FvH4_6g45320

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017351
Physical Location & Seq
Reverse (-)
34803403 .. 34804511
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45320.t1

Sequence Viewer

Length: 531 bp
ATGACCCCCTTTGGCCCTGCCTTGAAATTTGAACTGTGGCTTTGTGTTCTTAAGCTTTTATCAATGGCGCTTGGAAGAGTTGGCAAAAATTTCATGGCTCCTCTTCTAGCAGTGAACCTGGTGGTTCATGTCATCGTTGTTGGATTGGCTGGTTGGTCACTTGATAAGTACATCGACGGCGAACAAAATCACCCGCATTTGGGAGGGAATACATCGACTAGCTTTATGTTGATCTTTGCTCTTATTGCTGGAACGTTTGGTGCTTGCTCTGTGATCATAGGCATGATGCATCTACGGGCATGGCAGCACGAAAGCCATGCTGCTGCAGCTTCTGTAGCCATCATTTCCTGGGCTCTGATAGCACTTGCTTTCGGTGTTGTATGCAAGGAGGTCATATTAGGGGGACACCGAGGAAAACGATTGCAAACGTTGGAACTTTTTATTGCAATCTCACTGCTAAGTCAATTGCTGTACTTGGTGCTACTGAATACTGGGATGTTCAAGAGGAGATATGGACCAACTGTTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

177

Amino Acids

19.11

Weight (kDa)

9.55

Isoelectric Point (pI)

26.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 38 - 165 6.6e-38 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45320
malus_domestica MD09G1085800.v1.1 MD17G1074700.v1.1
rosa_chinensis RchiOBHm_Chr2g0163081
rosa_laevigata RLG00000021372
rosa_multiflora Rmu_sc0003984.1_g000006
rosa_roxburghii Rroxscaffold_2G00087560
rosa_rugosa Rorug02G0500200 Rorug02G0500200
rosa_samantha Rh2AG566600 Rh2BG579000 Rh2CG548900
rosa_wichuraiana Rw2G046910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 194
AclI AACGTT 2 cut(s) 254, 428
AcsI RAATTY 2 cut(s) 26, 88
AfaI GTAC 2 cut(s) 170, 473
AfiI CCNNNNNNNGG 2 cut(s) 199, 200
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 3 cut(s) 25, 32, 502
AjnI CCWGG 2 cut(s) 117, 347
AluBI AGCT 3 cut(s) 55, 222, 329
AluI AGCT 3 cut(s) 55, 222, 329
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 4 cut(s) 304, 320, 323, 326
ApoI RAATTY 2 cut(s) 26, 88
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 2 cut(s) 14, 515
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 355
BbvI GCAGC 4 cut(s) 307, 310, 316, 338
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 193
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 2 cut(s) 119, 349
BclI TGATCA 1 cut(s) 273
BfaI CTAG 2 cut(s) 107, 219
BfmI CTRYAG 2 cut(s) 324, 333
BfoI RGCGCY 1 cut(s) 71
BfrI CTTAAG 1 cut(s) 50
BisI GCNGC 4 cut(s) 305, 321, 324, 327
BlsI GCNGC 4 cut(s) 306, 322, 325, 328
Bme1390I CCNGG 2 cut(s) 119, 349
Bme18I GGWCC 1 cut(s) 515
BmgT120I GGNCC 2 cut(s) 14, 515
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 2 cut(s) 119, 349
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 2 cut(s) 276, 298
BmuI ACTGGG 1 cut(s) 501
BsaJI CCNNGG 2 cut(s) 348, 409
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 200
Bse1I ACTGG 1 cut(s) 496
BseBI CCWGG 2 cut(s) 119, 349
BseDI CCNNGG 2 cut(s) 348, 409
BseGI GGATG 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 199, 200
BseNI ACTGG 1 cut(s) 496
BseRI GAGGAG 2 cut(s) 90, 520
BseXI GCAGC 4 cut(s) 307, 310, 316, 338
BshFI GGCC 1 cut(s) 15
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 2 cut(s) 199, 200
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 2 cut(s) 231, 273
BspACI CCGC 1 cut(s) 194
BspANI GGCC 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 99
BspMAI CTGCAG 1 cut(s) 328
BspTI CTTAAG 1 cut(s) 50
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 348, 409
BssMI GATC 2 cut(s) 231, 273
Bst2UI CCWGG 2 cut(s) 119, 349
Bst4CI ACNGT 2 cut(s) 36, 523
Bst6I CTCTTC 2 cut(s) 70, 108
BstAFI CTTAAG 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 458
BstF5I GGATG 1 cut(s) 501
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 2 cut(s) 234, 276
BstMBI GATC 2 cut(s) 231, 273
BstMWI GCNNNNNNNGC 4 cut(s) 245, 326, 335, 359
BstNI CCWGG 2 cut(s) 119, 349
BstSCI CCNGG 2 cut(s) 117, 347
BstSFI CTRYAG 2 cut(s) 324, 333
BstV1I GCAGC 4 cut(s) 307, 310, 316, 338
BsuRI GGCC 1 cut(s) 15
BtsCI GGATG 1 cut(s) 501
BtsI GCAGTG 2 cut(s) 117, 452
BtsIMutI CAGTG 2 cut(s) 117, 452
Cac8I GCNNGC 1 cut(s) 265
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 1 cut(s) 70
Cfr13I GGNCC 2 cut(s) 14, 515
CsiI ACCWGGT 1 cut(s) 117
Csp6I GTAC 2 cut(s) 169, 472
CviAII CATG 5 cut(s) 94, 128, 283, 300, 317
CviQI GTAC 2 cut(s) 169, 472
DdeI CTNAG 1 cut(s) 458
DpnI GATC 2 cut(s) 233, 275
DpnII GATC 2 cut(s) 231, 273
Eam1104I CTCTTC 2 cut(s) 70, 108
EarI CTCTTC 2 cut(s) 70, 108
Eco24I GRGCYC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 117, 347
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 355
FaeI CATG 5 cut(s) 97, 131, 286, 303, 320
FaqI GGGAC 1 cut(s) 417
FatI CATG 5 cut(s) 93, 127, 282, 299, 316
FauI CCCGC 1 cut(s) 201
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 4 cut(s) 305, 321, 324, 327
FokI GGATG 1 cut(s) 508
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 4 cut(s) 305, 321, 324, 327
FspBI CTAG 2 cut(s) 107, 219
GlaI GCGC 1 cut(s) 69
GluI GCNGC 4 cut(s) 305, 321, 324, 327
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 1 cut(s) 15
HhaI GCGC 1 cut(s) 70
Hin1II CATG 5 cut(s) 97, 131, 286, 303, 320
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HindIII AAGCTT 1 cut(s) 53
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 357
Hpy188III TCNNGA 1 cut(s) 502
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 2 cut(s) 36, 523
HpyCH4IV ACGT 2 cut(s) 254, 428
HpyCH4V TGCA 5 cut(s) 289, 326, 384, 424, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 326, 335, 359
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 2 cut(s) 254, 428
Hsp92II CATG 5 cut(s) 97, 131, 286, 303, 320
HspAI GCGC 1 cut(s) 68
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 2 cut(s) 231, 273
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 8 cut(s) 30, 104, 131, 135, 234, 334, 361, 477
Lsp1109I GCAGC 4 cut(s) 307, 310, 316, 338
LweI GCATC 2 cut(s) 276, 298
MabI ACCWGGT 1 cut(s) 117
MaeI CTAG 2 cut(s) 107, 219
MaeII ACGT 2 cut(s) 254, 428
MaeIII GTNAC 1 cut(s) 156
MalI GATC 2 cut(s) 233, 275
MboI GATC 2 cut(s) 231, 273
MboII GAAGA 2 cut(s) 87, 95
MfeI CAATTG 1 cut(s) 464
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 3 cut(s) 26, 88, 464
MmeI TCCRAC 2 cut(s) 121, 411
MnlI CCTC 5 cut(s) 111, 197, 382, 404, 498
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 281
MspCI CTTAAG 1 cut(s) 50
MspR9I CCNGG 2 cut(s) 119, 349
MunI CAATTG 1 cut(s) 464
MvaI CCWGG 2 cut(s) 119, 349
MwoI GCNNNNNNNGC 4 cut(s) 245, 326, 335, 359
NdeII GATC 2 cut(s) 231, 273
NlaIII CATG 5 cut(s) 97, 131, 286, 303, 320
NlaIV GGNNCC 1 cut(s) 99
NmuCI GTSAC 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 291
PkrI GCNGC 4 cut(s) 306, 322, 325, 328
Psp1406I AACGTT 2 cut(s) 254, 428
Psp6I CCWGG 2 cut(s) 117, 347
PspGI CCWGG 2 cut(s) 117, 347
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 2 cut(s) 14, 515
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 170, 473
RsaNI GTAC 2 cut(s) 169, 472
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 4 cut(s) 305, 321, 324, 327
Sau3AI GATC 2 cut(s) 231, 273
Sau96I GGNCC 2 cut(s) 14, 515
ScrFI CCNGG 2 cut(s) 119, 349
SduI GDGCHC 1 cut(s) 355
SetI ASST 7 cut(s) 57, 120, 224, 257, 331, 393, 431
SexAI ACCWGGT 1 cut(s) 117
SfaNI GCATC 2 cut(s) 276, 298
SfcI CTRYAG 2 cut(s) 324, 333
SinI GGWCC 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 3 cut(s) 26, 88, 464
SsiI CCGC 1 cut(s) 194
SspMI CTAG 2 cut(s) 107, 219
StyD4I CCNGG 2 cut(s) 117, 347
TaaI ACNGT 2 cut(s) 36, 523
TaiI ACGT 2 cut(s) 257, 431
TaqI TCGA 2 cut(s) 174, 215
TasI AATT 3 cut(s) 26, 88, 464
TatI WGTACW 2 cut(s) 168, 471
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 2 cut(s) 117, 459
TseFI GTSAC 1 cut(s) 156
TseI GCWGC 4 cut(s) 304, 320, 323, 326
Tsp45I GTSAC 1 cut(s) 156
TspDTI ATGAA 2 cut(s) 82, 116
TspRI CASTG 2 cut(s) 117, 459
Vha464I CTTAAG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 515
XapI RAATTY 2 cut(s) 26, 88
XspI CTAG 2 cut(s) 107, 219
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.