Rw2G026380

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018058
Physical Location & Seq
Reverse (-)
40636331 .. 40636738
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G026380.1

Sequence Viewer

Length: 375 bp
ATGTTGCTTTACAAGCAGAAGCCCAAGGAAACCAAAGGCGACAATATGATGCTGATCAGCATCAGTGTGGTGGGCAGTACTGGCCCGTTCCGGTTCGTCGTGAATAAGAAGGAACTAGTCGCTGCGGTCCTCCAAAGGGCCTTGAAGTCCTACGCGCGCTTAGGTCGGCTACCAGTTCTTGGCTCCGATCTCAAAGAGTTCGTCCTTTACTGCTCCACTGCCGCCGGCGTAGATGCTCTGATTAATAACTTCATTCTATGCAAGAAGCCACCTACGACTCAACCACTACCATTGGCTGCAGCTTCTGATCAGAAGAAGAGTCTGATCAAGGGGTGGATCAATAAGTCCTTCAATCTCAACATCTATTCCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

13.68

Weight (kDa)

9.92

Isoelectric Point (pI)

26.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 15 - 74 5.8e-15 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26570
rosa_chinensis RchiOBHm_Chr2g0127581
rosa_laevigata RLG00000018953
rosa_multiflora Rmu_sc0004744.1_g000002
rosa_roxburghii Rroxscaffold_2G00116520
rosa_rugosa Rorug02G0273000
rosa_samantha Rh2BG334300 Rh2CG312300 Rh2CG312500 Rh2DG352400
rosa_wichuraiana Rw2G026380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 179
AccII CGCG 2 cut(s) 155, 157
AciI CCGC 2 cut(s) 125, 222
AclWI GGATC 1 cut(s) 344
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 2 cut(s) 136, 179
AgsI TTSAA 2 cut(s) 145, 352
AhlI ACTAGT 1 cut(s) 115
AluBI AGCT 1 cut(s) 302
AluI AGCT 1 cut(s) 302
AlwI GGATC 1 cut(s) 344
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 2 cut(s) 82, 138
ApeKI GCWGC 3 cut(s) 122, 296, 299
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AseI ATTAAT 1 cut(s) 243
AspLEI GCGC 2 cut(s) 157, 159
AspS9I GGNCC 3 cut(s) 83, 127, 138
AvaII GGWCC 1 cut(s) 127
BbvI GCAGC 3 cut(s) 109, 283, 311
BclI TGATCA 3 cut(s) 54, 307, 324
BcuI ACTAGT 1 cut(s) 115
BfaI CTAG 1 cut(s) 116
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 4 cut(s) 123, 222, 297, 300
BlsI GCNGC 4 cut(s) 124, 223, 298, 301
BmcAI AGTACT 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 83, 127, 138
BmiI GGNNCC 1 cut(s) 184
BmsI GCATC 3 cut(s) 39, 69, 223
Bpu10I CCTNAGC 1 cut(s) 160
BsaBI GATNNNNATC 2 cut(s) 53, 59
BsaJI CCNNGG 1 cut(s) 24
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 179
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 85, 173
Bse8I GATNNNNATC 2 cut(s) 53, 59
BseDI CCNNGG 1 cut(s) 24
BseJI GATNNNNATC 2 cut(s) 53, 59
BseLI CCNNNNNNNGG 2 cut(s) 136, 179
BseNI ACTGG 2 cut(s) 85, 173
BsePI GCGCGC 1 cut(s) 155
BseXI GCAGC 3 cut(s) 109, 283, 311
Bsh1236I CGCG 2 cut(s) 155, 157
BshFI GGCC 2 cut(s) 84, 140
BsiSI CCGG 2 cut(s) 91, 225
BslI CCNNNNNNNGG 2 cut(s) 136, 179
BsnI GGCC 2 cut(s) 84, 140
Bsp143I GATC 5 cut(s) 54, 187, 307, 324, 336
BspACI CCGC 2 cut(s) 125, 222
BspANI GGCC 2 cut(s) 84, 140
BspFNI CGCG 2 cut(s) 155, 157
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 2 cut(s) 85, 173
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 24
BssHII GCGCGC 1 cut(s) 155
BssMI GATC 5 cut(s) 54, 187, 307, 324, 336
BssT1I CCWWGG 1 cut(s) 24
Bst6I CTCTTC 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 157, 226
BstDEI CTNAG 1 cut(s) 160
BstFNI CGCG 2 cut(s) 155, 157
BstHHI GCGC 2 cut(s) 157, 159
BstKTI GATC 5 cut(s) 57, 190, 310, 327, 339
BstMBI GATC 5 cut(s) 54, 187, 307, 324, 336
BstMWI GCNNNNNNNGC 2 cut(s) 13, 81
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 2 cut(s) 155, 157
BstV1I GCAGC 3 cut(s) 109, 283, 311
BsuRI GGCC 2 cut(s) 84, 140
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 70, 216
Cac8I GCNNGC 2 cut(s) 157, 226
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 2 cut(s) 157, 159
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 83, 127, 138
Csp6I GTAC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviJI RGCY 8 cut(s) 22, 84, 140, 169, 183, 268, 296, 302
CviKI_1 RGCY 8 cut(s) 22, 84, 140, 169, 183, 268, 296, 302
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 1 cut(s) 160
DpnI GATC 5 cut(s) 56, 189, 309, 326, 338
DpnII GATC 5 cut(s) 54, 187, 307, 324, 336
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaiI YATR 2 cut(s) 47, 259
FbaI TGATCA 3 cut(s) 54, 307, 324
Fnu4HI GCNGC 4 cut(s) 123, 222, 297, 300
Fsp4HI GCNGC 4 cut(s) 123, 222, 297, 300
FspBI CTAG 1 cut(s) 116
GlaI GCGC 2 cut(s) 156, 158
GluI GCNGC 4 cut(s) 123, 222, 297, 300
HaeIII GGCC 2 cut(s) 84, 140
HapII CCGG 2 cut(s) 91, 225
HhaI GCGC 2 cut(s) 157, 159
Hin6I GCGC 2 cut(s) 155, 157
HinP1I GCGC 2 cut(s) 155, 157
HinfI GANTC 2 cut(s) 277, 319
HpaII CCGG 2 cut(s) 91, 225
Hpy188I TCNGA 5 cut(s) 187, 240, 307, 312, 324
Hpy188III TCNNGA 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 103, 358
HpyCH4V TGCA 2 cut(s) 261, 299
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 81
HpyF3I CTNAG 1 cut(s) 160
HspAI GCGC 2 cut(s) 155, 157
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 3 cut(s) 54, 307, 324
Kzo9I GATC 5 cut(s) 54, 187, 307, 324, 336
LmnI GCTCC 2 cut(s) 188, 218
LpnPI CCDG 4 cut(s) 66, 104, 186, 238
Lsp1109I GCAGC 3 cut(s) 109, 283, 311
LweI GCATC 3 cut(s) 39, 69, 223
MaeI CTAG 1 cut(s) 116
MalI GATC 5 cut(s) 56, 189, 309, 326, 338
MboI GATC 5 cut(s) 54, 187, 307, 324, 336
MboII GAAGA 2 cut(s) 325, 328
MlyI GAGTC 2 cut(s) 271, 328
MnlI CCTC 1 cut(s) 140
MreI CGCCGGCG 1 cut(s) 224
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 65
MspI CCGG 2 cut(s) 91, 225
MvnI CGCG 2 cut(s) 155, 157
MwoI GCNNNNNNNGC 2 cut(s) 13, 81
NaeI GCCGGC 1 cut(s) 226
NdeII GATC 5 cut(s) 54, 187, 307, 324, 336
NgoMIV GCCGGC 1 cut(s) 224
NlaIV GGNNCC 1 cut(s) 184
PauI GCGCGC 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 179
PkrI GCNGC 4 cut(s) 124, 223, 298, 301
PleI GAGTC 2 cut(s) 271, 327
PpsI GAGTC 2 cut(s) 271, 327
PshBI ATTAAT 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 3 cut(s) 83, 127, 138
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 305
PteI GCGCGC 1 cut(s) 155
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 4 cut(s) 123, 222, 297, 300
Sau3AI GATC 5 cut(s) 54, 187, 307, 324, 336
Sau96I GGNCC 3 cut(s) 83, 127, 138
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 2 cut(s) 271, 328
SetI ASST 3 cut(s) 166, 274, 304
SfaNI GCATC 3 cut(s) 39, 69, 223
SfcI CTRYAG 1 cut(s) 297
SgrAI CRCCGGYG 1 cut(s) 224
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 65
SpeI ACTAGT 1 cut(s) 115
SsiI CCGC 2 cut(s) 125, 222
SspMI CTAG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 24
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 2 cut(s) 70, 223
TseI GCWGC 3 cut(s) 122, 296, 299
TspDTI ATGAA 1 cut(s) 241
TspRI CASTG 2 cut(s) 70, 223
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 127
VspI ATTAAT 1 cut(s) 243
XspI CTAG 1 cut(s) 116
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.