Rmu_sc0004744.1_g000002

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004744.1
OG0018058
Physical Location & Seq
Reverse (-)
2032 .. 2445
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004744.1_g000002.1.cds

Sequence Viewer

Length: 414 bp
atgttgctttacaagcagaagcagaagcccaaggaaaccaaaggcgacaatatgatgctgatcagcatcagtgtggtgggcagtactggcccgttccggttcgtcgtgaataagaaggaactagtcgctgcggtcctccaaagggccttgaagtcctacgcgcgcttaggtcggctaccagttcttggctccgatctcaaagagttcgtcctttactgctccactgccgccggcgtagatgctctgagtccttgggagacaattggatcccgaggggcagttaataacttcattctatgcaagaagccaactacgactcagccactaccattggctgcagcttctgataagaagaagagtctgatcaaggggtggatcaataagtccttcaatctcaacatctattcccattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.07

Weight (kDa)

10.01

Isoelectric Point (pI)

27.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26570
rosa_chinensis RchiOBHm_Chr2g0127581
rosa_laevigata RLG00000018953
rosa_multiflora Rmu_sc0004744.1_g000002
rosa_roxburghii Rroxscaffold_2G00116520
rosa_rugosa Rorug02G0273000
rosa_samantha Rh2BG334300 Rh2CG312300 Rh2CG312500 Rh2DG352400
rosa_wichuraiana Rw2G026380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 185
AccII CGCG 2 cut(s) 161, 163
AciI CCGC 2 cut(s) 131, 228
AclWI GGATC 3 cut(s) 261, 274, 383
AfaI GTAC 1 cut(s) 85
AfiI CCNNNNNNNGG 2 cut(s) 142, 185
AgsI TTSAA 2 cut(s) 151, 391
AhlI ACTAGT 1 cut(s) 121
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 3 cut(s) 261, 274, 383
AlwNI CAGNNNCTG 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 270
AoxI GGCC 2 cut(s) 88, 144
ApeKI GCWGC 3 cut(s) 128, 335, 338
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AspLEI GCGC 2 cut(s) 163, 165
AspS9I GGNCC 3 cut(s) 89, 133, 144
AvaI CYCGRG 1 cut(s) 270
AvaII GGWCC 1 cut(s) 133
BamHI GGATCC 1 cut(s) 266
BbvI GCAGC 3 cut(s) 115, 322, 350
BclI TGATCA 2 cut(s) 60, 363
BcoDI GTCTC 1 cut(s) 251
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 336
BisI GCNGC 4 cut(s) 129, 228, 336, 339
BlsI GCNGC 4 cut(s) 130, 229, 337, 340
BmcAI AGTACT 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 270
BmgT120I GGNCC 3 cut(s) 89, 133, 144
BmiI GGNNCC 2 cut(s) 190, 268
BmsI GCATC 3 cut(s) 45, 75, 229
Bpu10I CCTNAGC 1 cut(s) 166
BsaBI GATNNNNATC 2 cut(s) 59, 65
BsaJI CCNNGG 3 cut(s) 30, 251, 271
BsaWI WCCGGW 1 cut(s) 96
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 185
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 2 cut(s) 91, 179
Bse8I GATNNNNATC 2 cut(s) 59, 65
BseDI CCNNGG 3 cut(s) 30, 251, 271
BseJI GATNNNNATC 2 cut(s) 59, 65
BseLI CCNNNNNNNGG 2 cut(s) 142, 185
BseMII CTCAG 2 cut(s) 236, 332
BseNI ACTGG 2 cut(s) 91, 179
BsePI GCGCGC 1 cut(s) 161
BseXI GCAGC 3 cut(s) 115, 322, 350
Bsh1236I CGCG 2 cut(s) 161, 163
BshFI GGCC 2 cut(s) 90, 146
BsiHKCI CYCGRG 1 cut(s) 270
BsiSI CCGG 2 cut(s) 97, 231
BslI CCNNNNNNNGG 2 cut(s) 142, 185
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 2 cut(s) 90, 146
BsoBI CYCGRG 1 cut(s) 270
Bsp143I GATC 5 cut(s) 60, 193, 266, 363, 375
BspACI CCGC 2 cut(s) 131, 228
BspANI GGCC 2 cut(s) 90, 146
BspCNI CTCAG 2 cut(s) 237, 331
BspFNI CGCG 2 cut(s) 161, 163
BspLI GGNNCC 2 cut(s) 190, 268
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 3 cut(s) 261, 274, 383
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 2 cut(s) 91, 179
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 3 cut(s) 30, 251, 271
BssHII GCGCGC 1 cut(s) 161
BssMI GATC 5 cut(s) 60, 193, 266, 363, 375
BssT1I CCWWGG 2 cut(s) 30, 251
Bst6I CTCTTC 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 163, 232
BstDEI CTNAG 3 cut(s) 166, 245, 318
BstFNI CGCG 2 cut(s) 161, 163
BstHHI GCGC 2 cut(s) 163, 165
BstKTI GATC 5 cut(s) 63, 196, 269, 366, 378
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 5 cut(s) 60, 193, 266, 363, 375
BstMWI GCNNNNNNNGC 2 cut(s) 13, 87
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 2 cut(s) 161, 163
BstV1I GCAGC 3 cut(s) 115, 322, 350
BstX2I RGATCY 1 cut(s) 266
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 2 cut(s) 90, 146
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 76, 222
Cac8I GCNNGC 2 cut(s) 163, 232
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 2 cut(s) 163, 165
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 89, 133, 144
Csp6I GTAC 1 cut(s) 84
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviJI RGCY 9 cut(s) 28, 90, 146, 175, 189, 307, 322, 335, 341
CviKI_1 RGCY 9 cut(s) 28, 90, 146, 175, 189, 307, 322, 335, 341
CviQI GTAC 1 cut(s) 84
DdeI CTNAG 3 cut(s) 166, 245, 318
DpnI GATC 5 cut(s) 62, 195, 268, 365, 377
DpnII GATC 5 cut(s) 60, 193, 266, 363, 375
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Eco130I CCWWGG 2 cut(s) 30, 251
Eco47I GGWCC 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 30, 251
ErhI CCWWGG 2 cut(s) 30, 251
FaiI YATR 2 cut(s) 53, 298
FbaI TGATCA 2 cut(s) 60, 363
Fnu4HI GCNGC 4 cut(s) 129, 228, 336, 339
Fsp4HI GCNGC 4 cut(s) 129, 228, 336, 339
FspBI CTAG 1 cut(s) 122
GlaI GCGC 2 cut(s) 162, 164
GluI GCNGC 4 cut(s) 129, 228, 336, 339
HaeIII GGCC 2 cut(s) 90, 146
HapII CCGG 2 cut(s) 97, 231
HhaI GCGC 2 cut(s) 163, 165
Hin6I GCGC 2 cut(s) 161, 163
HinP1I GCGC 2 cut(s) 161, 163
HinfI GANTC 3 cut(s) 247, 316, 358
HpaII CCGG 2 cut(s) 97, 231
Hpy188I TCNGA 4 cut(s) 193, 246, 346, 363
Hpy188III TCNNGA 2 cut(s) 106, 270
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 2 cut(s) 109, 397
HpyCH4V TGCA 2 cut(s) 300, 338
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 87
HpyF3I CTNAG 3 cut(s) 166, 245, 318
HspAI GCGC 2 cut(s) 161, 163
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Ksp22I TGATCA 2 cut(s) 60, 363
Kzo9I GATC 5 cut(s) 60, 193, 266, 363, 375
LmnI GCTCC 2 cut(s) 194, 224
LpnPI CCDG 4 cut(s) 72, 110, 192, 244
Lsp1109I GCAGC 3 cut(s) 115, 322, 350
LweI GCATC 3 cut(s) 45, 75, 229
MaeI CTAG 1 cut(s) 122
MalI GATC 5 cut(s) 62, 195, 268, 365, 377
MboI GATC 5 cut(s) 60, 193, 266, 363, 375
MboII GAAGA 2 cut(s) 364, 367
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 1 cut(s) 266
MluCI AATT 1 cut(s) 261
MlyI GAGTC 3 cut(s) 256, 310, 367
MnlI CCTC 2 cut(s) 146, 266
MreI CGCCGGCG 1 cut(s) 230
MroNI GCCGGC 1 cut(s) 230
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 71
MspI CCGG 2 cut(s) 97, 231
MunI CAATTG 1 cut(s) 261
MvnI CGCG 2 cut(s) 161, 163
MwoI GCNNNNNNNGC 2 cut(s) 13, 87
NaeI GCCGGC 1 cut(s) 232
NdeII GATC 5 cut(s) 60, 193, 266, 363, 375
NgoMIV GCCGGC 1 cut(s) 230
NlaIV GGNNCC 2 cut(s) 190, 268
PauI GCGCGC 1 cut(s) 161
PdiI GCCGGC 1 cut(s) 232
PflMI CCANNNNNTGG 1 cut(s) 185
PkrI GCNGC 4 cut(s) 130, 229, 337, 340
PleI GAGTC 3 cut(s) 255, 310, 366
PpsI GAGTC 3 cut(s) 255, 310, 366
PspN4I GGNNCC 2 cut(s) 190, 268
PspPI GGNCC 3 cut(s) 89, 133, 144
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 266
PteI GCGCGC 1 cut(s) 161
RsaI GTAC 1 cut(s) 85
RsaNI GTAC 1 cut(s) 84
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 4 cut(s) 129, 228, 336, 339
Sau3AI GATC 5 cut(s) 60, 193, 266, 363, 375
Sau96I GGNCC 3 cut(s) 89, 133, 144
ScaI AGTACT 1 cut(s) 85
SchI GAGTC 3 cut(s) 256, 310, 367
SetI ASST 2 cut(s) 172, 343
SfaNI GCATC 3 cut(s) 45, 75, 229
SfcI CTRYAG 1 cut(s) 336
SgrAI CRCCGGYG 1 cut(s) 230
SinI GGWCC 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 121
Sse9I AATT 1 cut(s) 261
SsiI CCGC 2 cut(s) 131, 228
SspMI CTAG 1 cut(s) 122
StyI CCWWGG 2 cut(s) 30, 251
TasI AATT 1 cut(s) 261
TatI WGTACW 1 cut(s) 83
TauI GCSGC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 2 cut(s) 76, 229
TseI GCWGC 3 cut(s) 128, 335, 338
TspDTI ATGAA 1 cut(s) 280
TspRI CASTG 2 cut(s) 76, 229
Van91I CCANNNNNTGG 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 133
XspI CTAG 1 cut(s) 122
ZrmI AGTACT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.