cut(s) 64, 179, 644
BstNSI RCATGY 2 cut(s) 330, 371
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 1 cut(s) 292
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 3 cut(s) 166, 259, 659
BstX2I RGATCY 1 cut(s) 702
BstYI RGATCY 1 cut(s) 702
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 4 cut(s) 67, 121, 435, 560
BsuTUI ATCGAT 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 1 cut(s) 649
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 7, 189, 397
Cac8I GCNNGC 3 cut(s) 288, 433, 562
CciI TCATGA 1 cut(s) 142
Cfr13I GGNCC 2 cut(s) 119, 586
ClaI ATCGAT 1 cut(s) 357
CviAII CATG 7 cut(s) 116, 143, 327, 368, 437, 673, 742
DpnI GATC 6 cut(s) 147, 257, 356, 441, 495, 704
DpnII GATC 6 cut(s) 145, 255, 354, 439, 493, 702
EciI GGCGGA 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 586
FaeI CATG 7 cut(s) 119, 146, 330, 371, 440, 676, 745
FaqI GGGAC 1 cut(s) 752
FatI CATG 7 cut(s) 115, 142, 326, 367, 436, 672, 741
FauI CCCGC 2 cut(s) 279, 459
FauNDI CATATG 1 cut(s) 195
FbaI TGATCA 3 cut(s) 255, 439, 493
Fnu4HI GCNGC 3 cut(s) 180, 273, 648
FokI GGATG 1 cut(s) 656
Fsp4HI GCNGC 3 cut(s) 180, 273, 648
GluI GCNGC 3 cut(s) 180, 273, 648
HaeIII GGCC 4 cut(s) 67, 121, 435, 560
Hin1II CATG 7 cut(s) 119, 146, 330, 371, 440, 676, 745
HincII GTYRAC 2 cut(s) 222, 475
HindII GTYRAC 2 cut(s) 222, 475
HinfI GANTC 6 cut(s) 458, 509, 516, 658, 715, 722
HpaI GTTAAC 1 cut(s) 222
HphI GGTGA 2 cut(s) 104, 724
Hpy166II GTNNAC 3 cut(s) 95, 222, 475
Hpy188I TCNGA 3 cut(s) 260, 348, 457
Hpy188III TCNNGA 5 cut(s) 110, 143, 513, 692, 719
Hpy8I GTNNAC 3 cut(s) 95, 222, 475
HpyAV CCTTC 2 cut(s) 611, 692
HpyCH4III ACNGT 2 cut(s) 11, 392
HpyCH4V TGCA 4 cut(s) 4, 182, 326, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 179, 644
Hsp92II CATG 7 cut(s) 119, 146, 330, 371, 440, 676, 745
Ksp22I TGATCA 3 cut(s) 255, 439, 493
KspAI GTTAAC 1 cut(s) 222
Kzo9I GATC 6 cut(s) 145, 255, 354, 439, 493, 702
LmnI GCTCC 3 cut(s) 58, 367, 547
Lsp1109I GCAGC 3 cut(s) 166, 259, 659
LweI GCATC 2 cut(s) 349, 634
MalI GATC 6 cut(s) 147, 257, 356, 441, 495, 704
MboI GATC 6 cut(s) 145, 255, 354, 439, 493, 702
MboII GAAGA 5 cut(s) 241, 365, 477, 641, 707
MflI RGATCY 1 cut(s) 702
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 8 cut(s) 205, 236, 349, 395, 632, 682, 732, 760
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 2 cut(s) 18, 267
MnlI CCTC 8 cut(s) 18, 27, 132, 135, 150, 311, 463, 511
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 3 cut(s) 189, 221, 540
MspA1I CMGCKG 1 cut(s) 272
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 3 cut(s) 64, 179, 644
NdeI CATATG 1 cut(s) 195
NdeII GATC 6 cut(s) 145, 255, 354, 439, 493, 702
NlaIII CATG 7 cut(s) 119, 146, 330, 371, 440, 676, 745
NlaIV GGNNCC 3 cut(s) 54, 201, 291
NspI RCATGY 2 cut(s) 330, 371
NspV TTCGAA 1 cut(s) 519
PagI TCATGA 1 cut(s) 142
PciI ACATGT 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 5 cut(s) 458, 509, 516, 658, 715
PkrI GCNGC 3 cut(s) 181, 274, 649
PleI GAGTC 1 cut(s) 716
PpsI GAGTC 1 cut(s) 716
PscI ACATGT 1 cut(s) 367
PshBI ATTAAT 1 cut(s) 540
PsiI TTATAA 1 cut(s) 766
PspN4I GGNNCC 3 cut(s) 54, 201, 291
PspPI GGNCC 2 cut(s) 119, 586
PstI CTGCAG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 702
PvuII CAGCTG 1 cut(s) 272
SaqAI TTAA 3 cut(s) 189, 221, 540
SatI GCNGC 3 cut(s) 180, 273, 648
Sau3AI GATC 6 cut(s) 145, 255, 354, 439, 493, 702
Sau96I GGNCC 2 cut(s) 119, 586
SchI GAGTC 1 cut(s) 716
SduI GDGCHC 1 cut(s) 292
SfaNI GCATC 2 cut(s) 349, 634
SfcI CTRYAG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 519
SinI GGWCC 1 cut(s) 586
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
Sse9I AATT 8 cut(s) 205, 236, 349, 395, 632, 682, 732, 760
SsiI CCGC 4 cut(s) 28, 56, 286, 466
TaaI ACNGT 2 cut(s) 11, 392
TaqI TCGA 2 cut(s) 357, 519
TasI AATT 8 cut(s) 205, 236, 349, 395, 632, 682, 732, 760
TfiI GAWTC 5 cut(s) 458, 509, 516, 658, 715
Tru1I TTAA 3 cut(s) 189, 221, 540
Tru9I TTAA 3 cut(s) 189, 221, 540
TscAI CASTG 3 cut(s) 14, 189, 397
TseI GCWGC 3 cut(s) 179, 272, 647
TspGWI ACGGA 2 cut(s) 24, 87
TspRI CASTG 3 cut(s) 14, 189, 397
VpaK11BI GGWCC 1 cut(s) 586
VspI ATTAAT 1 cut(s) 540
XapI RAATTY 1 cut(s) 682
XceI RCATGY 2 cut(s) 330, 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.