FvH4_6g45660

twin BRCT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004814
Physical Location & Seq
Reverse (-)
34999115 .. 35003881
4767 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45660.t2

Sequence Viewer

Length: 1470 bp
ATGGCATCGGATCCAATTCCAGGCATGGAGTCGGTGGTGGCCTCCGTCACCGGGTACCGCGGATCGGAGCTGGAGAATCTGATCGAGCTCATGACTTTTGCAGGTGCCAGCTATATCGGTCGCTTGTCGCCTTCTAATGCCTTCACTCATTTGGTGTGCTGGAAATTTGAGGGGAAGAAATATGAAATGGCTATAAAGTTGAATCTCATAATAGTTAACCACCGCTGGATTGAGGACTGCGTAAAGCATCGAAAGCGGCTTCCTGAGCATCCCTATACCTTGCAAAGTGGACAGGAAGTGGGACCCTTACAGTTGAAAGTTCCGGTTGTTGCTAAACCAGGCTCCTCGGTTAAGAAGGGGAAAGCAGTTCTTGATATTTCTCATGTTTGTCGCAACTCTGAAGAAATTATCGATCTGGGGTGTGGATCTTCTCGACGTGGTGTGTTTGCTGATTTACGTCTATTGAATGAGAATTCGTTTCCTGAATTTAAAGAAAAAAATAGTTCCCAGAAGCGACAAAATAAATCTGTGAAAACAAATTCAAAGAAAGAGCTACAATCTAGTAGCAGCGACTGTTTTGAGGATCCTCCTTTATCCAGATCACTCAGAATTGAGGAATCTTGCAGGCATTCAGTTACAGCTGAAAGAAATATTCCCAGTGACAATGGAATCAGCATGGCTGAAATTCCACGTAAGAAAAGGAGGCTTGTGAAAAGAGTATTTGGCTCTACAAGATCGGTACTCTCAGACTCTGATCAGGAATGCCACCCAACCAGATTTCACAGAATCCATAATGACAGGGTCACAATCCTTTCTGATGATTCTAATGACGAGTTAGACACTGGAAGAGGAATCAGAGATGAGGAACGTGAAGGTGTTGAGGTTGGTGGTAATCACTTATCTACTTCCAAGGATCCCAATTTATCTGTTGTGGATGGACCTACAGCTACAGAGGTAACTCCACACAAAGGGTGCCTTGAAGTAGATAAATCAAAAGAAGAATTGGAAATCGGGTTGGACAGTGAACATGCCACCAAAAGTCTTCCACAGGAACTTTCGTGTGTTATATGCTGGACAGAGTTTAGTACAACCCGAGGAATTTTGCCATGTGGTCATCGATACTGTTATCCTTGTATTGAAAATTGGGCTGATCAACTGAGTTCAAGGAGAAAGATTTCAACATGCCCTTTATGCAAAGCTTCATTTACTGTCATTACCAAGGTTGATGATGCAGTTACTAGTGATCAAAATATATTTTCTCAAACAATTCCATCTGCCCCAAAAGTAGACATTTCATTTCTTAATGACCAGGGAACATCGAATTTTGCTGCTCAGATCTTAACTGTTCCCCCTCCTTTTATGGAGTGTACTAAACCATTCATGGACTGTCAAAGCAATGGAATCCGTATCAGCAAATGGTTGTTCCGAGTGTCATTCTTGGGAACCGGAAGAGCTGCTTATCAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00590 ko04918 map00480 map00590 map04918
Pfam Domains
Protein Families
PTCB-BRCT zf-C3HC4 zf-RING_2

Protein Analysis

490

Amino Acids

54.51

Weight (kDa)

7.51

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTCB-BRCT PF12738 12 - 76 1.8e-16 twin BRCT domain
BRCT PF00533 13 - 81 1.7e-08 BRCA1 C Terminus (BRCT) domain
zf-C3HC4_3 PF13920 351 - 402 3.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 353 - 399 1.5e-08 Ring finger domain
zf-C3HC4 PF00097 354 - 398 1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 354 - 396 3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 92
Acc65I GGTACC 1 cut(s) 54
AccB1I GGYRCC 3 cut(s) 54, 104, 972
AccI GTMKAC 1 cut(s) 1287
AccII CGCG 1 cut(s) 60
AciI CCGC 4 cut(s) 58, 60, 223, 256
AclWI GGATC 8 cut(s) 5, 18, 70, 433, 578, 591, 908, 921
AcsI RAATTY 7 cut(s) 164, 472, 485, 538, 684, 1098, 1321
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 4 cut(s) 56, 741, 1087, 1369
AfiI CCNNNNNNNGG 4 cut(s) 20, 51, 64, 968
AgsI TTSAA 8 cut(s) 202, 316, 466, 543, 980, 1139, 1164, 1179
AhlI ACTAGT 1 cut(s) 1238
AjiI CACGTC 1 cut(s) 437
AjnI CCWGG 3 cut(s) 19, 337, 1308
AluBI AGCT 8 cut(s) 70, 88, 111, 553, 641, 947, 1199, 1454
AluI AGCT 8 cut(s) 70, 88, 111, 553, 641, 947, 1199, 1454
Alw21I GWGCWC 1 cut(s) 90
AlwI GGATC 8 cut(s) 5, 18, 70, 433, 578, 591, 908, 921
AlwNI CAGNNNCTG 2 cut(s) 573, 752
Ama87I CYCGRG 1 cut(s) 1092
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 3 cut(s) 567, 1328, 1454
ApoI RAATTY 7 cut(s) 164, 472, 485, 538, 684, 1098, 1321
Asp700I GAANNNNTTC 1 cut(s) 1174
Asp718I GGTACC 1 cut(s) 54
AspS9I GGNCC 2 cut(s) 302, 938
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 1092
AvaII GGWCC 2 cut(s) 302, 938
BamHI GGATCC 3 cut(s) 10, 583, 913
BanI GGYRCC 3 cut(s) 54, 104, 972
BanII GRGCYC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 1034
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 579, 1315, 1441
BccI CCATC 2 cut(s) 929, 1279
BciT130I CCWGG 3 cut(s) 21, 339, 1310
BclI TGATCA 3 cut(s) 754, 1150, 1243
BcnI CCSGG 1 cut(s) 52
BcuI ACTAGT 1 cut(s) 1238
BfaI CTAG 2 cut(s) 561, 1239
BfmI CTRYAG 2 cut(s) 942, 948
BfuAI ACCTGC 1 cut(s) 92
BglII AGATCT 1 cut(s) 1335
BisI GCNGC 4 cut(s) 257, 568, 1329, 1455
BlsI GCNGC 4 cut(s) 258, 569, 1330, 1456
Bme1390I CCNGG 4 cut(s) 21, 52, 339, 1310
Bme18I GGWCC 2 cut(s) 302, 938
BmeT110I CYCGRG 1 cut(s) 1092
BmgBI CACGTC 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 302, 938
BmrFI CCNGG 4 cut(s) 21, 52, 339, 1310
BmrI ACTGGG 1 cut(s) 651
BmsI GCATC 4 cut(s) 14, 256, 277, 1219
BmuI ACTGGG 1 cut(s) 651
BpiI GAAGAC 1 cut(s) 1034
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 52
Bsa29I ATCGAT 2 cut(s) 411, 1117
BsaAI YACGTR 1 cut(s) 692
BsaJI CCNNGG 6 cut(s) 58, 345, 909, 1093, 1218, 1309
BsaWI WCCGGW 2 cut(s) 322, 1445
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 51, 64, 968
Bse1I ACTGG 2 cut(s) 657, 847
Bse3DI GCAATG 1 cut(s) 1402
BseBI CCWGG 3 cut(s) 21, 339, 1310
BseCI ATCGAT 2 cut(s) 411, 1117
BseDI CCNNGG 6 cut(s) 58, 345, 909, 1093, 1218, 1309
BseGI GGATG 2 cut(s) 268, 940
BseLI CCNNNNNNNGG 4 cut(s) 20, 51, 64, 968
BseMI GCAATG 1 cut(s) 1402
BseMII CTCAG 5 cut(s) 255, 619, 759, 1148, 1346
BseNI ACTGG 2 cut(s) 657, 847
BseRI GAGGAG 1 cut(s) 334
BseXI GCAGC 3 cut(s) 579, 1315, 1441
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 3 cut(s) 54, 104, 972
BshVI ATCGAT 2 cut(s) 411, 1117
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 1092
BsiSI CCGG 3 cut(s) 51, 323, 1446
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 4 cut(s) 20, 51, 64, 968
BsmFI GGGAC 1 cut(s) 315
BsmI GAATGC 2 cut(s) 628, 767
BsnI GGCC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 1092
Bsp1286I GDGCHC 1 cut(s) 90
BspACI CCGC 4 cut(s) 58, 60, 223, 256
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 5 cut(s) 256, 618, 758, 1149, 1345
BspDI ATCGAT 2 cut(s) 411, 1117
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 90
BspMI ACCTGC 1 cut(s) 92
BspPI GGATC 8 cut(s) 5, 18, 70, 433, 578, 591, 908, 921
BspQI GCTCTTC 1 cut(s) 1444
BspT107I GGYRCC 3 cut(s) 54, 104, 972
BsrDI GCAATG 1 cut(s) 1402
BsrI ACTGG 2 cut(s) 657, 847
BssECI CCNNGG 6 cut(s) 58, 345, 909, 1093, 1218, 1309
BssT1I CCWWGG 2 cut(s) 909, 1218
Bst2UI CCWGG 3 cut(s) 21, 339, 1310
Bst4CI ACNGT 7 cut(s) 312, 575, 1022, 1124, 1210, 1345, 1388
Bst6I CTCTTC 2 cut(s) 841, 1444
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 2 cut(s) 109, 626
BstDEI CTNAG 5 cut(s) 264, 605, 745, 1157, 1332
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 2 cut(s) 268, 940
BstFNI CGCG 1 cut(s) 60
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 3 cut(s) 253, 265, 1191
BstNI CCWGG 3 cut(s) 21, 339, 1310
BstNSI RCATGY 2 cut(s) 1031, 1185
BstSCI CCNGG 4 cut(s) 19, 50, 337, 1308
BstSFI CTRYAG 2 cut(s) 942, 948
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 579, 1315, 1441
BstV2I GAAGAC 1 cut(s) 1034
BstX2I RGATCY 5 cut(s) 10, 425, 583, 913, 1335
BstYI RGATCY 5 cut(s) 10, 425, 583, 913, 1335
Bsu15I ATCGAT 2 cut(s) 411, 1117
BsuRI GGCC 1 cut(s) 41
BsuTUI ATCGAT 2 cut(s) 411, 1117
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 1 cut(s) 437
BtsCI GGATG 2 cut(s) 268, 940
BtsIMutI CAGTG 3 cut(s) 664, 840, 1027
BveI ACCTGC 1 cut(s) 92
Cac8I GCNNGC 2 cut(s) 109, 626
CaiI CAGNNNCTG 2 cut(s) 573, 752
CciI TCATGA 1 cut(s) 90
Cfr13I GGNCC 2 cut(s) 302, 938
Cfr42I CCGCGG 1 cut(s) 61
ClaI ATCGAT 2 cut(s) 411, 1117
Csp6I GTAC 4 cut(s) 55, 740, 1086, 1368
CviAII CATG 8 cut(s) 25, 91, 383, 676, 1028, 1107, 1182, 1381
CviQI GTAC 4 cut(s) 55, 740, 1086, 1368
DdeI CTNAG 5 cut(s) 264, 605, 745, 1157, 1332
DraI TTTAAA 1 cut(s) 490
Eam1104I CTCTTC 2 cut(s) 841, 1444
EarI CTCTTC 2 cut(s) 841, 1444
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 2 cut(s) 909, 1218
Eco24I GRGCYC 1 cut(s) 90
Eco47I GGWCC 2 cut(s) 302, 938
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 1092
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 472
EcoRII CCWGG 3 cut(s) 19, 337, 1308
EcoT14I CCWWGG 2 cut(s) 909, 1218
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 2 cut(s) 909, 1218
FaeI CATG 8 cut(s) 28, 94, 386, 679, 1031, 1110, 1185, 1384
FalI AAGNNNNNCTT 5 cut(s) 354, 386, 960, 992, 1441
FaqI GGGAC 1 cut(s) 315
FatI CATG 8 cut(s) 24, 90, 382, 675, 1027, 1106, 1181, 1380
FbaI TGATCA 3 cut(s) 754, 1150, 1243
FblI GTMKAC 1 cut(s) 1287
Fnu4HI GCNGC 4 cut(s) 257, 568, 1329, 1455
FokI GGATG 2 cut(s) 255, 947
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 4 cut(s) 257, 568, 1329, 1455
FspBI CTAG 2 cut(s) 561, 1239
GluI GCNGC 4 cut(s) 257, 568, 1329, 1455
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 1 cut(s) 41
HapII CCGG 3 cut(s) 51, 323, 1446
Hin1II CATG 8 cut(s) 28, 94, 386, 679, 1031, 1110, 1185, 1384
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 1197
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 3 cut(s) 51, 323, 1446
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 5 cut(s) 217, 290, 1025, 1288, 1368
Hpy188III TCNNGA 7 cut(s) 91, 263, 371, 432, 482, 597, 758
Hpy8I GTNNAC 5 cut(s) 217, 290, 1025, 1288, 1368
Hpy99I CGWCG 1 cut(s) 438
HpyAV CCTTC 4 cut(s) 141, 151, 349, 866
HpyCH4III ACNGT 7 cut(s) 312, 575, 1022, 1124, 1210, 1345, 1388
HpyCH4IV ACGT 4 cut(s) 436, 457, 691, 868
HpyCH4V TGCA 5 cut(s) 101, 283, 624, 1194, 1232
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 265, 1191
HpyF3I CTNAG 5 cut(s) 264, 605, 745, 1157, 1332
HpySE526I ACGT 4 cut(s) 436, 457, 691, 868
Hsp92II CATG 8 cut(s) 28, 94, 386, 679, 1031, 1110, 1185, 1384
KflI GGGWCCC 1 cut(s) 302
KpnI GGTACC 1 cut(s) 58
Ksp22I TGATCA 3 cut(s) 754, 1150, 1243
KspAI GTTAAC 1 cut(s) 217
KspI CCGCGG 1 cut(s) 61
LguI GCTCTTC 1 cut(s) 1444
LmnI GCTCC 2 cut(s) 67, 347
Lsp1109I GCAGC 3 cut(s) 579, 1315, 1441
LweI GCATC 4 cut(s) 14, 256, 277, 1219
MaeI CTAG 2 cut(s) 561, 1239
MaeII ACGT 4 cut(s) 436, 457, 691, 868
MaeIII GTNAC 6 cut(s) 46, 634, 659, 802, 955, 1234
MboII GAAGA 7 cut(s) 187, 413, 420, 858, 1010, 1034, 1461
MflI RGATCY 5 cut(s) 10, 425, 583, 913, 1335
MhlI GDGCHC 1 cut(s) 90
MlyI GAGTC 2 cut(s) 38, 743
MmeI TCCRAC 1 cut(s) 996
MroXI GAANNNNTTC 1 cut(s) 1174
MseI TTAA 5 cut(s) 216, 351, 489, 1302, 1340
MspA1I CMGCKG 3 cut(s) 60, 225, 641
MspI CCGG 3 cut(s) 51, 323, 1446
MspR9I CCNGG 4 cut(s) 21, 52, 339, 1310
Mva1269I GAATGC 2 cut(s) 628, 767
MvaI CCWGG 3 cut(s) 21, 339, 1310
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 253, 265, 1191
NciI CCSGG 1 cut(s) 52
NlaIII CATG 8 cut(s) 28, 94, 386, 679, 1031, 1110, 1185, 1384
NmuCI GTSAC 3 cut(s) 46, 659, 802
NspI RCATGY 2 cut(s) 1031, 1185
PagI TCATGA 1 cut(s) 90
PaqCI CACCTGC 1 cut(s) 92
PciSI GCTCTTC 1 cut(s) 1444
PctI GAATGC 2 cut(s) 628, 767
PdmI GAANNNNTTC 1 cut(s) 1174
PfeI GAWTC 8 cut(s) 76, 202, 617, 669, 786, 821, 852, 1401
PflFI GACNNNGTC 1 cut(s) 800
PkrI GCNGC 4 cut(s) 258, 569, 1330, 1456
PleI GAGTC 2 cut(s) 37, 743
PpsI GAGTC 2 cut(s) 37, 743
Ppu21I YACGTR 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 302
Psp124BI GAGCTC 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 3 cut(s) 19, 337, 1308
PspGI CCWGG 3 cut(s) 19, 337, 1308
PspPI GGNCC 2 cut(s) 302, 938
PspPPI RGGWCCY 1 cut(s) 302
PstNI CAGNNNCTG 2 cut(s) 573, 752
PsuI RGATCY 5 cut(s) 10, 425, 583, 913, 1335
PsyI GACNNNGTC 1 cut(s) 800
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 4 cut(s) 56, 741, 1087, 1369
RsaNI GTAC 4 cut(s) 55, 740, 1086, 1368
SacI GAGCTC 1 cut(s) 90
SacII CCGCGG 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 1444
SaqAI TTAA 5 cut(s) 216, 351, 489, 1302, 1340
SatI GCNGC 4 cut(s) 257, 568, 1329, 1455
Sau96I GGNCC 2 cut(s) 302, 938
SchI GAGTC 2 cut(s) 38, 743
ScrFI CCNGG 4 cut(s) 21, 52, 339, 1310
SduI GDGCHC 1 cut(s) 90
SfaNI GCATC 4 cut(s) 14, 256, 277, 1219
SfcI CTRYAG 2 cut(s) 942, 948
Sfr303I CCGCGG 1 cut(s) 61
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 2 cut(s) 302, 938
SpeI ACTAGT 1 cut(s) 1238
SsiI CCGC 4 cut(s) 58, 60, 223, 256
SspI AATATT 1 cut(s) 652
SspMI CTAG 2 cut(s) 561, 1239
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 4 cut(s) 19, 50, 337, 1308
StyI CCWWGG 2 cut(s) 909, 1218
TaaI ACNGT 7 cut(s) 312, 575, 1022, 1124, 1210, 1345, 1388
TaiI ACGT 4 cut(s) 439, 460, 694, 871
TaqI TCGA 6 cut(s) 84, 250, 411, 433, 1117, 1319
TaqII GACCGA 1 cut(s) 107
TatI WGTACW 2 cut(s) 1085, 1367
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 8 cut(s) 76, 202, 617, 669, 786, 821, 852, 1401
Tru1I TTAA 5 cut(s) 216, 351, 489, 1302, 1340
Tru9I TTAA 5 cut(s) 216, 351, 489, 1302, 1340
TscAI CASTG 3 cut(s) 664, 847, 1027
TseFI GTSAC 3 cut(s) 46, 659, 802
TseI GCWGC 3 cut(s) 567, 1328, 1454
Tsp45I GTSAC 3 cut(s) 46, 659, 802
TspDTI ATGAA 4 cut(s) 198, 1191, 1284, 1369
TspGWI ACGGA 2 cut(s) 34, 1394
TspRI CASTG 3 cut(s) 664, 847, 1027
Tth111I GACNNNGTC 1 cut(s) 800
VpaK11BI GGWCC 2 cut(s) 302, 938
XapI RAATTY 7 cut(s) 164, 472, 485, 538, 684, 1098, 1321
XceI RCATGY 2 cut(s) 1031, 1185
XmiI GTMKAC 1 cut(s) 1287
XmnI GAANNNNTTC 1 cut(s) 1174
XspI CTAG 2 cut(s) 561, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.