FvH4_6g26570

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0018058
Physical Location & Seq
Reverse (-)
20292836 .. 20293264
429 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26570.t1

Sequence Viewer

Length: 429 bp
ATGTTGCTCTACAAGCAGAAGTCCAAGGAAGTCAAGGGCGACAATAATATGATGCTGATCAGAATCAGTGTGGTGGGCAGTACCGGGCCGATCCGGTTCGTGGTGAACAAGAAGGAACTAGTCGGTGTGGTCATCCAAAGGGCCTTGAAATCCTACGAGCGTGACGGTCGGCTACCAGTTCTTGGCTCCGATCTCAAAGATTTTGTGCTATACTGCACTACTGCCGCCGGCACTGATGCTCTGAGTCCGTGGGAGACAATTGGATCCCGGGAGGGAGTTAACTTCACTCTATGCAAGAAGCCACCTACGACTCAGCCAATGCCATTGGCTGCAGCTGCTTCTGGTCACAAGAAGAGTTTGATCAAGGGCTGGATCAACAAGTCCCTCAACTTATTTAGAGAATTCCAATGGGATTTGATTGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

15.78

Weight (kDa)

9.66

Isoelectric Point (pI)

26.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 16 - 98 2.6e-21 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26570
rosa_chinensis RchiOBHm_Chr2g0127581
rosa_laevigata RLG00000018953
rosa_multiflora Rmu_sc0004744.1_g000002
rosa_roxburghii Rroxscaffold_2G00116520
rosa_rugosa Rorug02G0273000
rosa_samantha Rh2BG334300 Rh2CG312300 Rh2CG312500 Rh2DG352400
rosa_wichuraiana Rw2G026380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 182
AciI CCGC 1 cut(s) 225
AclWI GGATC 4 cut(s) 85, 258, 271, 380
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 100, 182
AgsI TTSAA 1 cut(s) 148
AhlI ACTAGT 1 cut(s) 118
AluBI AGCT 1 cut(s) 335
AluI AGCT 1 cut(s) 335
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 4 cut(s) 85, 258, 271, 380
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 2 cut(s) 86, 141
ApeKI GCWGC 3 cut(s) 329, 332, 335
ApoI RAATTY 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 86, 141
AsuC2I CCSGG 3 cut(s) 85, 268, 269
AsuHPI GGTGA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 267
BamHI GGATCC 1 cut(s) 263
BbvI GCAGC 3 cut(s) 316, 322, 344
BclI TGATCA 2 cut(s) 57, 360
BcnI CCSGG 3 cut(s) 85, 268, 269
BcoDI GTCTC 1 cut(s) 248
BcuI ACTAGT 1 cut(s) 118
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 4 cut(s) 225, 330, 333, 336
BlsI GCNGC 4 cut(s) 226, 331, 334, 337
Bme1390I CCNGG 3 cut(s) 85, 268, 269
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 2 cut(s) 86, 141
BmiI GGNNCC 2 cut(s) 187, 265
BmrFI CCNGG 3 cut(s) 85, 268, 269
BmsI GCATC 2 cut(s) 42, 226
BpuMI CCSGG 3 cut(s) 85, 268, 269
BsaBI GATNNNNATC 2 cut(s) 56, 62
BsaJI CCNNGG 3 cut(s) 24, 248, 267
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 182
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 176
Bse8I GATNNNNATC 2 cut(s) 56, 62
BseDI CCNNGG 3 cut(s) 24, 248, 267
BseGI GGATG 1 cut(s) 132
BseJI GATNNNNATC 2 cut(s) 56, 62
BseLI CCNNNNNNNGG 2 cut(s) 100, 182
BseMII CTCAG 2 cut(s) 233, 326
BseNI ACTGG 1 cut(s) 176
BseXI GCAGC 3 cut(s) 316, 322, 344
BsgI GTGCAG 1 cut(s) 199
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 88, 143
BsiEI CGRYCG 1 cut(s) 169
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 4 cut(s) 84, 94, 228, 268
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 2 cut(s) 100, 182
BsmAI GTCTC 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 367
BsnI GGCC 2 cut(s) 88, 143
BsoBI CYCGRG 1 cut(s) 267
Bsp143I GATC 6 cut(s) 57, 90, 190, 263, 360, 372
BspACI CCGC 1 cut(s) 225
BspANI GGCC 2 cut(s) 88, 143
BspCNI CTCAG 2 cut(s) 234, 325
BspLI GGNNCC 2 cut(s) 187, 265
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 4 cut(s) 85, 258, 271, 380
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 3 cut(s) 24, 248, 267
BssMI GATC 6 cut(s) 57, 90, 190, 263, 360, 372
BssT1I CCWWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 167
Bst6I CTCTTC 1 cut(s) 347
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 2 cut(s) 242, 312
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 1 cut(s) 132
BstKTI GATC 6 cut(s) 60, 93, 193, 266, 363, 375
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 6 cut(s) 57, 90, 190, 263, 360, 372
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 13, 335
BstSCI CCNGG 3 cut(s) 83, 266, 267
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 316, 322, 344
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 2 cut(s) 88, 143
BtgI CCRYGG 1 cut(s) 248
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 73, 231
Cac8I GCNNGC 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 86, 141
Cfr9I CCCGGG 1 cut(s) 267
Csp6I GTAC 1 cut(s) 81
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 2 cut(s) 242, 312
DpnI GATC 6 cut(s) 59, 92, 192, 265, 362, 374
DpnII GATC 6 cut(s) 57, 90, 190, 263, 360, 372
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Eco130I CCWWGG 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 141
EcoRI GAATTC 1 cut(s) 401
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaiI YATR 3 cut(s) 50, 211, 292
FaqI GGGAC 1 cut(s) 367
FbaI TGATCA 2 cut(s) 57, 360
Fnu4HI GCNGC 4 cut(s) 225, 330, 333, 336
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 4 cut(s) 225, 330, 333, 336
FspBI CTAG 1 cut(s) 119
GluI GCNGC 4 cut(s) 225, 330, 333, 336
HaeIII GGCC 2 cut(s) 88, 143
HapII CCGG 4 cut(s) 84, 94, 228, 268
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 3 cut(s) 63, 244, 310
HpaI GTTAAC 1 cut(s) 280
HpaII CCGG 4 cut(s) 84, 94, 228, 268
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 106, 280
Hpy188I TCNGA 3 cut(s) 62, 190, 243
Hpy8I GTNNAC 2 cut(s) 106, 280
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4V TGCA 3 cut(s) 216, 294, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 335
HpyF3I CTNAG 2 cut(s) 242, 312
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Ksp22I TGATCA 2 cut(s) 57, 360
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 6 cut(s) 57, 90, 190, 263, 360, 372
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 7 cut(s) 97, 107, 189, 241, 281, 327, 355
Lsp1109I GCAGC 3 cut(s) 316, 322, 344
LweI GCATC 2 cut(s) 42, 226
MaeI CTAG 1 cut(s) 119
MaeIII GTNAC 2 cut(s) 161, 344
MalI GATC 6 cut(s) 59, 92, 192, 265, 362, 374
MboI GATC 6 cut(s) 57, 90, 190, 263, 360, 372
MboII GAAGA 1 cut(s) 364
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 1 cut(s) 263
MluCI AATT 2 cut(s) 258, 401
MlyI GAGTC 2 cut(s) 253, 304
MnlI CCTC 3 cut(s) 265, 395, 415
MroNI GCCGGC 1 cut(s) 227
MseI TTAA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 4 cut(s) 84, 94, 228, 268
MspR9I CCNGG 3 cut(s) 85, 268, 269
MunI CAATTG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 13, 335
NaeI GCCGGC 1 cut(s) 229
NciI CCSGG 3 cut(s) 85, 268, 269
NdeII GATC 6 cut(s) 57, 90, 190, 263, 360, 372
NgoMIV GCCGGC 1 cut(s) 227
NlaIV GGNNCC 2 cut(s) 187, 265
NmuCI GTSAC 2 cut(s) 161, 344
PdiI GCCGGC 1 cut(s) 229
PfeI GAWTC 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 182
PkrI GCNGC 4 cut(s) 226, 331, 334, 337
PleI GAGTC 2 cut(s) 252, 304
PpsI GAGTC 2 cut(s) 252, 304
PspN4I GGNNCC 2 cut(s) 187, 265
PspPI GGNCC 2 cut(s) 86, 141
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 4 cut(s) 225, 330, 333, 336
Sau3AI GATC 6 cut(s) 57, 90, 190, 263, 360, 372
Sau96I GGNCC 2 cut(s) 86, 141
SchI GAGTC 2 cut(s) 253, 304
ScrFI CCNGG 3 cut(s) 85, 268, 269
SetI ASST 2 cut(s) 307, 337
SfaNI GCATC 2 cut(s) 42, 226
SfcI CTRYAG 1 cut(s) 330
SmaI CCCGGG 1 cut(s) 269
SpeI ACTAGT 1 cut(s) 118
Sse9I AATT 2 cut(s) 258, 401
SsiI CCGC 1 cut(s) 225
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 3 cut(s) 83, 266, 267
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 167
TasI AATT 2 cut(s) 258, 401
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 63
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 73, 238
TseFI GTSAC 2 cut(s) 161, 344
TseI GCWGC 3 cut(s) 329, 332, 335
Tsp45I GTSAC 2 cut(s) 161, 344
TspGWI ACGGA 1 cut(s) 237
TspMI CCCGGG 1 cut(s) 267
TspRI CASTG 2 cut(s) 73, 238
Van91I CCANNNNNTGG 1 cut(s) 182
XapI RAATTY 1 cut(s) 401
XmaI CCCGGG 1 cut(s) 267
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.