Rw2G053480

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016908
Physical Location & Seq
Forward (+)
81250780 .. 81251211
432 bp
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UTR
Exon/CDS
Intron
Rw2G053480.1

Sequence Viewer

Length: 432 bp
ATGTCACTCATTTCCCAATTGTTTGGGGATGAAACATTGGATCCCTTCCTTTCTATGATCAACCAGTGTCCAGTGCTCAGCACCCCAACTAATTGGAAGGAGACCCCTAAAGCCCACGTTTTCGTTTCCGACCTGCCAGGGCTGAAGAAAGCGGATGTGAAAGTGGAGGTTGACGATGGCAGAGTGCTCCAAATAAGTGGCGAGAGGAAAGTGGAAGAAGAAAATGGTGGCGGTGATAAGAATGATCGTCAGTATAAGTGGCACCGTGTCGAACGTTGCCGTGGCAAGTTCCTCAGGAGGTTTAAGCTACCGGAAAATGCCAAGACTGATGACATTCAGGCGTCCATGGAGAACGGAGTGCTTACTGTCACTGTTCCCAAGCAGGAGGTTCCAAAACCTCAAAGGAAGGTCGTTGAGATTGAAGCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

143

Amino Acids

16.27

Weight (kDa)

6.84

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 140 2.3e-26 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016908)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g52210
malus_domestica MD17G1020300.v1.1
pyrus_communis pycom17g01690
rosa_chinensis RchiOBHm_Chr2g0173761
rosa_laevigata RLG00000022219
rosa_multiflora Rmu_sc0002538.1_g000002 Rmu_sc0003188.1_g000004
rosa_roxburghii Rroxscaffold_2G00078650
rosa_rugosa Rorug02G0572600
rosa_samantha Rh2AG651500 Rh2BG662100 Rh2CG626900 Rh2DG677300
rosa_wichuraiana Rw2G053480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 261
AciI CCGC 2 cut(s) 152, 231
AclI AACGTT 1 cut(s) 274
AclWI GGATC 2 cut(s) 35, 48
AcuI CTGAAG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 341
AgsI TTSAA 1 cut(s) 422
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw21I GWGCWC 2 cut(s) 78, 189
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 2 cut(s) 35, 48
AsuHPI GGTGA 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 293
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 78, 189
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 138
BclI TGATCA 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 141
BlpI GCTNAGC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 138
BmiI GGNNCC 3 cut(s) 42, 263, 390
BmrFI CCNGG 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 137, 280, 345
BsaWI WCCGGW 1 cut(s) 310
Bse1I ACTGG 2 cut(s) 64, 71
Bse21I CCTNAGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 137, 280, 345
BseGI GGATG 2 cut(s) 34, 160
BseMII CTCAG 2 cut(s) 91, 307
BseNI ACTGG 2 cut(s) 64, 71
BshNI GGYRCC 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 78, 189
BsiSI CCGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 95
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 78, 189
Bsp143I GATC 3 cut(s) 40, 57, 244
Bsp1720I GCTNAGC 1 cut(s) 77
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 2 cut(s) 152, 231
BspCNI CTCAG 2 cut(s) 90, 306
BspLI GGNNCC 3 cut(s) 42, 263, 390
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 35, 48
BspT107I GGYRCC 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 95
BsrI ACTGG 2 cut(s) 64, 71
BssECI CCNNGG 3 cut(s) 137, 280, 345
BssMI GATC 3 cut(s) 40, 57, 244
BssNI GRCGYC 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 3 cut(s) 266, 367, 373
BstACI GRCGYC 1 cut(s) 341
BstDEI CTNAG 2 cut(s) 77, 293
BstDSI CCRYGG 2 cut(s) 280, 345
BstF5I GGATG 2 cut(s) 34, 160
BstKTI GATC 3 cut(s) 43, 60, 247
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 3 cut(s) 40, 57, 244
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 3 cut(s) 23, 93, 197
BstYI RGATCY 1 cut(s) 40
Bsu36I CCTNAGG 1 cut(s) 293
BtgI CCRYGG 2 cut(s) 280, 345
BtsCI GGATG 2 cut(s) 34, 160
BtsIMutI CAGTG 3 cut(s) 71, 78, 369
BveI ACCTGC 1 cut(s) 141
CseI GACGC 1 cut(s) 330
CviAII CATG 1 cut(s) 346
CviJI RGCY 3 cut(s) 113, 142, 307
CviKI_1 RGCY 3 cut(s) 113, 142, 307
DdeI CTNAG 2 cut(s) 77, 293
DpnI GATC 3 cut(s) 42, 59, 246
DpnII GATC 3 cut(s) 40, 57, 244
Eco130I CCWWGG 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 1 cut(s) 349
FaiI YATR 3 cut(s) 56, 255, 347
FatI CATG 1 cut(s) 345
FbaI TGATCA 1 cut(s) 57
FokI GGATG 2 cut(s) 41, 167
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HpaII CCGG 1 cut(s) 311
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 1 cut(s) 172
HpyAV CCTTC 3 cut(s) 55, 91, 400
HpyCH4III ACNGT 3 cut(s) 266, 367, 373
HpyCH4IV ACGT 2 cut(s) 117, 274
HpyF3I CTNAG 2 cut(s) 77, 293
HpySE526I ACGT 2 cut(s) 117, 274
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 3 cut(s) 40, 57, 244
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 9 cut(s) 77, 84, 123, 146, 150, 280, 323, 324, 368
MaeII ACGT 2 cut(s) 117, 274
MaeIII GTNAC 2 cut(s) 3, 367
MalI GATC 3 cut(s) 42, 59, 246
MboI GATC 3 cut(s) 40, 57, 244
MboII GAAGA 3 cut(s) 157, 227, 230
MfeI CAATTG 1 cut(s) 17
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 78, 189
MluCI AATT 2 cut(s) 17, 91
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 6 cut(s) 160, 198, 291, 302, 379, 408
MseI TTAA 2 cut(s) 303, 430
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 138
MunI CAATTG 1 cut(s) 17
MvaI CCWGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 345
NdeII GATC 3 cut(s) 40, 57, 244
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 3 cut(s) 42, 263, 390
NmuCI GTSAC 2 cut(s) 3, 367
Psp1406I AACGTT 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 42, 263, 390
PsuI RGATCY 1 cut(s) 40
SaqAI TTAA 2 cut(s) 303, 430
Sau3AI GATC 3 cut(s) 40, 57, 244
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 2 cut(s) 78, 189
SetI ASST 9 cut(s) 120, 135, 171, 277, 302, 309, 390, 400, 411
Sse9I AATT 2 cut(s) 17, 91
SsiI CCGC 2 cut(s) 152, 231
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 3 cut(s) 266, 367, 373
TaiI ACGT 2 cut(s) 120, 277
TaqI TCGA 1 cut(s) 270
TasI AATT 2 cut(s) 17, 91
Tru1I TTAA 2 cut(s) 303, 430
Tru9I TTAA 2 cut(s) 303, 430
TscAI CASTG 3 cut(s) 71, 78, 376
TseFI GTSAC 2 cut(s) 3, 367
Tsp45I GTSAC 2 cut(s) 3, 367
TspDTI ATGAA 1 cut(s) 45
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 3 cut(s) 71, 78, 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.