FvH4_6g21970

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017505
Physical Location & Seq
Reverse (-)
15652986 .. 15656666
3681 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21970.t1

Sequence Viewer

Length: 2556 bp
ATGGAAGAAGAAGACCAACAAAAAGTCGCAGATCTGGTCAAGGAGCTCGTCATTCGCCTCGTCGCCGAAAACCCCACCTCTTCTTCATCTCACCCACCAAATCTCCAGACCTCCCTCCGCTACGCGATTCGCATACTCTCCAGCCGATTGACCCCCTCCGTCGCCCCCGACGCCGCCGCCATCGCCGAGTCCACCAAACGCCGCCTCGCCACCCAAGGTAAGTCCTCCCAGGCCCTAACCTTCGCCGATCTTTACACCAAATTCGCATCGAAAACTGGGCCCGGAAGTGTAGATAACAAGTGGGCTGTGCTTTATTTGCTTAAGATTATATCCGAGGATCGAAAGAATGTGAATTCCCAGTTGGATTCTTCGATTTTGTTGCCCAATTTGGGTTTATACGACGCCGAATCGGGCGAAGAGTCTAGGATTTTGGGAGGGAGGGGTAGTGGGGAGAAGGGATGGAGTAATGGGGTTTTGTTGGTTTCCAAGGACCCAGAGAATCTCCGGGAAATTGCGTTTAGGGAGTTTGTTAATTTGGTTAAGGAGGAGAGTGAGGTTTCCGAGGAGGCTTTGGTTAGAGATGTGTTGTATGCTTGTCAGGGAATTGATGGGAAGTATGTGAAATTTGATAGTAATGCGGATGGATATGTTTTGTCCGATTTGGTTAAGGTTCCAAGAGGTACTAGGATTATGGTGAGGAAGCTTTGTGAGTTGGGGTGGTTGTTTAGGAAGGTTAAGGGGTATATATCGGAGAGTATGGATGGGTTTCCGTCTGCAGATGTTGGAACCATTGGGCAGGCATTCTGTGCTGCATTGCAAGATGAGTTGTCGGATTATTATAAGTTATTGGCAGTGCTTGAAGCGCAGGCGATGAATCCGATTCCTTTGGTATCGGAGACAGTGAGCTCTGGGAATTATCTTTCTTTGAGGAGGTTGTCTGTTTGGTTTGCAGAGCCAATGGTGAAAATGAGGTTGATGGCTGTTTTGGTTGATAAGTGTAGGGTTTTGAGGGGTGGGGCGAGGGCTGGGGCTATTCATATGCATGCTCAGCATGGTGACCCTTTAGTGCATGAGTTCATGGGGCGGTTACTCCGTCGGGTGTGCTCCCCACTATTTGAGATGGTCAGGAGTTGGGTTTTGGAAGGAGAGTTGGAAGATGTTTTTGCTGAATTCTTTATCGTGGGTCAGCCAGTGAAAGCTGAATCTCTTTGGAGGGAAGGTTACATGCTCCATGCGGGGATGCTTCCTTCTTTCATTTCACAATCTCTTGCTCAACGCATTCTGAGGACTGGAAAGTCAATCAATTTCCTTCGGGTCTGTTGCGAAGATCTTGGTTGGGCTGATGCTGCAACAGAAGCTGCAGCTGCTGCTGGCACCACAACAAGAAGATGGGGTCTGGGATATGGTGAAACTGATGCTCTTGAGTCTTTGGTCGATGGAGCAGCGAAAAGAGTTGATAAGCATTTGTTGGATGTTATATACAACCAGTATAAATTCAAGGAGCACTGTCTTGCGATAAAGCGGTATTTATTGCTTGGACAGGGTGATTTTGTTCAGTACCTAATGGATATTGTTGGGCCAGAGCTTTCTGAGCCTGCCAACACTATAAGCTCTTTTCAGCTTGCTGGGTTGCTGGAAACTGCAATTCGGTCTTCAAATGCTCAGTATGATGATCGTGACATATTGGATAGATTGAAGGTAAAGATGATGCCACATGAAACTGGAGATAGGGGTTGGGATGTATTCTCATTGGAATATGATGCAAGGGTTCCTTTAGATACTGTTTTTACGGAGTCCGTGATGACAAAGTATTTAAGAATATTTAATTTCTTGTGGAAGCTTCGACGAGTAGAGCATGCCCTTATTGGTGCTTGGAAGACTATGAAACCAAATTGTATCACTTCCCGTTCCTTTATGAAGCTGCAGAAATCAGTTAAGCTGCAGTTGCTTTCAACGCTGAGACGATGCCAGGTTCTTTGGGACGAGATGAATCATTTTGTTTCAAACTTTCAGTACTATATTATGTTTGAAGTTTTGGAGGTGTCATGGTCTAACTTTTTGAACGAAATGGAGGTTGCCAAGGACCTTGATGATCTACTTGCTGCACATGAAAAGTACCTCCATTCAATTGTGGAGAAATCCCTCCTTGGAGAACGTTCCCAGACCCTTTACAGCTCACTCTTTGCCTTGTTAGATCTTATATTGAAATTTCAAAGTCATGCTGATAGATTGTCTGAAGGGATTAATGAGTTGCAAGCAAGAACCATTGAATCCTCTGCACCCTCTCGTGACAAGAGTAAAACAAAAAAGCGATTAAATGATAAATCCTCAGCGCCTGGGTCCTGGATTAGTGATGGCAGGAAGGCCCTGACACAACGTGCTGGTGAATTTCTTCAGAATATGGGGCAGGATCTAGATGCAGTAGCAAAAGAATATTCCTCATTACTTGAGGATTTTATTTCTAAGTTGCCCATGCAGCAACATGTTGATTTAAAGTTCCTCCTATTTCGGCTTGACTTCACTGAATTTTATAGCCAGTTGCATCCTAGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

852

Amino Acids

95.94

Weight (kDa)

6.14

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCP_N_terminal PF17681 192 - 495 9.5e-77 Gamma tubulin complex component N-terminal
GCP_C_terminal PF04130 501 - 844 1.8e-80 Gamma tubulin complex component C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06680
fragaria_vesca FvH4_6g21970
malus_domestica MD12G1055200.v1.1 MD14G1054400.v1.1
prunus_persica Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1
pyrus_communis pycom12g04820 pycom14g04490
rosa_laevigata RLG00000023588
rosa_roxburghii Rroxscaffold_6G00402450
rosa_samantha Rh3CG253200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AasI GACNNNNNNGTC 1 cut(s) 1294
AccB1I GGYRCC 1 cut(s) 1373
AccII CGCG 1 cut(s) 125
AciI CCGC 8 cut(s) 118, 174, 177, 202, 638, 1084, 1235, 1522
AclI AACGTT 1 cut(s) 2155
AclWI GGATC 2 cut(s) 345, 2418
AcsI RAATTY 8 cut(s) 260, 352, 623, 1169, 1493, 2207, 2387, 2525
AcuI CTGAAG 2 cut(s) 2256, 2378
AcyI GRCGYC 2 cut(s) 171, 402
AdeI CACNNNGTG 1 cut(s) 2378
AfaI GTAC 5 cut(s) 682, 1559, 2015, 2117, 2551
AfiI CCNNNNNNNGG 1 cut(s) 389
AflII CTTAAG 1 cut(s) 320
AflIII ACRYGT 1 cut(s) 2482
AjnI CCWGG 4 cut(s) 228, 1968, 2335, 2342
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
Alw21I GWGCWC 4 cut(s) 48, 908, 1106, 1506
Alw26I GTCTC 2 cut(s) 890, 1954
AlwI GGATC 2 cut(s) 345, 2418
AlwNI CAGNNNCTG 3 cut(s) 1358, 1367, 2336
AoxI GGCC 4 cut(s) 231, 278, 1577, 2364
ApaI GGGCCC 1 cut(s) 282
ApoI RAATTY 8 cut(s) 260, 352, 623, 1169, 1493, 2207, 2387, 2525
AseI ATTAAT 1 cut(s) 2244
Asp700I GAANNNNTTC 1 cut(s) 2391
AspLEI GCGC 2 cut(s) 865, 2335
AspS9I GGNCC 8 cut(s) 232, 278, 279, 490, 1577, 2083, 2340, 2365
AsuC2I CCSGG 2 cut(s) 282, 506
AsuHPI GGTGA 7 cut(s) 83, 706, 973, 1067, 1418, 1556, 2396
AvaII GGWCC 3 cut(s) 490, 2083, 2340
BaeGI GKGCMC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 1373
BanII GRGCYC 3 cut(s) 48, 282, 908
BauI CACGAG 1 cut(s) 2286
BbsI GAAGAC 3 cut(s) 18, 1644, 1883
Bbv12I GWGCWC 4 cut(s) 48, 908, 1106, 1506
BbvCI CCTCAGC 1 cut(s) 2329
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BciT130I CCWGG 4 cut(s) 230, 1970, 2337, 2344
BcnI CCSGG 2 cut(s) 282, 506
BcoDI GTCTC 2 cut(s) 890, 1954
BfaI CTAG 4 cut(s) 423, 684, 2414, 2547
BfmI CTRYAG 4 cut(s) 774, 1359, 1922, 1940
BfoI RGCGCY 1 cut(s) 2336
BfrI CTTAAG 1 cut(s) 320
BglII AGATCT 3 cut(s) 31, 1327, 2194
BlpI GCTNAGC 1 cut(s) 1047
BmcAI AGTACT 1 cut(s) 2015
Bme1390I CCNGG 6 cut(s) 230, 282, 506, 1970, 2337, 2344
Bme18I GGWCC 3 cut(s) 490, 2083, 2340
BmgT120I GGNCC 8 cut(s) 232, 278, 279, 490, 1577, 2083, 2340, 2365
BmiI GGNNCC 7 cut(s) 280, 492, 672, 787, 1375, 1770, 2341
BmrFI CCNGG 6 cut(s) 230, 282, 506, 1970, 2337, 2344
BmrI ACTGGG 2 cut(s) 285, 352
BmsI GCATC 9 cut(s) 275, 1230, 1333, 1405, 1698, 1750, 1955, 2407, 2551
BmuI ACTGGG 2 cut(s) 285, 352
BpiI GAAGAC 3 cut(s) 18, 1644, 1883
BpmI CTGGAG 3 cut(s) 89, 124, 1743
Bpu10I CCTNAGC 1 cut(s) 2329
Bpu1102I GCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 2 cut(s) 1442, 2468
BpuMI CCSGG 2 cut(s) 282, 506
BsaAI YACGTR 1 cut(s) 2553
BsaHI GRCGYC 2 cut(s) 171, 402
BsaJI CCNNGG 8 cut(s) 214, 228, 333, 486, 561, 2079, 2146, 2336
BsaXI ACNNNNNCTCC 6 cut(s) 87, 117, 430, 460, 1204, 1234
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 7 cut(s) 280, 358, 1190, 1294, 1486, 1726, 2536
Bse3DI GCAATG 1 cut(s) 812
BseBI CCWGG 4 cut(s) 230, 1970, 2337, 2344
BseDI CCNNGG 8 cut(s) 214, 228, 333, 486, 561, 2079, 2146, 2336
BseGI GGATG 7 cut(s) 464, 646, 766, 1245, 1477, 1744, 2542
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 812
BseMII CTCAG 6 cut(s) 1061, 1274, 1581, 1676, 1949, 2343
BseNI ACTGG 7 cut(s) 280, 358, 1190, 1294, 1486, 1726, 2536
BseRI GAGGAG 3 cut(s) 560, 578, 943
BseSI GKGCMC 1 cut(s) 282
BseYI CCCAGC 2 cut(s) 1025, 1625
BsgI GTGCAG 2 cut(s) 2088, 2262
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 4 cut(s) 233, 280, 1579, 2366
BshNI GGYRCC 1 cut(s) 1373
BsiHKAI GWGCWC 4 cut(s) 48, 908, 1106, 1506
BsiSI CCGG 2 cut(s) 282, 505
BslFI GGGAC 1 cut(s) 1994
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 890, 1954
BsmBI CGTCTC 1 cut(s) 1954
BsmFI GGGAC 1 cut(s) 1994
BsmI GAATGC 2 cut(s) 800, 1278
BsnI GGCC 4 cut(s) 233, 280, 1579, 2366
Bsp120I GGGCCC 1 cut(s) 278
Bsp1286I GDGCHC 5 cut(s) 48, 282, 908, 1106, 1506
Bsp143I GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
Bsp1720I GCTNAGC 1 cut(s) 1047
BspACI CCGC 8 cut(s) 118, 174, 177, 202, 638, 1084, 1235, 1522
BspANI GGCC 4 cut(s) 233, 280, 1579, 2366
BspCNI CTCAG 6 cut(s) 1060, 1275, 1582, 1675, 1950, 2342
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 7 cut(s) 280, 492, 672, 787, 1375, 1770, 2341
BspMAI CTGCAG 4 cut(s) 778, 1363, 1926, 1944
BspPI GGATC 2 cut(s) 345, 2418
BspT107I GGYRCC 1 cut(s) 1373
BspTI CTTAAG 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 812
BsrI ACTGG 7 cut(s) 280, 358, 1190, 1294, 1486, 1726, 2536
BssECI CCNNGG 8 cut(s) 214, 228, 333, 486, 561, 2079, 2146, 2336
BssMI GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
BssNI GRCGYC 2 cut(s) 171, 402
BssSI CACGAG 1 cut(s) 2286
BssT1I CCWWGG 4 cut(s) 214, 486, 2079, 2146
Bst2BI CACGAG 1 cut(s) 2286
Bst2UI CCWGG 4 cut(s) 230, 1970, 2337, 2344
Bst4CI ACNGT 3 cut(s) 901, 1508, 1783
Bst6I CTCTTC 2 cut(s) 85, 411
BstACI GRCGYC 2 cut(s) 171, 402
BstAFI CTTAAG 1 cut(s) 320
BstAPI GCANNNNNTGC 2 cut(s) 806, 1367
BstBAI YACGTR 1 cut(s) 2553
BstC8I GCNNGC 8 cut(s) 798, 867, 1044, 1372, 1596, 1623, 1857, 2256
BstDEI CTNAG 7 cut(s) 1047, 1283, 1590, 1662, 1958, 2329, 2463
BstEII GGTNACC 1 cut(s) 1055
BstF5I GGATG 7 cut(s) 464, 646, 766, 1245, 1477, 1744, 2542
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 2336
BstHHI GCGC 2 cut(s) 865, 2335
BstKTI GATC 8 cut(s) 34, 250, 340, 1330, 1675, 2095, 2197, 2413
BstMAI GTCTC 2 cut(s) 890, 1954
BstMBI GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
BstNI CCWGG 4 cut(s) 230, 1970, 2337, 2344
BstNSI RCATGY 4 cut(s) 1046, 1228, 1859, 2486
BstPI GGTNACC 1 cut(s) 1055
BstSCI CCNGG 6 cut(s) 228, 280, 504, 1968, 2335, 2342
BstSFI CTRYAG 4 cut(s) 774, 1359, 1922, 1940
BstSLI GKGCMC 1 cut(s) 282
BstSNI TACGTA 1 cut(s) 2553
BstUI CGCG 1 cut(s) 125
BstV2I GAAGAC 3 cut(s) 18, 1644, 1883
BstX2I RGATCY 4 cut(s) 31, 1327, 2194, 2410
BstYI RGATCY 4 cut(s) 31, 1327, 2194, 2410
BsuRI GGCC 4 cut(s) 233, 280, 1579, 2366
BtgZI GCGATG 2 cut(s) 166, 884
BtsCI GGATG 7 cut(s) 464, 646, 766, 1245, 1477, 1744, 2542
BtsI GCAGTG 1 cut(s) 858
BtsIMutI CAGTG 5 cut(s) 858, 906, 1197, 1504, 2520
Cac8I GCNNGC 8 cut(s) 798, 867, 1044, 1372, 1596, 1623, 1857, 2256
CaiI CAGNNNCTG 3 cut(s) 1358, 1367, 2336
CfoI GCGC 2 cut(s) 865, 2335
Cfr13I GGNCC 8 cut(s) 232, 278, 279, 490, 1577, 2083, 2340, 2365
CseI GACGC 2 cut(s) 179, 410
Csp6I GTAC 5 cut(s) 681, 1558, 2014, 2116, 2550
CviQI GTAC 5 cut(s) 681, 1558, 2014, 2116, 2550
DdeI CTNAG 7 cut(s) 1047, 1283, 1590, 1662, 1958, 2329, 2463
DpnI GATC 8 cut(s) 33, 249, 339, 1329, 1674, 2094, 2196, 2412
DpnII GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
DraI TTTAAA 1 cut(s) 2493
DraIII CACNNNGTG 1 cut(s) 2378
DrdI GACNNNNNNGTC 1 cut(s) 1294
DseDI GACNNNNNNGTC 1 cut(s) 1294
Eam1104I CTCTTC 2 cut(s) 85, 411
EarI CTCTTC 2 cut(s) 85, 411
Ecl136II GAGCTC 2 cut(s) 46, 906
Eco105I TACGTA 1 cut(s) 2553
Eco130I CCWWGG 4 cut(s) 214, 486, 2079, 2146
Eco24I GRGCYC 3 cut(s) 48, 282, 908
Eco47I GGWCC 3 cut(s) 490, 2083, 2340
Eco53kI GAGCTC 2 cut(s) 46, 906
Eco57I CTGAAG 2 cut(s) 2256, 2378
Eco91I GGTNACC 1 cut(s) 1055
EcoICRI GAGCTC 2 cut(s) 46, 906
EcoO109I RGGNCCY 5 cut(s) 232, 490, 2083, 2340, 2365
EcoO65I GGTNACC 1 cut(s) 1055
EcoRI GAATTC 2 cut(s) 352, 1169
EcoRII CCWGG 4 cut(s) 228, 1968, 2335, 2342
EcoT14I CCWWGG 4 cut(s) 214, 486, 2079, 2146
EcoT22I ATGCAT 1 cut(s) 1044
EcoT38I GRGCYC 3 cut(s) 48, 282, 908
ErhI CCWWGG 4 cut(s) 214, 486, 2079, 2146
Esp3I CGTCTC 1 cut(s) 1954
FalI AAGNNNNNCTT 2 cut(s) 1756, 1788
FaqI GGGAC 1 cut(s) 1994
FauI CCCGC 1 cut(s) 1228
FauNDI CATATG 1 cut(s) 1038
FokI GGATG 7 cut(s) 471, 653, 773, 1252, 1484, 1751, 2529
FriOI GRGCYC 3 cut(s) 48, 282, 908
FspBI CTAG 4 cut(s) 423, 684, 2414, 2547
GlaI GCGC 2 cut(s) 864, 2334
GsaI CCCAGC 2 cut(s) 1029, 1629
GsuI CTGGAG 3 cut(s) 89, 124, 1743
HaeII RGCGCY 1 cut(s) 2336
HaeIII GGCC 4 cut(s) 233, 280, 1579, 2366
HapII CCGG 2 cut(s) 282, 505
HgaI GACGC 2 cut(s) 179, 410
HhaI GCGC 2 cut(s) 865, 2335
Hin1I GRCGYC 2 cut(s) 171, 402
Hin6I GCGC 2 cut(s) 863, 2333
HinP1I GCGC 2 cut(s) 863, 2333
HindIII AAGCTT 2 cut(s) 701, 1838
HpaII CCGG 2 cut(s) 282, 505
HphI GGTGA 7 cut(s) 83, 706, 973, 1067, 1418, 1556, 2396
Hpy166II GTNNAC 1 cut(s) 192
Hpy188III TCNNGA 6 cut(s) 106, 1126, 1421, 1676, 2288, 2414
Hpy8I GTNNAC 1 cut(s) 192
Hpy99I CGWCG 6 cut(s) 65, 164, 173, 404, 1098, 1848
HpyCH4III ACNGT 3 cut(s) 901, 1508, 1783
HpyCH4IV ACGT 3 cut(s) 2155, 2377, 2552
HpyF3I CTNAG 7 cut(s) 1047, 1283, 1590, 1662, 1958, 2329, 2463
HpySE526I ACGT 3 cut(s) 2155, 2377, 2552
Hsp92I GRCGYC 2 cut(s) 171, 402
HspAI GCGC 2 cut(s) 863, 2333
Kzo9I GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
LmnI GCTCC 5 cut(s) 43, 1109, 1233, 1439, 1501
LweI GCATC 9 cut(s) 275, 1230, 1333, 1405, 1698, 1750, 1955, 2407, 2551
MaeI CTAG 4 cut(s) 423, 684, 2414, 2547
MaeII ACGT 3 cut(s) 2155, 2377, 2552
MaeIII GTNAC 5 cut(s) 1055, 1086, 1220, 1676, 2288
MalI GATC 8 cut(s) 33, 249, 339, 1329, 1674, 2094, 2196, 2412
MboI GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
MfeI CAATTG 1 cut(s) 2127
MflI RGATCY 4 cut(s) 31, 1327, 2194, 2410
MhlI GDGCHC 5 cut(s) 48, 282, 908, 1106, 1506
MlyI GAGTC 4 cut(s) 197, 428, 1433, 1802
MmeI TCCRAC 5 cut(s) 342, 763, 810, 1131, 1449
Mph1103I ATGCAT 1 cut(s) 1044
MroXI GAANNNNTTC 1 cut(s) 2391
MslI CAYNNNNRTG 1 cut(s) 1041
MspA1I CMGCKG 1 cut(s) 1364
MspCI CTTAAG 1 cut(s) 320
MspI CCGG 2 cut(s) 282, 505
MspR9I CCNGG 6 cut(s) 230, 282, 506, 1970, 2337, 2344
MunI CAATTG 1 cut(s) 2127
Mva1269I GAATGC 2 cut(s) 800, 1278
MvaI CCWGG 4 cut(s) 230, 1970, 2337, 2344
MvnI CGCG 1 cut(s) 125
NciI CCSGG 2 cut(s) 282, 506
NdeI CATATG 1 cut(s) 1038
NdeII GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
NlaIV GGNNCC 7 cut(s) 280, 492, 672, 787, 1375, 1770, 2341
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 3 cut(s) 1055, 1676, 2288
NsiI ATGCAT 1 cut(s) 1044
NspI RCATGY 4 cut(s) 1046, 1228, 1859, 2486
PaeI GCATGC 2 cut(s) 1046, 1859
PciI ACATGT 1 cut(s) 2482
PctI GAATGC 2 cut(s) 800, 1278
PdmI GAANNNNTTC 1 cut(s) 2391
PfeI GAWTC 9 cut(s) 127, 365, 407, 499, 874, 880, 1202, 1990, 2270
PfoI TCCNGGA 2 cut(s) 504, 2342
PleI GAGTC 4 cut(s) 196, 427, 1432, 1801
PpsI GAGTC 4 cut(s) 196, 427, 1432, 1801
Ppu21I YACGTR 1 cut(s) 2553
PpuMI RGGWCCY 3 cut(s) 490, 2083, 2340
PscI ACATGT 1 cut(s) 2482
PshBI ATTAAT 1 cut(s) 2244
PsiI TTATAA 1 cut(s) 840
Psp124BI GAGCTC 2 cut(s) 48, 908
Psp1406I AACGTT 1 cut(s) 2155
Psp5II RGGWCCY 3 cut(s) 490, 2083, 2340
Psp6I CCWGG 4 cut(s) 228, 1968, 2335, 2342
PspEI GGTNACC 1 cut(s) 1055
PspFI CCCAGC 2 cut(s) 1025, 1625
PspGI CCWGG 4 cut(s) 228, 1968, 2335, 2342
PspN4I GGNNCC 7 cut(s) 280, 492, 672, 787, 1375, 1770, 2341
PspOMI GGGCCC 1 cut(s) 278
PspPI GGNCC 8 cut(s) 232, 278, 279, 490, 1577, 2083, 2340, 2365
PspPPI RGGWCCY 3 cut(s) 490, 2083, 2340
PstI CTGCAG 4 cut(s) 778, 1363, 1926, 1944
PstNI CAGNNNCTG 3 cut(s) 1358, 1367, 2336
PsuI RGATCY 4 cut(s) 31, 1327, 2194, 2410
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 5 cut(s) 682, 1559, 2015, 2117, 2551
RsaNI GTAC 5 cut(s) 681, 1558, 2014, 2116, 2550
RseI CAYNNNNRTG 1 cut(s) 1041
SacI GAGCTC 2 cut(s) 48, 908
Sau3AI GATC 8 cut(s) 31, 247, 337, 1327, 1672, 2092, 2194, 2410
Sau96I GGNCC 8 cut(s) 232, 278, 279, 490, 1577, 2083, 2340, 2365
ScaI AGTACT 1 cut(s) 2015
SchI GAGTC 4 cut(s) 197, 428, 1433, 1802
ScrFI CCNGG 6 cut(s) 230, 282, 506, 1970, 2337, 2344
SduI GDGCHC 5 cut(s) 48, 282, 908, 1106, 1506
SfaNI GCATC 9 cut(s) 275, 1230, 1333, 1405, 1698, 1750, 1955, 2407, 2551
SfcI CTRYAG 4 cut(s) 774, 1359, 1922, 1940
SinI GGWCC 3 cut(s) 490, 2083, 2340
SmiMI CAYNNNNRTG 1 cut(s) 1041
SmlI CTYRAG 3 cut(s) 320, 1421, 2447
SmoI CTYRAG 3 cut(s) 320, 1421, 2447
SnaBI TACGTA 1 cut(s) 2553
SphI GCATGC 2 cut(s) 1046, 1859
SsiI CCGC 8 cut(s) 118, 174, 177, 202, 638, 1084, 1235, 1522
SspI AATATT 2 cut(s) 1821, 2435
SspMI CTAG 4 cut(s) 423, 684, 2414, 2547
SstI GAGCTC 2 cut(s) 48, 908
StyD4I CCNGG 6 cut(s) 228, 280, 504, 1968, 2335, 2342
StyI CCWWGG 4 cut(s) 214, 486, 2079, 2146
TaaI ACNGT 3 cut(s) 901, 1508, 1783
TaiI ACGT 3 cut(s) 2158, 2380, 2555
TaqI TCGA 5 cut(s) 269, 340, 371, 1434, 1843
TaqII GACCGA 1 cut(s) 1638
TatI WGTACW 1 cut(s) 2013
TauI GCSGC 3 cut(s) 176, 179, 204
TfiI GAWTC 9 cut(s) 127, 365, 407, 499, 874, 880, 1202, 1990, 2270
TscAI CASTG 5 cut(s) 858, 906, 1197, 1511, 2527
TseFI GTSAC 3 cut(s) 1055, 1676, 2288
Tsp45I GTSAC 3 cut(s) 1055, 1676, 2288
TspGWI ACGGA 5 cut(s) 148, 759, 1082, 1786, 1805
TspRI CASTG 5 cut(s) 858, 906, 1197, 1511, 2527
Vha464I CTTAAG 1 cut(s) 320
VpaK11BI GGWCC 3 cut(s) 490, 2083, 2340
VspI ATTAAT 1 cut(s) 2244
XapI RAATTY 8 cut(s) 260, 352, 623, 1169, 1493, 2207, 2387, 2525
XbaI TCTAGA 1 cut(s) 2413
XceI RCATGY 4 cut(s) 1046, 1228, 1859, 2486
XmnI GAANNNNTTC 1 cut(s) 2391
XspI CTAG 4 cut(s) 423, 684, 2414, 2547
ZrmI AGTACT 1 cut(s) 2015
Zsp2I ATGCAT 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.