MD14G1054400.v1.1

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0017505
Physical Location & Seq
Forward (+)
5488896 .. 5492143
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1054400.v1.1.491

Sequence Viewer

Length: 2568 bp
ATGGAGGAAGAAGACCAGCAAAAAGTTGCAGATCTGATCAAAGAGCTCGTCATCCGCCTCCGCTCCCAAAACCCCAATTCCGAATCTCACCCACCGACTCCAAACTCCCCGGAATTCCAGAGCTCGCTTCGCTACGCTTTCCGCTTGATCTCCAGCCGATTAAATCCGTCCGTGGCTCCCGACGCCACCGCCATAGCCGAGTCCACCAAGCGCCGCCTCGCCACTCAAGGTAAGTCCTCGCAAGCTCTCACTTTTGCCGACCTTTATACCAAATTCGCGTCCAAAACCGGACCAGGGAGTGTAAATAACAAATGGGCAGTCGTTTATTTGCTTAAAATTATATCGGAGGATCGGAAAAATGGGAAGACACAGTTGGATTCTTCGGTTTTGTTGCCGAATTTGGGGTTTAATGATGCCGAATCGGGGAAGGAATCGAGGGTTTTGGTGGGTAGGGGAAATAAGGAAAAGGGTTGGAATAATGGGGTTTTGTTGGTTTCTAAAGACCCGGAAAATCTTCGTGAAATTGCGTTTAGGGAGTATGTGAATTTGATTAAAGAAGAGAATGAAGTTTCTGAAGAGGTTTTGGTGAGAGATGTGTTGTATGCCTGCCAAGGAATTGATGGAAAGTATGTGAAGTTTGATAGTAGTGCTGATGGTTATGCTTTATCTGATCTGATTAAGGTGCCTCGAGCAACCAGCATTATGGTTCGGAAGCTTTGTGAATTGGGGTGGTTGTTTAGGAAGGTTAAAGGGTATATTTCAGAGAGTATGGATGGGTTTCCATCTGAAGACGTTGGAACCATCGGGCAGGCCTTTTGTGCTGCATTGCAAGATGAGCTCTCCGATTATTATAAGTTGTTGGCAGTGCTTGAAGCACAGTCGATGAATCCGATTCCATTGGTTTCAGAGACAGCGAGCTCAGGGAATTATCTTTCGCTGAGGAGATTGTCTGTTTGGTTTGCCGAGCCAATGGTGAAAATGCGGTTGATGGCTGTTTTGGTTGATAAATGTAGGGTCTTAAGAGGTGGGGCAATGGCTGGGGCTATTCATTTGCATGCCCAACATGGTGACCCATTGGTGCACGAGTTCATGGGACGTTTACTTCGGAGGGTGTGTTCTCCTCTATTTGAGATGGTGAGGAGTTGGGTTTTGGAAGGGGAGTTAGAAGATGTTTTTGCAGAATTCTTTGTAGTGGGCCAGCCAGTGAAAGCTGAGTCCCTTTGGAGGGAGGGTTACATGCTCCATGCCGGAATGCTTCCTTCTTTTATTTCACAATCTCTTGCTCAACGCATCCTGAGGACAGGCAAGTCAATCAATTTCCTTCGTGTCTGTTGTGAGGATCGTGGTTGGGCTGATGCTGCAACAGAAGCTGCAGCTGCTGCTGGTACCACAACAAAAAGATGGGGTCTTGGATATGGTGAAAGTGATGCTCTTGAGTCTTTGGTGGATGGAGCAGCAAAGAGAGTTGATAAGCATTTGTTGGATGTTATTTACAACCAGTATAAGTTCAAGGAGCACTGTCTTGCAATAAAGCGGTATTTACTCCTTGGACAGGGTGACTTTGTTCAGTATCTAATGGATATTGTTGGGCCTGAGCTCTCTGAACCTGCTAACACTATAAGCTCTTTTCAGCTAGCTGGATTGCTTGAAACTGCAATTCGAGCATCTAATGCTCAGTATGATGATCGTGACATATTGGATAGGTTGAAGGTAAAGATGATGCCACATGGAACCGGAGATAGGGGCTGGGATGTGTTCTCATTGGAATATGATGCAAGGGTTCCTTTAGATACTGTTTTTACAGAGTCTGTGATGACAAAGTATTTAAGAATTTTTAATTTCTTGTGGAAGCTTCGGCGAGTAGAGCATGCCCTTATTGGTATTTGGAAGACAATGAAGCCAAATTGTATCACCTCTCGTTCATTTATGAAGCTTCCCCATGCAGTTAAGTTGCAGTTACTTTCAACTCTGAGACGATGCCAGGTTCTTTGGGATGAAATGAATCATTTTGTCTCAAACTTGCAGTACTATATTATGTTTGAAGTCTTGGAGGTGTCCTGGTCCCATTTCTTGGACGAAATGGAGGTGGCCAAGGACCTTGATGATCTACTTGCTGCACATGAAAAGTACCTCCATTCAATTGTGGAGAAATCTCTCCTTGGAGAACGGTCCCAAACCCTTTACAATTCACTCTTTGCCTTGTTTGATCTTATACTGAAATTCCGAAGTCATGCAGATCGATTGTCTGAAGGGATTAATGAGTTGCAAGCAAGAACCATGGAATCCTCTTTACCCTCTCGAGACAAGAGTAAAACAAAAAAGCGATCAAATGATAGGTCCTCAGAGCCTGGATCCTGGATTAGTGAAGGCAGGAAAGCCCTCACACAACGTGCTGGTGAATTTCTTCGGAATATGGGACAAGATTTAGACACACTATCAAAAGAATATTCATCATTGCTTGAGGATTTTATTTCCAAGTTACCCATGCAACAACATGTTGATTTAAAGTTCCTCCTGTTTCGGCTTGACTTCACTGAGTTTTATAGCCAGTTGCGTCCTAGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

856

Amino Acids

96.92

Weight (kDa)

6.54

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCP_N_terminal PF17681 196 - 499 3e-77 Gamma tubulin complex component N-terminal
GCP_C_terminal PF04130 505 - 848 3e-81 Gamma tubulin complex component C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06680
fragaria_vesca FvH4_6g21970
malus_domestica MD12G1055200.v1.1 MD14G1054400.v1.1
prunus_persica Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1
pyrus_communis pycom12g04820 pycom14g04490
rosa_laevigata RLG00000023588
rosa_roxburghii Rroxscaffold_6G00402450
rosa_samantha Rh3CG253200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
AasI GACNNNNNNGTC 1 cut(s) 1306
Acc36I ACCTGC 1 cut(s) 1615
Acc65I GGTACC 1 cut(s) 1385
AccB1I GGYRCC 2 cut(s) 682, 1385
AccB7I CCANNNNNTGG 1 cut(s) 2071
AccBSI CCGCTC 1 cut(s) 63
AccII CGCG 1 cut(s) 278
AciI CCGC 7 cut(s) 55, 61, 142, 189, 214, 982, 1534
AclWI GGATC 4 cut(s) 357, 1347, 2346, 2359
AcoI YGGCCR 1 cut(s) 2088
AcsI RAATTY 8 cut(s) 113, 272, 397, 544, 1181, 1830, 2219, 2399
AcuI CTGAAG 3 cut(s) 594, 807, 2268
AcyI GRCGYC 1 cut(s) 183
AdeI CACNNNGTG 1 cut(s) 2390
AfaI GTAC 4 cut(s) 1387, 2027, 2129, 2563
AfiI CCNNNNNNNGG 8 cut(s) 294, 401, 423, 1224, 1225, 1552, 1740, 2071
AflII CTTAAG 1 cut(s) 1018
AflIII ACRYGT 1 cut(s) 2494
AgsI TTSAA 7 cut(s) 872, 1510, 1649, 1708, 1965, 2042, 2139
AjnI CCWGG 5 cut(s) 292, 1980, 2057, 2347, 2354
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
AloI GAACNNNNNNTCC 2 cut(s) 1099, 1131
Alw21I GWGCWC 7 cut(s) 48, 125, 840, 920, 1083, 1518, 1599
Alw26I GTCTC 4 cut(s) 902, 1966, 2017, 2295
Alw44I GTGCAC 1 cut(s) 1079
AlwI GGATC 4 cut(s) 357, 1347, 2346, 2359
AlwNI CAGNNNCTG 4 cut(s) 1370, 1379, 1808, 2348
Ama87I CYCGRG 2 cut(s) 687, 2298
AoxI GGCC 4 cut(s) 810, 1195, 1589, 2088
ApaLI GTGCAC 1 cut(s) 1079
ApeKI GCWGC 8 cut(s) 821, 1358, 1370, 1373, 1376, 1379, 1454, 2114
ApoI RAATTY 8 cut(s) 113, 272, 397, 544, 1181, 1830, 2219, 2399
ArsI GACNNNNNNTTYG 8 cut(s) 33, 65, 303, 335, 1086, 1118, 2321, 2353
AseI ATTAAT 1 cut(s) 2256
Asp700I GAANNNNTTC 2 cut(s) 513, 2403
Asp718I GGTACC 1 cut(s) 1385
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 7 cut(s) 290, 1195, 1589, 2061, 2095, 2169, 2337
AsuC2I CCSGG 2 cut(s) 110, 506
AsuHPI GGTGA 9 cut(s) 80, 598, 985, 1079, 1147, 1430, 1568, 1903, 2408
AsuNHI GCTAGC 1 cut(s) 1633
AvaI CYCGRG 2 cut(s) 687, 2298
AvaII GGWCC 5 cut(s) 290, 2061, 2095, 2169, 2337
AxyI CCTNAGG 1 cut(s) 1295
BaeGI GKGCMC 1 cut(s) 1083
BalI TGGCCA 1 cut(s) 2090
BamHI GGATCC 1 cut(s) 2351
BanI GGYRCC 2 cut(s) 682, 1385
BanII GRGCYC 5 cut(s) 48, 125, 840, 920, 1599
BauI CACGAG 1 cut(s) 1082
BbsI GAAGAC 4 cut(s) 18, 371, 795, 1895
Bbv12I GWGCWC 7 cut(s) 48, 125, 840, 920, 1083, 1518, 1599
BbvCI CCTCAGC 1 cut(s) 938
BbvI GCAGC 8 cut(s) 808, 1345, 1357, 1363, 1366, 1385, 1466, 2101
BccI CCATC 9 cut(s) 614, 647, 767, 790, 809, 982, 1126, 1395, 1442
BciT130I CCWGG 5 cut(s) 294, 1982, 2059, 2349, 2356
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 2 cut(s) 110, 506
BcoDI GTCTC 4 cut(s) 902, 1966, 2017, 2295
BfaI CTAG 2 cut(s) 1634, 2559
BfmI CTRYAG 1 cut(s) 1371
BfoI RGCGCY 1 cut(s) 214
BfrI CTTAAG 1 cut(s) 1018
BfuAI ACCTGC 1 cut(s) 1615
BglII AGATCT 1 cut(s) 31
BisI GCNGC 9 cut(s) 214, 822, 1359, 1371, 1374, 1377, 1380, 1455, 2115
BlsI GCNGC 9 cut(s) 215, 823, 1360, 1372, 1375, 1378, 1381, 1456, 2116
BmcAI AGTACT 1 cut(s) 2027
Bme1390I CCNGG 7 cut(s) 110, 294, 506, 1982, 2059, 2349, 2356
Bme18I GGWCC 5 cut(s) 290, 2061, 2095, 2169, 2337
BmeT110I CYCGRG 2 cut(s) 687, 2298
BmgT120I GGNCC 7 cut(s) 290, 1195, 1589, 2061, 2095, 2169, 2337
BmiI GGNNCC 9 cut(s) 177, 684, 799, 1387, 1732, 1782, 2063, 2171, 2353
BmrFI CCNGG 7 cut(s) 110, 294, 506, 1982, 2059, 2349, 2356
BmsI GCATC 8 cut(s) 403, 1299, 1345, 1417, 1673, 1710, 1762, 1967
BmtI GCTAGC 1 cut(s) 1637
BpiI GAAGAC 4 cut(s) 18, 371, 795, 1895
BpmI CTGGAG 1 cut(s) 136
Bpu10I CCTNAGC 3 cut(s) 919, 938, 1593
BpuEI CTTGAG 3 cut(s) 210, 1454, 2480
BpuMI CCSGG 2 cut(s) 110, 506
Bsa29I ATCGAT 1 cut(s) 2239
BsaAI YACGTR 1 cut(s) 2565
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 8 cut(s) 108, 171, 293, 610, 1546, 2091, 2158, 2277
BsaWI WCCGGW 2 cut(s) 287, 1733
BsaXI ACNNNNNCTCC 4 cut(s) 1099, 1129, 1216, 1246
Bsc4I CCNNNNNNNGG 8 cut(s) 294, 401, 423, 1224, 1225, 1552, 1740, 2071
Bse1I ACTGG 3 cut(s) 1202, 1498, 2548
Bse21I CCTNAGG 1 cut(s) 1295
Bse3DI GCAATG 3 cut(s) 824, 1038, 2453
BseBI CCWGG 5 cut(s) 294, 1982, 2059, 2349, 2356
BseCI ATCGAT 1 cut(s) 2239
BseDI CCNNGG 8 cut(s) 108, 171, 293, 610, 1546, 2091, 2158, 2277
BseGI GGATG 7 cut(s) 51, 778, 1290, 1453, 1489, 1756, 1999
BseLI CCNNNNNNNGG 8 cut(s) 294, 401, 423, 1224, 1225, 1552, 1740, 2071
BseMI GCAATG 3 cut(s) 824, 1038, 2453
BseMII CTCAG 9 cut(s) 929, 933, 1203, 1286, 1584, 1688, 1961, 2355, 2526
BseNI ACTGG 3 cut(s) 1202, 1498, 2548
BseRI GAGGAG 3 cut(s) 955, 1110, 1153
BseSI GKGCMC 1 cut(s) 1083
BseXI GCAGC 8 cut(s) 808, 1345, 1357, 1363, 1366, 1385, 1466, 2101
BseYI CCCAGC 2 cut(s) 1037, 1746
BsgI GTGCAG 1 cut(s) 2100
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 4 cut(s) 812, 1197, 1591, 2090
BshNI GGYRCC 2 cut(s) 682, 1385
BshVI ATCGAT 1 cut(s) 2239
BsiHKAI GWGCWC 7 cut(s) 48, 125, 840, 920, 1083, 1518, 1599
BsiHKCI CYCGRG 2 cut(s) 687, 2298
BsiSI CCGG 5 cut(s) 110, 288, 506, 1248, 1734
BslFI GGGAC 5 cut(s) 1107, 1201, 2047, 2155, 2430
BslI CCNNNNNNNGG 8 cut(s) 294, 401, 423, 1224, 1225, 1552, 1740, 2071
BsmAI GTCTC 4 cut(s) 902, 1966, 2017, 2295
BsmBI CGTCTC 1 cut(s) 1966
BsmFI GGGAC 5 cut(s) 1107, 1201, 2047, 2155, 2430
BsmI GAATGC 1 cut(s) 1257
BsnI GGCC 4 cut(s) 812, 1197, 1591, 2090
BsoBI CYCGRG 2 cut(s) 687, 2298
Bsp1286I GDGCHC 7 cut(s) 48, 125, 840, 920, 1083, 1518, 1599
Bsp19I CCATGG 1 cut(s) 2277
BspACI CCGC 7 cut(s) 55, 61, 142, 189, 214, 982, 1534
BspANI GGCC 4 cut(s) 812, 1197, 1591, 2090
BspCNI CTCAG 9 cut(s) 930, 932, 1204, 1287, 1585, 1687, 1962, 2354, 2527
BspDI ATCGAT 1 cut(s) 2239
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 9 cut(s) 177, 684, 799, 1387, 1732, 1782, 2063, 2171, 2353
BspMAI CTGCAG 1 cut(s) 1375
BspMI ACCTGC 1 cut(s) 1615
BspOI GCTAGC 1 cut(s) 1637
BspPI GGATC 4 cut(s) 357, 1347, 2346, 2359
BspT107I GGYRCC 2 cut(s) 682, 1385
BspTI CTTAAG 1 cut(s) 1018
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 3 cut(s) 824, 1038, 2453
BsrI ACTGG 3 cut(s) 1202, 1498, 2548
BssECI CCNNGG 8 cut(s) 108, 171, 293, 610, 1546, 2091, 2158, 2277
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 1082
BssT1I CCWWGG 5 cut(s) 610, 1546, 2091, 2158, 2277
Bst2BI CACGAG 1 cut(s) 1082
Bst2UI CCWGG 5 cut(s) 294, 1982, 2059, 2349, 2356
Bst4CI ACNGT 5 cut(s) 372, 879, 1520, 1795, 2169
Bst6I CTCTTC 2 cut(s) 552, 570
BstACI GRCGYC 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 1018
BstAPI GCANNNNNTGC 2 cut(s) 1379, 1670
BstBAI YACGTR 1 cut(s) 2565
BstDEI CTNAG 9 cut(s) 919, 938, 1212, 1295, 1593, 1674, 1970, 2341, 2535
BstDSI CCRYGG 2 cut(s) 171, 2277
BstEII GGTNACC 1 cut(s) 1067
BstENI CCTNNNNNAGG 1 cut(s) 1550
BstF5I GGATG 7 cut(s) 51, 778, 1290, 1453, 1489, 1756, 1999
BstFNI CGCG 1 cut(s) 278
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstMAI GTCTC 4 cut(s) 902, 1966, 2017, 2295
BstNI CCWGG 5 cut(s) 294, 1982, 2059, 2349, 2356
BstNSI RCATGY 4 cut(s) 1058, 1240, 1871, 2498
BstPI GGTNACC 1 cut(s) 1067
BstSCI CCNGG 7 cut(s) 108, 292, 504, 1980, 2057, 2347, 2354
BstSFI CTRYAG 1 cut(s) 1371
BstSLI GKGCMC 1 cut(s) 1083
BstSNI TACGTA 1 cut(s) 2565
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 8 cut(s) 808, 1345, 1357, 1363, 1366, 1385, 1466, 2101
BstV2I GAAGAC 4 cut(s) 18, 371, 795, 1895
BstX2I RGATCY 2 cut(s) 31, 2351
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 2 cut(s) 31, 2351
Bsu15I ATCGAT 1 cut(s) 2239
Bsu36I CCTNAGG 1 cut(s) 1295
BsuRI GGCC 4 cut(s) 812, 1197, 1591, 2090
BsuTUI ATCGAT 1 cut(s) 2239
BtgI CCRYGG 2 cut(s) 171, 2277
BtsCI GGATG 7 cut(s) 51, 778, 1290, 1453, 1489, 1756, 1999
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 4 cut(s) 870, 1209, 1516, 2532
BveI ACCTGC 1 cut(s) 1615
CaiI CAGNNNCTG 4 cut(s) 1370, 1379, 1808, 2348
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 7 cut(s) 290, 1195, 1589, 2061, 2095, 2169, 2337
ClaI ATCGAT 1 cut(s) 2239
CseI GACGC 3 cut(s) 191, 267, 2543
Csp6I GTAC 4 cut(s) 1386, 2026, 2128, 2562
CviQI GTAC 4 cut(s) 1386, 2026, 2128, 2562
DdeI CTNAG 9 cut(s) 919, 938, 1212, 1295, 1593, 1674, 1970, 2341, 2535
DraI TTTAAA 1 cut(s) 2505
DraIII CACNNNGTG 1 cut(s) 2390
DrdI GACNNNNNNGTC 1 cut(s) 1306
DseDI GACNNNNNNGTC 1 cut(s) 1306
EaeI YGGCCR 1 cut(s) 2088
Eam1104I CTCTTC 2 cut(s) 552, 570
EarI CTCTTC 2 cut(s) 552, 570
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 5 cut(s) 46, 123, 838, 918, 1597
Eco105I TACGTA 1 cut(s) 2565
Eco130I CCWWGG 5 cut(s) 610, 1546, 2091, 2158, 2277
Eco147I AGGCCT 1 cut(s) 812
Eco24I GRGCYC 5 cut(s) 48, 125, 840, 920, 1599
Eco47I GGWCC 5 cut(s) 290, 2061, 2095, 2169, 2337
Eco53kI GAGCTC 5 cut(s) 46, 123, 838, 918, 1597
Eco57I CTGAAG 3 cut(s) 594, 807, 2268
Eco81I CCTNAGG 1 cut(s) 1295
Eco88I CYCGRG 2 cut(s) 687, 2298
Eco91I GGTNACC 1 cut(s) 1067
EcoICRI GAGCTC 5 cut(s) 46, 123, 838, 918, 1597
EcoNI CCTNNNNNAGG 1 cut(s) 1550
EcoO109I RGGNCCY 2 cut(s) 2095, 2337
EcoO65I GGTNACC 1 cut(s) 1067
EcoRI GAATTC 2 cut(s) 113, 1181
EcoRII CCWGG 5 cut(s) 292, 1980, 2057, 2347, 2354
EcoT14I CCWWGG 5 cut(s) 610, 1546, 2091, 2158, 2277
EcoT38I GRGCYC 5 cut(s) 48, 125, 840, 920, 1599
ErhI CCWWGG 5 cut(s) 610, 1546, 2091, 2158, 2277
Esp3I CGTCTC 1 cut(s) 1966
FalI AAGNNNNNCTT 2 cut(s) 1768, 1800
FaqI GGGAC 5 cut(s) 1107, 1201, 2047, 2155, 2430
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 9 cut(s) 214, 822, 1359, 1371, 1374, 1377, 1380, 1455, 2115
FokI GGATG 7 cut(s) 38, 785, 1277, 1460, 1496, 1763, 2006
FriOI GRGCYC 5 cut(s) 48, 125, 840, 920, 1599
Fsp4HI GCNGC 9 cut(s) 214, 822, 1359, 1371, 1374, 1377, 1380, 1455, 2115
FspBI CTAG 2 cut(s) 1634, 2559
GlaI GCGC 1 cut(s) 212
GluI GCNGC 9 cut(s) 214, 822, 1359, 1371, 1374, 1377, 1380, 1455, 2115
GsaI CCCAGC 2 cut(s) 1041, 1750
GsuI CTGGAG 1 cut(s) 136
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 4 cut(s) 812, 1197, 1591, 2090
HapII CCGG 5 cut(s) 110, 288, 506, 1248, 1734
HgaI GACGC 3 cut(s) 191, 267, 2543
HhaI GCGC 1 cut(s) 213
Hin1I GRCGYC 1 cut(s) 183
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HindIII AAGCTT 3 cut(s) 713, 1850, 1931
HpaII CCGG 5 cut(s) 110, 288, 506, 1248, 1734
HphI GGTGA 9 cut(s) 80, 598, 985, 1079, 1147, 1430, 1568, 1903, 2408
Hpy166II GTNNAC 3 cut(s) 204, 1081, 1100
Hpy188III TCNNGA 8 cut(s) 118, 179, 518, 1294, 1433, 1688, 2298, 2300
Hpy8I GTNNAC 3 cut(s) 204, 1081, 1100
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 8 cut(s) 421, 736, 1148, 1269, 1331, 1702, 2243, 2360
HpyCH4III ACNGT 5 cut(s) 372, 879, 1520, 1795, 2169
HpyCH4IV ACGT 4 cut(s) 792, 1096, 2389, 2564
HpyF3I CTNAG 9 cut(s) 919, 938, 1212, 1295, 1593, 1674, 1970, 2341, 2535
HpySE526I ACGT 4 cut(s) 792, 1096, 2389, 2564
Hsp92I GRCGYC 1 cut(s) 183
HspAI GCGC 1 cut(s) 211
KpnI GGTACC 1 cut(s) 1389
Ksp22I TGATCA 1 cut(s) 36
LmnI GCTCC 5 cut(s) 68, 181, 1245, 1451, 1513
Lsp1109I GCAGC 8 cut(s) 808, 1345, 1357, 1363, 1366, 1385, 1466, 2101
LweI GCATC 8 cut(s) 403, 1299, 1345, 1417, 1673, 1710, 1762, 1967
MaeI CTAG 2 cut(s) 1634, 2559
MaeII ACGT 4 cut(s) 792, 1096, 2389, 2564
MaeIII GTNAC 6 cut(s) 1067, 1232, 1556, 1688, 1956, 2478
MbiI CCGCTC 1 cut(s) 63
MfeI CAATTG 1 cut(s) 2139
MflI RGATCY 2 cut(s) 31, 2351
MhlI GDGCHC 7 cut(s) 48, 125, 840, 920, 1083, 1518, 1599
MlsI TGGCCA 1 cut(s) 2090
MluNI TGGCCA 1 cut(s) 2090
MlyI GAGTC 5 cut(s) 91, 209, 1223, 1445, 1814
MmeI TCCRAC 4 cut(s) 354, 452, 775, 1461
Mox20I TGGCCA 1 cut(s) 2090
MroXI GAANNNNTTC 2 cut(s) 513, 2403
MscI TGGCCA 1 cut(s) 2090
MslI CAYNNNNRTG 1 cut(s) 1053
Msp20I TGGCCA 1 cut(s) 2090
MspA1I CMGCKG 1 cut(s) 1376
MspCI CTTAAG 1 cut(s) 1018
MspI CCGG 5 cut(s) 110, 288, 506, 1248, 1734
MspR9I CCNGG 7 cut(s) 110, 294, 506, 1982, 2059, 2349, 2356
MunI CAATTG 1 cut(s) 2139
Mva1269I GAATGC 1 cut(s) 1257
MvaI CCWGG 5 cut(s) 294, 1982, 2059, 2349, 2356
MvnI CGCG 1 cut(s) 278
NciI CCSGG 2 cut(s) 110, 506
NcoI CCATGG 1 cut(s) 2277
NheI GCTAGC 1 cut(s) 1633
NlaIV GGNNCC 9 cut(s) 177, 684, 799, 1387, 1732, 1782, 2063, 2171, 2353
NmeAIII GCCGAG 2 cut(s) 223, 988
NmuCI GTSAC 3 cut(s) 1067, 1556, 1688
NspI RCATGY 4 cut(s) 1058, 1240, 1871, 2498
PaeI GCATGC 2 cut(s) 1058, 1871
PaeR7I CTCGAG 2 cut(s) 687, 2298
PceI AGGCCT 1 cut(s) 812
PciI ACATGT 1 cut(s) 2494
PcsI WCGNNNNNNNCGW 1 cut(s) 887
PctI GAATGC 1 cut(s) 1257
PdmI GAANNNNTTC 2 cut(s) 513, 2403
PfeI GAWTC 8 cut(s) 83, 377, 419, 431, 886, 892, 2002, 2282
PflMI CCANNNNNTGG 1 cut(s) 2071
PfoI TCCNGGA 1 cut(s) 2354
PkrI GCNGC 9 cut(s) 215, 823, 1360, 1372, 1375, 1378, 1381, 1456, 2116
PleI GAGTC 5 cut(s) 91, 208, 1222, 1444, 1813
PpsI GAGTC 5 cut(s) 91, 208, 1222, 1444, 1813
Ppu21I YACGTR 1 cut(s) 2565
PpuMI RGGWCCY 2 cut(s) 2095, 2337
PscI ACATGT 1 cut(s) 2494
PshBI ATTAAT 1 cut(s) 2256
PsiI TTATAA 1 cut(s) 852
Psp124BI GAGCTC 5 cut(s) 48, 125, 840, 920, 1599
Psp5II RGGWCCY 2 cut(s) 2095, 2337
Psp6I CCWGG 5 cut(s) 292, 1980, 2057, 2347, 2354
PspEI GGTNACC 1 cut(s) 1067
PspFI CCCAGC 2 cut(s) 1037, 1746
PspGI CCWGG 5 cut(s) 292, 1980, 2057, 2347, 2354
PspN4I GGNNCC 9 cut(s) 177, 684, 799, 1387, 1732, 1782, 2063, 2171, 2353
PspPI GGNCC 7 cut(s) 290, 1195, 1589, 2061, 2095, 2169, 2337
PspPPI RGGWCCY 2 cut(s) 2095, 2337
PspXI VCTCGAGB 1 cut(s) 687
PstI CTGCAG 1 cut(s) 1375
PstNI CAGNNNCTG 4 cut(s) 1370, 1379, 1808, 2348
PsuI RGATCY 2 cut(s) 31, 2351
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 4 cut(s) 1387, 2027, 2129, 2563
RsaNI GTAC 4 cut(s) 1386, 2026, 2128, 2562
RseI CAYNNNNRTG 1 cut(s) 1053
SacI GAGCTC 5 cut(s) 48, 125, 840, 920, 1599
SatI GCNGC 9 cut(s) 214, 822, 1359, 1371, 1374, 1377, 1380, 1455, 2115
Sau96I GGNCC 7 cut(s) 290, 1195, 1589, 2061, 2095, 2169, 2337
ScaI AGTACT 1 cut(s) 2027
SchI GAGTC 5 cut(s) 91, 209, 1223, 1445, 1814
ScrFI CCNGG 7 cut(s) 110, 294, 506, 1982, 2059, 2349, 2356
SduI GDGCHC 7 cut(s) 48, 125, 840, 920, 1083, 1518, 1599
SfaNI GCATC 8 cut(s) 403, 1299, 1345, 1417, 1673, 1710, 1762, 1967
SfcI CTRYAG 1 cut(s) 1371
Sfr274I CTCGAG 2 cut(s) 687, 2298
SinI GGWCC 5 cut(s) 290, 2061, 2095, 2169, 2337
SlaI CTCGAG 2 cut(s) 687, 2298
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 6 cut(s) 225, 687, 1018, 1433, 2298, 2459
SmoI CTYRAG 6 cut(s) 225, 687, 1018, 1433, 2298, 2459
SnaBI TACGTA 1 cut(s) 2565
SphI GCATGC 2 cut(s) 1058, 1871
SseBI AGGCCT 1 cut(s) 812
SsiI CCGC 7 cut(s) 55, 61, 142, 189, 214, 982, 1534
SspI AATATT 1 cut(s) 2447
SspMI CTAG 2 cut(s) 1634, 2559
SstI GAGCTC 5 cut(s) 48, 125, 840, 920, 1599
StuI AGGCCT 1 cut(s) 812
StyD4I CCNGG 7 cut(s) 108, 292, 504, 1980, 2057, 2347, 2354
StyI CCWWGG 5 cut(s) 610, 1546, 2091, 2158, 2277
TaaI ACNGT 5 cut(s) 372, 879, 1520, 1795, 2169
TaiI ACGT 4 cut(s) 795, 1099, 2392, 2567
TaqI TCGA 6 cut(s) 434, 688, 881, 1660, 2239, 2299
TatI WGTACW 1 cut(s) 2025
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 8 cut(s) 83, 377, 419, 431, 886, 892, 2002, 2282
TscAI CASTG 4 cut(s) 870, 1209, 1523, 2539
TseFI GTSAC 3 cut(s) 1067, 1556, 1688
TseI GCWGC 8 cut(s) 821, 1358, 1370, 1373, 1376, 1379, 1454, 2114
Tsp45I GTSAC 3 cut(s) 1067, 1556, 1688
TspGWI ACGGA 2 cut(s) 156, 160
TspRI CASTG 4 cut(s) 870, 1209, 1523, 2539
Van91I CCANNNNNTGG 1 cut(s) 2071
Vha464I CTTAAG 1 cut(s) 1018
VneI GTGCAC 1 cut(s) 1079
VpaK11BI GGWCC 5 cut(s) 290, 2061, 2095, 2169, 2337
VspI ATTAAT 1 cut(s) 2256
XagI CCTNNNNNAGG 1 cut(s) 1550
XapI RAATTY 8 cut(s) 113, 272, 397, 544, 1181, 1830, 2219, 2399
XceI RCATGY 4 cut(s) 1058, 1240, 1871, 2498
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XhoI CTCGAG 2 cut(s) 687, 2298
XmnI GAANNNNTTC 2 cut(s) 513, 2403
XspI CTAG 2 cut(s) 1634, 2559
ZrmI AGTACT 1 cut(s) 2027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.