FvH4_6g44840

cystathionine gamma-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017349
Physical Location & Seq
Reverse (-)
34500326 .. 34502475
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44840.t1

Sequence Viewer

Length: 1503 bp
ATGGGGGGACCTTACAATCACAACAAGGGTGGGAATAGTAGTAATTCTAGTAGCAACAGAGGGAGGCCTTATGGTTTGATGCTGCTGTTGGCATTTGGGGCTGCATTGGTTGGAGTCATGGTGCTTCATAAGTTCAGAGAAAGGCGCATCTTCAACCTTGTTATCAAAGAGAAAGACACTCAGCTCGTTTCGCTTCACCTCGCCTTACAGAAGGAGAGAGACTACAGCAGGGAAGTAAAAATGAAGCATGAAGAGCTGAAAGCGAAGATGTACTCCATTAGAACACAAAAGATGCAGCTTGAGAGGACGGTGTTCGAGATGAAGTCTACTATTGGTTCGCTTAAAGAGGAGCTGAGAACAATGGAGGCTGCATTTGAGGAGAAGCAAAACGAAACCAAAGTGACGAGATTGAGTGATGATCGGAACGATGATCCTCGGATGATGGAGTTGGTGGAAAGCCTAAAGCAAAAGGAAGCTGAAATTGAGAAGTTGAAACACCACCTCGAGTATAAGGTATGGTCGGTAAGTGCTGACGACACATCAAACCCAACCGGAAATCTGACCATGTCCAGAAGGCGAGGAGTGGCTGATCAAGTGTCAGGTGAAGAAGGAACTGCCAATAAGTCCATGAATCTTAAACAGGGTGAGACTAGGAAAAAAGTAGACGATCAAACTGGCAACGCAGAAGGGGAAACAAAGGTGGATGATTCCCGAGTTGTGAATATGAAAGGTGGCATTACCACTGAGGAGGTCCGAGCAAAGAATCAGGATTTCAAAGAGGGAAATCAGAAAGCAATTGATGGTCAAAAGAGAAGTAGGAATCTCCAAAAAGTTGAAAATTCTGAACAAGGTGATGGACAGAAAATTCGAGTGAAAAAAGAGCGAGGGATGAAGTTGGAAATGCAAGATGTGGGATCTGAAAATAAGGAAAGGTCTAGATTGGGAGGGAAGCAAGACTACTTTAGAGGTATCAAAGGAAAGAAATGGAGATTAGTTCCCAGGAATAGGAGGATGGAGAAGAAGGGGAGTTATCGAAACAATGGGGTGGCAAGCATGAGAGGTAGGAGTTTCTTTGAAGATGATCAGTATAAGGGCAGAGCAGAGGAAGCGGCTTTGAACAGGAGAGTAACAAGCGATGATCGCACAATGCAAGCAAGGGAGTCTGCGGATTCAAGTGATGCAGAAGATCTGGAAAACAAGCTTACACATTTTGATTCAACTCATTCAAGTGATGGTGATGGTAAAGTCAGTGGCACAGCAAGTAATGATACAAAACAGAAACAAGAAGTTGAAAATTCAGAAGACCATGAAGCCGTCAATAATATCGGCAATGAAGAAGATAGAGCTGATGCCGATACACAGGACTTTCCGGGAGATATGGAAATTGCAGAGGCTGAAGAACAGGAGAATGATGTAACTGAGGACGGTTTCCTTGCTGAATCAGGTTTCAATTCGGAAGATAGAGAGGAGTACAAGGAGGAAATAGATGCGTCTGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

501

Amino Acids

56.86

Weight (kDa)

5.56

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017349)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44840
prunus_persica Prupe.3G232500_v2.0.a1
pyrus_communis pycom09g01800
rosa_chinensis RchiOBHm_Chr2g0162021
rosa_laevigata RLG00000021315
rosa_multiflora Rmu_co8492063.1_g000001
rosa_roxburghii Rroxscaffold_2G00088390
rosa_rugosa Rorug02G0494000
rosa_samantha Rh2AG560000 Rh2BG573400 Rh2CG543600 Rh2DG583200
rosa_wichuraiana Rw2G046350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 326, 663
AciI CCGC 2 cut(s) 1109, 1166
AclWI GGATC 2 cut(s) 425, 922
AcsI RAATTY 4 cut(s) 838, 864, 1294, 1496
AcuI CTGAAG 1 cut(s) 1416
AfaI GTAC 2 cut(s) 272, 1472
AfiI CCNNNNNNNGG 1 cut(s) 1005
AjnI CCWGG 1 cut(s) 998
AluBI AGCT 7 cut(s) 184, 256, 298, 352, 476, 1201, 1346
AluI AGCT 7 cut(s) 184, 256, 298, 352, 476, 1201, 1346
Alw26I GTCTC 2 cut(s) 213, 641
AlwI GGATC 2 cut(s) 425, 922
AlwNI CAGNNNCTG 1 cut(s) 1394
Ama87I CYCGRG 2 cut(s) 503, 711
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 4 cut(s) 82, 101, 295, 368
ApoI RAATTY 4 cut(s) 838, 864, 1294, 1496
ArsI GACNNNNNNTTYG 2 cut(s) 849, 881
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 8, 751
AsuC2I CCSGG 1 cut(s) 1371
AsuHPI GGTGA 5 cut(s) 188, 614, 656, 863, 1247
AvaI CYCGRG 2 cut(s) 503, 711
AvaII GGWCC 2 cut(s) 8, 751
BarI GAAGNNNNNNTAC 2 cut(s) 941, 973
BbsI GAAGAC 1 cut(s) 1308
BbvI GCAGC 4 cut(s) 69, 88, 307, 355
BccI CCATC 6 cut(s) 436, 794, 848, 1006, 1226, 1232
BceAI ACGGC 1 cut(s) 1298
BciT130I CCWGG 1 cut(s) 1000
BclI TGATCA 2 cut(s) 589, 1081
BcnI CCSGG 1 cut(s) 1371
BcoDI GTCTC 2 cut(s) 213, 641
BfaI CTAG 4 cut(s) 48, 651, 936, 1501
BfmI CTRYAG 1 cut(s) 223
BglII AGATCT 1 cut(s) 1186
BisI GCNGC 5 cut(s) 83, 102, 296, 369, 1110
BlsI GCNGC 5 cut(s) 84, 103, 297, 370, 1111
Bme1390I CCNGG 2 cut(s) 1000, 1371
Bme18I GGWCC 2 cut(s) 8, 751
BmeT110I CYCGRG 2 cut(s) 503, 711
BmgT120I GGNCC 2 cut(s) 8, 751
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 1000, 1371
BmsI GCATC 6 cut(s) 69, 156, 282, 1168, 1339, 1477
BpiI GAAGAC 1 cut(s) 1308
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 1371
BsaJI CCNNGG 2 cut(s) 434, 998
BsaWI WCCGGW 1 cut(s) 551
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 1005
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 1 cut(s) 1336
BseBI CCWGG 1 cut(s) 1000
BseDI CCNNGG 2 cut(s) 434, 998
BseGI GGATG 4 cut(s) 444, 709, 894, 1017
BseLI CCNNNNNNNGG 1 cut(s) 1005
BseMI GCAATG 1 cut(s) 1336
BseMII CTCAG 4 cut(s) 194, 344, 735, 1410
BseNI ACTGG 1 cut(s) 679
BseRI GAGGAG 5 cut(s) 362, 392, 594, 761, 1481
BseXI GCAGC 4 cut(s) 69, 88, 307, 355
BshFI GGCC 1 cut(s) 67
BsiHKCI CYCGRG 2 cut(s) 503, 711
BsiSI CCGG 2 cut(s) 552, 1370
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 1005
BsmAI GTCTC 2 cut(s) 213, 641
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 1 cut(s) 67
BsoBI CYCGRG 2 cut(s) 503, 711
Bsp143I GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
BspACI CCGC 2 cut(s) 1109, 1166
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 4 cut(s) 193, 345, 736, 1411
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 2 cut(s) 425, 922
BspQI GCTCTTC 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 1336
BsrI ACTGG 1 cut(s) 679
BssECI CCNNGG 2 cut(s) 434, 998
BssMI GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
Bst2UI CCWGG 1 cut(s) 1000
Bst4CI ACNGT 2 cut(s) 310, 1427
Bst6I CTCTTC 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 1051, 1152
BstDEI CTNAG 4 cut(s) 180, 353, 744, 1419
BstF5I GGATG 4 cut(s) 444, 709, 894, 1017
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 8 cut(s) 421, 433, 592, 670, 917, 1084, 1141, 1189
BstMAI GTCTC 2 cut(s) 213, 641
BstMBI GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
BstMWI GCNNNNNNNGC 5 cut(s) 98, 190, 253, 1106, 1140
BstNI CCWGG 1 cut(s) 1000
BstSCI CCNGG 2 cut(s) 998, 1369
BstSFI CTRYAG 1 cut(s) 223
BstV1I GCAGC 4 cut(s) 69, 88, 307, 355
BstV2I GAAGAC 1 cut(s) 1308
BstX2I RGATCY 2 cut(s) 914, 1186
BstYI RGATCY 2 cut(s) 914, 1186
BsuRI GGCC 1 cut(s) 67
BtgZI GCGATG 1 cut(s) 1149
BtsCI GGATG 4 cut(s) 444, 709, 894, 1017
BtsIMutI CAGTG 2 cut(s) 741, 1255
Cac8I GCNNGC 2 cut(s) 1051, 1152
CaiI CAGNNNCTG 1 cut(s) 1394
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 2 cut(s) 8, 751
CseI GACGC 1 cut(s) 1479
Csp6I GTAC 2 cut(s) 271, 1471
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 6 cut(s) 118, 248, 565, 628, 1054, 1307
CviQI GTAC 2 cut(s) 271, 1471
DdeI CTNAG 4 cut(s) 180, 353, 744, 1419
DpnI GATC 8 cut(s) 420, 432, 591, 669, 916, 1083, 1140, 1188
DpnII GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco147I AGGCCT 1 cut(s) 67
Eco47I GGWCC 2 cut(s) 8, 751
Eco57I CTGAAG 1 cut(s) 1416
Eco88I CYCGRG 2 cut(s) 503, 711
EcoO109I RGGNCCY 1 cut(s) 8
EcoRI GAATTC 1 cut(s) 1496
EcoRII CCWGG 1 cut(s) 998
FaeI CATG 6 cut(s) 121, 251, 568, 631, 1057, 1310
FaqI GGGAC 1 cut(s) 21
FatI CATG 6 cut(s) 117, 247, 564, 627, 1053, 1306
FbaI TGATCA 2 cut(s) 589, 1081
FblI GTMKAC 2 cut(s) 326, 663
Fnu4HI GCNGC 5 cut(s) 83, 102, 296, 369, 1110
FokI GGATG 4 cut(s) 451, 716, 901, 1024
Fsp4HI GCNGC 5 cut(s) 83, 102, 296, 369, 1110
FspBI CTAG 4 cut(s) 48, 651, 936, 1501
GlaI GCGC 1 cut(s) 146
GluI GCNGC 5 cut(s) 83, 102, 296, 369, 1110
HaeIII GGCC 1 cut(s) 67
HapII CCGG 2 cut(s) 552, 1370
HgaI GACGC 1 cut(s) 1479
HhaI GCGC 1 cut(s) 147
Hin1II CATG 6 cut(s) 121, 251, 568, 631, 1057, 1310
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 1199
HinfI GANTC 9 cut(s) 114, 631, 707, 763, 820, 1160, 1169, 1214, 1439
HpaII CCGG 2 cut(s) 552, 1370
HphI GGTGA 5 cut(s) 188, 614, 656, 863, 1247
Hpy166II GTNNAC 2 cut(s) 327, 664
Hpy188III TCNNGA 6 cut(s) 316, 570, 711, 767, 936, 1190
Hpy8I GTNNAC 2 cut(s) 327, 664
HpyAV CCTTC 5 cut(s) 205, 567, 602, 680, 1015
HpyCH4III ACNGT 2 cut(s) 310, 1427
HpyCH4V TGCA 7 cut(s) 104, 295, 371, 904, 1150, 1181, 1388
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 190, 253, 1106, 1140
HpyF3I CTNAG 4 cut(s) 180, 353, 744, 1419
Hsp92II CATG 6 cut(s) 121, 251, 568, 631, 1057, 1310
HspAI GCGC 1 cut(s) 145
Ksp22I TGATCA 2 cut(s) 589, 1081
Kzo9I GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 4 cut(s) 69, 88, 307, 355
LweI GCATC 6 cut(s) 69, 156, 282, 1168, 1339, 1477
MaeI CTAG 4 cut(s) 48, 651, 936, 1501
MaeIII GTNAC 3 cut(s) 400, 1126, 1414
MalI GATC 8 cut(s) 420, 432, 591, 669, 916, 1083, 1140, 1188
MboI GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
MfeI CAATTG 1 cut(s) 795
MflI RGATCY 2 cut(s) 914, 1186
MluCI AATT 9 cut(s) 43, 480, 795, 838, 864, 1294, 1383, 1450, 1496
MlyI GAGTC 2 cut(s) 123, 1169
MmeI TCCRAC 2 cut(s) 91, 876
MseI TTAA 2 cut(s) 342, 636
MslI CAYNNNNRTG 1 cut(s) 1227
MspI CCGG 2 cut(s) 552, 1370
MspR9I CCNGG 2 cut(s) 1000, 1371
MunI CAATTG 1 cut(s) 795
MvaI CCWGG 1 cut(s) 1000
MwoI GCNNNNNNNGC 5 cut(s) 98, 190, 253, 1106, 1140
NciI CCSGG 1 cut(s) 1371
NdeII GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
NlaIII CATG 6 cut(s) 121, 251, 568, 631, 1057, 1310
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 503
PceI AGGCCT 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 246
PfeI GAWTC 7 cut(s) 631, 707, 763, 820, 1169, 1214, 1439
PflFI GACNNNGTC 1 cut(s) 565
PfoI TCCNGGA 1 cut(s) 1369
PkrI GCNGC 5 cut(s) 84, 103, 297, 370, 1111
PleI GAGTC 2 cut(s) 122, 1168
PpsI GAGTC 2 cut(s) 122, 1168
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 998
PspGI CCWGG 1 cut(s) 998
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 2 cut(s) 8, 751
PspPPI RGGWCCY 1 cut(s) 8
PspXI VCTCGAGB 1 cut(s) 503
PstNI CAGNNNCTG 1 cut(s) 1394
PsuI RGATCY 2 cut(s) 914, 1186
PsyI GACNNNGTC 1 cut(s) 565
RsaI GTAC 2 cut(s) 272, 1472
RsaNI GTAC 2 cut(s) 271, 1471
RseI CAYNNNNRTG 1 cut(s) 1227
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 2 cut(s) 342, 636
SatI GCNGC 5 cut(s) 83, 102, 296, 369, 1110
Sau3AI GATC 8 cut(s) 418, 430, 589, 667, 914, 1081, 1138, 1186
Sau96I GGNCC 2 cut(s) 8, 751
SchI GAGTC 2 cut(s) 123, 1169
ScrFI CCNGG 2 cut(s) 1000, 1371
SfaNI GCATC 6 cut(s) 69, 156, 282, 1168, 1339, 1477
SfcI CTRYAG 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 503
SinI GGWCC 2 cut(s) 8, 751
SlaI CTCGAG 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 1227
SmlI CTYRAG 2 cut(s) 299, 503
SmoI CTYRAG 2 cut(s) 299, 503
Sse9I AATT 9 cut(s) 43, 480, 795, 838, 864, 1294, 1383, 1450, 1496
SseBI AGGCCT 1 cut(s) 67
SsiI CCGC 2 cut(s) 1109, 1166
SspMI CTAG 4 cut(s) 48, 651, 936, 1501
StuI AGGCCT 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 998, 1369
TaaI ACNGT 2 cut(s) 310, 1427
TaqI TCGA 4 cut(s) 315, 504, 868, 1033
TasI AATT 9 cut(s) 43, 480, 795, 838, 864, 1294, 1383, 1450, 1496
TatI WGTACW 2 cut(s) 270, 1470
TauI GCSGC 1 cut(s) 1112
TfiI GAWTC 7 cut(s) 631, 707, 763, 820, 1169, 1214, 1439
Tru1I TTAA 2 cut(s) 342, 636
Tru9I TTAA 2 cut(s) 342, 636
TscAI CASTG 2 cut(s) 748, 1255
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 4 cut(s) 82, 101, 295, 368
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 9 cut(s) 116, 257, 264, 335, 644, 740, 905, 1323, 1347
TspRI CASTG 2 cut(s) 748, 1255
Tth111I GACNNNGTC 1 cut(s) 565
VpaK11BI GGWCC 2 cut(s) 8, 751
XapI RAATTY 4 cut(s) 838, 864, 1294, 1496
XbaI TCTAGA 1 cut(s) 935
XhoI CTCGAG 1 cut(s) 503
XmiI GTMKAC 2 cut(s) 326, 663
XspI CTAG 4 cut(s) 48, 651, 936, 1501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.