MD12G1055200.v1.1

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017505
Physical Location & Seq
Forward (+)
6239916 .. 6243440
3525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1055200.v1.1.491

Sequence Viewer

Length: 2754 bp
ATGATAATAATGCGGTTTTCTTTTTCTAAAACGAAAAAGAAAAACCCCATTTCCGCGCCCAACAAAAAAAATCAAAAACCGTGCAGTCACACTCAACACTCTGGCGGTCTCACCCTCACCAAATCACTCCCACACTCCCGCAAGATCCCCACTCTCCCTTCCTACTTCGACCTTCATCTCTCACTAATGGAGGAAGAAGACCAGCAAAAAGCTGCAGATCTGATCAAGGAGCTCGTCATCCGCCTCCGCTCCCAAAGTCCCAATTCCGAATCTCACCTGCTGACTCCAAACTCCCCGGAATTCCAGAGCTCGCTCCGCTACGCTTTCCGCTTGATCTCCAGCCGATTAACTCCGTCCGTGGCTCCCGACGCCGCCGCCATAGCCCAGTCCATCAAGCGCCGCCTCGCCACCCAAGGTAGGTCCTCGCAGGCCCTCACTTTCGCCGACCTTTATACCAAATTCGCGTCGAAAACCGGCCCGGGGAGTGTAAATAACAAATGGGCAGTCGTTTATTTGCTCAAAATTGTGTCGGAGGATCGGAAAAATGGGAAAACCCAGTTGGATTCTTCGGTTTTGTTGCCCAATTTGGGGTTAAATGATGCCGAATCGGGGAAGGAGTCAAGGGTTTTGGTGGGTAGGGAGAATAAGGAAAAGGGTTGGAACAATGGGGTATTGTTGGTTTCTAAAGACCCGGAAAATCTCCGCGAAATTGCTTTTAGGGAGTTTGTGAATTTGATTAAAGAAGAGAATGAGGTTTCTGAAGAGGTTTTGGTGAGAGATGTGTTGTATGCCTGTCAAGGAATTGATGGCAAGTACGTGAAATTTGATAGTAGTGCTGATGGTTATGCTTTATCGGATTTGATTAAGGTGCCTAGAGCAACCCGCATTATGGTTCGGAAGCTTTGTGAATTGGGGTGGTTGTTTAGGAAGGTTAAAGGGTATATTTCAGAGAGTATGGATGGGTTTCCATCTGACAATGTTGGAACCGTTGGGCAGGCCTTTTGTGCTGCGTTACAAGATGAGCTCTCCGATTACTATAAGTTGTTGGCTGTTCTTGAAGCGCAGTCAATGAATCCAATTCCTTTGGTTTCAGAGACTGCGAGCTCAGAGAATTATCTTTCTCTGAGGAGATTGTCTGTTTGGTTTGCGGAGCCAATGGTGAAAATGAGGTTAATGGCTGTTTTGGTTGATAAATGTAGGGTTTTAAGGGGTGGGGCAATGGCTGGGGCTATTCATTTACATGCCCAGCATGGTGACCCATTGGTGCACGAGTTCATGGGACGCTTACTTCGGAGGGTATGCTCTCCACTATTTGAGATGGTTAGGAGTTGGGTTTTGGAAGGGGAGTTAGAAGATGTTTTTGCAGAATTCTTTGTAGTGGGTCAGCCAGTGAAAGCTGAGTCTCTTTGGAGGGAAGGTTACATGCTCCATGCCGGAATGCTTCCTTCTTTTATTTCTCAATCGCTTGCTCAACGCATTCTGAGGACAGGCAAGTCAATCAATTTCCTTCGTGTCTGTTGTGAGGATCGTGGTTGGGCTGATGCTGCAACAGAAGCTGCAGCTGCTGCTGGTACCACGACAAGAAGATGGGGTCTTGGATATGGTGAAACTGATGCTCTTGAGTCTTTGGTTGATGGAGCAGCAAAGAGAGTTGATAAGCATTTGTTGGATGTTATTTACAACCAGTACAAGTTCAAGGAGCACTGTCTTGCTATAAAGCGGTATTTACTCCTCGGACAGGGTGACTTTGTTCAGTATCTAATGGATATAGTTGGGCCAGAGCTTTCTGAACCTGCTAACACAATAAGCTCTTTTCAGCTAGCTGGGTTGCTGGAAACTGCAATTCGAGCATCTAATGCTCAGTATGATGATCGTGACATATTGGATAGGTTGAAGGTAAAGATGATGCCACATGGAACCGGAGATAGGGGCTGGGATGTGTTCTCATTGGAATATGATGCAAGGGTTCCTTTAGTTACTGTTTTTACAGAATCTGTGATGACAAAGTATTTAAGAATTTTTAATTTCTTATGGAAGCTTCGGCGAGTAGAGCATGCCCTTATTGGTATTTGGAAGACAATGAAGCCAAATTGTATCACTTCACGTTCATTTATGAAGCTCCCGAATGCAGTTAAGTTGCAGTTGCTTTCAACTTTGAGACGATACCAGGTTCTTTGGGATGAGATGAATCATTTTGTCTCAAACTTGCAATACTATATTATGTTTGAAGTCTTGGAGGTGTCTTGGTCCAATTTTTTGAATGAAATGGATGTGGCTAAGGACCTCGATGATCTACTTGCCGCTCATGAAAAGTACCTCCATTCGATTTTGGAGAAATCCCTCCTTGGAGAACGTTCCCAAACCCTTTATGATTCACTCTTTGCCTTGTTTGATCTTATATTGAAATTCCGAAGTCATGCAGATCGATTGTCTGAAGGGATTAATGAGTTGCAAGCCAGAACCATGGAATCCTCTTTACCCTCTCGAGACAAGAGTAAAGCAAAAAAGCGATCAAATGATAGGTCGTCAGAGCCTGGATCCTGGATTAGTGAAGGCAGGAAAGCCCTCACGCAACGTGCTGGTGAATTTCTTCGGAATATGGGACAAGATTTAGATGCACTATCAAAAGAATATTCATCATTGCTTGAGGATTTTATTTCTAAGTTGCCCATGCAGCAACATGTTGATTTAAAGTTCCTCCTGTTTCGGCTGGACTTCACTGAGTTTTATAGCCAGTTGCATCCTAGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

918

Amino Acids

103.97

Weight (kDa)

8.59

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCP_N_terminal PF17681 258 - 561 4.6e-78 Gamma tubulin complex component N-terminal
GCP_C_terminal PF04130 567 - 910 7.6e-80 Gamma tubulin complex component C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06680
fragaria_vesca FvH4_6g21970
malus_domestica MD12G1055200.v1.1 MD14G1054400.v1.1
prunus_persica Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1
pyrus_communis pycom12g04820 pycom14g04490
rosa_laevigata RLG00000023588
rosa_roxburghii Rroxscaffold_6G00402450
rosa_samantha Rh3CG253200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 285
AasI GACNNNNNNGTC 1 cut(s) 1492
Acc36I ACCTGC 2 cut(s) 285, 1801
Acc65I GGTACC 1 cut(s) 1571
AccB1I GGYRCC 2 cut(s) 868, 1571
AccBSI CCGCTC 2 cut(s) 249, 2303
AccII CGCG 3 cut(s) 56, 464, 705
AclI AACGTT 1 cut(s) 2353
AclWI GGATC 5 cut(s) 139, 543, 1533, 2532, 2545
AcsI RAATTY 8 cut(s) 299, 458, 730, 821, 1367, 2016, 2405, 2585
AcuI CTGAAG 2 cut(s) 780, 2454
AcyI GRCGYC 1 cut(s) 369
AfaI GTAC 5 cut(s) 815, 1573, 1688, 2315, 2749
AfiI CCNNNNNNNGG 8 cut(s) 417, 480, 587, 588, 609, 889, 1738, 1926
AflIII ACRYGT 1 cut(s) 2680
AgsI TTSAA 7 cut(s) 1058, 1696, 1894, 2151, 2228, 2260, 2404
AjnI CCWGG 3 cut(s) 2166, 2533, 2540
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
Alw21I GWGCWC 6 cut(s) 234, 311, 1026, 1106, 1269, 1704
Alw26I GTCTC 6 cut(s) 113, 1088, 1407, 2152, 2203, 2481
Alw44I GTGCAC 1 cut(s) 1265
AlwI GGATC 5 cut(s) 139, 543, 1533, 2532, 2545
AlwNI CAGNNNCTG 5 cut(s) 1097, 1556, 1565, 1994, 2534
Ama87I CYCGRG 2 cut(s) 478, 2484
AoxI GGCC 4 cut(s) 429, 475, 996, 1775
ApaLI GTGCAC 1 cut(s) 1265
ApeKI GCWGC 9 cut(s) 212, 1007, 1544, 1556, 1559, 1562, 1565, 1640, 2674
ApoI RAATTY 8 cut(s) 299, 458, 730, 821, 1367, 2016, 2405, 2585
ArsI GACNNNNNNTTYG 6 cut(s) 489, 521, 1272, 1304, 2507, 2539
AseI ATTAAT 1 cut(s) 2442
Asp700I GAANNNNTTC 1 cut(s) 2589
Asp718I GGTACC 1 cut(s) 1571
AspLEI GCGC 3 cut(s) 58, 399, 1063
AspS9I GGNCC 6 cut(s) 420, 430, 476, 1775, 2247, 2281
AsuC2I CCSGG 4 cut(s) 296, 479, 480, 692
AsuHPI GGTGA 9 cut(s) 103, 109, 266, 784, 1171, 1265, 1616, 1754, 2594
AsuNHI GCTAGC 1 cut(s) 1819
AvaI CYCGRG 2 cut(s) 478, 2484
AvaII GGWCC 3 cut(s) 420, 2247, 2281
BaeGI GKGCMC 1 cut(s) 1269
BamHI GGATCC 1 cut(s) 2537
BanI GGYRCC 2 cut(s) 868, 1571
BanII GRGCYC 4 cut(s) 234, 311, 1026, 1106
BauI CACGAG 1 cut(s) 1268
BbsI GAAGAC 2 cut(s) 204, 2081
Bbv12I GWGCWC 6 cut(s) 234, 311, 1026, 1106, 1269, 1704
BbvI GCAGC 9 cut(s) 199, 994, 1531, 1543, 1549, 1552, 1571, 1652, 2686
BccI CCATC 8 cut(s) 398, 800, 833, 953, 976, 1312, 1581, 1628
BciT130I CCWGG 3 cut(s) 2168, 2535, 2542
BclI TGATCA 1 cut(s) 222
BcnI CCSGG 4 cut(s) 296, 479, 480, 692
BcoDI GTCTC 6 cut(s) 113, 1088, 1407, 2152, 2203, 2481
BfaI CTAG 3 cut(s) 873, 1820, 2745
BfmI CTRYAG 2 cut(s) 213, 1557
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 2 cut(s) 285, 1801
BglII AGATCT 1 cut(s) 217
Bme1390I CCNGG 7 cut(s) 296, 479, 480, 692, 2168, 2535, 2542
Bme18I GGWCC 3 cut(s) 420, 2247, 2281
BmeT110I CYCGRG 2 cut(s) 478, 2484
BmgT120I GGNCC 6 cut(s) 420, 430, 476, 1775, 2247, 2281
BmiI GGNNCC 8 cut(s) 363, 870, 985, 1152, 1573, 1918, 1968, 2539
BmrFI CCNGG 7 cut(s) 296, 479, 480, 692, 2168, 2535, 2542
BmrI ACTGGG 2 cut(s) 379, 550
BmsI GCATC 8 cut(s) 589, 1531, 1603, 1859, 1896, 1948, 2605, 2749
BmtI GCTAGC 1 cut(s) 1823
BmuI ACTGGG 2 cut(s) 379, 550
BpiI GAAGAC 2 cut(s) 204, 2081
BpmI CTGGAG 1 cut(s) 322
Bpu10I CCTNAGC 1 cut(s) 2277
BpuEI CTTGAG 2 cut(s) 1640, 2666
BpuMI CCSGG 4 cut(s) 296, 479, 480, 692
Bsa29I ATCGAT 1 cut(s) 2425
BsaAI YACGTR 2 cut(s) 817, 2751
BsaHI GRCGYC 1 cut(s) 369
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 8 cut(s) 294, 357, 412, 478, 479, 1732, 2344, 2463
BsaWI WCCGGW 1 cut(s) 1919
BsaXI ACNNNNNCTCC 2 cut(s) 1402, 1432
Bsc4I CCNNNNNNNGG 8 cut(s) 417, 480, 587, 588, 609, 889, 1738, 1926
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 5 cut(s) 385, 556, 1388, 1684, 2734
Bse3DI GCAATG 2 cut(s) 1224, 2639
BseBI CCWGG 3 cut(s) 2168, 2535, 2542
BseCI ATCGAT 1 cut(s) 2425
BseDI CCNNGG 8 cut(s) 294, 357, 412, 478, 479, 1732, 2344, 2463
BseGI GGATG 7 cut(s) 237, 964, 1675, 1942, 2185, 2275, 2740
BseLI CCNNNNNNNGG 8 cut(s) 417, 480, 587, 588, 609, 889, 1738, 1926
BseMI GCAATG 2 cut(s) 1224, 2639
BseMII CTCAG 6 cut(s) 1115, 1119, 1389, 1472, 1874, 2712
BseNI ACTGG 5 cut(s) 385, 556, 1388, 1684, 2734
BseRI GAGGAG 2 cut(s) 1141, 1721
BseSI GKGCMC 1 cut(s) 1269
BseXI GCAGC 9 cut(s) 199, 994, 1531, 1543, 1549, 1552, 1571, 1652, 2686
BseYI CCCAGC 4 cut(s) 1223, 1245, 1823, 1932
BsgI GTGCAG 1 cut(s) 103
Bsh1236I CGCG 3 cut(s) 56, 464, 705
BshFI GGCC 4 cut(s) 431, 477, 998, 1777
BshNI GGYRCC 2 cut(s) 868, 1571
BshVI ATCGAT 1 cut(s) 2425
BsiHKAI GWGCWC 6 cut(s) 234, 311, 1026, 1106, 1269, 1704
BsiHKCI CYCGRG 2 cut(s) 478, 2484
BsiSI CCGG 6 cut(s) 296, 474, 479, 692, 1434, 1920
BslFI GGGAC 3 cut(s) 243, 1293, 2616
BslI CCNNNNNNNGG 8 cut(s) 417, 480, 587, 588, 609, 889, 1738, 1926
BsmAI GTCTC 6 cut(s) 113, 1088, 1407, 2152, 2203, 2481
BsmBI CGTCTC 1 cut(s) 2152
BsmFI GGGAC 3 cut(s) 243, 1293, 2616
BsmI GAATGC 3 cut(s) 1443, 1476, 2131
BsnI GGCC 4 cut(s) 431, 477, 998, 1777
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 2 cut(s) 478, 2484
Bsp1286I GDGCHC 6 cut(s) 234, 311, 1026, 1106, 1269, 1704
Bsp19I CCATGG 1 cut(s) 2463
BspANI GGCC 4 cut(s) 431, 477, 998, 1777
BspCNI CTCAG 6 cut(s) 1116, 1118, 1390, 1473, 1873, 2713
BspDI ATCGAT 1 cut(s) 2425
BspFNI CGCG 3 cut(s) 56, 464, 705
BspHI TCATGA 1 cut(s) 2305
BspLI GGNNCC 8 cut(s) 363, 870, 985, 1152, 1573, 1918, 1968, 2539
BspMAI CTGCAG 2 cut(s) 217, 1561
BspMI ACCTGC 2 cut(s) 285, 1801
BspOI GCTAGC 1 cut(s) 1823
BspPI GGATC 5 cut(s) 139, 543, 1533, 2532, 2545
BspT107I GGYRCC 2 cut(s) 868, 1571
BspTNI GGTCTC 1 cut(s) 113
BsrBI CCGCTC 2 cut(s) 249, 2303
BsrDI GCAATG 2 cut(s) 1224, 2639
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 5 cut(s) 385, 556, 1388, 1684, 2734
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 8 cut(s) 294, 357, 412, 478, 479, 1732, 2344, 2463
BssNI GRCGYC 1 cut(s) 369
BssSI CACGAG 1 cut(s) 1268
BssT1I CCWWGG 3 cut(s) 412, 2344, 2463
Bst2BI CACGAG 1 cut(s) 1268
Bst2UI CCWGG 3 cut(s) 2168, 2535, 2542
Bst4CI ACNGT 4 cut(s) 81, 988, 1706, 1981
Bst6I CTCTTC 2 cut(s) 738, 756
BstACI GRCGYC 1 cut(s) 369
BstAPI GCANNNNNTGC 2 cut(s) 1565, 1856
BstBAI YACGTR 2 cut(s) 817, 2751
BstC8I GCNNGC 8 cut(s) 311, 429, 996, 1102, 1467, 1821, 2055, 2454
BstDEI CTNAG 8 cut(s) 1105, 1124, 1398, 1481, 1860, 2277, 2661, 2721
BstDSI CCRYGG 2 cut(s) 357, 2463
BstEII GGTNACC 1 cut(s) 1253
BstENI CCTNNNNNAGG 1 cut(s) 1736
BstF5I GGATG 7 cut(s) 237, 964, 1675, 1942, 2185, 2275, 2740
BstFNI CGCG 3 cut(s) 56, 464, 705
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 3 cut(s) 58, 399, 1063
BstMAI GTCTC 6 cut(s) 113, 1088, 1407, 2152, 2203, 2481
BstNI CCWGG 3 cut(s) 2168, 2535, 2542
BstNSI RCATGY 4 cut(s) 1244, 1426, 2057, 2684
BstPI GGTNACC 1 cut(s) 1253
BstSCI CCNGG 7 cut(s) 294, 477, 478, 690, 2166, 2533, 2540
BstSFI CTRYAG 2 cut(s) 213, 1557
BstSLI GKGCMC 1 cut(s) 1269
BstSNI TACGTA 1 cut(s) 2751
BstUI CGCG 3 cut(s) 56, 464, 705
BstV1I GCAGC 9 cut(s) 199, 994, 1531, 1543, 1549, 1552, 1571, 1652, 2686
BstV2I GAAGAC 2 cut(s) 204, 2081
BstX2I RGATCY 3 cut(s) 144, 217, 2537
BstXI CCANNNNNNTGG 1 cut(s) 2464
BstYI RGATCY 3 cut(s) 144, 217, 2537
Bsu15I ATCGAT 1 cut(s) 2425
BsuRI GGCC 4 cut(s) 431, 477, 998, 1777
BsuTUI ATCGAT 1 cut(s) 2425
BtgI CCRYGG 2 cut(s) 357, 2463
BtsCI GGATG 7 cut(s) 237, 964, 1675, 1942, 2185, 2275, 2740
BtsIMutI CAGTG 3 cut(s) 1395, 1702, 2718
BveI ACCTGC 2 cut(s) 285, 1801
Cac8I GCNNGC 8 cut(s) 311, 429, 996, 1102, 1467, 1821, 2055, 2454
CaiI CAGNNNCTG 5 cut(s) 1097, 1556, 1565, 1994, 2534
CciI TCATGA 1 cut(s) 2305
CfoI GCGC 3 cut(s) 58, 399, 1063
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 6 cut(s) 420, 430, 476, 1775, 2247, 2281
Cfr9I CCCGGG 1 cut(s) 478
ClaI ATCGAT 1 cut(s) 2425
CseI GACGC 3 cut(s) 377, 453, 1290
CsiI ACCWGGT 1 cut(s) 2166
Csp6I GTAC 5 cut(s) 814, 1572, 1687, 2314, 2748
CviQI GTAC 5 cut(s) 814, 1572, 1687, 2314, 2748
DdeI CTNAG 8 cut(s) 1105, 1124, 1398, 1481, 1860, 2277, 2661, 2721
DraI TTTAAA 1 cut(s) 2691
DrdI GACNNNNNNGTC 1 cut(s) 1492
DseDI GACNNNNNNGTC 1 cut(s) 1492
Eam1104I CTCTTC 2 cut(s) 738, 756
EarI CTCTTC 2 cut(s) 738, 756
EciI GGCGGA 1 cut(s) 230
Ecl136II GAGCTC 4 cut(s) 232, 309, 1024, 1104
Eco105I TACGTA 1 cut(s) 2751
Eco130I CCWWGG 3 cut(s) 412, 2344, 2463
Eco147I AGGCCT 1 cut(s) 998
Eco24I GRGCYC 4 cut(s) 234, 311, 1026, 1106
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 3 cut(s) 420, 2247, 2281
Eco53kI GAGCTC 4 cut(s) 232, 309, 1024, 1104
Eco57I CTGAAG 2 cut(s) 780, 2454
Eco88I CYCGRG 2 cut(s) 478, 2484
Eco91I GGTNACC 1 cut(s) 1253
EcoICRI GAGCTC 4 cut(s) 232, 309, 1024, 1104
EcoNI CCTNNNNNAGG 1 cut(s) 1736
EcoO109I RGGNCCY 3 cut(s) 420, 430, 2281
EcoO65I GGTNACC 1 cut(s) 1253
EcoRI GAATTC 2 cut(s) 299, 1367
EcoRII CCWGG 3 cut(s) 2166, 2533, 2540
EcoT14I CCWWGG 3 cut(s) 412, 2344, 2463
EcoT38I GRGCYC 4 cut(s) 234, 311, 1026, 1106
ErhI CCWWGG 3 cut(s) 412, 2344, 2463
Esp3I CGTCTC 1 cut(s) 2152
FalI AAGNNNNNCTT 2 cut(s) 1954, 1986
FaqI GGGAC 3 cut(s) 243, 1293, 2616
FauI CCCGC 2 cut(s) 146, 890
FbaI TGATCA 1 cut(s) 222
FokI GGATG 7 cut(s) 224, 971, 1682, 1949, 2192, 2282, 2727
FriOI GRGCYC 4 cut(s) 234, 311, 1026, 1106
FspBI CTAG 3 cut(s) 873, 1820, 2745
GlaI GCGC 3 cut(s) 57, 398, 1062
GsaI CCCAGC 4 cut(s) 1227, 1249, 1827, 1936
GsuI CTGGAG 1 cut(s) 322
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 4 cut(s) 431, 477, 998, 1777
HapII CCGG 6 cut(s) 296, 474, 479, 692, 1434, 1920
HgaI GACGC 3 cut(s) 377, 453, 1290
HhaI GCGC 3 cut(s) 58, 399, 1063
Hin1I GRCGYC 1 cut(s) 369
Hin6I GCGC 3 cut(s) 56, 397, 1061
HinP1I GCGC 3 cut(s) 56, 397, 1061
HindIII AAGCTT 2 cut(s) 899, 2036
HpaII CCGG 6 cut(s) 296, 474, 479, 692, 1434, 1920
HphI GGTGA 9 cut(s) 103, 109, 266, 784, 1171, 1265, 1616, 1754, 2594
Hpy166II GTNNAC 1 cut(s) 1267
Hpy188III TCNNGA 9 cut(s) 304, 365, 1055, 1619, 1874, 2122, 2306, 2484, 2486
Hpy8I GTNNAC 1 cut(s) 1267
Hpy99I CGWCG 2 cut(s) 371, 469
HpyCH4III ACNGT 4 cut(s) 81, 988, 1706, 1981
HpyCH4IV ACGT 5 cut(s) 816, 2104, 2353, 2575, 2750
HpyF3I CTNAG 8 cut(s) 1105, 1124, 1398, 1481, 1860, 2277, 2661, 2721
HpySE526I ACGT 5 cut(s) 816, 2104, 2353, 2575, 2750
Hsp92I GRCGYC 1 cut(s) 369
HspAI GCGC 3 cut(s) 56, 397, 1061
KpnI GGTACC 1 cut(s) 1575
Ksp22I TGATCA 1 cut(s) 222
LmnI GCTCC 9 cut(s) 229, 254, 318, 367, 1150, 1431, 1637, 1699, 2124
Lsp1109I GCAGC 9 cut(s) 199, 994, 1531, 1543, 1549, 1552, 1571, 1652, 2686
LweI GCATC 8 cut(s) 589, 1531, 1603, 1859, 1896, 1948, 2605, 2749
MabI ACCWGGT 1 cut(s) 2166
MaeI CTAG 3 cut(s) 873, 1820, 2745
MaeII ACGT 5 cut(s) 816, 2104, 2353, 2575, 2750
MaeIII GTNAC 7 cut(s) 86, 1011, 1253, 1418, 1742, 1874, 1975
MbiI CCGCTC 2 cut(s) 249, 2303
MboII GAAGA 9 cut(s) 206, 209, 558, 755, 773, 1364, 1596, 2086, 2582
MflI RGATCY 3 cut(s) 144, 217, 2537
MhlI GDGCHC 6 cut(s) 234, 311, 1026, 1106, 1269, 1704
MlyI GAGTC 4 cut(s) 277, 626, 1409, 1631
MmeI TCCRAC 5 cut(s) 510, 540, 638, 961, 1647
MroXI GAANNNNTTC 1 cut(s) 2589
MslI CAYNNNNRTG 1 cut(s) 1239
MspA1I CMGCKG 1 cut(s) 1562
MspI CCGG 6 cut(s) 296, 474, 479, 692, 1434, 1920
MspR9I CCNGG 7 cut(s) 296, 479, 480, 692, 2168, 2535, 2542
Mva1269I GAATGC 3 cut(s) 1443, 1476, 2131
MvaI CCWGG 3 cut(s) 2168, 2535, 2542
MvnI CGCG 3 cut(s) 56, 464, 705
NciI CCSGG 4 cut(s) 296, 479, 480, 692
NcoI CCATGG 1 cut(s) 2463
NheI GCTAGC 1 cut(s) 1819
NlaIV GGNNCC 8 cut(s) 363, 870, 985, 1152, 1573, 1918, 1968, 2539
NmuCI GTSAC 4 cut(s) 86, 1253, 1742, 1874
NspI RCATGY 4 cut(s) 1244, 1426, 2057, 2684
PaeI GCATGC 1 cut(s) 2057
PaeR7I CTCGAG 1 cut(s) 2484
PagI TCATGA 1 cut(s) 2305
PaqCI CACCTGC 1 cut(s) 285
PceI AGGCCT 1 cut(s) 998
PciI ACATGT 1 cut(s) 2680
PctI GAATGC 3 cut(s) 1443, 1476, 2131
PdmI GAANNNNTTC 1 cut(s) 2589
PfeI GAWTC 8 cut(s) 269, 563, 605, 1072, 1991, 2188, 2372, 2468
PfoI TCCNGGA 1 cut(s) 2540
PleI GAGTC 4 cut(s) 277, 625, 1408, 1630
PpsI GAGTC 4 cut(s) 277, 625, 1408, 1630
Ppu21I YACGTR 2 cut(s) 817, 2751
PpuMI RGGWCCY 2 cut(s) 420, 2281
PscI ACATGT 1 cut(s) 2680
PshBI ATTAAT 1 cut(s) 2442
Psp124BI GAGCTC 4 cut(s) 234, 311, 1026, 1106
Psp1406I AACGTT 1 cut(s) 2353
Psp5II RGGWCCY 2 cut(s) 420, 2281
Psp6I CCWGG 3 cut(s) 2166, 2533, 2540
PspEI GGTNACC 1 cut(s) 1253
PspFI CCCAGC 4 cut(s) 1223, 1245, 1823, 1932
PspGI CCWGG 3 cut(s) 2166, 2533, 2540
PspN4I GGNNCC 8 cut(s) 363, 870, 985, 1152, 1573, 1918, 1968, 2539
PspPI GGNCC 6 cut(s) 420, 430, 476, 1775, 2247, 2281
PspPPI RGGWCCY 2 cut(s) 420, 2281
PstI CTGCAG 2 cut(s) 217, 1561
PstNI CAGNNNCTG 5 cut(s) 1097, 1556, 1565, 1994, 2534
PsuI RGATCY 3 cut(s) 144, 217, 2537
PvuII CAGCTG 1 cut(s) 1562
RsaI GTAC 5 cut(s) 815, 1573, 1688, 2315, 2749
RsaNI GTAC 5 cut(s) 814, 1572, 1687, 2314, 2748
RseI CAYNNNNRTG 1 cut(s) 1239
SacI GAGCTC 4 cut(s) 234, 311, 1026, 1106
Sau96I GGNCC 6 cut(s) 420, 430, 476, 1775, 2247, 2281
SchI GAGTC 4 cut(s) 277, 626, 1409, 1631
ScrFI CCNGG 7 cut(s) 296, 479, 480, 692, 2168, 2535, 2542
SduI GDGCHC 6 cut(s) 234, 311, 1026, 1106, 1269, 1704
SexAI ACCWGGT 1 cut(s) 2166
SfaNI GCATC 8 cut(s) 589, 1531, 1603, 1859, 1896, 1948, 2605, 2749
SfcI CTRYAG 2 cut(s) 213, 1557
Sfr274I CTCGAG 1 cut(s) 2484
SinI GGWCC 3 cut(s) 420, 2247, 2281
SlaI CTCGAG 1 cut(s) 2484
SmaI CCCGGG 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 1239
SmlI CTYRAG 3 cut(s) 1619, 2484, 2645
SmoI CTYRAG 3 cut(s) 1619, 2484, 2645
SnaBI TACGTA 1 cut(s) 2751
SphI GCATGC 1 cut(s) 2057
SseBI AGGCCT 1 cut(s) 998
SspI AATATT 1 cut(s) 2633
SspMI CTAG 3 cut(s) 873, 1820, 2745
SstI GAGCTC 4 cut(s) 234, 311, 1026, 1106
StuI AGGCCT 1 cut(s) 998
StyD4I CCNGG 7 cut(s) 294, 477, 478, 690, 2166, 2533, 2540
StyI CCWWGG 3 cut(s) 412, 2344, 2463
TaaI ACNGT 4 cut(s) 81, 988, 1706, 1981
TaiI ACGT 5 cut(s) 819, 2107, 2356, 2578, 2753
TaqI TCGA 7 cut(s) 168, 467, 1846, 2286, 2324, 2425, 2485
TatI WGTACW 1 cut(s) 1686
TauI GCSGC 4 cut(s) 374, 377, 402, 2303
TfiI GAWTC 8 cut(s) 269, 563, 605, 1072, 1991, 2188, 2372, 2468
TscAI CASTG 3 cut(s) 1395, 1709, 2725
TseFI GTSAC 4 cut(s) 86, 1253, 1742, 1874
TseI GCWGC 9 cut(s) 212, 1007, 1544, 1556, 1559, 1562, 1565, 1640, 2674
Tsp45I GTSAC 4 cut(s) 86, 1253, 1742, 1874
TspGWI ACGGA 2 cut(s) 342, 346
TspMI CCCGGG 1 cut(s) 478
TspRI CASTG 3 cut(s) 1395, 1709, 2725
VneI GTGCAC 1 cut(s) 1265
VpaK11BI GGWCC 3 cut(s) 420, 2247, 2281
VspI ATTAAT 1 cut(s) 2442
XagI CCTNNNNNAGG 1 cut(s) 1736
XapI RAATTY 8 cut(s) 299, 458, 730, 821, 1367, 2016, 2405, 2585
XceI RCATGY 4 cut(s) 1244, 1426, 2057, 2684
XhoI CTCGAG 1 cut(s) 2484
XmaI CCCGGG 1 cut(s) 478
XmnI GAANNNNTTC 1 cut(s) 2589
XspI CTAG 3 cut(s) 873, 1820, 2745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.