FvH4_6g52210

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016908
Physical Location & Seq
Forward (+)
38532966 .. 38533802
837 bp
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UTR
Exon/CDS
Intron
FvH4_6g52210.t1

Sequence Viewer

Length: 426 bp
ATGTCACTCATTGCCAAATTGTTTGGAGATGAACCATTGGATCCATTCCTTTCTGTGATCAACAAGTGTCCGGTACTCAACACCCCGACTGACTGGAAGGAGACCCCTAATGCTCACGTTCTCATTTCCGACCTGCCGGGGCTGAAGAAAGAGGAGGTGAAGGTGGAGGTTGATGATGGCAGAGTACTCCAAATCAGTGGCGAGAGGAAAGTAGAAGATGGTGACGGTGGTAGTGATAAGAATGATCCTGAATATAAGTGGCACCGTGTCGAACGTAGTCGTGGCAAGTTCCTTAGGAGGTTTAAGCTACCGGAAAATGCCAAGACCGATGAGATTCAGGCTTCCATGGAGAACGGAGTGCTTACTGTCACTATCCCCAAGCAGGAGGTTCCAAAACCTCAAAGGAAGGTCGTTGAGATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

142

Amino Acids

15.96

Weight (kDa)

5.78

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 140 4.7e-28 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016908)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g52210
malus_domestica MD17G1020300.v1.1
pyrus_communis pycom17g01690
rosa_chinensis RchiOBHm_Chr2g0173761
rosa_laevigata RLG00000022219
rosa_multiflora Rmu_sc0002538.1_g000002 Rmu_sc0003188.1_g000004
rosa_roxburghii Rroxscaffold_2G00078650
rosa_rugosa Rorug02G0572600
rosa_samantha Rh2AG651500 Rh2BG662100 Rh2CG626900 Rh2DG677300
rosa_wichuraiana Rw2G053480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 261
AclWI GGATC 3 cut(s) 35, 48, 239
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 2 cut(s) 75, 186
AfiI CCNNNNNNNGG 1 cut(s) 382
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 3 cut(s) 35, 48, 239
AseI ATTAAT 1 cut(s) 420
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 169, 233
AxyI CCTNAGG 1 cut(s) 293
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 261
BccI CCATC 2 cut(s) 170, 212
BclI TGATCA 1 cut(s) 57
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 141
BmcAI AGTACT 1 cut(s) 186
Bme1390I CCNGG 1 cut(s) 138
BmiI GGNNCC 3 cut(s) 42, 263, 390
BmrFI CCNGG 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 137, 345
BsaWI WCCGGW 2 cut(s) 70, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse1I ACTGG 1 cut(s) 98
Bse21I CCTNAGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 137, 345
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMI GCAATG 1 cut(s) 9
BseNI ACTGG 1 cut(s) 98
BseRI GAGGAG 1 cut(s) 167
BshNI GGYRCC 1 cut(s) 261
BsiSI CCGG 3 cut(s) 71, 137, 311
BslI CCNNNNNNNGG 1 cut(s) 382
BsmAI GTCTC 1 cut(s) 95
Bso31I GGTCTC 1 cut(s) 95
Bsp143I GATC 3 cut(s) 40, 57, 244
Bsp19I CCATGG 1 cut(s) 345
BspLI GGNNCC 3 cut(s) 42, 263, 390
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 3 cut(s) 35, 48, 239
BspT107I GGYRCC 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 9
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 137, 345
BssMI GATC 3 cut(s) 40, 57, 244
BssT1I CCWWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 227, 266, 367
BstDEI CTNAG 1 cut(s) 293
BstDSI CCRYGG 1 cut(s) 345
BstKTI GATC 3 cut(s) 43, 60, 247
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 3 cut(s) 40, 57, 244
BstSCI CCNGG 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 1 cut(s) 40
Bsu36I CCTNAGG 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 202
BveI ACCTGC 1 cut(s) 141
Csp6I GTAC 2 cut(s) 74, 185
CviAII CATG 1 cut(s) 346
CviJI RGCY 3 cut(s) 142, 307, 341
CviKI_1 RGCY 3 cut(s) 142, 307, 341
CviQI GTAC 2 cut(s) 74, 185
DdeI CTNAG 1 cut(s) 293
DpnI GATC 3 cut(s) 42, 59, 246
DpnII GATC 3 cut(s) 40, 57, 244
Eco130I CCWWGG 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 293
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 1 cut(s) 349
FaiI YATR 2 cut(s) 255, 347
FatI CATG 1 cut(s) 345
FbaI TGATCA 1 cut(s) 57
HapII CCGG 3 cut(s) 71, 137, 311
Hin1II CATG 1 cut(s) 349
HinfI GANTC 1 cut(s) 334
HpaII CCGG 3 cut(s) 71, 137, 311
HphI GGTGA 2 cut(s) 169, 233
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 91, 154, 400
HpyCH4III ACNGT 3 cut(s) 227, 266, 367
HpyCH4IV ACGT 2 cut(s) 117, 274
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 2 cut(s) 117, 274
Hsp92II CATG 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 3 cut(s) 40, 57, 244
LpnPI CCDG 8 cut(s) 79, 84, 146, 150, 261, 323, 324, 368
MaeII ACGT 2 cut(s) 117, 274
MaeIII GTNAC 3 cut(s) 3, 221, 367
MalI GATC 3 cut(s) 42, 59, 246
MboI GATC 3 cut(s) 40, 57, 244
MboII GAAGA 2 cut(s) 157, 227
MflI RGATCY 1 cut(s) 40
MluCI AATT 2 cut(s) 17, 421
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 7 cut(s) 145, 148, 160, 198, 291, 379, 408
MseI TTAA 2 cut(s) 303, 420
MspI CCGG 3 cut(s) 71, 137, 311
MspR9I CCNGG 1 cut(s) 138
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 345
NdeII GATC 3 cut(s) 40, 57, 244
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 3 cut(s) 42, 263, 390
NmuCI GTSAC 3 cut(s) 3, 221, 367
PfeI GAWTC 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 420
PspN4I GGNNCC 3 cut(s) 42, 263, 390
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 2 cut(s) 75, 186
RsaNI GTAC 2 cut(s) 74, 185
SaqAI TTAA 2 cut(s) 303, 420
Sau3AI GATC 3 cut(s) 40, 57, 244
ScaI AGTACT 1 cut(s) 186
ScrFI CCNGG 1 cut(s) 138
Sse9I AATT 2 cut(s) 17, 421
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 3 cut(s) 227, 266, 367
TaiI ACGT 2 cut(s) 120, 277
TaqI TCGA 1 cut(s) 270
TaqII GACCGA 1 cut(s) 341
TasI AATT 2 cut(s) 17, 421
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 1 cut(s) 334
Tru1I TTAA 2 cut(s) 303, 420
Tru9I TTAA 2 cut(s) 303, 420
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 3 cut(s) 3, 221, 367
Tsp45I GTSAC 3 cut(s) 3, 221, 367
TspDTI ATGAA 1 cut(s) 45
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 1 cut(s) 202
VspI ATTAAT 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.