FvH4_6g42450

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009531
Physical Location & Seq
Forward (+)
33094703 .. 33097632
2930 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42450.t4

Sequence Viewer

Length: 1770 bp
ATGGTTCTGGTGTCGAGCAACAGTGAGAAAGATGGTGCAGCAAATGATGGGAGTATGGTGGATTTTCGAGGAAACCCTATCGACAAGTCCAGAACTGGTGGATGGCTTGCTGCAGGGCTTATCTTAGTAACTGAGCTCTCTGAGAGGATCTGTGTGATGGGCATATCTATGAATTTAGTGACTTACTTGGTTGGCAATTTGCATTTAACTTCAGCAAAGTCTGCAACCGTAGTCACCAATTTCATGGGCACTTTGAATCTCCTTGGCCTTCTTGGTGGTTTCGTTGCAGATGCTAAACTCGGACGCTACCTGACTGTACTACTCTCTGCAGCCATAACTGCTCTGGGGATAACTTTGTTAACAATCGCCACATCCATTCCGAGTATGAGACCCCCTGCGTGCAAAGGCAACCAAGAATGCGTCGAGGCCACAGGCAGCCAATTAGCCCTGCTCTATGCGGCTTTATACATGACAGCACTAGGTGGTGGTGGAATAAAATCCAACGTCTCAGGTTTTGGCTCTGACCAATTTGATTCCTCAGACCCAAAGGAAGAGAAGTCCATGATCTTTTTCTTCAACAGGTTCTACTTTGGCATCAGCGTCGGTTCTCTATTTGCTGTGATTGTGCTGGTGTATATACAAGACAATATTGGCCGAGGATTGGGTTATGGAATATCAGCAGGGACAGTGGTGATCGCACTCATTGTGTTACTCTTGGGAACGCCATTCTATCGATACAAGAAGCCTCGAGGCAGCCCTTTAACAATGATCTGGAGGGTGATATTTTTAGCATGGAAGAAGAGGACTCATCCTTATCCTTCTCATCCAAGCCTTCTGAATCAATACGAAGAAGCCAAGATTCCACACACACAGAGATTCAAGTGCCTCGACAAAGCTGCAATACTAGATGAATTAGCTGCTAGTGGTGAAAATAGAAGCAACCCTTGGGTAGTATCCACAGTGACTCAAGTTGAAGAGGTGAAACTGGTTTTCAAGCTCTTGCCAATCTGGTCAACATGTATTCTCTTTTGGACGGTCTATTCTCAAATGACTACCTTCACCATTGAAGAAGCCACCTTCATGAACCGGAAAGTAGGTTCCTTTGAAATCCCTGCTGGTTCCTTCTCAGCCTTTCTGTTCATCACCATTCTCCTCTTCACCTCCCTAAATGAGAAACTTTTCGTCCCCCTTGCTCGAAAACTCACAAAGAGTCCCCAAGGTCTCACAAGCCTTCAGAGAGTTGGAATTGGACTCGTCTTTTCAACGGTTGCTATGATCGCTGCCGCAATAGTAGAGAAACAGAGAAGGGAAATGGCTGTTGACCACAACACAAAAATACGTGCCTTTTGGCTTGTTCCACAATACTTCCTAGTGGGTGCTGGGGAAGCTTTTGTATATGTAGGACAACTAGAATTTTTCATCAGAGAGGCACCGGACAGGATGAAGTCTATGAGCACAGGGCTCTTCTTAAGCACCATCTCAATGGGGTTCTACGTAAGCAGTCTGTTGGTGACCGTGGTGGATAAACTGACTCATAAGAGCTGGCTACGAAGCGATTTGAACAATGGAAGGCTAGAGAACTTCTATTTGCTGCTTGCAGCTCTAGGAACAATAAATTTCTTAGTTTTCCTTGCCTTTGCTACTAGACACCAATACAAAGGCCAGCAGTACAATGATCCTGATGACAGTGGCAAAGAGATGAAGAACTCTAATGACATGATACTAGAGGATATTGAGAAGATCAGAATTGAAGCAAAAGAGGGACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

590

Amino Acids

64.93

Weight (kDa)

9.0

Isoelectric Point (pI)

26.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 18 - 384 5.6e-100 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 747
AccB1I GGYRCC 1 cut(s) 1429
AciI CCGC 2 cut(s) 458, 1284
AclWI GGATC 2 cut(s) 155, 1670
AcoI YGGCCR 1 cut(s) 652
AcsI RAATTY 3 cut(s) 172, 1412, 1615
AcuI CTGAAG 2 cut(s) 195, 1217
AdeI CACNNNGTG 1 cut(s) 482
AfaI GTAC 2 cut(s) 318, 1670
AfiI CCNNNNNNNGG 1 cut(s) 661
AflII CTTAAG 1 cut(s) 1468
AflIII ACRYGT 1 cut(s) 1016
AjuI GAANNNNNNNTTGG 2 cut(s) 928, 960
AloI GAACNNNNNNTCC 2 cut(s) 1081, 1113
AluBI AGCT 7 cut(s) 136, 896, 917, 997, 1388, 1542, 1601
AluI AGCT 7 cut(s) 136, 896, 917, 997, 1388, 1542, 1601
Alw21I GWGCWC 2 cut(s) 138, 1457
Alw26I GTCTC 3 cut(s) 382, 511, 1226
AlwI GGATC 2 cut(s) 155, 1670
Ama87I CYCGRG 1 cut(s) 747
AoxI GGCC 4 cut(s) 265, 426, 652, 1660
ApoI RAATTY 3 cut(s) 172, 1412, 1615
Asp700I GAANNNNTTC 1 cut(s) 1178
AspS9I GGNCC 1 cut(s) 1763
AsuHPI GGTGA 9 cut(s) 226, 703, 790, 938, 991, 1051, 1135, 1150, 1522
AvaI CYCGRG 1 cut(s) 747
AvaII GGWCC 1 cut(s) 1763
BaeGI GKGCMC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 1429
BanII GRGCYC 2 cut(s) 138, 1464
Bbv12I GWGCWC 2 cut(s) 138, 1457
BccI CCATC 5 cut(s) 26, 41, 96, 151, 1484
BcgI CGANNNNNNTGC 4 cut(s) 583, 617, 878, 912
BciVI GTATCC 1 cut(s) 964
BcoDI GTCTC 3 cut(s) 382, 511, 1226
BfmI CTRYAG 2 cut(s) 111, 327
BfrI CTTAAG 1 cut(s) 1468
BfuI GTATCC 1 cut(s) 964
Bme18I GGWCC 1 cut(s) 1763
BmeT110I CYCGRG 1 cut(s) 747
BmgT120I GGNCC 1 cut(s) 1763
BmiI GGNNCC 5 cut(s) 1099, 1120, 1431, 1764, 1765
BmsI GCATC 2 cut(s) 280, 603
BpmI CTGGAG 1 cut(s) 793
BpuEI CTTGAG 1 cut(s) 951
Bsa29I ATCGAT 1 cut(s) 733
BsaAI YACGTR 2 cut(s) 1340, 1495
BsaI GGTCTC 2 cut(s) 382, 1226
BsaJI CCNNGG 5 cut(s) 262, 655, 944, 1216, 1515
BsaWI WCCGGW 2 cut(s) 1086, 1432
Bsc4I CCNNNNNNNGG 1 cut(s) 661
Bse1I ACTGG 2 cut(s) 100, 990
BseCI ATCGAT 1 cut(s) 733
BseDI CCNNGG 5 cut(s) 262, 655, 944, 1216, 1515
BseGI GGATG 5 cut(s) 107, 371, 808, 823, 1446
BseLI CCNNNNNNNGG 1 cut(s) 661
BseMII CTCAG 5 cut(s) 123, 132, 522, 552, 1140
BseNI ACTGG 2 cut(s) 100, 990
BseRI GAGGAG 1 cut(s) 1142
BseSI GKGCMC 1 cut(s) 251
BseYI CCCAGC 1 cut(s) 1379
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 4 cut(s) 267, 428, 654, 1662
BshNI GGYRCC 1 cut(s) 1429
BshVI ATCGAT 1 cut(s) 733
BsiHKAI GWGCWC 2 cut(s) 138, 1457
BsiHKCI CYCGRG 1 cut(s) 747
BsiSI CCGG 2 cut(s) 1087, 1433
BslFI GGGAC 3 cut(s) 697, 1169, 1197
BslI CCNNNNNNNGG 1 cut(s) 661
BsmAI GTCTC 3 cut(s) 382, 511, 1226
BsmBI CGTCTC 1 cut(s) 511
BsmFI GGGAC 3 cut(s) 697, 1169, 1197
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 4 cut(s) 267, 428, 654, 1662
Bso31I GGTCTC 2 cut(s) 382, 1226
BsoBI CYCGRG 1 cut(s) 747
Bsp1286I GDGCHC 4 cut(s) 138, 251, 1457, 1464
Bsp143I GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
BspACI CCGC 2 cut(s) 458, 1284
BspANI GGCC 4 cut(s) 267, 428, 654, 1662
BspCNI CTCAG 5 cut(s) 124, 133, 521, 551, 1139
BspDI ATCGAT 1 cut(s) 733
BspHI TCATGA 1 cut(s) 1080
BspLI GGNNCC 5 cut(s) 1099, 1120, 1431, 1764, 1765
BspMAI CTGCAG 2 cut(s) 115, 331
BspPI GGATC 2 cut(s) 155, 1670
BspQI GCTCTTC 1 cut(s) 1469
BspT107I GGYRCC 1 cut(s) 1429
BspTI CTTAAG 1 cut(s) 1468
BspTNI GGTCTC 2 cut(s) 382, 1226
BsrI ACTGG 2 cut(s) 100, 990
BssECI CCNNGG 5 cut(s) 262, 655, 944, 1216, 1515
BssMI GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
BssT1I CCWWGG 3 cut(s) 262, 944, 1216
Bst4CI ACNGT 9 cut(s) 23, 229, 316, 688, 961, 1036, 1267, 1516, 1688
Bst6I CTCTTC 5 cut(s) 546, 794, 969, 1160, 1469
BstAFI CTTAAG 1 cut(s) 1468
BstAPI GCANNNNNTGC 1 cut(s) 221
BstBAI YACGTR 2 cut(s) 1340, 1495
BstC8I GCNNGC 5 cut(s) 108, 400, 1544, 1596, 1664
BstDEI CTNAG 7 cut(s) 124, 132, 141, 508, 538, 1126, 1621
BstDSI CCRYGG 1 cut(s) 1515
BstEII GGTNACC 1 cut(s) 1510
BstF5I GGATG 5 cut(s) 107, 371, 808, 823, 1446
BstKTI GATC 7 cut(s) 150, 567, 696, 771, 1278, 1678, 1743
BstMAI GTCTC 3 cut(s) 382, 511, 1226
BstMBI GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
BstMWI GCNNNNNNNGC 4 cut(s) 221, 338, 1277, 1385
BstNSI RCATGY 1 cut(s) 1020
BstPI GGTNACC 1 cut(s) 1510
BstSFI CTRYAG 2 cut(s) 111, 327
BstSLI GKGCMC 1 cut(s) 251
BstSNI TACGTA 1 cut(s) 1495
BstX2I RGATCY 1 cut(s) 147
BstXI CCANNNNNNTGG 2 cut(s) 244, 1483
BstYI RGATCY 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 733
BsuI GTATCC 1 cut(s) 964
BsuRI GGCC 4 cut(s) 267, 428, 654, 1662
BsuTUI ATCGAT 1 cut(s) 733
BtgI CCRYGG 1 cut(s) 1515
BtsCI GGATG 5 cut(s) 107, 371, 808, 823, 1446
BtsIMutI CAGTG 4 cut(s) 28, 693, 966, 1693
Cac8I GCNNGC 5 cut(s) 108, 400, 1544, 1596, 1664
CciI TCATGA 1 cut(s) 1080
Cfr13I GGNCC 1 cut(s) 1763
ClaI ATCGAT 1 cut(s) 733
CseI GACGC 3 cut(s) 312, 409, 589
Csp6I GTAC 2 cut(s) 317, 1669
CviAII CATG 7 cut(s) 244, 469, 562, 792, 1017, 1081, 1717
CviQI GTAC 2 cut(s) 317, 1669
DdeI CTNAG 7 cut(s) 124, 132, 141, 508, 538, 1126, 1621
DpnI GATC 7 cut(s) 149, 566, 695, 770, 1277, 1677, 1742
DpnII GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
DraIII CACNNNGTG 1 cut(s) 482
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 5 cut(s) 546, 794, 969, 1160, 1469
EarI CTCTTC 5 cut(s) 546, 794, 969, 1160, 1469
Ecl136II GAGCTC 1 cut(s) 136
Eco105I TACGTA 1 cut(s) 1495
Eco130I CCWWGG 3 cut(s) 262, 944, 1216
Eco24I GRGCYC 2 cut(s) 138, 1464
Eco31I GGTCTC 2 cut(s) 382, 1226
Eco47I GGWCC 1 cut(s) 1763
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 195, 1217
Eco88I CYCGRG 1 cut(s) 747
Eco91I GGTNACC 1 cut(s) 1510
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 1763
EcoO65I GGTNACC 1 cut(s) 1510
EcoT14I CCWWGG 3 cut(s) 262, 944, 1216
EcoT38I GRGCYC 2 cut(s) 138, 1464
ErhI CCWWGG 3 cut(s) 262, 944, 1216
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 7 cut(s) 247, 472, 565, 795, 1020, 1084, 1720
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 3 cut(s) 697, 1169, 1197
FatI CATG 7 cut(s) 243, 468, 561, 791, 1016, 1080, 1716
FokI GGATG 5 cut(s) 114, 358, 795, 810, 1453
FriOI GRGCYC 2 cut(s) 138, 1464
GsaI CCCAGC 1 cut(s) 1383
GsuI CTGGAG 1 cut(s) 793
HaeIII GGCC 4 cut(s) 267, 428, 654, 1662
HapII CCGG 2 cut(s) 1087, 1433
HgaI GACGC 3 cut(s) 312, 409, 589
Hin1II CATG 7 cut(s) 247, 472, 565, 795, 1020, 1084, 1720
HincII GTYRAC 3 cut(s) 360, 1014, 1321
HindII GTYRAC 3 cut(s) 360, 1014, 1321
HindIII AAGCTT 1 cut(s) 1386
HpaI GTTAAC 1 cut(s) 360
HpaII CCGG 2 cut(s) 1087, 1433
HphI GGTGA 9 cut(s) 226, 703, 790, 938, 991, 1051, 1135, 1150, 1522
Hpy166II GTNNAC 3 cut(s) 360, 1014, 1321
Hpy188I TCNGA 9 cut(s) 142, 302, 381, 523, 541, 837, 1236, 1424, 1745
Hpy188III TCNNGA 4 cut(s) 90, 772, 1081, 1679
Hpy8I GTNNAC 3 cut(s) 360, 1014, 1321
Hpy99I CGWCG 2 cut(s) 425, 605
HpyAV CCTTC 9 cut(s) 278, 828, 842, 1066, 1087, 1132, 1241, 1299, 1563
HpyCH4III ACNGT 9 cut(s) 23, 229, 316, 688, 961, 1036, 1267, 1516, 1688
HpyCH4IV ACGT 3 cut(s) 504, 1339, 1494
HpyCH4V TGCA 9 cut(s) 38, 113, 202, 224, 287, 329, 402, 899, 1598
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 338, 1277, 1385
HpyF3I CTNAG 7 cut(s) 124, 132, 141, 508, 538, 1126, 1621
HpySE526I ACGT 3 cut(s) 504, 1339, 1494
Hsp92II CATG 7 cut(s) 247, 472, 565, 795, 1020, 1084, 1720
KflI GGGWCCC 1 cut(s) 1763
KspAI GTTAAC 1 cut(s) 360
Kzo9I GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
LguI GCTCTTC 1 cut(s) 1469
LweI GCATC 2 cut(s) 280, 603
MaeII ACGT 3 cut(s) 504, 1339, 1494
MaeIII GTNAC 6 cut(s) 127, 178, 232, 708, 961, 1510
MalI GATC 7 cut(s) 149, 566, 695, 770, 1277, 1677, 1742
MboI GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 4 cut(s) 138, 251, 1457, 1464
MlyI GAGTC 5 cut(s) 799, 958, 1219, 1245, 1525
MmeI TCCRAC 2 cut(s) 525, 1222
MroXI GAANNNNTTC 1 cut(s) 1178
MseI TTAA 4 cut(s) 206, 359, 761, 1469
MslI CAYNNNNRTG 3 cut(s) 167, 1079, 1481
MspCI CTTAAG 1 cut(s) 1468
MspI CCGG 2 cut(s) 1087, 1433
Mva1269I GAATGC 1 cut(s) 422
MwoI GCNNNNNNNGC 4 cut(s) 221, 338, 1277, 1385
NdeII GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
NlaIII CATG 7 cut(s) 247, 472, 565, 795, 1020, 1084, 1720
NlaIV GGNNCC 5 cut(s) 1099, 1120, 1431, 1764, 1765
NmeAIII GCCGAG 1 cut(s) 680
NmuCI GTSAC 4 cut(s) 178, 232, 961, 1510
NspI RCATGY 1 cut(s) 1020
PaeR7I CTCGAG 1 cut(s) 747
PagI TCATGA 1 cut(s) 1080
PciI ACATGT 1 cut(s) 1016
PciSI GCTCTTC 1 cut(s) 1469
PctI GAATGC 1 cut(s) 422
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 5 cut(s) 256, 533, 838, 859, 876
PleI GAGTC 5 cut(s) 799, 958, 1218, 1245, 1525
PpsI GAGTC 5 cut(s) 799, 958, 1218, 1245, 1525
Ppu21I YACGTR 2 cut(s) 1340, 1495
PpuMI RGGWCCY 1 cut(s) 1763
PscI ACATGT 1 cut(s) 1016
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 1763
PspEI GGTNACC 1 cut(s) 1510
PspFI CCCAGC 1 cut(s) 1379
PspN4I GGNNCC 5 cut(s) 1099, 1120, 1431, 1764, 1765
PspPI GGNCC 1 cut(s) 1763
PspPPI RGGWCCY 1 cut(s) 1763
PspXI VCTCGAGB 1 cut(s) 747
PstI CTGCAG 2 cut(s) 115, 331
PsuI RGATCY 1 cut(s) 147
RsaI GTAC 2 cut(s) 318, 1670
RsaNI GTAC 2 cut(s) 317, 1669
RseI CAYNNNNRTG 3 cut(s) 167, 1079, 1481
SacI GAGCTC 1 cut(s) 138
SapI GCTCTTC 1 cut(s) 1469
SaqAI TTAA 4 cut(s) 206, 359, 761, 1469
Sau3AI GATC 7 cut(s) 147, 564, 693, 768, 1275, 1675, 1740
Sau96I GGNCC 1 cut(s) 1763
SchI GAGTC 5 cut(s) 799, 958, 1219, 1245, 1525
SduI GDGCHC 4 cut(s) 138, 251, 1457, 1464
SfaNI GCATC 2 cut(s) 280, 603
SfcI CTRYAG 2 cut(s) 111, 327
Sfr274I CTCGAG 1 cut(s) 747
SinI GGWCC 1 cut(s) 1763
SlaI CTCGAG 1 cut(s) 747
SmiMI CAYNNNNRTG 3 cut(s) 167, 1079, 1481
SmlI CTYRAG 3 cut(s) 747, 966, 1468
SmoI CTYRAG 3 cut(s) 747, 966, 1468
SnaBI TACGTA 1 cut(s) 1495
SsiI CCGC 2 cut(s) 458, 1284
SspI AATATT 1 cut(s) 649
SstI GAGCTC 1 cut(s) 138
StyI CCWWGG 3 cut(s) 262, 944, 1216
TaaI ACNGT 9 cut(s) 23, 229, 316, 688, 961, 1036, 1267, 1516, 1688
TaiI ACGT 3 cut(s) 507, 1342, 1497
TaqI TCGA 8 cut(s) 14, 67, 81, 423, 733, 748, 888, 1195
TatI WGTACW 2 cut(s) 316, 1668
TauI GCSGC 2 cut(s) 461, 1286
TfiI GAWTC 5 cut(s) 256, 533, 838, 859, 876
Tru1I TTAA 4 cut(s) 206, 359, 761, 1469
Tru9I TTAA 4 cut(s) 206, 359, 761, 1469
TscAI CASTG 4 cut(s) 28, 693, 966, 1693
TseFI GTSAC 4 cut(s) 178, 232, 961, 1510
Tsp45I GTSAC 4 cut(s) 178, 232, 961, 1510
TspDTI ATGAA 9 cut(s) 185, 232, 924, 1069, 1097, 1129, 1408, 1457, 1715
TspRI CASTG 4 cut(s) 28, 693, 966, 1693
Vha464I CTTAAG 1 cut(s) 1468
VpaK11BI GGWCC 1 cut(s) 1763
XapI RAATTY 3 cut(s) 172, 1412, 1615
XceI RCATGY 1 cut(s) 1020
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 747
XmnI GAANNNNTTC 1 cut(s) 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.