RLG00000023588

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017505
Physical Location & Seq
Reverse (-)
25656041 .. 25659022
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023588

Sequence Viewer

Length: 2649 bp
ATGGAAGAAGAAGACCAGCAAAAAGTCGCAGATCTGGTCAAGGAGCTCGTCATTCGCTTGGTCGCCCAAAACTCCACATCCGAATCTCACCCACCAAATCCCAACTCCCCTCATCTCCAAACCTCACTTCGCTACGCGATTCGCTTACTCTCCAGCCGATTGACTCCCTCCGTCGCCCCCGACGCCGCCGCCATCGCCGAGTCCACCAAACGCCGACTCGCCACTCAAGGTAAGTCCTCGCAAGCCCTAACTTTCGCCGACCTTTATACTAAGTTCGCATCCAAAACCGGGCCCGGAAGTGTAAATAACAAGTGGGCGGTTCTCTATTTGCTTAAGATTATATCGGAGGATCGAAAGAGTGTGAATTCCCAGTTGGATTCTTCGGTTTTGTTGCCCAATTTGGGTTTAAACGACGCCGGATCGGGCGACGAGTCGAGGGTTTTGGGGGGAAGGGGAAGTGGGGAAAAGGGCTGGAGCAATGGGGTCTTGTTAGTTTCCAAGGACCCGGAGAATCTCCGGGAAATTGCGTTTATGGAGTTTGTGAATTTGGTTAAGGAAGAGAATGAAGTTTCTGAAGAGGCTTTGGTGAGAGATGTGTTGTATGCTTGTCAGGGAATTGATGGGAAGTATGTGAAATTCGACAGTAATGCGGATGGCTATGTTTTGTCTGATTTGATAAAGGTTCCGAGAGCTACCAGGATTATGGTTCGGAAGCTTTGTGAATTGGGGTGGTTGTTTAGGAAGGTTAAGGGGTATATCTCAGAGAGTATGGATGGGTTTCCATCCGAAGATGTTGGAACCATTGGGCAGGCTTTTTGTGCTGCATTGCAAGATGAGCTCTCCGATTATTATAAGTTGTTGGCGGTGCTTGAAGCACAGGCGATGAATCCGATTCCCTTGGTTTCAGAGACAGTGAGCTCGGGGAATTATCTTTCTATGAGGAGGTTGTCTGTTTGGTTTGCAGAGCCAATGGTGAAAATGAGGTTGATGGCTGTTTTGGTTGATAAATGTAGGGTTTTAAGGGGTGGGGCAATGGCTGGGGCTATTCATATGCATGCTCAGCATGGAGACCCACTAGTGCATGAGTTCATGGGGCGGTTACTCCGTCGGGTGTGCTCCCCACTGTTTGAGATGGTCAGGAGTTGGGTTTTGGAAGGGGAGTTAGAAGATGTTTTTGCTGAATTCTTTATCGTGGGTCAGCCGGTGAAAGCTGAATCTCTTTGGAGGGAAGGTTACATGCTCCATGCTGGGATGCTTCCTTCTTTTATTTCACAATCTCTTGCTCAACGCATTCTGAGGACTGGAAAGTCGATTAATTTCCTTCGCGTCTGTTGTGACGATCGTGGTTGGGCAGATGCTGCACTAGAAGCTGCAGCTGCTGCTGGCACCACGACAAGAAGATGGGGTCTTGGATATGGTGAAACTGATGCTCTTGAGTCTTTGGTCGATGGAGCAGCAAAGAGAGTTGATAAGCATTTGTTGGATGTTATATACAACCACTATAAGTTCAAGGAGCACTGTCTTGCAATAAAGCGGTATTTACTGCTTGGACAGGGTGACTTTGTTCAGTACCTAATGGATATTGTTGGGCCAGAGCTTTCTGAGCCTGCTAACACTATAAGCTCTTTCCAGCTAGCTGGGTTGCTGGAAACTGCAATCCGATCGTCTAATGCACAATATGATGATCGTGACATATTGGATAGATTGAAGGTAAAGATGATGCCACATGAAACTGGAGATAGAGGTTGGGATGTATTCTCATTGGAATATGATGCAAGGGTTCCTTTAGATACTGTTTTTACAGAATCCGTGATGACAAAGTATTTAAGAATATTTAATTTCTTGTGGAAGCTTCGACGAGTAGAGCATGCTCTTATTGGTGCCTGGAAGACTATGAAACCAAATTGTATCACTGCCCGTTCCTTCATGAAGCTGCAGAATGCAGTTAAGTTGCCGTTGCTTTCAACTCTGAGACGATGCCAGGTTCTTTGGGATGAGATGAATCATTTTGTTTCAAACTTGCAGTACTATATTATGTTTGAAGTCTTGGAGGTGTCATGGTCTGACTTTTTGAACGAAATGGAGGTGGCCAAGGACCTTGATGATCTACTTGCTGCACATGAAAAGTACCTCCATTCGATTGTGGAGAAATCCCTCCTTGGAGAACGTTCCCAAACCCTTTACAGCTCACTCTTTGCCTTGTTTGATCTTATATTGAAATTTCGAAGTCATGCTGATAGATTGTCTGAAGGGATCAATGAGTTGCAAGCAAGAACCATGGAATCCTCTGCACCCTCTCGAGTCAAGAGTAAAACAAAAAAGCGATTAAATGATAGATCCTCAGAGCCTGGGTCCTGGATTAGTGATGGCAGGAAGGCCCTCACACAACGTGCTGGTGAATTTCTTCGGAATATGGGACAGGATCTAGATGCAGTAGCAAAAGAATATTCGTCATTGCTTGAGGATTTTATTTCTATGTTGCCCATGCAGCAACATGTTGATTTAAAGTTCCTCCTGTTTCGGCTTGACTTCACCGAATTTTACAGCCAGTTGCGTCCTAGTACGACCAACCGATTTACCTTATATTGCTTGAGTGTTAGATGGTGTTTGCTTCATTCTGATGGGCATGTGTGGAAGTTTATGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

883

Amino Acids

99.75

Weight (kDa)

6.39

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCP_N_terminal PF17681 196 - 499 1.5e-77 Gamma tubulin complex component N-terminal
GCP_C_terminal PF04130 505 - 848 3.1e-81 Gamma tubulin complex component C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06680
fragaria_vesca FvH4_6g21970
malus_domestica MD12G1055200.v1.1 MD14G1054400.v1.1
prunus_persica Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1
pyrus_communis pycom12g04820 pycom14g04490
rosa_laevigata RLG00000023588
rosa_roxburghii Rroxscaffold_6G00402450
rosa_samantha Rh3CG253200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
AasI GACNNNNNNGTC 1 cut(s) 1306
AccB1I GGYRCC 2 cut(s) 1385, 1880
AccII CGCG 2 cut(s) 137, 1326
AciI CCGC 7 cut(s) 186, 189, 317, 650, 863, 1096, 1534
AclI AACGTT 1 cut(s) 2167
AclWI GGATC 5 cut(s) 357, 427, 2261, 2331, 2430
AcoI YGGCCR 1 cut(s) 2088
AcsI RAATTY 7 cut(s) 364, 544, 635, 1181, 2219, 2399, 2537
AcuI CTGAAG 2 cut(s) 594, 2268
AcyI GRCGYC 2 cut(s) 183, 414
AdeI CACNNNGTG 1 cut(s) 2390
AfaI GTAC 4 cut(s) 1571, 2027, 2129, 2563
AfiI CCNNNNNNNGG 2 cut(s) 288, 401
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 2494
AgsI TTSAA 8 cut(s) 872, 1510, 1708, 1965, 2016, 2042, 2074, 2218
AhlI ACTAGT 1 cut(s) 1075
AjnI CCWGG 5 cut(s) 695, 1883, 1980, 2347, 2354
AjuI GAANNNNNNNTTGG 4 cut(s) 111, 143, 356, 388
Alw21I GWGCWC 5 cut(s) 48, 840, 920, 1118, 1518
Alw26I GTCTC 3 cut(s) 902, 1062, 1966
AlwI GGATC 5 cut(s) 357, 427, 2261, 2331, 2430
AlwNI CAGNNNCTG 3 cut(s) 1358, 1379, 2348
Ama87I CYCGRG 2 cut(s) 919, 2298
AoxI GGCC 4 cut(s) 290, 1589, 2088, 2376
ApaI GGGCCC 1 cut(s) 294
ApoI RAATTY 7 cut(s) 364, 544, 635, 1181, 2219, 2399, 2537
AseI ATTAAT 1 cut(s) 1314
Asp700I GAANNNNTTC 1 cut(s) 2403
AspS9I GGNCC 7 cut(s) 290, 291, 502, 1589, 2095, 2352, 2377
AsuC2I CCSGG 4 cut(s) 289, 294, 506, 518
AsuHPI GGTGA 8 cut(s) 80, 598, 985, 1216, 1430, 1568, 2408, 2524
AsuII TTCGAA 1 cut(s) 2224
AsuNHI GCTAGC 1 cut(s) 1633
AvaI CYCGRG 2 cut(s) 919, 2298
AvaII GGWCC 3 cut(s) 502, 2095, 2352
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 2090
BanI GGYRCC 2 cut(s) 1385, 1880
BanII GRGCYC 4 cut(s) 48, 294, 840, 920
BbsI GAAGAC 2 cut(s) 18, 1895
Bbv12I GWGCWC 5 cut(s) 48, 840, 920, 1118, 1518
BceAI ACGGC 1 cut(s) 1939
BcgI CGANNNNNNTGC 4 cut(s) 629, 663, 1644, 1678
BciT130I CCWGG 5 cut(s) 697, 1885, 1982, 2349, 2356
BcnI CCSGG 4 cut(s) 289, 294, 506, 518
BcoDI GTCTC 3 cut(s) 902, 1062, 1966
BcuI ACTAGT 1 cut(s) 1075
BfaI CTAG 5 cut(s) 1076, 1364, 1634, 2426, 2559
BfmI CTRYAG 2 cut(s) 1371, 1934
BfrI CTTAAG 1 cut(s) 332
BglII AGATCT 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 1059
BmcAI AGTACT 1 cut(s) 2027
Bme1390I CCNGG 9 cut(s) 289, 294, 506, 518, 697, 1885, 1982, 2349, 2356
Bme18I GGWCC 3 cut(s) 502, 2095, 2352
BmeT110I CYCGRG 2 cut(s) 919, 2298
BmgT120I GGNCC 7 cut(s) 290, 291, 502, 1589, 2095, 2352, 2377
BmiI GGNNCC 8 cut(s) 292, 504, 684, 799, 1387, 1782, 1882, 2353
BmrFI CCNGG 9 cut(s) 289, 294, 506, 518, 697, 1885, 1982, 2349, 2356
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 8 cut(s) 287, 1242, 1345, 1417, 1710, 1762, 1967, 2419
BmtI GCTAGC 1 cut(s) 1637
BmuI ACTGGG 1 cut(s) 364
BpiI GAAGAC 2 cut(s) 18, 1895
BpmI CTGGAG 3 cut(s) 136, 493, 1755
Bpu1102I GCTNAGC 1 cut(s) 1059
Bpu14I TTCGAA 1 cut(s) 2224
BpuEI CTTGAG 4 cut(s) 210, 1454, 2480, 2611
BpuMI CCSGG 4 cut(s) 289, 294, 506, 518
BsaHI GRCGYC 2 cut(s) 183, 414
BsaI GGTCTC 1 cut(s) 1062
BsaJI CCNNGG 6 cut(s) 498, 897, 2091, 2158, 2277, 2348
BsaXI ACNNNNNCTCC 2 cut(s) 1216, 1246
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 401
Bse118I RCCGGY 1 cut(s) 1201
Bse1I ACTGG 4 cut(s) 370, 1306, 1738, 2548
Bse3DI GCAATG 4 cut(s) 484, 824, 1038, 2453
BseBI CCWGG 5 cut(s) 697, 1885, 1982, 2349, 2356
BseDI CCNNGG 6 cut(s) 498, 897, 2091, 2158, 2277, 2348
BseGI GGATG 9 cut(s) 77, 278, 658, 778, 782, 1257, 1489, 1756, 1999
BseLI CCNNNNNNNGG 2 cut(s) 288, 401
BseMI GCAATG 4 cut(s) 484, 824, 1038, 2453
BseMII CTCAG 6 cut(s) 774, 1073, 1286, 1593, 1961, 2355
BseNI ACTGG 4 cut(s) 370, 1306, 1738, 2548
BseRI GAGGAG 1 cut(s) 955
BseSI GKGCMC 1 cut(s) 294
BseYI CCCAGC 3 cut(s) 1037, 1247, 1637
BsgI GTGCAG 3 cut(s) 1344, 2100, 2274
Bsh1236I CGCG 2 cut(s) 137, 1326
Bsh1285I CGRYCG 2 cut(s) 1342, 1664
BshFI GGCC 4 cut(s) 292, 1591, 2090, 2378
BshNI GGYRCC 2 cut(s) 1385, 1880
BsiEI CGRYCG 2 cut(s) 1342, 1664
BsiHKAI GWGCWC 5 cut(s) 48, 840, 920, 1118, 1518
BsiHKCI CYCGRG 2 cut(s) 919, 2298
BsiSI CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
BslFI GGGAC 1 cut(s) 2430
BslI CCNNNNNNNGG 2 cut(s) 288, 401
BsmAI GTCTC 3 cut(s) 902, 1062, 1966
BsmBI CGTCTC 1 cut(s) 1966
BsmFI GGGAC 1 cut(s) 2430
BsmI GAATGC 2 cut(s) 1290, 1945
BsnI GGCC 4 cut(s) 292, 1591, 2090, 2378
Bso31I GGTCTC 1 cut(s) 1062
BsoBI CYCGRG 2 cut(s) 919, 2298
Bsp119I TTCGAA 1 cut(s) 2224
Bsp120I GGGCCC 1 cut(s) 290
Bsp1286I GDGCHC 6 cut(s) 48, 294, 840, 920, 1118, 1518
Bsp1720I GCTNAGC 1 cut(s) 1059
Bsp19I CCATGG 1 cut(s) 2277
BspACI CCGC 7 cut(s) 186, 189, 317, 650, 863, 1096, 1534
BspANI GGCC 4 cut(s) 292, 1591, 2090, 2378
BspCNI CTCAG 6 cut(s) 773, 1072, 1287, 1594, 1962, 2354
BspFNI CGCG 2 cut(s) 137, 1326
BspHI TCATGA 1 cut(s) 1926
BspLI GGNNCC 8 cut(s) 292, 504, 684, 799, 1387, 1782, 1882, 2353
BspMAI CTGCAG 2 cut(s) 1375, 1938
BspOI GCTAGC 1 cut(s) 1637
BspPI GGATC 5 cut(s) 357, 427, 2261, 2331, 2430
BspT104I TTCGAA 1 cut(s) 2224
BspT107I GGYRCC 2 cut(s) 1385, 1880
BspTI CTTAAG 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 1062
BsrDI GCAATG 4 cut(s) 484, 824, 1038, 2453
BsrFI RCCGGY 1 cut(s) 1201
BsrI ACTGG 4 cut(s) 370, 1306, 1738, 2548
BssAI RCCGGY 1 cut(s) 1201
BssECI CCNNGG 6 cut(s) 498, 897, 2091, 2158, 2277, 2348
BssNI GRCGYC 2 cut(s) 183, 414
BssT1I CCWWGG 5 cut(s) 498, 897, 2091, 2158, 2277
Bst2UI CCWGG 5 cut(s) 697, 1885, 1982, 2349, 2356
Bst4CI ACNGT 5 cut(s) 644, 913, 1125, 1520, 1795
Bst6I CTCTTC 2 cut(s) 552, 570
BstACI GRCGYC 2 cut(s) 183, 414
BstAFI CTTAAG 1 cut(s) 332
BstAPI GCANNNNNTGC 2 cut(s) 1358, 1379
BstBI TTCGAA 1 cut(s) 2224
BstC8I GCNNGC 8 cut(s) 243, 810, 1056, 1384, 1608, 1635, 1869, 2268
BstDEI CTNAG 7 cut(s) 270, 760, 1059, 1295, 1602, 1970, 2341
BstDSI CCRYGG 1 cut(s) 2277
BstF5I GGATG 9 cut(s) 77, 278, 658, 778, 782, 1257, 1489, 1756, 1999
BstFNI CGCG 2 cut(s) 137, 1326
BstMAI GTCTC 3 cut(s) 902, 1062, 1966
BstMCI CGRYCG 2 cut(s) 1342, 1664
BstNI CCWGG 5 cut(s) 697, 1885, 1982, 2349, 2356
BstNSI RCATGY 5 cut(s) 1058, 1240, 1871, 2498, 2630
BstSCI CCNGG 9 cut(s) 287, 292, 504, 516, 695, 1883, 1980, 2347, 2354
BstSFI CTRYAG 2 cut(s) 1371, 1934
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 2 cut(s) 137, 1326
BstV2I GAAGAC 2 cut(s) 18, 1895
BstX2I RGATCY 3 cut(s) 31, 2336, 2422
BstXI CCANNNNNNTGG 2 cut(s) 703, 1637
BstYI RGATCY 3 cut(s) 31, 2336, 2422
BsuRI GGCC 4 cut(s) 292, 1591, 2090, 2378
BtgI CCRYGG 1 cut(s) 2277
BtgZI GCGATG 2 cut(s) 178, 896
BtsCI GGATG 9 cut(s) 77, 278, 658, 778, 782, 1257, 1489, 1756, 1999
BtsI GCAGTG 1 cut(s) 1911
BtsIMutI CAGTG 4 cut(s) 918, 1121, 1516, 1911
Cac8I GCNNGC 8 cut(s) 243, 810, 1056, 1384, 1608, 1635, 1869, 2268
CaiI CAGNNNCTG 3 cut(s) 1358, 1379, 2348
CciI TCATGA 1 cut(s) 1926
Cfr10I RCCGGY 1 cut(s) 1201
Cfr13I GGNCC 7 cut(s) 290, 291, 502, 1589, 2095, 2352, 2377
CseI GACGC 4 cut(s) 191, 422, 1315, 2543
Csp6I GTAC 4 cut(s) 1570, 2026, 2128, 2562
CviQI GTAC 4 cut(s) 1570, 2026, 2128, 2562
DdeI CTNAG 7 cut(s) 270, 760, 1059, 1295, 1602, 1970, 2341
DraI TTTAAA 2 cut(s) 408, 2505
DraIII CACNNNGTG 1 cut(s) 2390
DrdI GACNNNNNNGTC 1 cut(s) 1306
DseDI GACNNNNNNGTC 1 cut(s) 1306
EaeI YGGCCR 1 cut(s) 2088
Eam1104I CTCTTC 2 cut(s) 552, 570
EarI CTCTTC 2 cut(s) 552, 570
Ecl136II GAGCTC 3 cut(s) 46, 838, 918
Eco130I CCWWGG 5 cut(s) 498, 897, 2091, 2158, 2277
Eco24I GRGCYC 4 cut(s) 48, 294, 840, 920
Eco31I GGTCTC 1 cut(s) 1062
Eco47I GGWCC 3 cut(s) 502, 2095, 2352
Eco53kI GAGCTC 3 cut(s) 46, 838, 918
Eco57I CTGAAG 2 cut(s) 594, 2268
Eco88I CYCGRG 2 cut(s) 919, 2298
EcoICRI GAGCTC 3 cut(s) 46, 838, 918
EcoO109I RGGNCCY 4 cut(s) 502, 2095, 2352, 2377
EcoRI GAATTC 2 cut(s) 364, 1181
EcoRII CCWGG 5 cut(s) 695, 1883, 1980, 2347, 2354
EcoT14I CCWWGG 5 cut(s) 498, 897, 2091, 2158, 2277
EcoT22I ATGCAT 1 cut(s) 1056
EcoT38I GRGCYC 4 cut(s) 48, 294, 840, 920
ErhI CCWWGG 5 cut(s) 498, 897, 2091, 2158, 2277
Esp3I CGTCTC 1 cut(s) 1966
FalI AAGNNNNNCTT 2 cut(s) 1768, 1800
FaqI GGGAC 1 cut(s) 2430
FauNDI CATATG 1 cut(s) 1050
FokI GGATG 9 cut(s) 64, 265, 665, 769, 785, 1264, 1496, 1763, 2006
FriOI GRGCYC 4 cut(s) 48, 294, 840, 920
FspBI CTAG 5 cut(s) 1076, 1364, 1634, 2426, 2559
GsaI CCCAGC 3 cut(s) 1041, 1251, 1641
GsuI CTGGAG 3 cut(s) 136, 493, 1755
HaeIII GGCC 4 cut(s) 292, 1591, 2090, 2378
HapII CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
HgaI GACGC 4 cut(s) 191, 422, 1315, 2543
Hin1I GRCGYC 2 cut(s) 183, 414
HindIII AAGCTT 2 cut(s) 713, 1850
HpaII CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
HphI GGTGA 8 cut(s) 80, 598, 985, 1216, 1430, 1568, 2408, 2524
Hpy166II GTNNAC 1 cut(s) 204
Hpy188III TCNNGA 7 cut(s) 1138, 1433, 1688, 1927, 2298, 2305, 2426
Hpy8I GTNNAC 1 cut(s) 204
Hpy99I CGWCG 6 cut(s) 176, 185, 416, 431, 1110, 1860
HpyCH4III ACNGT 5 cut(s) 644, 913, 1125, 1520, 1795
HpyCH4IV ACGT 2 cut(s) 2167, 2389
HpyF3I CTNAG 7 cut(s) 270, 760, 1059, 1295, 1602, 1970, 2341
HpySE526I ACGT 2 cut(s) 2167, 2389
Hsp92I GRCGYC 2 cut(s) 183, 414
LmnI GCTCC 6 cut(s) 43, 474, 1121, 1245, 1451, 1513
LweI GCATC 8 cut(s) 287, 1242, 1345, 1417, 1710, 1762, 1967, 2419
MaeI CTAG 5 cut(s) 1076, 1364, 1634, 2426, 2559
MaeII ACGT 2 cut(s) 2167, 2389
MaeIII GTNAC 5 cut(s) 1098, 1232, 1334, 1556, 1688
MflI RGATCY 3 cut(s) 31, 2336, 2422
MhlI GDGCHC 6 cut(s) 48, 294, 840, 920, 1118, 1518
MlsI TGGCCA 1 cut(s) 2090
MluNI TGGCCA 1 cut(s) 2090
MlyI GAGTC 6 cut(s) 157, 209, 210, 440, 1445, 2310
MmeI TCCRAC 3 cut(s) 354, 775, 1461
Mox20I TGGCCA 1 cut(s) 2090
Mph1103I ATGCAT 1 cut(s) 1056
MroXI GAANNNNTTC 1 cut(s) 2403
MscI TGGCCA 1 cut(s) 2090
MslI CAYNNNNRTG 2 cut(s) 1053, 2619
Msp20I TGGCCA 1 cut(s) 2090
MspA1I CMGCKG 1 cut(s) 1376
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 6 cut(s) 288, 294, 417, 506, 517, 1202
MspR9I CCNGG 9 cut(s) 289, 294, 506, 518, 697, 1885, 1982, 2349, 2356
MssI GTTTAAAC 1 cut(s) 408
Mva1269I GAATGC 2 cut(s) 1290, 1945
MvaI CCWGG 5 cut(s) 697, 1885, 1982, 2349, 2356
MvnI CGCG 2 cut(s) 137, 1326
NciI CCSGG 4 cut(s) 289, 294, 506, 518
NcoI CCATGG 1 cut(s) 2277
NdeI CATATG 1 cut(s) 1050
NheI GCTAGC 1 cut(s) 1633
NlaIV GGNNCC 8 cut(s) 292, 504, 684, 799, 1387, 1782, 1882, 2353
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 3 cut(s) 1334, 1556, 1688
NsiI ATGCAT 1 cut(s) 1056
NspI RCATGY 5 cut(s) 1058, 1240, 1871, 2498, 2630
NspV TTCGAA 1 cut(s) 2224
PaeI GCATGC 2 cut(s) 1058, 1871
PaeR7I CTCGAG 1 cut(s) 2298
PagI TCATGA 1 cut(s) 1926
PciI ACATGT 1 cut(s) 2494
PctI GAATGC 2 cut(s) 1290, 1945
PdmI GAANNNNTTC 1 cut(s) 2403
PfoI TCCNGGA 2 cut(s) 516, 2354
Ple19I CGATCG 2 cut(s) 1342, 1664
PleI GAGTC 6 cut(s) 157, 208, 210, 439, 1444, 2309
PmeI GTTTAAAC 1 cut(s) 408
PpsI GAGTC 6 cut(s) 157, 208, 210, 439, 1444, 2309
PpuMI RGGWCCY 3 cut(s) 502, 2095, 2352
PscI ACATGT 1 cut(s) 2494
PshBI ATTAAT 1 cut(s) 1314
PsiI TTATAA 1 cut(s) 852
Psp124BI GAGCTC 3 cut(s) 48, 840, 920
Psp1406I AACGTT 1 cut(s) 2167
Psp5II RGGWCCY 3 cut(s) 502, 2095, 2352
Psp6I CCWGG 5 cut(s) 695, 1883, 1980, 2347, 2354
PspFI CCCAGC 3 cut(s) 1037, 1247, 1637
PspGI CCWGG 5 cut(s) 695, 1883, 1980, 2347, 2354
PspN4I GGNNCC 8 cut(s) 292, 504, 684, 799, 1387, 1782, 1882, 2353
PspOMI GGGCCC 1 cut(s) 290
PspPI GGNCC 7 cut(s) 290, 291, 502, 1589, 2095, 2352, 2377
PspPPI RGGWCCY 3 cut(s) 502, 2095, 2352
PstI CTGCAG 2 cut(s) 1375, 1938
PstNI CAGNNNCTG 3 cut(s) 1358, 1379, 2348
PsuI RGATCY 3 cut(s) 31, 2336, 2422
PvuI CGATCG 2 cut(s) 1342, 1664
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 4 cut(s) 1571, 2027, 2129, 2563
RsaNI GTAC 4 cut(s) 1570, 2026, 2128, 2562
RseI CAYNNNNRTG 2 cut(s) 1053, 2619
SacI GAGCTC 3 cut(s) 48, 840, 920
Sau96I GGNCC 7 cut(s) 290, 291, 502, 1589, 2095, 2352, 2377
ScaI AGTACT 1 cut(s) 2027
SchI GAGTC 6 cut(s) 157, 209, 210, 440, 1445, 2310
ScrFI CCNGG 9 cut(s) 289, 294, 506, 518, 697, 1885, 1982, 2349, 2356
SduI GDGCHC 6 cut(s) 48, 294, 840, 920, 1118, 1518
SfaNI GCATC 8 cut(s) 287, 1242, 1345, 1417, 1710, 1762, 1967, 2419
SfcI CTRYAG 2 cut(s) 1371, 1934
Sfr274I CTCGAG 1 cut(s) 2298
SfuI TTCGAA 1 cut(s) 2224
SinI GGWCC 3 cut(s) 502, 2095, 2352
SlaI CTCGAG 1 cut(s) 2298
SmiMI CAYNNNNRTG 2 cut(s) 1053, 2619
SmlI CTYRAG 6 cut(s) 225, 332, 1433, 2298, 2459, 2590
SmoI CTYRAG 6 cut(s) 225, 332, 1433, 2298, 2459, 2590
SpeI ACTAGT 1 cut(s) 1075
SphI GCATGC 2 cut(s) 1058, 1871
SsiI CCGC 7 cut(s) 186, 189, 317, 650, 863, 1096, 1534
SspI AATATT 2 cut(s) 1833, 2447
SspMI CTAG 5 cut(s) 1076, 1364, 1634, 2426, 2559
SstI GAGCTC 3 cut(s) 48, 840, 920
StyD4I CCNGG 9 cut(s) 287, 292, 504, 516, 695, 1883, 1980, 2347, 2354
StyI CCWWGG 5 cut(s) 498, 897, 2091, 2158, 2277
TaaI ACNGT 5 cut(s) 644, 913, 1125, 1520, 1795
TaiI ACGT 2 cut(s) 2170, 2392
TaqI TCGA 9 cut(s) 352, 434, 639, 1310, 1446, 1855, 2138, 2224, 2299
TatI WGTACW 1 cut(s) 2025
TauI GCSGC 2 cut(s) 188, 191
TscAI CASTG 4 cut(s) 918, 1128, 1523, 1918
TseFI GTSAC 3 cut(s) 1334, 1556, 1688
Tsp45I GTSAC 3 cut(s) 1334, 1556, 1688
TspGWI ACGGA 3 cut(s) 160, 1094, 1798
TspRI CASTG 4 cut(s) 918, 1128, 1523, 1918
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 3 cut(s) 502, 2095, 2352
VspI ATTAAT 1 cut(s) 1314
XapI RAATTY 7 cut(s) 364, 544, 635, 1181, 2219, 2399, 2537
XbaI TCTAGA 1 cut(s) 2425
XceI RCATGY 5 cut(s) 1058, 1240, 1871, 2498, 2630
XhoI CTCGAG 1 cut(s) 2298
XmnI GAANNNNTTC 1 cut(s) 2403
XspI CTAG 5 cut(s) 1076, 1364, 1634, 2426, 2559
ZrmI AGTACT 1 cut(s) 2027
Zsp2I ATGCAT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.