MD17G1072100.v1.1

twin BRCT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004814
Physical Location & Seq
Forward (+)
5787078 .. 5791172
4095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1072100.v1.1.491

Sequence Viewer

Length: 1614 bp
ATGGCAGAGCAACGCCACCCGCTTCGCCTCCCCCAATCTCCCGAGGCCATGGGGTCACCGATTCAGGGCATGGAATCCGTGATCGCCACCGTAAGCGGGTACCACGGCTCGGAGCGGTTCAACCTCATCAAGCTCATTTCTCGCGCCGGTGCGAGCTATGTCGGCGCGATGTCTCGATCGACCACTCACTTGGTGTGTTGGAAATTCGAAGGAAGGAAATACGAACTAGCTAACAAGTTCGACGCGAAAGTTGTCAACCACCGGTGGATTGAGGAGTGCATAAAGCAAGGAAAACGAGTACCTGAGCGTCCTTACACTTTGAGATGTGGACAGGAAGTCGGACCCTTACTCTTACAACCTCCACCTGTTGTTACAGTGGGTGAATCATCGAAAAAATGGAATGCGCTTTCGGACAAATCAAATGGTTATGATGACTCGGTAAAAATTATTGATTCAGAGTCTGGAGCTTCTCGCCGTCATCTTTTGGCTGAATCACGTCTTTTGGATGAGAATTTGTTTCCTGCGTTTAAAGAAAACAGCAGTTCTCATAAGGCAAAGCAGAAACATGTTGGAAGGAATTCGAAGCAAGAACTGCGATCAAGTAGCAGGCACTGTCTTGAAGATCCTCCTCTAACTAGATCACTTGGAAGGGAGGAGAAGGTTCAAGAGTCTTCCACCCATTTGGTGAGAGCTAAAAGAAACATTTTCAGTGGTAAGGAAAGCAGCATGGCTGAAACTTCACGTAAAGGGAGGAAGCTTATAAAAAGGAATATTGGTAGACGTATGGTAGAATCAGAACTCTCAGAGTCTGATCAAGAGTGCCACCCAATCAGATTTCAGAGTACACGTAATGATGTAATCACAGTCCTTTCTGACGATTCAGATGAAGAAAGAAATGATAATATACTGGAAACTGAAAGAGATACTGATAACGGGTTGTATATTGGAAGAAGAATCAGAAATGGTCTGAAAGGTGTTGAGGAGGCTGATCGGAATCACCCATCTTCTTCCAAGCATCCAAATATTTCAGTTGAGGATGAACCCACTGCTCTGGAGGAAACTCCCCGAGATGAATGGTTTGAAGTTGAGAACTCAAAAGAGGAATGTGAAGGCAGATTTGAGACTGAAGCTGCCACCAAAATTCCTTCGATGGAGTTATCATGTGTAATATGCTGGACAGAATATAGTTCAATTCGAGGAATTTTGCCATGTGGTCATCGATTCTGTTATTCATGCATTGAAAACTGGGCTGGTCATATGAGTTCAAGGAGAAAGAATTCAACTTGCCCTTTATGCAAGGCTAGTTTTACCTGCATTACCAAGGTTGATGATGCAGACATGATTGATCAGAAAATATACTCCCAAACCATTCCATCTGCCCCAAAAATGGACATACCAGTGCCTACTTACCGAGGACCACCCAATTTTTCTGCTCCGTCCATATCAGCAACTGCTTGTTCTGTATGTCGTCAGCGGGAACCTGTAGATCTTCTGTTCAGTTGTGACGTCTGCTGCATTCGGTGCATCCATAACTTCTGCCTGGACCCTCCTTTGTTTCCCTGGACATGCATTCACTGCAAGGACCTCCGACGGCTCTACCTTCACAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00590 ko04918 map00480 map00590 map04918
Pfam Domains
Protein Families
PTCB-BRCT zf-C3HC4 zf-RING_2

Protein Analysis

538

Amino Acids

60.87

Weight (kDa)

7.75

Isoelectric Point (pI)

68.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTCB-BRCT PF12738 28 - 89 1.1e-19 twin BRCT domain
BRCT PF00533 28 - 94 1.1e-10 BRCA1 C Terminus (BRCT) domain
zf-C3HC4_3 PF13920 386 - 436 3.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 387 - 433 4.2e-09 Ring finger domain
zf-C3HC4 PF00097 388 - 432 1.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 388 - 430 4.8e-07 RING-type zinc-finger
zf-RING_5 PF14634 388 - 433 8.1e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
AatII GACGTC 1 cut(s) 1509
Acc36I ACCTGC 1 cut(s) 1319
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccBSI CCGCTC 1 cut(s) 115
AccI GTMKAC 1 cut(s) 778
AccII CGCG 3 cut(s) 144, 167, 245
AciI CCGC 4 cut(s) 20, 96, 115, 1474
AclWI GGATC 1 cut(s) 617
AcsI RAATTY 6 cut(s) 203, 511, 577, 1140, 1200, 1276
AcuI CTGAAG 1 cut(s) 1146
AcyI GRCGYC 1 cut(s) 1506
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 3 cut(s) 101, 300, 844
AfiI CCNNNNNNNGG 4 cut(s) 65, 96, 109, 1387
AflIII ACRYGT 2 cut(s) 565, 845
AgeI ACCGGT 1 cut(s) 261
AgsI TTSAA 8 cut(s) 121, 620, 665, 1082, 1191, 1241, 1266, 1281
AhdI GACNNNNNGTC 1 cut(s) 335
AjiI CACGTC 1 cut(s) 497
AjnI CCWGG 2 cut(s) 1539, 1559
AluBI AGCT 8 cut(s) 133, 156, 230, 467, 692, 757, 1130, 1608
AluI AGCT 8 cut(s) 133, 156, 230, 467, 692, 757, 1130, 1608
Alw26I GTCTC 2 cut(s) 177, 1115
AlwI GGATC 1 cut(s) 617
AlwNI CAGNNNCTG 4 cut(s) 461, 612, 809, 1451
Ama87I CYCGRG 2 cut(s) 41, 1065
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 723, 1130, 1512
ApoI RAATTY 6 cut(s) 203, 511, 577, 1140, 1200, 1276
AsiGI ACCGGT 1 cut(s) 261
Asp700I GAANNNNTTC 2 cut(s) 577, 1276
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 3 cut(s) 146, 167, 406
AspS9I GGNCC 4 cut(s) 341, 1415, 1543, 1582
AsuHPI GGTGA 4 cut(s) 48, 392, 697, 989
AsuII TTCGAA 2 cut(s) 207, 581
AvaI CYCGRG 2 cut(s) 41, 1065
AvaII GGWCC 4 cut(s) 341, 1415, 1543, 1582
BanI GGYRCC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 663
BbvI GCAGC 3 cut(s) 735, 1117, 1499
BccI CCATC 3 cut(s) 1009, 1144, 1381
BceAI ACGGC 3 cut(s) 121, 459, 1607
BciT130I CCWGG 2 cut(s) 1541, 1561
BclI TGATCA 2 cut(s) 811, 1345
BcoDI GTCTC 2 cut(s) 177, 1115
BfaI CTAG 3 cut(s) 227, 636, 1302
BfmI CTRYAG 1 cut(s) 1482
BfuAI ACCTGC 1 cut(s) 1319
BglII AGATCT 1 cut(s) 1486
BisI GCNGC 3 cut(s) 724, 1131, 1513
BlsI GCNGC 3 cut(s) 725, 1132, 1514
Bme1390I CCNGG 2 cut(s) 1541, 1561
Bme18I GGWCC 4 cut(s) 341, 1415, 1543, 1582
BmeRI GACNNNNNGTC 1 cut(s) 335
BmeT110I CYCGRG 2 cut(s) 41, 1065
BmgBI CACGTC 1 cut(s) 497
BmgT120I GGNCC 4 cut(s) 341, 1415, 1543, 1582
BmiI GGNNCC 4 cut(s) 101, 343, 1479, 1545
BmrFI CCNGG 2 cut(s) 1541, 1561
BmrI ACTGGG 1 cut(s) 1255
BmsI GCATC 3 cut(s) 1024, 1321, 1533
BmuI ACTGGG 1 cut(s) 1255
BpiI GAAGAC 1 cut(s) 663
BpmI CTGGAG 2 cut(s) 483, 1073
Bpu10I CCTNAGC 1 cut(s) 303
Bpu14I TTCGAA 2 cut(s) 207, 581
Bsa29I ATCGAT 1 cut(s) 1219
BsaAI YACGTR 2 cut(s) 743, 848
BsaBI GATNNNNATC 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 1506
BsaJI CCNNGG 6 cut(s) 42, 48, 103, 1320, 1411, 1559
BsaWI WCCGGW 1 cut(s) 261
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 96, 109, 1387
Bse118I RCCGGY 2 cut(s) 146, 261
Bse1I ACTGG 3 cut(s) 912, 1250, 1397
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 2 cut(s) 1541, 1561
BseCI ATCGAT 1 cut(s) 1219
BseDI CCNNGG 6 cut(s) 42, 48, 103, 1320, 1411, 1559
BseGI GGATG 4 cut(s) 511, 1015, 1042, 1524
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 4 cut(s) 65, 96, 109, 1387
BseMII CTCAG 2 cut(s) 294, 816
BseNI ACTGG 3 cut(s) 912, 1250, 1397
BseRI GAGGAG 4 cut(s) 287, 618, 668, 995
BseXI GCAGC 3 cut(s) 735, 1117, 1499
Bsh1236I CGCG 3 cut(s) 144, 167, 245
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 99
BshTI ACCGGT 1 cut(s) 261
BshVI ATCGAT 1 cut(s) 1219
BsiEI CGRYCG 1 cut(s) 179
BsiHKCI CYCGRG 2 cut(s) 41, 1065
BsiSI CCGG 2 cut(s) 147, 262
BslI CCNNNNNNNGG 4 cut(s) 65, 96, 109, 1387
BsmAI GTCTC 2 cut(s) 177, 1115
BsmI GAATGC 3 cut(s) 406, 1515, 1569
BsnI GGCC 1 cut(s) 47
BsoBI CYCGRG 2 cut(s) 41, 1065
Bsp119I TTCGAA 2 cut(s) 207, 581
Bsp143I GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 4 cut(s) 20, 96, 115, 1474
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 2 cut(s) 295, 815
BspDI ATCGAT 1 cut(s) 1219
BspFNI CGCG 3 cut(s) 144, 167, 245
BspLI GGNNCC 4 cut(s) 101, 343, 1479, 1545
BspMI ACCTGC 1 cut(s) 1319
BspPI GGATC 1 cut(s) 617
BspT104I TTCGAA 2 cut(s) 207, 581
BspT107I GGYRCC 1 cut(s) 99
BsrBI CCGCTC 1 cut(s) 115
BsrFI RCCGGY 2 cut(s) 146, 261
BsrI ACTGG 3 cut(s) 912, 1250, 1397
BssAI RCCGGY 2 cut(s) 146, 261
BssECI CCNNGG 6 cut(s) 42, 48, 103, 1320, 1411, 1559
BssMI GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
BssNI GRCGYC 1 cut(s) 1506
BssT1I CCWWGG 2 cut(s) 48, 1320
Bst2UI CCWGG 2 cut(s) 1541, 1561
Bst4CI ACNGT 4 cut(s) 91, 376, 614, 865
BstACI GRCGYC 1 cut(s) 1506
BstAPI GCANNNNNTGC 3 cut(s) 592, 1521, 1575
BstBAI YACGTR 2 cut(s) 743, 848
BstBI TTCGAA 2 cut(s) 207, 581
BstC8I GCNNGC 2 cut(s) 154, 608
BstDEI CTNAG 2 cut(s) 303, 802
BstDSI CCRYGG 2 cut(s) 48, 103
BstEII GGTNACC 1 cut(s) 54
BstF5I GGATG 4 cut(s) 511, 1015, 1042, 1524
BstFNI CGCG 3 cut(s) 144, 167, 245
BstHHI GCGC 3 cut(s) 146, 167, 406
BstKTI GATC 9 cut(s) 84, 179, 599, 625, 641, 814, 991, 1348, 1489
BstMAI GTCTC 2 cut(s) 177, 1115
BstMBI GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 5 cut(s) 162, 592, 1293, 1521, 1575
BstNI CCWGG 2 cut(s) 1541, 1561
BstNSI RCATGY 2 cut(s) 569, 1569
BstPI GGTNACC 1 cut(s) 54
BstSCI CCNGG 2 cut(s) 1539, 1559
BstSFI CTRYAG 1 cut(s) 1482
BstUI CGCG 3 cut(s) 144, 167, 245
BstV1I GCAGC 3 cut(s) 735, 1117, 1499
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 2 cut(s) 622, 1486
BstXI CCANNNNNNTGG 3 cut(s) 190, 682, 1051
BstYI RGATCY 2 cut(s) 622, 1486
Bsu15I ATCGAT 1 cut(s) 1219
BsuRI GGCC 1 cut(s) 47
BsuTUI ATCGAT 1 cut(s) 1219
BtgI CCRYGG 2 cut(s) 48, 103
BtgZI GCGATG 1 cut(s) 182
BtrI CACGTC 1 cut(s) 497
BtsCI GGATG 4 cut(s) 511, 1015, 1042, 1524
BtsI GCAGTG 2 cut(s) 1044, 1573
BtsIMutI CAGTG 6 cut(s) 381, 610, 715, 1044, 1404, 1573
BveI ACCTGC 1 cut(s) 1319
Cac8I GCNNGC 2 cut(s) 154, 608
CaiI CAGNNNCTG 4 cut(s) 461, 612, 809, 1451
CfoI GCGC 3 cut(s) 146, 167, 406
Cfr10I RCCGGY 2 cut(s) 146, 261
Cfr13I GGNCC 4 cut(s) 341, 1415, 1543, 1582
ClaI ATCGAT 1 cut(s) 1219
CseI GACGC 2 cut(s) 251, 296
Csp6I GTAC 3 cut(s) 100, 299, 843
CspAI ACCGGT 1 cut(s) 261
CviAII CATG 9 cut(s) 49, 70, 566, 727, 1161, 1209, 1233, 1339, 1566
CviQI GTAC 3 cut(s) 100, 299, 843
DdeI CTNAG 2 cut(s) 303, 802
DpnI GATC 9 cut(s) 83, 178, 598, 624, 640, 813, 990, 1347, 1488
DpnII GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
DraI TTTAAA 1 cut(s) 529
DraIII CACNNNGTG 1 cut(s) 193
DriI GACNNNNNGTC 1 cut(s) 335
Eam1105I GACNNNNNGTC 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 48, 1320
Eco47I GGWCC 4 cut(s) 341, 1415, 1543, 1582
Eco57I CTGAAG 1 cut(s) 1146
Eco88I CYCGRG 2 cut(s) 41, 1065
Eco91I GGTNACC 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 1582
EcoO65I GGTNACC 1 cut(s) 54
EcoRI GAATTC 2 cut(s) 577, 1276
EcoRII CCWGG 2 cut(s) 1539, 1559
EcoT14I CCWWGG 2 cut(s) 48, 1320
EcoT22I ATGCAT 2 cut(s) 1238, 1571
ErhI CCWWGG 2 cut(s) 48, 1320
FaeI CATG 9 cut(s) 52, 73, 569, 730, 1164, 1212, 1236, 1342, 1569
FatI CATG 9 cut(s) 48, 69, 565, 726, 1160, 1208, 1232, 1338, 1565
FauI CCCGC 3 cut(s) 27, 89, 1467
FauNDI CATATG 1 cut(s) 1257
FbaI TGATCA 2 cut(s) 811, 1345
FblI GTMKAC 1 cut(s) 778
Fnu4HI GCNGC 3 cut(s) 724, 1131, 1513
FokI GGATG 4 cut(s) 518, 1002, 1049, 1511
Fsp4HI GCNGC 3 cut(s) 724, 1131, 1513
FspBI CTAG 3 cut(s) 227, 636, 1302
GlaI GCGC 3 cut(s) 145, 166, 405
GluI GCNGC 3 cut(s) 724, 1131, 1513
GsuI CTGGAG 2 cut(s) 483, 1073
HaeIII GGCC 1 cut(s) 47
HapII CCGG 2 cut(s) 147, 262
HgaI GACGC 2 cut(s) 251, 296
HhaI GCGC 3 cut(s) 146, 167, 406
Hin1I GRCGYC 1 cut(s) 1506
Hin1II CATG 9 cut(s) 52, 73, 569, 730, 1164, 1212, 1236, 1342, 1569
Hin6I GCGC 3 cut(s) 144, 165, 404
HinP1I GCGC 3 cut(s) 144, 165, 404
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 755
HpaII CCGG 2 cut(s) 147, 262
HphI GGTGA 4 cut(s) 48, 392, 697, 989
Hpy166II GTNNAC 4 cut(s) 256, 329, 779, 845
Hpy188III TCNNGA 7 cut(s) 41, 174, 462, 617, 665, 815, 1052
Hpy8I GTNNAC 4 cut(s) 256, 329, 779, 845
Hpy99I CGWCG 2 cut(s) 245, 1593
HpyAV CCTTC 8 cut(s) 203, 207, 567, 642, 652, 1103, 1155, 1610
HpyCH4III ACNGT 4 cut(s) 91, 376, 614, 865
HpyCH4IV ACGT 5 cut(s) 496, 742, 781, 847, 1506
HpyCH4V TGCA 9 cut(s) 279, 1236, 1296, 1314, 1334, 1515, 1524, 1569, 1578
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 592, 1293, 1521, 1575
HpyF3I CTNAG 2 cut(s) 303, 802
HpySE526I ACGT 5 cut(s) 496, 742, 781, 847, 1506
Hsp92I GRCGYC 1 cut(s) 1506
Hsp92II CATG 9 cut(s) 52, 73, 569, 730, 1164, 1212, 1236, 1342, 1569
HspAI GCGC 3 cut(s) 144, 165, 404
KpnI GGTACC 1 cut(s) 103
Ksp22I TGATCA 2 cut(s) 811, 1345
Kzo9I GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
LmnI GCTCC 3 cut(s) 112, 464, 1438
Lsp1109I GCAGC 3 cut(s) 735, 1117, 1499
LweI GCATC 3 cut(s) 1024, 1321, 1533
MaeI CTAG 3 cut(s) 227, 636, 1302
MaeII ACGT 5 cut(s) 496, 742, 781, 847, 1506
MaeIII GTNAC 3 cut(s) 54, 370, 1502
MalI GATC 9 cut(s) 83, 178, 598, 624, 640, 813, 990, 1347, 1488
MbiI CCGCTC 1 cut(s) 115
MboI GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
MboII GAAGA 8 cut(s) 632, 663, 899, 960, 963, 996, 999, 1481
MflI RGATCY 2 cut(s) 622, 1486
MluCI AATT 9 cut(s) 203, 444, 511, 577, 1140, 1191, 1200, 1276, 1423
MlyI GAGTC 4 cut(s) 428, 467, 677, 815
MmeI TCCRAC 4 cut(s) 179, 319, 550, 1612
Mph1103I ATGCAT 2 cut(s) 1238, 1571
MroXI GAANNNNTTC 2 cut(s) 577, 1276
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 1397
MspA1I CMGCKG 1 cut(s) 1474
MspI CCGG 2 cut(s) 147, 262
MspR9I CCNGG 2 cut(s) 1541, 1561
Mva1269I GAATGC 3 cut(s) 406, 1515, 1569
MvaI CCWGG 2 cut(s) 1541, 1561
MvnI CGCG 3 cut(s) 144, 167, 245
MwoI GCNNNNNNNGC 5 cut(s) 162, 592, 1293, 1521, 1575
NcoI CCATGG 1 cut(s) 48
NdeI CATATG 1 cut(s) 1257
NdeII GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
NlaIII CATG 9 cut(s) 52, 73, 569, 730, 1164, 1212, 1236, 1342, 1569
NlaIV GGNNCC 4 cut(s) 101, 343, 1479, 1545
NmuCI GTSAC 2 cut(s) 54, 1502
NsiI ATGCAT 2 cut(s) 1238, 1571
NspI RCATGY 2 cut(s) 569, 1569
NspV TTCGAA 2 cut(s) 207, 581
PciI ACATGT 1 cut(s) 565
PctI GAATGC 3 cut(s) 406, 1515, 1569
PdmI GAANNNNTTC 2 cut(s) 577, 1276
PinAI ACCGGT 1 cut(s) 261
PkrI GCNGC 3 cut(s) 725, 1132, 1514
Ple19I CGATCG 1 cut(s) 179
PleI GAGTC 4 cut(s) 428, 466, 676, 814
PpsI GAGTC 4 cut(s) 428, 466, 676, 814
Ppu21I YACGTR 2 cut(s) 743, 848
PpuMI RGGWCCY 1 cut(s) 1582
PscI ACATGT 1 cut(s) 565
PsiI TTATAA 1 cut(s) 761
Psp5II RGGWCCY 1 cut(s) 1582
Psp6I CCWGG 2 cut(s) 1539, 1559
PspEI GGTNACC 1 cut(s) 54
PspGI CCWGG 2 cut(s) 1539, 1559
PspN4I GGNNCC 4 cut(s) 101, 343, 1479, 1545
PspPI GGNCC 4 cut(s) 341, 1415, 1543, 1582
PspPPI RGGWCCY 1 cut(s) 1582
PstNI CAGNNNCTG 4 cut(s) 461, 612, 809, 1451
PsuI RGATCY 2 cut(s) 622, 1486
PvuI CGATCG 1 cut(s) 179
RsaI GTAC 3 cut(s) 101, 300, 844
RsaNI GTAC 3 cut(s) 100, 299, 843
RseI CAYNNNNRTG 1 cut(s) 1397
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 3 cut(s) 724, 1131, 1513
Sau3AI GATC 9 cut(s) 81, 176, 596, 622, 638, 811, 988, 1345, 1486
Sau96I GGNCC 4 cut(s) 341, 1415, 1543, 1582
SchI GAGTC 4 cut(s) 428, 467, 677, 815
ScrFI CCNGG 2 cut(s) 1541, 1561
SfaNI GCATC 3 cut(s) 1024, 1321, 1533
SfcI CTRYAG 1 cut(s) 1482
SfuI TTCGAA 2 cut(s) 207, 581
SgrAI CRCCGGYG 2 cut(s) 146, 261
SinI GGWCC 4 cut(s) 341, 1415, 1543, 1582
SmiMI CAYNNNNRTG 1 cut(s) 1397
Sse9I AATT 9 cut(s) 203, 444, 511, 577, 1140, 1191, 1200, 1276, 1423
SsiI CCGC 4 cut(s) 20, 96, 115, 1474
SspI AATATT 2 cut(s) 772, 1024
SspMI CTAG 3 cut(s) 227, 636, 1302
StyD4I CCNGG 2 cut(s) 1539, 1559
StyI CCWWGG 2 cut(s) 48, 1320
TaaI ACNGT 4 cut(s) 91, 376, 614, 865
TaiI ACGT 5 cut(s) 499, 745, 784, 850, 1509
TaqI TCGA 9 cut(s) 175, 179, 207, 240, 389, 581, 1148, 1195, 1219
TasI AATT 9 cut(s) 203, 444, 511, 577, 1140, 1191, 1200, 1276, 1423
TatI WGTACW 1 cut(s) 842
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 6 cut(s) 381, 617, 715, 1051, 1404, 1580
TseFI GTSAC 2 cut(s) 54, 1502
TseI GCWGC 3 cut(s) 723, 1130, 1512
Tsp45I GTSAC 2 cut(s) 54, 1502
TspDTI ATGAA 4 cut(s) 900, 1053, 1086, 1221
TspGWI ACGGA 2 cut(s) 67, 1425
TspRI CASTG 6 cut(s) 381, 617, 715, 1051, 1404, 1580
VpaK11BI GGWCC 4 cut(s) 341, 1415, 1543, 1582
XapI RAATTY 6 cut(s) 203, 511, 577, 1140, 1200, 1276
XceI RCATGY 2 cut(s) 569, 1569
XmiI GTMKAC 1 cut(s) 778
XmnI GAANNNNTTC 2 cut(s) 577, 1276
XspI CTAG 3 cut(s) 227, 636, 1302
ZraI GACGTC 1 cut(s) 1507
Zsp2I ATGCAT 2 cut(s) 1238, 1571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.