Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0021106

Physical Location & Seq

Genomic Coordinates Forward (+)

5091352 .. 5093252

Gene Structure 1901 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG038300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 525 bp

ATGCAATATATCAGAATACAATTTGAACAAGCCGGGCTTGATCAGTTGCCAGAGACGAAGAAGATCAAGAATGAACTGCTGGAGGAACTTAACAGAAAGGGTTTCATTATCATTAACAGCCAAGCAGCAGTAAATGGGGAAAGATTCGATTCGGCATCTTTCAGAGGATATGTGTATCAAAAAGCCTATGTGGAGTTTTTCTGTTCCAAGGAGACATTGGATGTTCTTATCGAAAAATGCAAGGCTCATCCATCTCTAACCTACATGGCCGTGAACAGACAAGGTAGTTGTTGGATATCTAATCATGATCAGAGTAATATCAATGCAGTTACATGGGGAGTCTTCCCTGGGAAGGAGATAATCGAACGGACTGTCGACGCGACTAGGTTTAGGATTTGGAAGGATGAGGCTTTCGAAATTTGGTCAGAGAAATTACTTGAACAGGGGAACAACTACTTCTTGGTTAGCTTGGTAGATAATGACTACATCCATGGTGACCTTTCCATCATTTTTCCTGATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004489 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 ko01523 map00670 map00720 map01100 map01120 map01200 map01523

Pfam Domains

Protein Families

MTHFR

Protein Analysis

174

Amino Acids

20.26

Weight (kDa)

5.13

Isoelectric Point (pI)

31.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTHFR_C	PF21895	17 - 163	1.1e-30	MTHFR, SAM-binding regulatory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021106)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g02331
rosa_chinensis	RchiOBHm_Chr1g0319671
rosa_laevigata	RLG00000030465
rosa_roxburghii	Rroxscaffold_4G00328220
rosa_samantha	Rh1BG038300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	375
AccII	CGCG	1 cut(s)	380
AcoI	YGGCCR	1 cut(s)	267
AcsI	RAATTY	1 cut(s)	417
AfiI	CCNNNNNNNGG	1 cut(s)	352
AgsI	TTSAA	2 cut(s)	26, 440
AjnI	CCWGG	1 cut(s)	346
AluBI	AGCT	1 cut(s)	468
AluI	AGCT	1 cut(s)	468
Alw26I	GTCTC	2 cut(s)	47, 206
AoxI	GGCC	1 cut(s)	267
ApeKI	GCWGC	1 cut(s)	125
ApoI	RAATTY	1 cut(s)	417
ArsI	GACNNNNNNTTYG	2 cut(s)	357, 389
AsuC2I	CCSGG	1 cut(s)	34
AsuHPI	GGTGA	1 cut(s)	506
AsuII	TTCGAA	1 cut(s)	414
BbsI	GAAGAC	1 cut(s)	334
BbvI	GCAGC	1 cut(s)	137
BccI	CCATC	2 cut(s)	259, 512
BceAI	ACGGC	1 cut(s)	254
BciT130I	CCWGG	1 cut(s)	348
BclI	TGATCA	2 cut(s)	40, 307
BcnI	CCSGG	1 cut(s)	34
BcoDI	GTCTC	2 cut(s)	47, 206
BfaI	CTAG	1 cut(s)	384
BisI	GCNGC	1 cut(s)	126
BlsI	GCNGC	1 cut(s)	127
Bme1390I	CCNGG	2 cut(s)	34, 348
BmrFI	CCNGG	2 cut(s)	34, 348
BmsI	GCATC	1 cut(s)	164
BpiI	GAAGAC	1 cut(s)	334
BpmI	CTGGAG	1 cut(s)	101
Bpu14I	TTCGAA	1 cut(s)	414
BpuMI	CCSGG	1 cut(s)	34
BsaJI	CCNNGG	4 cut(s)	207, 346, 347, 490
Bsc4I	CCNNNNNNNGG	1 cut(s)	352
BseBI	CCWGG	1 cut(s)	348
BseDI	CCNNGG	4 cut(s)	207, 346, 347, 490
BseGI	GGATG	4 cut(s)	226, 247, 409, 486
BseLI	CCNNNNNNNGG	1 cut(s)	352
BseXI	GCAGC	1 cut(s)	137
Bsh1236I	CGCG	1 cut(s)	380
BshFI	GGCC	1 cut(s)	269
BsiSI	CCGG	1 cut(s)	33
BslI	CCNNNNNNNGG	1 cut(s)	352
BsmAI	GTCTC	2 cut(s)	47, 206
BsmBI	CGTCTC	1 cut(s)	47
BsnI	GGCC	1 cut(s)	269
Bsp119I	TTCGAA	1 cut(s)	414
Bsp143I	GATC	3 cut(s)	40, 63, 307
Bsp19I	CCATGG	1 cut(s)	490
BspANI	GGCC	1 cut(s)	269
BspFNI	CGCG	1 cut(s)	380
BspHI	TCATGA	1 cut(s)	304
BspT104I	TTCGAA	1 cut(s)	414
BssECI	CCNNGG	4 cut(s)	207, 346, 347, 490
BssMI	GATC	3 cut(s)	40, 63, 307
BssT1I	CCWWGG	2 cut(s)	207, 490
Bst2UI	CCWGG	1 cut(s)	348
Bst4CI	ACNGT	1 cut(s)	373
BstBI	TTCGAA	1 cut(s)	414
BstDSI	CCRYGG	1 cut(s)	490
BstEII	GGTNACC	1 cut(s)	494
BstF5I	GGATG	4 cut(s)	226, 247, 409, 486
BstFNI	CGCG	1 cut(s)	380
BstKTI	GATC	3 cut(s)	43, 66, 310
BstMAI	GTCTC	2 cut(s)	47, 206
BstMBI	GATC	3 cut(s)	40, 63, 307
BstNI	CCWGG	1 cut(s)	348
BstPI	GGTNACC	1 cut(s)	494
BstSCI	CCNGG	2 cut(s)	32, 346
BstUI	CGCG	1 cut(s)	380
BstV1I	GCAGC	1 cut(s)	137
BstV2I	GAAGAC	1 cut(s)	334
BsuRI	GGCC	1 cut(s)	269
BtgI	CCRYGG	1 cut(s)	490
BtsCI	GGATG	4 cut(s)	226, 247, 409, 486
CciI	TCATGA	1 cut(s)	304
CseI	GACGC	1 cut(s)	386
CviAII	CATG	4 cut(s)	265, 305, 333, 491
CviJI	RGCY	8 cut(s)	32, 37, 120, 185, 245, 269, 410, 468
CviKI_1	RGCY	8 cut(s)	32, 37, 120, 185, 245, 269, 410, 468
DpnI	GATC	3 cut(s)	42, 65, 309
DpnII	GATC	3 cut(s)	40, 63, 307
EaeI	YGGCCR	1 cut(s)	267
Eco130I	CCWWGG	2 cut(s)	207, 490
Eco32I	GATATC	1 cut(s)	297
Eco91I	GGTNACC	1 cut(s)	494
EcoO65I	GGTNACC	1 cut(s)	494
EcoRII	CCWGG	1 cut(s)	346
EcoRV	GATATC	1 cut(s)	297
EcoT14I	CCWWGG	2 cut(s)	207, 490
ErhI	CCWWGG	2 cut(s)	207, 490
Esp3I	CGTCTC	1 cut(s)	47
FaeI	CATG	4 cut(s)	268, 308, 336, 494
FaiI	YATR	7 cut(s)	9, 171, 189, 266, 306, 334, 492
FalI	AAGNNNNNCTT	2 cut(s)	21, 53
FatI	CATG	4 cut(s)	264, 304, 332, 490
FbaI	TGATCA	2 cut(s)	40, 307
FblI	GTMKAC	1 cut(s)	375
Fnu4HI	GCNGC	1 cut(s)	126
FokI	GGATG	4 cut(s)	233, 234, 416, 473
Fsp4HI	GCNGC	1 cut(s)	126
FspBI	CTAG	1 cut(s)	384
GluI	GCNGC	1 cut(s)	126
GsuI	CTGGAG	1 cut(s)	101
HaeIII	GGCC	1 cut(s)	269
HapII	CCGG	1 cut(s)	33
HgaI	GACGC	1 cut(s)	386
Hin1II	CATG	4 cut(s)	268, 308, 336, 494
HincII	GTYRAC	1 cut(s)	376
HindII	GTYRAC	1 cut(s)	376
HinfI	GANTC	3 cut(s)	144, 149, 339
HpaII	CCGG	1 cut(s)	33
HphI	GGTGA	1 cut(s)	506
Hpy166II	GTNNAC	2 cut(s)	274, 376
Hpy188I	TCNGA	4 cut(s)	14, 164, 312, 427
Hpy188III	TCNNGA	3 cut(s)	67, 305, 515
Hpy8I	GTNNAC	2 cut(s)	274, 376
Hpy99I	CGWCG	1 cut(s)	380
HpyAV	CCTTC	2 cut(s)	346, 394
HpyCH4III	ACNGT	1 cut(s)	373
HpyCH4V	TGCA	3 cut(s)	4, 240, 326
Hsp92II	CATG	4 cut(s)	268, 308, 336, 494
Ksp22I	TGATCA	2 cut(s)	40, 307
Kzo9I	GATC	3 cut(s)	40, 63, 307
LpnPI	CCDG	6 cut(s)	46, 63, 65, 333, 360, 428
Lsp1109I	GCAGC	1 cut(s)	137
LweI	GCATC	1 cut(s)	164
MaeI	CTAG	1 cut(s)	384
MaeIII	GTNAC	2 cut(s)	328, 494
MalI	GATC	3 cut(s)	42, 65, 309
MboI	GATC	3 cut(s)	40, 63, 307
MboII	GAAGA	3 cut(s)	70, 73, 334
MluCI	AATT	3 cut(s)	20, 417, 431
MlyI	GAGTC	1 cut(s)	348
MmeI	TCCRAC	1 cut(s)	272
MnlI	CCTC	3 cut(s)	76, 158, 400
MseI	TTAA	2 cut(s)	90, 114
MslI	CAYNNNNRTG	1 cut(s)	269
MspI	CCGG	1 cut(s)	33
MspR9I	CCNGG	2 cut(s)	34, 348
MvaI	CCWGG	1 cut(s)	348
MvnI	CGCG	1 cut(s)	380
NciI	CCSGG	1 cut(s)	34
NcoI	CCATGG	1 cut(s)	490
NdeII	GATC	3 cut(s)	40, 63, 307
NlaIII	CATG	4 cut(s)	268, 308, 336, 494
NmuCI	GTSAC	1 cut(s)	494
NspV	TTCGAA	1 cut(s)	414
PagI	TCATGA	1 cut(s)	304
PasI	CCCWGGG	1 cut(s)	347
PfeI	GAWTC	2 cut(s)	144, 149
PkrI	GCNGC	1 cut(s)	127
PleI	GAGTC	1 cut(s)	347
PpsI	GAGTC	1 cut(s)	347
Psp6I	CCWGG	1 cut(s)	346
PspEI	GGTNACC	1 cut(s)	494
PspGI	CCWGG	1 cut(s)	346
RseI	CAYNNNNRTG	1 cut(s)	269
SalI	GTCGAC	1 cut(s)	374
SaqAI	TTAA	2 cut(s)	90, 114
SatI	GCNGC	1 cut(s)	126
Sau3AI	GATC	3 cut(s)	40, 63, 307
SchI	GAGTC	1 cut(s)	348
ScrFI	CCNGG	2 cut(s)	34, 348
SetI	ASST	5 cut(s)	263, 286, 389, 470, 501
SfaNI	GCATC	1 cut(s)	164
SfuI	TTCGAA	1 cut(s)	414
SmiMI	CAYNNNNRTG	1 cut(s)	269
Sse9I	AATT	3 cut(s)	20, 417, 431
SspMI	CTAG	1 cut(s)	384
StyD4I	CCNGG	2 cut(s)	32, 346
StyI	CCWWGG	2 cut(s)	207, 490
TaaI	ACNGT	1 cut(s)	373
TaqI	TCGA	5 cut(s)	147, 231, 363, 375, 414
TasI	AATT	3 cut(s)	20, 417, 431
TfiI	GAWTC	2 cut(s)	144, 149
Tru1I	TTAA	2 cut(s)	90, 114
Tru9I	TTAA	2 cut(s)	90, 114
TseFI	GTSAC	1 cut(s)	494
TseI	GCWGC	1 cut(s)	125
Tsp45I	GTSAC	1 cut(s)	494
TspDTI	ATGAA	2 cut(s)	87, 94
TspGWI	ACGGA	1 cut(s)	382
XapI	RAATTY	1 cut(s)	417
XcmI	CCANNNNNNNNNTGG	1 cut(s)	214
XmiI	GTMKAC	1 cut(s)	375
XspI	CTAG	1 cut(s)	384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.