Rroxscaffold_6G00402450

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017505
Physical Location & Seq
Reverse (-)
24917564 .. 24922273
4710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402450.1

Sequence Viewer

Length: 2553 bp
ATGGAAGAAGAAGACCAGCAAAAAGTCGCAGATCTGGTCAAGGAGCTCGTCATTCGCTTGGTCGCCCAAAACTCCACCTCCGAATCTCACCCACCAAATCCCAACTCCCCTCATCTCCAAACCTCACTTCGCTACGCGATTCGCTTACTCTCCAGCCGATTGACTCCCTCCGTCGCCCCCGACGCCGCCGCCATCGCCGAGTCCACCAAACGCCGACTCGCCACTCAAGGTAAGTCCTCGCAAGCCCTAACTTTCGCCGACCTTTATACTAGGTTCGCGTCCAAAACCGGGCCCGGAAGTGTAAATAACAAGTGGGCGGTTATCTATTTGCTTAAGATTATATCGGAGGATCGAAAGAGTGTGAATTCCCAGTTGGATTCTTCGGTTTTGTTGCCCAATTTGGCTTTAAACGACGCCGGATCGGGCGACGAGTCGAGGGGTTTGGGGGGAAGGGGAAGTGGGGAAAAGGGTTGGAGCAATGGGGTTTTGTTAGTTTCCAAGGACCCGGAGAATCTCCGGGAAATTGCGTTTAGGGAGTTTGTGAATTTGGTTAAGGAAGAGAATGAAGTTTCCGAAGAGGCTTTGGTGAGAGATGTGTTGTATGCTTGTCAGGGAATTGATGGGAAGTATGTGAAATTCGACAGTAATGCGGATGGCTATGTTTTGTCCGATTTGATAAAGGTTCCGAGAGCTACCAGGATTATGGTTCGGAAGCTTTGTGAATTGGGGTGGTTGTTTAGGAAGGTTAAGGGGTATATCTCGGAGAGTATGGATGGGTTTCCATCCGAAGATGTTGGAACCATTGGGCAGGCTTTTTGTGCTGCATTGCAAGATGAGCTCTCCGATTATTATAAGTTGTTGGCGGTGCTTGAAGCACAGGCGATGAATCCGATTCCCTTGGTTTCAGAGACAGTGAGCTCGGGGAATTATCTTTCTATGAGGAGGTTGTCTGTTTGGTTTGCAGAGCCAATGGTGAAAATGAGGTTGATGGCTGTTTTGGTTGATAAATGTAGGGTTTTAAGGGGTGGGGCAATGGCTGGGGCTATTCATATGCACGCTCAGCATGGGGACCCACTAGTGCATGAGTTCATGGGGCGGTTACTCCGTCGGGTGTGCTCCCCACTGTTTGAGATGGTCAGGAGTTGGGTTTTGGAAGGGGAGTTAGAAGATGTTTTTGCTGAATTCTTTATTGTGGGTCAGCCGGTGAAAGCTGAATCTCTTTGGAGGGAAGGTTACATGCTCCATGCTGGGATGCTTCCTTCTTTTATTTCACAATCTCTTGCTCAACGCATTCTGAGGACTGGAAAGTCGATTAATTTCCTTCGCGTCTGTTGTGACGATCGTGGTTGGGCAGATGCTGCACTAGAAGCTGCAGCTGCTGCTGGCACTACGACAAGAAGATGGGGTCTTGGATATGGTGAAACTGATGCTCTTGAGTCTTTGGTCGATGGAGCAGCAAAGAGAGTTGATAAGCATTTGTTGGATGTTATATACAACCAGTATAAGTTCAAGGAGCACTGTCTTGCAATAAAGCGGTATTTACTGCTTGGACAGGGTGACTTTGTTCAGTACCTAATGGATATTGTTGGGCCAGAGCTTTCTGAGCCTGCTAACACTATAAGCTCTTTCCAGCTAGCTGGGTTGCTGGAAACTGCAATCCGATCGTCTAATGCACAATATGATGATCGCGACATATTGGATAGATTGAAGGTAAAGATGATGCCACATGAAACTGGAGACAGAGGTTGGGATGTATTCTCATTGGAATATGATGCAAGGGTTCCTTTAGATACTGTTTTTACGGAATCTGTGATGACAAAGTATTTAAGAATATTTAATTTCTTGTGGAAGCTTCGACGAGTAGAGCATGCTCTTATTGGTGCTTGGAAGACTATGAAACCAAATTGTATCACTGCCCGTTCCTTCATGAAGCTGCAGAATGCAGTTAAGTTGCCGTTGCTTTCAACTCTGAGACGATGCCAGGTTCTTTGGGATGAGATGAATCATTTTGTTTCAAACTTGCAGTACTATATTATGTTTGAAGTCTTGGAGGTGTCATGGTCTGACTTTTTGAACGAAATGGAGGTGGCCAAGGACCTTGATGATCTACTTGCTGCACATGAAAAGTACCTCCATTCAATTGTGGAGAAATCCCTCCTTGGAGAACGTTCCCAAACCCTTTACAGCTCACTCTTTGCCTTGTTTGATCTTATATTGAAATTTCGAAGTCATGCTGATAGATTGTCTGAAGGGATCAATGAGTTGCAAGCAAGGAAGGCCCTCACACAACGTGCTGGCGAATTTCTTCGGAATATGGGACAGGATCTAGATGCAGTAGCAAAAGAATATTCGTCATTGCTTGAGGATTTTATTTCTAAGTTGCCCATGCAGCAACATGTTGATTTAAAGTTCCTCCTGTTTCGGCTTGACTTCACCGAATTTTACAGCCAGTTGCGTCCTAATGGTGTTTGCTTCATTCTGATGGGCATGTGTGGAAGTTTATGGTTTGACTTGAAGATGTATTTCATCAGTAGATCAGGGTGTGAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

850

Amino Acids

96.04

Weight (kDa)

5.99

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCP_N_terminal PF17681 196 - 499 1.3e-77 Gamma tubulin complex component N-terminal
GCP_C_terminal PF04130 505 - 815 2.1e-81 Gamma tubulin complex component C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06680
fragaria_vesca FvH4_6g21970
malus_domestica MD12G1055200.v1.1 MD14G1054400.v1.1
prunus_persica Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1
pyrus_communis pycom12g04820 pycom14g04490
rosa_laevigata RLG00000023588
rosa_roxburghii Rroxscaffold_6G00402450
rosa_samantha Rh3CG253200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
AasI GACNNNNNNGTC 1 cut(s) 1306
AccII CGCG 4 cut(s) 137, 278, 1326, 1689
AciI CCGC 7 cut(s) 186, 189, 317, 650, 863, 1096, 1534
AclI AACGTT 1 cut(s) 2167
AclWI GGATC 4 cut(s) 357, 427, 2261, 2331
AcoI YGGCCR 1 cut(s) 2088
AcsI RAATTY 7 cut(s) 364, 544, 635, 1181, 2219, 2300, 2438
AcuI CTGAAG 1 cut(s) 2268
AcyI GRCGYC 2 cut(s) 183, 414
AdeI CACNNNGTG 1 cut(s) 2291
AfaI GTAC 3 cut(s) 1571, 2027, 2129
AfiI CCNNNNNNNGG 1 cut(s) 288
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 2395
AhlI ACTAGT 1 cut(s) 1075
AjnI CCWGG 2 cut(s) 695, 1980
AjuI GAANNNNNNNTTGG 4 cut(s) 111, 143, 356, 388
Alw21I GWGCWC 5 cut(s) 48, 840, 920, 1118, 1518
Alw26I GTCTC 3 cut(s) 902, 1731, 1966
AlwI GGATC 4 cut(s) 357, 427, 2261, 2331
AlwNI CAGNNNCTG 2 cut(s) 1358, 1379
Ama87I CYCGRG 1 cut(s) 919
AoxI GGCC 4 cut(s) 290, 1589, 2088, 2277
ApaI GGGCCC 1 cut(s) 294
ApoI RAATTY 7 cut(s) 364, 544, 635, 1181, 2219, 2300, 2438
AseI ATTAAT 1 cut(s) 1314
Asp700I GAANNNNTTC 1 cut(s) 2304
AspS9I GGNCC 7 cut(s) 290, 291, 502, 1069, 1589, 2095, 2278
AsuC2I CCSGG 4 cut(s) 289, 294, 506, 518
AsuHPI GGTGA 7 cut(s) 80, 598, 985, 1216, 1430, 1568, 2425
AsuII TTCGAA 1 cut(s) 2224
AsuNHI GCTAGC 1 cut(s) 1633
AvaI CYCGRG 1 cut(s) 919
AvaII GGWCC 3 cut(s) 502, 1069, 2095
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 2090
BanII GRGCYC 4 cut(s) 48, 294, 840, 920
BbsI GAAGAC 2 cut(s) 18, 1895
Bbv12I GWGCWC 5 cut(s) 48, 840, 920, 1118, 1518
BceAI ACGGC 1 cut(s) 1939
BcgI CGANNNNNNTGC 4 cut(s) 629, 663, 1644, 1678
BciT130I CCWGG 2 cut(s) 697, 1982
BcnI CCSGG 4 cut(s) 289, 294, 506, 518
BcoDI GTCTC 3 cut(s) 902, 1731, 1966
BcuI ACTAGT 1 cut(s) 1075
BfaI CTAG 5 cut(s) 270, 1076, 1364, 1634, 2327
BfmI CTRYAG 2 cut(s) 1371, 1934
BfrI CTTAAG 1 cut(s) 332
BglII AGATCT 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 1059
BmcAI AGTACT 1 cut(s) 2027
Bme1390I CCNGG 6 cut(s) 289, 294, 506, 518, 697, 1982
Bme18I GGWCC 3 cut(s) 502, 1069, 2095
BmeT110I CYCGRG 1 cut(s) 919
BmgT120I GGNCC 7 cut(s) 290, 291, 502, 1069, 1589, 2095, 2278
BmiI GGNNCC 7 cut(s) 292, 504, 684, 799, 1070, 1071, 1782
BmrFI CCNGG 6 cut(s) 289, 294, 506, 518, 697, 1982
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 7 cut(s) 1242, 1345, 1417, 1710, 1762, 1967, 2320
BmtI GCTAGC 1 cut(s) 1637
BmuI ACTGGG 1 cut(s) 364
BpiI GAAGAC 2 cut(s) 18, 1895
BpmI CTGGAG 2 cut(s) 136, 1755
Bpu1102I GCTNAGC 1 cut(s) 1059
Bpu14I TTCGAA 1 cut(s) 2224
BpuEI CTTGAG 3 cut(s) 210, 1454, 2381
BpuMI CCSGG 4 cut(s) 289, 294, 506, 518
BsaHI GRCGYC 2 cut(s) 183, 414
BsaJI CCNNGG 4 cut(s) 498, 897, 2091, 2158
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 1216, 1246
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse118I RCCGGY 1 cut(s) 1201
Bse1I ACTGG 5 cut(s) 370, 1306, 1498, 1738, 2449
Bse3DI GCAATG 4 cut(s) 484, 824, 1038, 2354
BseBI CCWGG 2 cut(s) 697, 1982
BseDI CCNNGG 4 cut(s) 498, 897, 2091, 2158
BseGI GGATG 7 cut(s) 658, 778, 782, 1257, 1489, 1756, 1999
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMI GCAATG 4 cut(s) 484, 824, 1038, 2354
BseMII CTCAG 4 cut(s) 1073, 1286, 1593, 1961
BseNI ACTGG 5 cut(s) 370, 1306, 1498, 1738, 2449
BseRI GAGGAG 1 cut(s) 955
BseSI GKGCMC 1 cut(s) 294
BseYI CCCAGC 3 cut(s) 1037, 1247, 1637
BsgI GTGCAG 2 cut(s) 1344, 2100
Bsh1236I CGCG 4 cut(s) 137, 278, 1326, 1689
Bsh1285I CGRYCG 2 cut(s) 1342, 1664
BshFI GGCC 4 cut(s) 292, 1591, 2090, 2279
BsiEI CGRYCG 2 cut(s) 1342, 1664
BsiHKAI GWGCWC 5 cut(s) 48, 840, 920, 1118, 1518
BsiHKCI CYCGRG 1 cut(s) 919
BsiSI CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
BslFI GGGAC 2 cut(s) 1082, 2331
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 3 cut(s) 902, 1731, 1966
BsmBI CGTCTC 1 cut(s) 1966
BsmFI GGGAC 2 cut(s) 1082, 2331
BsmI GAATGC 2 cut(s) 1290, 1945
BsnI GGCC 4 cut(s) 292, 1591, 2090, 2279
BsoBI CYCGRG 1 cut(s) 919
Bsp119I TTCGAA 1 cut(s) 2224
Bsp120I GGGCCC 1 cut(s) 290
Bsp1286I GDGCHC 6 cut(s) 48, 294, 840, 920, 1118, 1518
Bsp1720I GCTNAGC 1 cut(s) 1059
Bsp68I TCGCGA 1 cut(s) 1689
BspACI CCGC 7 cut(s) 186, 189, 317, 650, 863, 1096, 1534
BspANI GGCC 4 cut(s) 292, 1591, 2090, 2279
BspCNI CTCAG 4 cut(s) 1072, 1287, 1594, 1962
BspFNI CGCG 4 cut(s) 137, 278, 1326, 1689
BspHI TCATGA 1 cut(s) 1926
BspLI GGNNCC 7 cut(s) 292, 504, 684, 799, 1070, 1071, 1782
BspMAI CTGCAG 2 cut(s) 1375, 1938
BspOI GCTAGC 1 cut(s) 1637
BspPI GGATC 4 cut(s) 357, 427, 2261, 2331
BspT104I TTCGAA 1 cut(s) 2224
BspTI CTTAAG 1 cut(s) 332
BsrDI GCAATG 4 cut(s) 484, 824, 1038, 2354
BsrFI RCCGGY 1 cut(s) 1201
BsrI ACTGG 5 cut(s) 370, 1306, 1498, 1738, 2449
BssAI RCCGGY 1 cut(s) 1201
BssECI CCNNGG 4 cut(s) 498, 897, 2091, 2158
BssNI GRCGYC 2 cut(s) 183, 414
BssT1I CCWWGG 4 cut(s) 498, 897, 2091, 2158
Bst2UI CCWGG 2 cut(s) 697, 1982
Bst4CI ACNGT 5 cut(s) 644, 913, 1125, 1520, 1795
Bst6I CTCTTC 2 cut(s) 552, 570
BstACI GRCGYC 2 cut(s) 183, 414
BstAFI CTTAAG 1 cut(s) 332
BstAPI GCANNNNNTGC 2 cut(s) 1358, 1379
BstBI TTCGAA 1 cut(s) 2224
BstC8I GCNNGC 9 cut(s) 243, 810, 1056, 1384, 1608, 1635, 1869, 2268, 2296
BstDEI CTNAG 5 cut(s) 1059, 1295, 1602, 1970, 2376
BstF5I GGATG 7 cut(s) 658, 778, 782, 1257, 1489, 1756, 1999
BstFNI CGCG 4 cut(s) 137, 278, 1326, 1689
BstMAI GTCTC 3 cut(s) 902, 1731, 1966
BstMCI CGRYCG 2 cut(s) 1342, 1664
BstNI CCWGG 2 cut(s) 697, 1982
BstNSI RCATGY 4 cut(s) 1240, 1871, 2399, 2491
BstSCI CCNGG 6 cut(s) 287, 292, 504, 516, 695, 1980
BstSFI CTRYAG 2 cut(s) 1371, 1934
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 4 cut(s) 137, 278, 1326, 1689
BstV2I GAAGAC 2 cut(s) 18, 1895
BstX2I RGATCY 2 cut(s) 31, 2323
BstXI CCANNNNNNTGG 2 cut(s) 703, 1637
BstYI RGATCY 2 cut(s) 31, 2323
BsuRI GGCC 4 cut(s) 292, 1591, 2090, 2279
BtgZI GCGATG 2 cut(s) 178, 896
BtsCI GGATG 7 cut(s) 658, 778, 782, 1257, 1489, 1756, 1999
BtsI GCAGTG 1 cut(s) 1911
BtsIMutI CAGTG 4 cut(s) 918, 1121, 1516, 1911
BtuMI TCGCGA 1 cut(s) 1689
Cac8I GCNNGC 9 cut(s) 243, 810, 1056, 1384, 1608, 1635, 1869, 2268, 2296
CaiI CAGNNNCTG 2 cut(s) 1358, 1379
CciI TCATGA 1 cut(s) 1926
Cfr10I RCCGGY 1 cut(s) 1201
Cfr13I GGNCC 7 cut(s) 290, 291, 502, 1069, 1589, 2095, 2278
CseI GACGC 5 cut(s) 191, 267, 422, 1315, 2444
Csp6I GTAC 3 cut(s) 1570, 2026, 2128
CviQI GTAC 3 cut(s) 1570, 2026, 2128
DdeI CTNAG 5 cut(s) 1059, 1295, 1602, 1970, 2376
DraI TTTAAA 2 cut(s) 408, 2406
DraIII CACNNNGTG 1 cut(s) 2291
DrdI GACNNNNNNGTC 1 cut(s) 1306
DseDI GACNNNNNNGTC 1 cut(s) 1306
EaeI YGGCCR 1 cut(s) 2088
Eam1104I CTCTTC 2 cut(s) 552, 570
EarI CTCTTC 2 cut(s) 552, 570
Ecl136II GAGCTC 3 cut(s) 46, 838, 918
Eco130I CCWWGG 4 cut(s) 498, 897, 2091, 2158
Eco24I GRGCYC 4 cut(s) 48, 294, 840, 920
Eco47I GGWCC 3 cut(s) 502, 1069, 2095
Eco53kI GAGCTC 3 cut(s) 46, 838, 918
Eco57I CTGAAG 1 cut(s) 2268
Eco88I CYCGRG 1 cut(s) 919
EcoICRI GAGCTC 3 cut(s) 46, 838, 918
EcoO109I RGGNCCY 4 cut(s) 502, 1069, 2095, 2278
EcoRI GAATTC 2 cut(s) 364, 1181
EcoRII CCWGG 2 cut(s) 695, 1980
EcoT14I CCWWGG 4 cut(s) 498, 897, 2091, 2158
EcoT38I GRGCYC 4 cut(s) 48, 294, 840, 920
ErhI CCWWGG 4 cut(s) 498, 897, 2091, 2158
Esp3I CGTCTC 1 cut(s) 1966
FalI AAGNNNNNCTT 2 cut(s) 1768, 1800
FaqI GGGAC 2 cut(s) 1082, 2331
FauNDI CATATG 1 cut(s) 1050
FokI GGATG 7 cut(s) 665, 769, 785, 1264, 1496, 1763, 2006
FriOI GRGCYC 4 cut(s) 48, 294, 840, 920
FspBI CTAG 5 cut(s) 270, 1076, 1364, 1634, 2327
GsaI CCCAGC 3 cut(s) 1041, 1251, 1641
GsuI CTGGAG 2 cut(s) 136, 1755
HaeIII GGCC 4 cut(s) 292, 1591, 2090, 2279
HapII CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
HgaI GACGC 5 cut(s) 191, 267, 422, 1315, 2444
Hin1I GRCGYC 2 cut(s) 183, 414
HindIII AAGCTT 2 cut(s) 713, 1850
HpaII CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
HphI GGTGA 7 cut(s) 80, 598, 985, 1216, 1430, 1568, 2425
Hpy166II GTNNAC 1 cut(s) 204
Hpy188III TCNNGA 5 cut(s) 1138, 1433, 1688, 1927, 2327
Hpy8I GTNNAC 1 cut(s) 204
Hpy99I CGWCG 6 cut(s) 176, 185, 416, 431, 1110, 1860
HpyCH4III ACNGT 5 cut(s) 644, 913, 1125, 1520, 1795
HpyCH4IV ACGT 2 cut(s) 2167, 2290
HpyF3I CTNAG 5 cut(s) 1059, 1295, 1602, 1970, 2376
HpySE526I ACGT 2 cut(s) 2167, 2290
Hsp92I GRCGYC 2 cut(s) 183, 414
KflI GGGWCCC 1 cut(s) 1069
LmnI GCTCC 6 cut(s) 43, 474, 1121, 1245, 1451, 1513
LweI GCATC 7 cut(s) 1242, 1345, 1417, 1710, 1762, 1967, 2320
MaeI CTAG 5 cut(s) 270, 1076, 1364, 1634, 2327
MaeII ACGT 2 cut(s) 2167, 2290
MaeIII GTNAC 4 cut(s) 1098, 1232, 1334, 1556
MfeI CAATTG 1 cut(s) 2139
MflI RGATCY 2 cut(s) 31, 2323
MhlI GDGCHC 6 cut(s) 48, 294, 840, 920, 1118, 1518
MlsI TGGCCA 1 cut(s) 2090
MluNI TGGCCA 1 cut(s) 2090
MlyI GAGTC 5 cut(s) 157, 209, 210, 440, 1445
MmeI TCCRAC 4 cut(s) 354, 452, 775, 1461
Mox20I TGGCCA 1 cut(s) 2090
MroXI GAANNNNTTC 1 cut(s) 2304
MscI TGGCCA 1 cut(s) 2090
MslI CAYNNNNRTG 1 cut(s) 2480
Msp20I TGGCCA 1 cut(s) 2090
MspA1I CMGCKG 1 cut(s) 1376
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
MspR9I CCNGG 6 cut(s) 289, 294, 506, 518, 697, 1982
MunI CAATTG 1 cut(s) 2139
Mva1269I GAATGC 2 cut(s) 1290, 1945
MvaI CCWGG 2 cut(s) 697, 1982
MvnI CGCG 4 cut(s) 137, 278, 1326, 1689
NciI CCSGG 4 cut(s) 289, 294, 506, 518
NdeI CATATG 1 cut(s) 1050
NheI GCTAGC 1 cut(s) 1633
NlaIV GGNNCC 7 cut(s) 292, 504, 684, 799, 1070, 1071, 1782
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 1334, 1556
NruI TCGCGA 1 cut(s) 1689
NspI RCATGY 4 cut(s) 1240, 1871, 2399, 2491
NspV TTCGAA 1 cut(s) 2224
PaeI GCATGC 1 cut(s) 1871
PagI TCATGA 1 cut(s) 1926
PciI ACATGT 1 cut(s) 2395
PctI GAATGC 2 cut(s) 1290, 1945
PdmI GAANNNNTTC 1 cut(s) 2304
PfeI GAWTC 9 cut(s) 83, 139, 377, 511, 886, 892, 1214, 1805, 2002
PfoI TCCNGGA 1 cut(s) 516
Ple19I CGATCG 2 cut(s) 1342, 1664
PleI GAGTC 5 cut(s) 157, 208, 210, 439, 1444
PpsI GAGTC 5 cut(s) 157, 208, 210, 439, 1444
PpuMI RGGWCCY 3 cut(s) 502, 1069, 2095
PscI ACATGT 1 cut(s) 2395
PshBI ATTAAT 1 cut(s) 1314
PsiI TTATAA 1 cut(s) 852
Psp124BI GAGCTC 3 cut(s) 48, 840, 920
Psp1406I AACGTT 1 cut(s) 2167
Psp5II RGGWCCY 3 cut(s) 502, 1069, 2095
Psp6I CCWGG 2 cut(s) 695, 1980
PspFI CCCAGC 3 cut(s) 1037, 1247, 1637
PspGI CCWGG 2 cut(s) 695, 1980
PspN4I GGNNCC 7 cut(s) 292, 504, 684, 799, 1070, 1071, 1782
PspOMI GGGCCC 1 cut(s) 290
PspPI GGNCC 7 cut(s) 290, 291, 502, 1069, 1589, 2095, 2278
PspPPI RGGWCCY 3 cut(s) 502, 1069, 2095
PstI CTGCAG 2 cut(s) 1375, 1938
PstNI CAGNNNCTG 2 cut(s) 1358, 1379
PsuI RGATCY 2 cut(s) 31, 2323
PvuI CGATCG 2 cut(s) 1342, 1664
PvuII CAGCTG 1 cut(s) 1376
RruI TCGCGA 1 cut(s) 1689
RsaI GTAC 3 cut(s) 1571, 2027, 2129
RsaNI GTAC 3 cut(s) 1570, 2026, 2128
RseI CAYNNNNRTG 1 cut(s) 2480
SacI GAGCTC 3 cut(s) 48, 840, 920
Sau96I GGNCC 7 cut(s) 290, 291, 502, 1069, 1589, 2095, 2278
ScaI AGTACT 1 cut(s) 2027
SchI GAGTC 5 cut(s) 157, 209, 210, 440, 1445
ScrFI CCNGG 6 cut(s) 289, 294, 506, 518, 697, 1982
SduI GDGCHC 6 cut(s) 48, 294, 840, 920, 1118, 1518
SfaNI GCATC 7 cut(s) 1242, 1345, 1417, 1710, 1762, 1967, 2320
SfcI CTRYAG 2 cut(s) 1371, 1934
SfuI TTCGAA 1 cut(s) 2224
SinI GGWCC 3 cut(s) 502, 1069, 2095
SmiMI CAYNNNNRTG 1 cut(s) 2480
SmlI CTYRAG 4 cut(s) 225, 332, 1433, 2360
SmoI CTYRAG 4 cut(s) 225, 332, 1433, 2360
SpeI ACTAGT 1 cut(s) 1075
SphI GCATGC 1 cut(s) 1871
SsiI CCGC 7 cut(s) 186, 189, 317, 650, 863, 1096, 1534
SspI AATATT 2 cut(s) 1833, 2348
SspMI CTAG 5 cut(s) 270, 1076, 1364, 1634, 2327
SstI GAGCTC 3 cut(s) 48, 840, 920
StyD4I CCNGG 6 cut(s) 287, 292, 504, 516, 695, 1980
StyI CCWWGG 4 cut(s) 498, 897, 2091, 2158
TaaI ACNGT 5 cut(s) 644, 913, 1125, 1520, 1795
TaiI ACGT 2 cut(s) 2170, 2293
TaqI TCGA 7 cut(s) 352, 434, 639, 1310, 1446, 1855, 2224
TatI WGTACW 1 cut(s) 2025
TauI GCSGC 2 cut(s) 188, 191
TfiI GAWTC 9 cut(s) 83, 139, 377, 511, 886, 892, 1214, 1805, 2002
TscAI CASTG 4 cut(s) 918, 1128, 1523, 1918
TseFI GTSAC 2 cut(s) 1334, 1556
Tsp45I GTSAC 2 cut(s) 1334, 1556
TspGWI ACGGA 3 cut(s) 160, 1094, 1817
TspRI CASTG 4 cut(s) 918, 1128, 1523, 1918
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 3 cut(s) 502, 1069, 2095
VspI ATTAAT 1 cut(s) 1314
XapI RAATTY 7 cut(s) 364, 544, 635, 1181, 2219, 2300, 2438
XbaI TCTAGA 1 cut(s) 2326
XceI RCATGY 4 cut(s) 1240, 1871, 2399, 2491
XmnI GAANNNNTTC 1 cut(s) 2304
XspI CTAG 5 cut(s) 270, 1076, 1364, 1634, 2327
ZrmI AGTACT 1 cut(s) 2027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.