FvH4_7g02331

Methylenetetrahydrofolate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0021106
Physical Location & Seq
Forward (+)
2644443 .. 2645397
955 bp
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UTR
Exon/CDS
Intron
FvH4_7g02331.t1

Sequence Viewer

Length: 570 bp
ATGGACGTTGTTTCAATGATCCGGAGAAGCTTTGACGATATTCATGACGAGTTTGAGAAACTCTGCTGTTGTTTGCCTCTAAAATGTATTTTTTGCCCAAGCCCTAATTGGCCAGAACAAGACAGGCTTGATCAGCAGCTGCCAGCAGAGACGAGTAAGATCAAGGAGGAACTCAACCAAAAGGGTTTCCTTGTCATCGACAGCCAAGCTGCTGTAAATGGGGAGAGATTTGATTCGGCCTCTTTCGGAGGATTTGTGTATCAAAAAGCCTATGTGGAGTTTTTCTGCTCCAAGGAGACACTGGATGTTCTTATCGAAAAATGCAAGGCTCATCCACCTCTGAGCTACATGGCTTTGAACAAACAAGGGAGTTGTTGGATATCTAATCTTGATCGGAGTAATATCAATGCAGTGACATGGGGAGTCTTCCCTACGAAAGAGATAATCGAACAGACTGTCGATGCGGCTAGGTTTAGGGTTTGGAAGGATGGGGCTTTTGAAAGTTGGTCAGAGGAATTACTTGAACACCAGGGCGGGGAACAACTACTTCTTGGTGAGCCTGGTAGATAA

Protein Analysis

190

Amino Acids

21.56

Weight (kDa)

4.82

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTHFR_C PF21895 44 - 172 1.7e-25 MTHFR, SAM-binding regulatory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021106)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02331
rosa_chinensis RchiOBHm_Chr1g0319671
rosa_laevigata RLG00000030465
rosa_roxburghii Rroxscaffold_4G00328220
rosa_samantha Rh1BG038300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 2 cut(s) 464, 534
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 535
AgsI TTSAA 4 cut(s) 15, 358, 500, 524
AjnI CCWGG 2 cut(s) 528, 559
AluBI AGCT 4 cut(s) 30, 139, 209, 345
AluI AGCT 4 cut(s) 30, 139, 209, 345
Alw26I GTCTC 2 cut(s) 143, 290
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 139
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 2 cut(s) 110, 237
ApeKI GCWGC 3 cut(s) 136, 139, 209
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
AsuHPI GGTGA 1 cut(s) 566
BalI TGGCCA 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 418
BbvI GCAGC 3 cut(s) 126, 148, 196
BccI CCATC 1 cut(s) 482
BciT130I CCWGG 2 cut(s) 530, 561
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 143, 290
BfaI CTAG 1 cut(s) 468
BisI GCNGC 4 cut(s) 137, 140, 210, 465
BlsI GCNGC 4 cut(s) 138, 141, 211, 466
Bme1390I CCNGG 2 cut(s) 530, 561
BmrFI CCNGG 2 cut(s) 530, 561
BmsI GCATC 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 291, 529
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse1I ACTGG 1 cut(s) 306
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 2 cut(s) 530, 561
BseDI CCNNGG 2 cut(s) 291, 529
BseGI GGATG 3 cut(s) 310, 331, 493
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 1 cut(s) 306
BseXI GCAGC 3 cut(s) 126, 148, 196
BshFI GGCC 2 cut(s) 112, 239
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 535
BsmAI GTCTC 2 cut(s) 143, 290
BsmBI CGTCTC 1 cut(s) 143
BsnI GGCC 2 cut(s) 112, 239
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 4 cut(s) 18, 130, 159, 391
BspACI CCGC 2 cut(s) 464, 534
BspANI GGCC 2 cut(s) 112, 239
BspCNI CTCAG 1 cut(s) 333
BspEI TCCGGA 1 cut(s) 21
BspHI TCATGA 1 cut(s) 43
BspPI GGATC 1 cut(s) 13
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 2 cut(s) 291, 529
BssMI GATC 4 cut(s) 18, 130, 159, 391
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 530, 561
Bst4CI ACNGT 1 cut(s) 457
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 1 cut(s) 341
BstF5I GGATG 3 cut(s) 310, 331, 493
BstKTI GATC 4 cut(s) 21, 133, 162, 394
BstMAI GTCTC 2 cut(s) 143, 290
BstMBI GATC 4 cut(s) 18, 130, 159, 391
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 2 cut(s) 530, 561
BstSCI CCNGG 2 cut(s) 528, 559
BstV1I GCAGC 3 cut(s) 126, 148, 196
BstV2I GAAGAC 1 cut(s) 418
BsuRI GGCC 2 cut(s) 112, 239
BtsCI GGATG 3 cut(s) 310, 331, 493
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 299, 417
Cac8I GCNNGC 1 cut(s) 144
CaiI CAGNNNCTG 1 cut(s) 139
CciI TCATGA 1 cut(s) 43
CviAII CATG 3 cut(s) 44, 349, 417
DdeI CTNAG 1 cut(s) 341
DpnI GATC 4 cut(s) 20, 132, 161, 393
DpnII GATC 4 cut(s) 18, 130, 159, 391
EaeI YGGCCR 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 291
Eco32I GATATC 1 cut(s) 381
EcoRII CCWGG 2 cut(s) 528, 559
EcoRV GATATC 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 3 cut(s) 47, 352, 420
FaiI YATR 4 cut(s) 45, 273, 350, 418
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FatI CATG 3 cut(s) 43, 348, 416
FauI CCCGC 1 cut(s) 527
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 4 cut(s) 137, 140, 210, 465
FokI GGATG 3 cut(s) 317, 318, 500
Fsp4HI GCNGC 4 cut(s) 137, 140, 210, 465
FspBI CTAG 1 cut(s) 468
GluI GCNGC 4 cut(s) 137, 140, 210, 465
HaeIII GGCC 2 cut(s) 112, 239
HapII CCGG 1 cut(s) 22
Hin1II CATG 3 cut(s) 47, 352, 420
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 2 cut(s) 233, 423
HpaII CCGG 1 cut(s) 22
HphI GGTGA 1 cut(s) 566
Hpy188I TCNGA 4 cut(s) 248, 342, 396, 511
Hpy188III TCNNGA 3 cut(s) 22, 44, 389
HpyAV CCTTC 1 cut(s) 478
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 2 cut(s) 324, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 3 cut(s) 47, 352, 420
Kpn2I TCCGGA 1 cut(s) 21
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 4 cut(s) 18, 130, 159, 391
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 8 cut(s) 35, 109, 126, 156, 287, 515, 542, 546
Lsp1109I GCAGC 3 cut(s) 126, 148, 196
LweI GCATC 1 cut(s) 451
MaeI CTAG 1 cut(s) 468
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 412
MalI GATC 4 cut(s) 20, 132, 161, 393
MboI GATC 4 cut(s) 18, 130, 159, 391
MboII GAAGA 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 112
MluCI AATT 2 cut(s) 106, 515
MluNI TGGCCA 1 cut(s) 112
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 6 cut(s) 87, 160, 242, 250, 348, 505
Mox20I TGGCCA 1 cut(s) 112
MroI TCCGGA 1 cut(s) 21
MscI TGGCCA 1 cut(s) 112
Msp20I TGGCCA 1 cut(s) 112
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 2 cut(s) 530, 561
MvaI CCWGG 2 cut(s) 530, 561
MwoI GCNNNNNNNGC 1 cut(s) 133
NdeII GATC 4 cut(s) 18, 130, 159, 391
NlaIII CATG 3 cut(s) 47, 352, 420
NmuCI GTSAC 1 cut(s) 412
PagI TCATGA 1 cut(s) 43
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 4 cut(s) 138, 141, 211, 466
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
Psp6I CCWGG 2 cut(s) 528, 559
PspGI CCWGG 2 cut(s) 528, 559
PstNI CAGNNNCTG 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 139
SatI GCNGC 4 cut(s) 137, 140, 210, 465
Sau3AI GATC 4 cut(s) 18, 130, 159, 391
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 2 cut(s) 530, 561
SetI ASST 7 cut(s) 9, 32, 141, 211, 340, 347, 473
SfaNI GCATC 1 cut(s) 451
Sse9I AATT 2 cut(s) 106, 515
SsiI CCGC 2 cut(s) 464, 534
SspMI CTAG 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 528, 559
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 1 cut(s) 9
TaqI TCGA 4 cut(s) 198, 315, 447, 459
TasI AATT 2 cut(s) 106, 515
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 1 cut(s) 233
TscAI CASTG 2 cut(s) 306, 417
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 3 cut(s) 136, 139, 209
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 1 cut(s) 32
TspRI CASTG 2 cut(s) 306, 417
XcmI CCANNNNNNNNNTGG 2 cut(s) 105, 298
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.