Rh2BG334300

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018058
Physical Location & Seq
Reverse (-)
44152651 .. 44153671
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG334300.1

Sequence Viewer

Length: 408 bp
ATGTTGCTTTACAAGCAGAAGCCCAAGGAAACCAAAGGCGACAATATGATGCTGATCAGCATCAGTGTGGTGGGCAGTACTGGCCCGTTCCGGTTCGTCGTGAATAAGAAGGAACTAGTCGCTGCGGTCCTCCAAAGGGCCTTGAAGTCCTACGCGCGCTTAGGTCGGCTACCAGTTCTTGGCTCCGATCTCAAAGAGTTCGTCCTTTACTGCTCCACTGCCGCCGGCGTAGATGCTCTGAGTCCTTGGGAGACAATTGGATCCCGAGGGGCAGTTAATAACTTCATTCTATGCAAGAAGCCACCTACGACTCAGCCACTACCATTGGCTGCAGCTTCTGATAAGAAGAAGAGTCTGATCAAGGGGTGGATCAATAAGTCCTTCAATCTCAACATCTATTCCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.81

Weight (kDa)

9.97

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 15 - 98 1.3e-19 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26570
rosa_chinensis RchiOBHm_Chr2g0127581
rosa_laevigata RLG00000018953
rosa_multiflora Rmu_sc0004744.1_g000002
rosa_roxburghii Rroxscaffold_2G00116520
rosa_rugosa Rorug02G0273000
rosa_samantha Rh2BG334300 Rh2CG312300 Rh2CG312500 Rh2DG352400
rosa_wichuraiana Rw2G026380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 179
AccII CGCG 2 cut(s) 155, 157
AciI CCGC 2 cut(s) 125, 222
AclWI GGATC 3 cut(s) 255, 268, 377
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 2 cut(s) 136, 179
AgsI TTSAA 2 cut(s) 145, 385
AhlI ACTAGT 1 cut(s) 115
AluBI AGCT 1 cut(s) 335
AluI AGCT 1 cut(s) 335
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 3 cut(s) 255, 268, 377
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 2 cut(s) 82, 138
ApeKI GCWGC 3 cut(s) 122, 329, 332
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AspLEI GCGC 2 cut(s) 157, 159
AspS9I GGNCC 3 cut(s) 83, 127, 138
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 1 cut(s) 127
BamHI GGATCC 1 cut(s) 260
BbvI GCAGC 3 cut(s) 109, 316, 344
BclI TGATCA 2 cut(s) 54, 357
BcoDI GTCTC 1 cut(s) 245
BcuI ACTAGT 1 cut(s) 115
BfaI CTAG 1 cut(s) 116
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 4 cut(s) 123, 222, 330, 333
BlsI GCNGC 4 cut(s) 124, 223, 331, 334
BmcAI AGTACT 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 3 cut(s) 83, 127, 138
BmiI GGNNCC 2 cut(s) 184, 262
BmsI GCATC 3 cut(s) 39, 69, 223
Bpu10I CCTNAGC 1 cut(s) 160
BsaBI GATNNNNATC 2 cut(s) 53, 59
BsaJI CCNNGG 3 cut(s) 24, 245, 265
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 179
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 85, 173
Bse8I GATNNNNATC 2 cut(s) 53, 59
BseDI CCNNGG 3 cut(s) 24, 245, 265
BseJI GATNNNNATC 2 cut(s) 53, 59
BseLI CCNNNNNNNGG 2 cut(s) 136, 179
BseMII CTCAG 2 cut(s) 230, 326
BseNI ACTGG 2 cut(s) 85, 173
BsePI GCGCGC 1 cut(s) 155
BseXI GCAGC 3 cut(s) 109, 316, 344
Bsh1236I CGCG 2 cut(s) 155, 157
BshFI GGCC 2 cut(s) 84, 140
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 2 cut(s) 91, 225
BslI CCNNNNNNNGG 2 cut(s) 136, 179
BsmAI GTCTC 1 cut(s) 245
BsnI GGCC 2 cut(s) 84, 140
BsoBI CYCGRG 1 cut(s) 264
Bsp143I GATC 5 cut(s) 54, 187, 260, 357, 369
BspACI CCGC 2 cut(s) 125, 222
BspANI GGCC 2 cut(s) 84, 140
BspCNI CTCAG 2 cut(s) 231, 325
BspFNI CGCG 2 cut(s) 155, 157
BspLI GGNNCC 2 cut(s) 184, 262
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 3 cut(s) 255, 268, 377
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 2 cut(s) 85, 173
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 3 cut(s) 24, 245, 265
BssHII GCGCGC 1 cut(s) 155
BssMI GATC 5 cut(s) 54, 187, 260, 357, 369
BssT1I CCWWGG 2 cut(s) 24, 245
Bst6I CTCTTC 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 157, 226
BstDEI CTNAG 3 cut(s) 160, 239, 312
BstFNI CGCG 2 cut(s) 155, 157
BstHHI GCGC 2 cut(s) 157, 159
BstKTI GATC 5 cut(s) 57, 190, 263, 360, 372
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 5 cut(s) 54, 187, 260, 357, 369
BstMWI GCNNNNNNNGC 2 cut(s) 13, 81
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 2 cut(s) 155, 157
BstV1I GCAGC 3 cut(s) 109, 316, 344
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
BsuRI GGCC 2 cut(s) 84, 140
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 70, 216
Cac8I GCNNGC 2 cut(s) 157, 226
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 2 cut(s) 157, 159
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 83, 127, 138
Csp6I GTAC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 306, 341
CviJI RGCY 9 cut(s) 22, 84, 140, 169, 183, 301, 316, 329, 335
CviKI_1 RGCY 9 cut(s) 22, 84, 140, 169, 183, 301, 316, 329, 335
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 3 cut(s) 160, 239, 312
DpnI GATC 5 cut(s) 56, 189, 262, 359, 371
DpnII GATC 5 cut(s) 54, 187, 260, 357, 369
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 24, 245
Eco47I GGWCC 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 24, 245
ErhI CCWWGG 2 cut(s) 24, 245
FaiI YATR 2 cut(s) 47, 292
FbaI TGATCA 2 cut(s) 54, 357
Fnu4HI GCNGC 4 cut(s) 123, 222, 330, 333
Fsp4HI GCNGC 4 cut(s) 123, 222, 330, 333
FspBI CTAG 1 cut(s) 116
GlaI GCGC 2 cut(s) 156, 158
GluI GCNGC 4 cut(s) 123, 222, 330, 333
HaeIII GGCC 2 cut(s) 84, 140
HapII CCGG 2 cut(s) 91, 225
HhaI GCGC 2 cut(s) 157, 159
Hin6I GCGC 2 cut(s) 155, 157
HinP1I GCGC 2 cut(s) 155, 157
HinfI GANTC 3 cut(s) 241, 310, 352
HpaII CCGG 2 cut(s) 91, 225
Hpy188I TCNGA 4 cut(s) 187, 240, 340, 357
Hpy188III TCNNGA 2 cut(s) 100, 264
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 103, 391
HpyCH4V TGCA 2 cut(s) 294, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 81
HpyF3I CTNAG 3 cut(s) 160, 239, 312
HspAI GCGC 2 cut(s) 155, 157
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 2 cut(s) 54, 357
Kzo9I GATC 5 cut(s) 54, 187, 260, 357, 369
LmnI GCTCC 2 cut(s) 188, 218
LpnPI CCDG 4 cut(s) 66, 104, 186, 238
Lsp1109I GCAGC 3 cut(s) 109, 316, 344
LweI GCATC 3 cut(s) 39, 69, 223
MaeI CTAG 1 cut(s) 116
MalI GATC 5 cut(s) 56, 189, 262, 359, 371
MboI GATC 5 cut(s) 54, 187, 260, 357, 369
MboII GAAGA 2 cut(s) 358, 361
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 260
MluCI AATT 1 cut(s) 255
MlyI GAGTC 3 cut(s) 250, 304, 361
MnlI CCTC 2 cut(s) 140, 260
MreI CGCCGGCG 1 cut(s) 224
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 65
MspI CCGG 2 cut(s) 91, 225
MunI CAATTG 1 cut(s) 255
MvnI CGCG 2 cut(s) 155, 157
MwoI GCNNNNNNNGC 2 cut(s) 13, 81
NaeI GCCGGC 1 cut(s) 226
NdeII GATC 5 cut(s) 54, 187, 260, 357, 369
NgoMIV GCCGGC 1 cut(s) 224
NlaIV GGNNCC 2 cut(s) 184, 262
PauI GCGCGC 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 179
PkrI GCNGC 4 cut(s) 124, 223, 331, 334
PleI GAGTC 3 cut(s) 249, 304, 360
PpsI GAGTC 3 cut(s) 249, 304, 360
PspN4I GGNNCC 2 cut(s) 184, 262
PspPI GGNCC 3 cut(s) 83, 127, 138
PstI CTGCAG 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 1 cut(s) 260
PteI GCGCGC 1 cut(s) 155
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 4 cut(s) 123, 222, 330, 333
Sau3AI GATC 5 cut(s) 54, 187, 260, 357, 369
Sau96I GGNCC 3 cut(s) 83, 127, 138
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 3 cut(s) 250, 304, 361
SetI ASST 3 cut(s) 166, 307, 337
SfaNI GCATC 3 cut(s) 39, 69, 223
SfcI CTRYAG 1 cut(s) 330
SgrAI CRCCGGYG 1 cut(s) 224
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 65
SpeI ACTAGT 1 cut(s) 115
Sse9I AATT 1 cut(s) 255
SsiI CCGC 2 cut(s) 125, 222
SspMI CTAG 1 cut(s) 116
StyI CCWWGG 2 cut(s) 24, 245
TasI AATT 1 cut(s) 255
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 2 cut(s) 70, 223
TseI GCWGC 3 cut(s) 122, 329, 332
TspDTI ATGAA 1 cut(s) 274
TspRI CASTG 2 cut(s) 70, 223
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 127
XspI CTAG 1 cut(s) 116
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.