RLG00000021417

twin BRCT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004814
Physical Location & Seq
Reverse (-)
75237110 .. 75241474
4365 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021417

Sequence Viewer

Length: 1518 bp
ATGGCATCTGATCCAATTCCAGGCATGGAGTCGGTGGTGGCCTCCGTCAGTGGGTACCGGGGATCGGAGCTGGTGAATCTGATCGAGCTCATGACTCACACCGGTGCCAGCTATATCGGTACCTTGTCGCCGTCCAATGCCTTCACTCATATGGTGTGCCGGAGATTTGAGGGAAAGAGATATCAAGTGGCTAAAAAGTTGAAACTCATAATTGTTAACCACCGGTGGATTGAGGATTGCGTAAAGCATGGAAAGCGAGTTCCTGAGCATCCCTATACCTTGCAAAGTGGACAGGAAGTGGGACCCTTACTGTTGAAAGTTCCACTTATTGCTAAACCAGGCTTCTCGGTTAAGAAGGGGAAAGCGGTTTCTGATAAATCCCATGTTTTTGGCGACTCTGAAGAAATTATCGATCTGGGGTGTGGATCTTCTCGCCGTGGTGTTTTGACTGATTCACGTCTATTGAATGAGAATTCGTTTCCTGACTTCAAAGAAAACAATAGTTCCCAGAAGCAACAGAATAAATCTGTGAAAACAAATTCAAAGAAAGAGCCGCAATCTAGTAGCAGAGACTGTTTTGAAGATCCTCCTTTATCCAGATCACTCAGAATGGAGGAGTTTTGCAGGCATTCAGTTAGAGCTCAAAGAAATATTACCAGTGACACTGGAACCAGCATGACTGAAATTCCATGTAAAAAAAGGAGGCTTGTGAAAAAGAATATTGGCTCTACAATATCAGTACTCTCAGAATCTGATCGAGAATGCCACCCAATCAAATTTCACAGTATCCGTAATGACAGGGTCACAATCCTTTCTGATGATTCTAATGACGAGTTAGACACTGGAACAGGAATCAGAGATGTGGGACGTAAAGGTGTTGATGTTGGTGGTAATCACTTATCTACTTACAAGAATCCCAATTTATCTGTTGTGGATGAGCCTACAGCTCCTGAGGGAACTCCACAAAATGGGTGCCCTGAAGTAGAGAATTCAAAGGAGGAATTGGAAATCAGGTTGGACAGAGAACATGCCACCAAAAGTCCTTCACAGGAATTATCATGTGTTATATGCTGGACAGAGTTTAGTACAACCCGAGGAATTTTGCCATGTGGTCATCGATACTGTTATTCTTGTATTGAAAATTGGGCTGATCAACTGGGTTCAAGGAGAAAGATTTCAACATGCCCTTTATGCAAAGCTTCGTTTACAGTCATTACCAAGGTTGATGATGCAGTCACTAGTGATCAAAATATATTTTCTCAAACCATTCCATCTGCCCCAAAAGTGGACATTTCATTTCTTAATGACCAGGGAACATCTAATTTTGCTGCTCAGTCCGTATCAGCAAATGGTTGTTCTGTGTGTAATTCTTGGGAACCCGAAGAGCTGCTTATTAGTTGTAATGTCTGCCAAATTCAGCGCATCCATACCTACTGCCAGGACCCTTTTTCAACTACATGGACATGCATTCACTGCAAAGATCTTCGAATGCTTTACCGCAGCTATCATCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00590 ko04918 map00480 map00590 map04918
Pfam Domains
Protein Families
PTCB-BRCT zf-C3HC4 zf-RING_2

Protein Analysis

506

Amino Acids

56.34

Weight (kDa)

7.75

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTCB-BRCT PF12738 12 - 76 1.2e-15 twin BRCT domain
zf-C3HC4_3 PF13920 351 - 402 1.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 353 - 399 6.6e-09 Ring finger domain
zf-C3HC4 PF00097 354 - 398 6.2e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 354 - 396 5.8e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 54, 119
AccB1I GGYRCC 4 cut(s) 54, 104, 119, 972
AccB7I CCANNNNNTGG 1 cut(s) 968
AciI CCGC 3 cut(s) 365, 554, 1498
AclWI GGATC 4 cut(s) 5, 70, 433, 578
AcsI RAATTY 7 cut(s) 472, 538, 684, 776, 988, 1098, 1413
AcuI CTGAAG 2 cut(s) 420, 999
AfaI GTAC 4 cut(s) 56, 121, 741, 1087
AfiI CCNNNNNNNGG 5 cut(s) 20, 51, 64, 968, 1285
AgeI ACCGGT 2 cut(s) 101, 222
AhlI ACTAGT 1 cut(s) 1238
AjiI CACGTC 1 cut(s) 458
AjnI CCWGG 4 cut(s) 19, 337, 1308, 1437
AleI CACNNNNGTG 1 cut(s) 102
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 8 cut(s) 70, 88, 111, 641, 947, 1199, 1387, 1503
AluI AGCT 8 cut(s) 70, 88, 111, 641, 947, 1199, 1387, 1503
Alw21I GWGCWC 2 cut(s) 90, 643
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 4 cut(s) 5, 70, 433, 578
AlwNI CAGNNNCTG 3 cut(s) 573, 752, 950
Ama87I CYCGRG 1 cut(s) 1092
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 3 cut(s) 1328, 1387, 1500
ApoI RAATTY 7 cut(s) 472, 538, 684, 776, 988, 1098, 1413
AsiGI ACCGGT 2 cut(s) 101, 222
Asp700I GAANNNNTTC 1 cut(s) 1174
Asp718I GGTACC 2 cut(s) 54, 119
AspLEI GCGC 1 cut(s) 1422
AspS9I GGNCC 2 cut(s) 302, 1441
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 85
AsuII TTCGAA 1 cut(s) 1486
AvaI CYCGRG 1 cut(s) 1092
AvaII GGWCC 2 cut(s) 302, 1441
AxyI CCTNAGG 1 cut(s) 951
BaeGI GKGCMC 1 cut(s) 977
BanI GGYRCC 4 cut(s) 54, 104, 119, 972
BanII GRGCYC 2 cut(s) 90, 643
Bbv12I GWGCWC 2 cut(s) 90, 643
BbvI GCAGC 3 cut(s) 1315, 1374, 1512
BccI CCATC 1 cut(s) 1279
BceAI ACGGC 2 cut(s) 115, 420
BciT130I CCWGG 4 cut(s) 21, 339, 1310, 1439
BciVI GTATCC 1 cut(s) 797
BclI TGATCA 2 cut(s) 1150, 1243
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 564
BcuI ACTAGT 1 cut(s) 1238
BfaI CTAG 2 cut(s) 561, 1239
BfmI CTRYAG 1 cut(s) 942
BfuI GTATCC 1 cut(s) 797
BglII AGATCT 1 cut(s) 1480
BisI GCNGC 4 cut(s) 554, 1329, 1388, 1501
BlsI GCNGC 4 cut(s) 555, 1330, 1389, 1502
BmcAI AGTACT 1 cut(s) 741
Bme1390I CCNGG 5 cut(s) 21, 59, 339, 1310, 1439
Bme18I GGWCC 2 cut(s) 302, 1441
BmeT110I CYCGRG 1 cut(s) 1092
BmgBI CACGTC 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 302, 1441
BmiI GGNNCC 9 cut(s) 56, 106, 121, 303, 304, 670, 974, 1377, 1443
BmrFI CCNGG 5 cut(s) 21, 59, 339, 1310, 1439
BmrI ACTGGG 1 cut(s) 1166
BmsI GCATC 4 cut(s) 14, 277, 1219, 1431
BmuI ACTGGG 1 cut(s) 1166
Bpu10I CCTNAGC 1 cut(s) 264
Bpu14I TTCGAA 1 cut(s) 1486
BpuMI CCSGG 1 cut(s) 59
Bsa29I ATCGAT 2 cut(s) 411, 1117
BsaJI CCNNGG 5 cut(s) 58, 436, 1093, 1218, 1309
BsaWI WCCGGW 2 cut(s) 101, 222
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 51, 64, 968, 1285
Bse118I RCCGGY 2 cut(s) 101, 222
Bse1I ACTGG 4 cut(s) 657, 670, 847, 1161
Bse21I CCTNAGG 1 cut(s) 951
BseBI CCWGG 4 cut(s) 21, 339, 1310, 1439
BseCI ATCGAT 2 cut(s) 411, 1117
BseDI CCNNGG 5 cut(s) 58, 436, 1093, 1218, 1309
BseGI GGATG 3 cut(s) 268, 940, 1422
BseLI CCNNNNNNNGG 5 cut(s) 20, 51, 64, 968, 1285
BseMII CTCAG 5 cut(s) 255, 619, 759, 942, 1346
BseNI ACTGG 4 cut(s) 657, 670, 847, 1161
BseRI GAGGAG 1 cut(s) 629
BseSI GKGCMC 1 cut(s) 977
BseXI GCAGC 3 cut(s) 1315, 1374, 1512
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 4 cut(s) 54, 104, 119, 972
BshTI ACCGGT 2 cut(s) 101, 222
BshVI ATCGAT 2 cut(s) 411, 1117
BsiHKAI GWGCWC 2 cut(s) 90, 643
BsiHKCI CYCGRG 1 cut(s) 1092
BsiSI CCGG 4 cut(s) 58, 102, 160, 223
BslFI GGGAC 2 cut(s) 315, 879
BslI CCNNNNNNNGG 5 cut(s) 20, 51, 64, 968, 1285
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 2 cut(s) 315, 879
BsmI GAATGC 4 cut(s) 628, 767, 1467, 1494
BsnI GGCC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 1092
Bsp119I TTCGAA 1 cut(s) 1486
Bsp1286I GDGCHC 3 cut(s) 90, 643, 977
BspACI CCGC 3 cut(s) 365, 554, 1498
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 5 cut(s) 256, 618, 758, 943, 1345
BspDI ATCGAT 2 cut(s) 411, 1117
BspHI TCATGA 1 cut(s) 90
BspLI GGNNCC 9 cut(s) 56, 106, 121, 303, 304, 670, 974, 1377, 1443
BspPI GGATC 4 cut(s) 5, 70, 433, 578
BspQI GCTCTTC 1 cut(s) 1377
BspT104I TTCGAA 1 cut(s) 1486
BspT107I GGYRCC 4 cut(s) 54, 104, 119, 972
BsrFI RCCGGY 2 cut(s) 101, 222
BsrI ACTGG 4 cut(s) 657, 670, 847, 1161
BssAI RCCGGY 2 cut(s) 101, 222
BssECI CCNNGG 5 cut(s) 58, 436, 1093, 1218, 1309
BssT1I CCWWGG 1 cut(s) 1218
Bst2UI CCWGG 4 cut(s) 21, 339, 1310, 1439
Bst4CI ACNGT 5 cut(s) 312, 575, 785, 1124, 1210
Bst6I CTCTTC 1 cut(s) 1377
BstAPI GCANNNNNTGC 1 cut(s) 1473
BstBI TTCGAA 1 cut(s) 1486
BstC8I GCNNGC 2 cut(s) 109, 626
BstDEI CTNAG 5 cut(s) 264, 605, 745, 951, 1332
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 3 cut(s) 268, 940, 1422
BstHHI GCGC 1 cut(s) 1422
BstMAI GTCTC 1 cut(s) 564
BstMWI GCNNNNNNNGC 3 cut(s) 253, 1191, 1473
BstNI CCWGG 4 cut(s) 21, 339, 1310, 1439
BstNSI RCATGY 3 cut(s) 1031, 1185, 1467
BstSCI CCNGG 5 cut(s) 19, 57, 337, 1308, 1437
BstSFI CTRYAG 1 cut(s) 942
BstSLI GKGCMC 1 cut(s) 977
BstV1I GCAGC 3 cut(s) 1315, 1374, 1512
BstX2I RGATCY 3 cut(s) 425, 583, 1480
BstXI CCANNNNNNTGG 1 cut(s) 389
BstYI RGATCY 3 cut(s) 425, 583, 1480
Bsu15I ATCGAT 2 cut(s) 411, 1117
Bsu36I CCTNAGG 1 cut(s) 951
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 1 cut(s) 41
BsuTUI ATCGAT 2 cut(s) 411, 1117
BtgI CCRYGG 1 cut(s) 436
BtrI CACGTC 1 cut(s) 458
BtsCI GGATG 3 cut(s) 268, 940, 1422
BtsI GCAGTG 1 cut(s) 1471
BtsIMutI CAGTG 5 cut(s) 55, 663, 664, 840, 1471
Cac8I GCNNGC 2 cut(s) 109, 626
CaiI CAGNNNCTG 3 cut(s) 573, 752, 950
CciI TCATGA 1 cut(s) 90
CfoI GCGC 1 cut(s) 1422
Cfr10I RCCGGY 2 cut(s) 101, 222
Cfr13I GGNCC 2 cut(s) 302, 1441
ClaI ATCGAT 2 cut(s) 411, 1117
Csp6I GTAC 4 cut(s) 55, 120, 740, 1086
CspAI ACCGGT 2 cut(s) 101, 222
CviQI GTAC 4 cut(s) 55, 120, 740, 1086
DdeI CTNAG 5 cut(s) 264, 605, 745, 951, 1332
Eam1104I CTCTTC 1 cut(s) 1377
EarI CTCTTC 1 cut(s) 1377
Ecl136II GAGCTC 2 cut(s) 88, 641
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 2 cut(s) 90, 643
Eco32I GATATC 1 cut(s) 182
Eco47I GGWCC 2 cut(s) 302, 1441
Eco53kI GAGCTC 2 cut(s) 88, 641
Eco57I CTGAAG 2 cut(s) 420, 999
Eco81I CCTNAGG 1 cut(s) 951
Eco88I CYCGRG 1 cut(s) 1092
EcoICRI GAGCTC 2 cut(s) 88, 641
EcoO109I RGGNCCY 2 cut(s) 302, 1441
EcoRI GAATTC 2 cut(s) 472, 988
EcoRII CCWGG 4 cut(s) 19, 337, 1308, 1437
EcoRV GATATC 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 1218
EcoT22I ATGCAT 1 cut(s) 1469
EcoT38I GRGCYC 2 cut(s) 90, 643
ErhI CCWWGG 1 cut(s) 1218
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 1374, 1406
FaqI GGGAC 2 cut(s) 315, 879
FauNDI CATATG 1 cut(s) 150
FbaI TGATCA 2 cut(s) 1150, 1243
Fnu4HI GCNGC 4 cut(s) 554, 1329, 1388, 1501
FokI GGATG 3 cut(s) 255, 947, 1409
FriOI GRGCYC 2 cut(s) 90, 643
Fsp4HI GCNGC 4 cut(s) 554, 1329, 1388, 1501
FspBI CTAG 2 cut(s) 561, 1239
GlaI GCGC 1 cut(s) 1421
GluI GCNGC 4 cut(s) 554, 1329, 1388, 1501
HaeIII GGCC 1 cut(s) 41
HapII CCGG 4 cut(s) 58, 102, 160, 223
HhaI GCGC 1 cut(s) 1422
Hin6I GCGC 1 cut(s) 1420
HinP1I GCGC 1 cut(s) 1420
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 1197
HinfI GANTC 9 cut(s) 29, 76, 94, 395, 452, 749, 821, 852, 913
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 4 cut(s) 58, 102, 160, 223
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 4 cut(s) 217, 290, 1206, 1288
Hpy188III TCNNGA 6 cut(s) 91, 263, 482, 597, 758, 950
Hpy8I GTNNAC 4 cut(s) 217, 290, 1206, 1288
HpyAV CCTTC 3 cut(s) 151, 349, 1053
HpyCH4III ACNGT 5 cut(s) 312, 575, 785, 1124, 1210
HpyCH4IV ACGT 2 cut(s) 457, 868
HpyCH4V TGCA 6 cut(s) 283, 624, 1194, 1232, 1467, 1476
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 1191, 1473
HpyF3I CTNAG 5 cut(s) 264, 605, 745, 951, 1332
HpySE526I ACGT 2 cut(s) 457, 868
HspAI GCGC 1 cut(s) 1420
KflI GGGWCCC 1 cut(s) 302
KpnI GGTACC 2 cut(s) 58, 123
Ksp22I TGATCA 2 cut(s) 1150, 1243
KspAI GTTAAC 1 cut(s) 217
LguI GCTCTTC 1 cut(s) 1377
LmnI GCTCC 2 cut(s) 67, 952
Lsp1109I GCAGC 3 cut(s) 1315, 1374, 1512
LweI GCATC 4 cut(s) 14, 277, 1219, 1431
MaeI CTAG 2 cut(s) 561, 1239
MaeII ACGT 2 cut(s) 457, 868
MaeIII GTNAC 3 cut(s) 659, 802, 1234
MboII GAAGA 5 cut(s) 413, 420, 593, 1394, 1475
MflI RGATCY 3 cut(s) 425, 583, 1480
MhlI GDGCHC 3 cut(s) 90, 643, 977
MlyI GAGTC 3 cut(s) 38, 88, 389
MmeI TCCRAC 1 cut(s) 996
MnlI CCTC 9 cut(s) 52, 163, 226, 597, 607, 696, 946, 991, 1088
Mph1103I ATGCAT 1 cut(s) 1469
MroXI GAANNNNTTC 1 cut(s) 1174
MseI TTAA 3 cut(s) 216, 351, 1302
MslI CAYNNNNRTG 3 cut(s) 102, 149, 1462
MspI CCGG 4 cut(s) 58, 102, 160, 223
MspR9I CCNGG 5 cut(s) 21, 59, 339, 1310, 1439
Mva1269I GAATGC 4 cut(s) 628, 767, 1467, 1494
MvaI CCWGG 4 cut(s) 21, 339, 1310, 1439
MwoI GCNNNNNNNGC 3 cut(s) 253, 1191, 1473
NciI CCSGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 150
NlaIV GGNNCC 9 cut(s) 56, 106, 121, 303, 304, 670, 974, 1377, 1443
NmuCI GTSAC 3 cut(s) 659, 802, 1234
NsiI ATGCAT 1 cut(s) 1469
NspI RCATGY 3 cut(s) 1031, 1185, 1467
NspV TTCGAA 1 cut(s) 1486
OliI CACNNNNGTG 1 cut(s) 102
PagI TCATGA 1 cut(s) 90
PciSI GCTCTTC 1 cut(s) 1377
PctI GAATGC 4 cut(s) 628, 767, 1467, 1494
PdmI GAANNNNTTC 1 cut(s) 1174
PfeI GAWTC 6 cut(s) 76, 452, 749, 821, 852, 913
PflFI GACNNNGTC 1 cut(s) 800
PflMI CCANNNNNTGG 1 cut(s) 968
PinAI ACCGGT 2 cut(s) 101, 222
PkrI GCNGC 4 cut(s) 555, 1330, 1389, 1502
PleI GAGTC 3 cut(s) 37, 88, 389
PpsI GAGTC 3 cut(s) 37, 88, 389
PpuMI RGGWCCY 2 cut(s) 302, 1441
Psp124BI GAGCTC 2 cut(s) 90, 643
Psp5II RGGWCCY 2 cut(s) 302, 1441
Psp6I CCWGG 4 cut(s) 19, 337, 1308, 1437
PspGI CCWGG 4 cut(s) 19, 337, 1308, 1437
PspN4I GGNNCC 9 cut(s) 56, 106, 121, 303, 304, 670, 974, 1377, 1443
PspPI GGNCC 2 cut(s) 302, 1441
PspPPI RGGWCCY 2 cut(s) 302, 1441
PstNI CAGNNNCTG 3 cut(s) 573, 752, 950
PsuI RGATCY 3 cut(s) 425, 583, 1480
PsyI GACNNNGTC 1 cut(s) 800
RsaI GTAC 4 cut(s) 56, 121, 741, 1087
RsaNI GTAC 4 cut(s) 55, 120, 740, 1086
RseI CAYNNNNRTG 3 cut(s) 102, 149, 1462
SacI GAGCTC 2 cut(s) 90, 643
SapI GCTCTTC 1 cut(s) 1377
SaqAI TTAA 3 cut(s) 216, 351, 1302
SatI GCNGC 4 cut(s) 554, 1329, 1388, 1501
Sau96I GGNCC 2 cut(s) 302, 1441
ScaI AGTACT 1 cut(s) 741
SchI GAGTC 3 cut(s) 38, 88, 389
ScrFI CCNGG 5 cut(s) 21, 59, 339, 1310, 1439
SduI GDGCHC 3 cut(s) 90, 643, 977
SfaNI GCATC 4 cut(s) 14, 277, 1219, 1431
SfcI CTRYAG 1 cut(s) 942
SfuI TTCGAA 1 cut(s) 1486
SgrAI CRCCGGYG 2 cut(s) 101, 222
SinI GGWCC 2 cut(s) 302, 1441
SmiMI CAYNNNNRTG 3 cut(s) 102, 149, 1462
SpeI ACTAGT 1 cut(s) 1238
SsiI CCGC 3 cut(s) 365, 554, 1498
SspI AATATT 3 cut(s) 652, 721, 1514
SspMI CTAG 2 cut(s) 561, 1239
SstI GAGCTC 2 cut(s) 90, 643
StyD4I CCNGG 5 cut(s) 19, 57, 337, 1308, 1437
StyI CCWWGG 1 cut(s) 1218
TaaI ACNGT 5 cut(s) 312, 575, 785, 1124, 1210
TaiI ACGT 2 cut(s) 460, 871
TaqI TCGA 5 cut(s) 84, 411, 757, 1117, 1486
TatI WGTACW 2 cut(s) 739, 1085
TauI GCSGC 1 cut(s) 556
TfiI GAWTC 6 cut(s) 76, 452, 749, 821, 852, 913
Tru1I TTAA 3 cut(s) 216, 351, 1302
Tru9I TTAA 3 cut(s) 216, 351, 1302
TscAI CASTG 5 cut(s) 55, 664, 670, 847, 1478
TseFI GTSAC 3 cut(s) 659, 802, 1234
TseI GCWGC 3 cut(s) 1328, 1387, 1500
Tsp45I GTSAC 3 cut(s) 659, 802, 1234
TspDTI ATGAA 1 cut(s) 1284
TspGWI ACGGA 3 cut(s) 34, 779, 1327
TspRI CASTG 5 cut(s) 55, 664, 670, 847, 1478
Tth111I GACNNNGTC 1 cut(s) 800
Van91I CCANNNNNTGG 1 cut(s) 968
VpaK11BI GGWCC 2 cut(s) 302, 1441
XapI RAATTY 7 cut(s) 472, 538, 684, 776, 988, 1098, 1413
XceI RCATGY 3 cut(s) 1031, 1185, 1467
XmnI GAANNNNTTC 1 cut(s) 1174
XspI CTAG 2 cut(s) 561, 1239
ZrmI AGTACT 1 cut(s) 741
Zsp2I ATGCAT 1 cut(s) 1469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.