FvH4_6g47542

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0020342
Physical Location & Seq
Forward (+)
35999764 .. 36001529
1766 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47542.t1

Sequence Viewer

Length: 1428 bp
ATGGCTTTGCGTTTTTCCCGTGAAGAGAAGGGAAAGTTCTTATCGAAGAGTACTCCTAGTGAGTATTTTTCTCCTCAAGGGAAGGAAAAGTTCTCAATCATGAAGAGTCATGAGTTTGAAGAAGTGGCAAGAGCAATGAGTGAAGATGTGTCAAATACTCCCTATGGTTATGTCTATAAGCCTCTCTGGGATTCCTTATGGAATGTCCGCGTCCCCGGTAGGGTGAAGATCTGTGCTTGGAAGGCTTCTATGGACATTCTCCCCACTCGTGCTCATCTACACCAAAAGGGTATGTCTCTAAAACATCGAGGCTGTATGTTTTGCGACGACAATTCTCTAGAATCATCGCTGCATGTCTTGAAGGATTGCCCTTATGCAACTTCTGTGTGGTTCTCTAGTGCTTTGGCTATACGCACTAGCGAGTTGCAGTGTATCACCGTTAAGGATTGGTTGTTACATCTCTCTATAGCCTTACCAGCTTCCCAGTTTGCAATGCTGATGATGACTCTTTGGTCAATCTGGAATGAGCGTCATTCTCTTCTGTTAGGGAAATGTAAGAAGCTTTCACCCCCTGATGATGTTGCTCGTCGTGCTCAAACTTGGCTACAAGAGTTCATGACACTTCCACAAAATCTGAGCCCTAAACCTCTACAACCTAATCAAAAGTGGTCGAGGCCAAACGAGGGTTGGGTGAAGGTCAATGTGGACGGGGTTTATGATCGTGCTGGCTCTAGTGGAGGGATCGGAGTGATTCTTCGTGACTGGAATGGTGATTTCCATGGCGCATTAACTAAGAAAATATATGGTGGACTGTTTTCACAAGTAGATGTAGAGGTTGTGGCATTGCGGGAAGGCGTTCGCTTTGCTCTCGACCGTGGCTATCACCAGGTATGGTTCGAAAGTGACGCATTGAACACTATAATTGCTCGCTACAATATAGAGTATCATCGAGAAGAGGACGATGAAGATGTTTATATAAGTGATAGCAGTAGTGACAGTGGAAGTGATAGTGACACCGAAAATGATAGTGATGGTGACACAGCCATGCCGGCAAGTTTTACGCCTGTTAGTCGAGTAGCAAGGATGGTGCTGGATAATCTTCGTGGCCCGAAAAGTAGCTTATTACCAAGTATTGATCATGTTCATCATCATCCTGCTGGTTCCGGTGATTCTGATACATCTTTATATGTCGGTTTGTTTAGCTCGATTGATCCTGTGATTGAAGACACAAGGGCAATGCTGCGTGGTTTTCAGGGGGAACCAGGCTTAACTCATATTCATCCGAGTGCAAATTCTGTTGCGCGTAGATTGGCTACGCACGCCTTGTGTATGAAACAACGTGACTATTGTTGCTGGTTTAAAGAGCCCCCGAACTTCATCTTGGATGTCCTGCTTGAAGACTGTAACATTTCTCCGACCCTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

476

Amino Acids

53.57

Weight (kDa)

6.11

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 61 - 130 2.3e-11 zinc-binding in reverse transcriptase
RVT_3 PF13456 234 - 304 1.8e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020342)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g47542
pyrus_communis pycom03g14340
rosa_laevigata RLG00000021637
rosa_roxburghii Rroxscaffold_2G00084690
rosa_samantha Rh2AG590700 Rh2DG613500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 511
AccII CGCG 2 cut(s) 210, 1303
AciI CCGC 2 cut(s) 208, 847
AclWI GGATC 2 cut(s) 749, 1205
AcsI RAATTY 1 cut(s) 1291
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 220, 683
AgsI TTSAA 5 cut(s) 119, 361, 913, 1223, 1397
AjnI CCWGG 2 cut(s) 885, 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 1364, 1396
AluBI AGCT 4 cut(s) 479, 562, 1119, 1203
AluI AGCT 4 cut(s) 479, 562, 1119, 1203
Alw21I GWGCWC 2 cut(s) 274, 595
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 2 cut(s) 749, 1205
AoxI GGCC 2 cut(s) 674, 1105
ApeKI GCWGC 2 cut(s) 349, 1240
ApoI RAATTY 1 cut(s) 1291
Asp700I GAANNNNTTC 1 cut(s) 855
AspLEI GCGC 2 cut(s) 785, 1303
AspS9I GGNCC 1 cut(s) 1106
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 8 cut(s) 235, 427, 558, 703, 782, 875, 1046, 1178
AsuII TTCGAA 1 cut(s) 897
BanII GRGCYC 2 cut(s) 641, 1368
BauI CACGAG 1 cut(s) 267
BbsI GAAGAC 2 cut(s) 1230, 1404
Bbv12I GWGCWC 2 cut(s) 274, 595
BbvI GCAGC 2 cut(s) 336, 1227
BccI CCATC 2 cut(s) 1025, 1078
BciT130I CCWGG 2 cut(s) 887, 1263
BclI TGATCA 1 cut(s) 1135
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 300
BfaI CTAG 5 cut(s) 57, 338, 396, 417, 732
BfmI CTRYAG 1 cut(s) 465
BglI GCCNNNNNGGC 1 cut(s) 1049
BglII AGATCT 1 cut(s) 228
BisI GCNGC 2 cut(s) 350, 1241
BlsI GCNGC 2 cut(s) 351, 1242
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 3 cut(s) 216, 887, 1263
BmgT120I GGNCC 1 cut(s) 1106
BmiI GGNNCC 2 cut(s) 1162, 1260
BmrFI CCNGG 3 cut(s) 216, 887, 1263
BmrI ACTGGG 1 cut(s) 478
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 2 cut(s) 1230, 1404
Bpu14I TTCGAA 1 cut(s) 897
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 214, 778, 874
BsaWI WCCGGW 1 cut(s) 1163
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 683
Bse118I RCCGGY 1 cut(s) 1048
Bse1I ACTGG 2 cut(s) 484, 767
Bse3DI GCAATG 4 cut(s) 141, 498, 842, 1242
BseBI CCWGG 2 cut(s) 887, 1263
BseDI CCNNGG 3 cut(s) 214, 778, 874
BseGI GGATG 4 cut(s) 1089, 1150, 1279, 1390
BseLI CCNNNNNNNGG 2 cut(s) 220, 683
BseMI GCAATG 4 cut(s) 141, 498, 842, 1242
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 2 cut(s) 484, 767
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 2 cut(s) 336, 1227
Bsh1236I CGCG 2 cut(s) 210, 1303
Bsh1285I CGRYCG 1 cut(s) 874
BshFI GGCC 2 cut(s) 676, 1107
BsiEI CGRYCG 1 cut(s) 874
BsiHKAI GWGCWC 2 cut(s) 274, 595
BsiSI CCGG 3 cut(s) 216, 1049, 1164
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 220, 683
BsmAI GTCTC 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 2 cut(s) 676, 1107
Bsp119I TTCGAA 1 cut(s) 897
Bsp1286I GDGCHC 4 cut(s) 274, 595, 641, 1368
Bsp143I GATC 5 cut(s) 228, 718, 741, 1135, 1210
Bsp19I CCATGG 1 cut(s) 778
BspACI CCGC 2 cut(s) 208, 847
BspANI GGCC 2 cut(s) 676, 1107
BspCNI CTCAG 1 cut(s) 627
BspFNI CGCG 2 cut(s) 210, 1303
BspHI TCATGA 3 cut(s) 99, 109, 615
BspLI GGNNCC 2 cut(s) 1162, 1260
BspPI GGATC 2 cut(s) 749, 1205
BspT104I TTCGAA 1 cut(s) 897
BsrDI GCAATG 4 cut(s) 141, 498, 842, 1242
BsrFI RCCGGY 1 cut(s) 1048
BsrI ACTGG 2 cut(s) 484, 767
BssAI RCCGGY 1 cut(s) 1048
BssECI CCNNGG 3 cut(s) 214, 778, 874
BssMI GATC 5 cut(s) 228, 718, 741, 1135, 1210
BssSI CACGAG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 778
Bst2BI CACGAG 1 cut(s) 267
Bst2UI CCWGG 2 cut(s) 887, 1263
Bst4CI ACNGT 5 cut(s) 439, 813, 875, 998, 1403
Bst6I CTCTTC 5 cut(s) 18, 41, 98, 543, 948
BstBI TTCGAA 1 cut(s) 897
BstC8I GCNNGC 4 cut(s) 727, 928, 1050, 1320
BstDEI CTNAG 2 cut(s) 635, 792
BstDSI CCRYGG 2 cut(s) 778, 874
BstF5I GGATG 4 cut(s) 1089, 1150, 1279, 1390
BstFNI CGCG 2 cut(s) 210, 1303
BstHHI GCGC 2 cut(s) 785, 1303
BstKTI GATC 5 cut(s) 231, 721, 744, 1138, 1213
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 5 cut(s) 228, 718, 741, 1135, 1210
BstMCI CGRYCG 1 cut(s) 874
BstMWI GCNNNNNNNGC 5 cut(s) 242, 476, 590, 1049, 1319
BstNI CCWGG 2 cut(s) 887, 1263
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 214, 885, 1261
BstSFI CTRYAG 1 cut(s) 465
BstUI CGCG 2 cut(s) 210, 1303
BstV1I GCAGC 2 cut(s) 336, 1227
BstV2I GAAGAC 2 cut(s) 1230, 1404
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
BsuRI GGCC 2 cut(s) 676, 1107
BtgI CCRYGG 2 cut(s) 778, 874
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 4 cut(s) 1089, 1150, 1279, 1390
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 2 cut(s) 434, 1003
Cac8I GCNNGC 4 cut(s) 727, 928, 1050, 1320
CciI TCATGA 3 cut(s) 99, 109, 615
CfoI GCGC 2 cut(s) 785, 1303
Cfr10I RCCGGY 1 cut(s) 1048
Cfr13I GGNCC 1 cut(s) 1106
CseI GACGC 3 cut(s) 199, 518, 914
CsiI ACCWGGT 1 cut(s) 885
Csp6I GTAC 1 cut(s) 51
CviAII CATG 7 cut(s) 100, 110, 353, 616, 779, 1045, 1139
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 2 cut(s) 635, 792
DpnI GATC 5 cut(s) 230, 720, 743, 1137, 1212
DpnII GATC 5 cut(s) 228, 718, 741, 1135, 1210
DraI TTTAAA 1 cut(s) 1360
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
Eam1104I CTCTTC 5 cut(s) 18, 41, 98, 543, 948
EarI CTCTTC 5 cut(s) 18, 41, 98, 543, 948
Eco130I CCWWGG 1 cut(s) 778
Eco24I GRGCYC 2 cut(s) 641, 1368
EcoRII CCWGG 2 cut(s) 885, 1261
EcoT14I CCWWGG 1 cut(s) 778
EcoT38I GRGCYC 2 cut(s) 641, 1368
ErhI CCWWGG 1 cut(s) 778
FaeI CATG 7 cut(s) 103, 113, 356, 619, 782, 1048, 1142
FaqI GGGAC 1 cut(s) 197
FatI CATG 7 cut(s) 99, 109, 352, 615, 778, 1044, 1138
FauI CCCGC 1 cut(s) 840
FbaI TGATCA 1 cut(s) 1135
Fnu4HI GCNGC 2 cut(s) 350, 1241
FokI GGATG 4 cut(s) 1096, 1137, 1266, 1397
FriOI GRGCYC 2 cut(s) 641, 1368
Fsp4HI GCNGC 2 cut(s) 350, 1241
FspBI CTAG 5 cut(s) 57, 338, 396, 417, 732
GlaI GCGC 2 cut(s) 784, 1302
GluI GCNGC 2 cut(s) 350, 1241
HaeIII GGCC 2 cut(s) 676, 1107
HapII CCGG 3 cut(s) 216, 1049, 1164
HgaI GACGC 3 cut(s) 199, 518, 914
HhaI GCGC 2 cut(s) 785, 1303
Hin1II CATG 7 cut(s) 103, 113, 356, 619, 782, 1048, 1142
Hin6I GCGC 2 cut(s) 783, 1301
HinP1I GCGC 2 cut(s) 783, 1301
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 6 cut(s) 106, 191, 341, 505, 751, 1169
HpaII CCGG 3 cut(s) 216, 1049, 1164
HphI GGTGA 8 cut(s) 235, 427, 558, 703, 782, 875, 1046, 1178
Hpy166II GTNNAC 2 cut(s) 706, 809
Hpy188I TCNGA 5 cut(s) 636, 746, 1174, 1284, 1416
Hpy188III TCNNGA 9 cut(s) 100, 110, 338, 358, 520, 616, 758, 869, 950
Hpy8I GTNNAC 2 cut(s) 706, 809
Hpy99I CGWCG 2 cut(s) 329, 591
HpyAV CCTTC 6 cut(s) 22, 76, 235, 355, 688, 845
HpyCH4III ACNGT 5 cut(s) 439, 813, 875, 998, 1403
HpyCH4IV ACGT 1 cut(s) 1339
HpyCH4V TGCA 5 cut(s) 352, 377, 427, 491, 1289
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 476, 590, 1049, 1319
HpyF3I CTNAG 2 cut(s) 635, 792
HpySE526I ACGT 1 cut(s) 1339
Hsp92II CATG 7 cut(s) 103, 113, 356, 619, 782, 1048, 1142
HspAI GCGC 2 cut(s) 783, 1301
KroI GCCGGC 1 cut(s) 1048
KroNI GCCGGC 1 cut(s) 1050
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 5 cut(s) 228, 718, 741, 1135, 1210
Lsp1109I GCAGC 2 cut(s) 336, 1227
MabI ACCWGGT 1 cut(s) 885
MaeI CTAG 5 cut(s) 57, 338, 396, 417, 732
MaeII ACGT 1 cut(s) 1339
MaeIII GTNAC 8 cut(s) 453, 758, 902, 992, 1010, 1034, 1340, 1403
MalI GATC 5 cut(s) 230, 720, 743, 1137, 1212
MboI GATC 5 cut(s) 228, 718, 741, 1135, 1210
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 4 cut(s) 274, 595, 641, 1368
MluCI AATT 3 cut(s) 331, 921, 1291
MlyI GAGTC 2 cut(s) 115, 499
MnlI CCTC 9 cut(s) 84, 192, 302, 657, 666, 676, 731, 826, 949
MroNI GCCGGC 1 cut(s) 1048
MroXI GAANNNNTTC 1 cut(s) 855
MseI TTAA 4 cut(s) 441, 788, 1268, 1359
MslI CAYNNNNRTG 2 cut(s) 1043, 1284
MspI CCGG 3 cut(s) 216, 1049, 1164
MspR9I CCNGG 3 cut(s) 216, 887, 1263
MvaI CCWGG 2 cut(s) 887, 1263
MvnI CGCG 2 cut(s) 210, 1303
MwoI GCNNNNNNNGC 5 cut(s) 242, 476, 590, 1049, 1319
NaeI GCCGGC 1 cut(s) 1050
NciI CCSGG 1 cut(s) 216
NcoI CCATGG 1 cut(s) 778
NdeII GATC 5 cut(s) 228, 718, 741, 1135, 1210
NgoMIV GCCGGC 1 cut(s) 1048
NlaIII CATG 7 cut(s) 103, 113, 356, 619, 782, 1048, 1142
NlaIV GGNNCC 2 cut(s) 1162, 1260
NmuCI GTSAC 6 cut(s) 758, 902, 992, 1010, 1034, 1340
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 897
PagI TCATGA 3 cut(s) 99, 109, 615
PdiI GCCGGC 1 cut(s) 1050
PdmI GAANNNNTTC 1 cut(s) 855
PfeI GAWTC 4 cut(s) 191, 341, 751, 1169
PkrI GCNGC 2 cut(s) 351, 1242
PleI GAGTC 2 cut(s) 114, 499
PpsI GAGTC 2 cut(s) 114, 499
Psp6I CCWGG 2 cut(s) 885, 1261
PspGI CCWGG 2 cut(s) 885, 1261
PspN4I GGNNCC 2 cut(s) 1162, 1260
PspPI GGNCC 1 cut(s) 1106
PsuI RGATCY 1 cut(s) 228
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 2 cut(s) 1043, 1284
SaqAI TTAA 4 cut(s) 441, 788, 1268, 1359
SatI GCNGC 2 cut(s) 350, 1241
Sau3AI GATC 5 cut(s) 228, 718, 741, 1135, 1210
Sau96I GGNCC 1 cut(s) 1106
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 2 cut(s) 115, 499
ScrFI CCNGG 3 cut(s) 216, 887, 1263
SduI GDGCHC 4 cut(s) 274, 595, 641, 1368
SexAI ACCWGGT 1 cut(s) 885
SfcI CTRYAG 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 897
SmiMI CAYNNNNRTG 2 cut(s) 1043, 1284
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 3 cut(s) 331, 921, 1291
SsiI CCGC 2 cut(s) 208, 847
SspMI CTAG 5 cut(s) 57, 338, 396, 417, 732
StyD4I CCNGG 3 cut(s) 214, 885, 1261
StyI CCWWGG 1 cut(s) 778
TaaI ACNGT 5 cut(s) 439, 813, 875, 998, 1403
TaiI ACGT 1 cut(s) 1342
TaqI TCGA 8 cut(s) 44, 307, 671, 870, 897, 949, 1072, 1205
TasI AATT 3 cut(s) 331, 921, 1291
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 4 cut(s) 191, 341, 751, 1169
Tru1I TTAA 4 cut(s) 441, 788, 1268, 1359
Tru9I TTAA 4 cut(s) 441, 788, 1268, 1359
TscAI CASTG 2 cut(s) 434, 1003
TseFI GTSAC 6 cut(s) 758, 902, 992, 1010, 1034, 1340
TseI GCWGC 2 cut(s) 349, 1240
Tsp45I GTSAC 6 cut(s) 758, 902, 992, 1010, 1034, 1340
TspDTI ATGAA 7 cut(s) 116, 604, 978, 1133, 1268, 1346, 1366
TspRI CASTG 2 cut(s) 434, 1003
XapI RAATTY 1 cut(s) 1291
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 684
XmnI GAANNNNTTC 1 cut(s) 855
XspI CTAG 5 cut(s) 57, 338, 396, 417, 732
ZrmI AGTACT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.