Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0021106

Physical Location & Seq

Genomic Coordinates Reverse (-)

61134412 .. 61138127

Gene Structure 3716 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00328220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 480 bp

ATGGAGACTGGAGGATGGCGTCAATGGAAGTTTAAGAGCTCTTGTTCCACAACGATTCAGAGAAAAAAGAAAAGGAAGAGAACAGTAAATGGGGAAAGATTCGATTCGGCATCTTTCGGAGGATATGTGCATCAAAAAGCCTATGTGGAGTTTTTCTGTTCCAAGGAGACAGTGGATATTCTTATCGAAAAATGCAAGGCTCATCCATCTCTGACCTATATGGCCGTGAACAGACAAGGTAGTTGTTGGATATCTAATCTTAATCGGAGTAATATCAATGCAGTTACATGGGGAGTCTTCCCTAGGAAGGAGATAATCGAACGGACTGTCGACGCGGCTAGGTTTAGGGTTTGGAAGGATGAGGTTTTCGAAATTTGGTCGGAGAAATTACTTGAACAGGGGAACAACTACTTCTTAGTTAGCTTGGTAGATAATAACTACATCCATGGTGACCTTTCCACCATTTTTCCTGATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004489 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 ko01523 map00670 map00720 map01100 map01120 map01200 map01523

Pfam Domains

Protein Families

MTHFR

Protein Analysis

159

Amino Acids

18.62

Weight (kDa)

9.21

Isoelectric Point (pI)

40.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTHFR_C	PF21895	39 - 147	5.9e-22	MTHFR, SAM-binding regulatory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0021106)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g02331
rosa_chinensis	RchiOBHm_Chr1g0319671
rosa_laevigata	RLG00000030465
rosa_roxburghii	Rroxscaffold_4G00328220
rosa_samantha	Rh1BG038300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	330
AccII	CGCG	1 cut(s)	335
AciI	CCGC	1 cut(s)	335
AcoI	YGGCCR	1 cut(s)	222
AcsI	RAATTY	1 cut(s)	372
AcyI	GRCGYC	1 cut(s)	19
AfiI	CCNNNNNNNGG	1 cut(s)	307
AgsI	TTSAA	1 cut(s)	395
AluBI	AGCT	2 cut(s)	39, 423
AluI	AGCT	2 cut(s)	39, 423
Alw21I	GWGCWC	1 cut(s)	41
Alw26I	GTCTC	1 cut(s)	161
AoxI	GGCC	1 cut(s)	222
ApoI	RAATTY	1 cut(s)	372
ArsI	GACNNNNNNTTYG	2 cut(s)	312, 344
AspA2I	CCTAGG	1 cut(s)	302
AsuHPI	GGTGA	1 cut(s)	461
AsuII	TTCGAA	1 cut(s)	369
AvrII	CCTAGG	1 cut(s)	302
BanII	GRGCYC	1 cut(s)	41
BbsI	GAAGAC	1 cut(s)	289
Bbv12I	GWGCWC	1 cut(s)	41
BccI	CCATC	2 cut(s)	9, 214
BceAI	ACGGC	1 cut(s)	209
BcoDI	GTCTC	1 cut(s)	161
BfaI	CTAG	2 cut(s)	303, 339
BisI	GCNGC	1 cut(s)	336
BlnI	CCTAGG	1 cut(s)	302
BlsI	GCNGC	1 cut(s)	337
BmsI	GCATC	2 cut(s)	119, 139
BpiI	GAAGAC	1 cut(s)	289
BpmI	CTGGAG	1 cut(s)	30
Bpu14I	TTCGAA	1 cut(s)	369
BsaHI	GRCGYC	1 cut(s)	19
BsaJI	CCNNGG	3 cut(s)	162, 302, 445
BsaXI	ACNNNNNCTCC	2 cut(s)	111, 141
Bsc4I	CCNNNNNNNGG	1 cut(s)	307
Bse1I	ACTGG	1 cut(s)	13
BseDI	CCNNGG	3 cut(s)	162, 302, 445
BseGI	GGATG	4 cut(s)	20, 202, 364, 441
BseLI	CCNNNNNNNGG	1 cut(s)	307
BseNI	ACTGG	1 cut(s)	13
Bsh1236I	CGCG	1 cut(s)	335
BshFI	GGCC	1 cut(s)	224
BsiHKAI	GWGCWC	1 cut(s)	41
BslI	CCNNNNNNNGG	1 cut(s)	307
BsmAI	GTCTC	1 cut(s)	161
BsnI	GGCC	1 cut(s)	224
Bsp119I	TTCGAA	1 cut(s)	369
Bsp1286I	GDGCHC	1 cut(s)	41
Bsp19I	CCATGG	1 cut(s)	445
BspACI	CCGC	1 cut(s)	335
BspANI	GGCC	1 cut(s)	224
BspFNI	CGCG	1 cut(s)	335
BspT104I	TTCGAA	1 cut(s)	369
BsrI	ACTGG	1 cut(s)	13
BssECI	CCNNGG	3 cut(s)	162, 302, 445
BssNI	GRCGYC	1 cut(s)	19
BssT1I	CCWWGG	3 cut(s)	162, 302, 445
Bst4CI	ACNGT	3 cut(s)	85, 172, 328
Bst6I	CTCTTC	1 cut(s)	71
BstACI	GRCGYC	1 cut(s)	19
BstBI	TTCGAA	1 cut(s)	369
BstDEI	CTNAG	1 cut(s)	415
BstDSI	CCRYGG	1 cut(s)	445
BstEII	GGTNACC	1 cut(s)	449
BstF5I	GGATG	4 cut(s)	20, 202, 364, 441
BstFNI	CGCG	1 cut(s)	335
BstMAI	GTCTC	1 cut(s)	161
BstPI	GGTNACC	1 cut(s)	449
BstUI	CGCG	1 cut(s)	335
BstV2I	GAAGAC	1 cut(s)	289
BsuRI	GGCC	1 cut(s)	224
BtgI	CCRYGG	1 cut(s)	445
BtsCI	GGATG	4 cut(s)	20, 202, 364, 441
BtsIMutI	CAGTG	1 cut(s)	177
CseI	GACGC	2 cut(s)	8, 341
CviAII	CATG	2 cut(s)	288, 446
CviJI	RGCY	6 cut(s)	39, 140, 200, 224, 338, 423
CviKI_1	RGCY	6 cut(s)	39, 140, 200, 224, 338, 423
DdeI	CTNAG	1 cut(s)	415
EaeI	YGGCCR	1 cut(s)	222
Eam1104I	CTCTTC	1 cut(s)	71
EarI	CTCTTC	1 cut(s)	71
Ecl136II	GAGCTC	1 cut(s)	39
Eco130I	CCWWGG	3 cut(s)	162, 302, 445
Eco24I	GRGCYC	1 cut(s)	41
Eco32I	GATATC	1 cut(s)	252
Eco53kI	GAGCTC	1 cut(s)	39
Eco91I	GGTNACC	1 cut(s)	449
EcoICRI	GAGCTC	1 cut(s)	39
EcoO65I	GGTNACC	1 cut(s)	449
EcoRV	GATATC	1 cut(s)	252
EcoT14I	CCWWGG	3 cut(s)	162, 302, 445
EcoT38I	GRGCYC	1 cut(s)	41
ErhI	CCWWGG	3 cut(s)	162, 302, 445
FaeI	CATG	2 cut(s)	291, 449
FaiI	YATR	6 cut(s)	126, 144, 219, 221, 289, 447
FatI	CATG	2 cut(s)	287, 445
FblI	GTMKAC	1 cut(s)	330
Fnu4HI	GCNGC	1 cut(s)	336
FokI	GGATG	4 cut(s)	27, 189, 371, 428
FriOI	GRGCYC	1 cut(s)	41
Fsp4HI	GCNGC	1 cut(s)	336
FspBI	CTAG	2 cut(s)	303, 339
GluI	GCNGC	1 cut(s)	336
GsuI	CTGGAG	1 cut(s)	30
HaeIII	GGCC	1 cut(s)	224
HgaI	GACGC	2 cut(s)	8, 341
Hin1I	GRCGYC	1 cut(s)	19
Hin1II	CATG	2 cut(s)	291, 449
HincII	GTYRAC	1 cut(s)	331
HindII	GTYRAC	1 cut(s)	331
HinfI	GANTC	4 cut(s)	55, 99, 104, 294
HphI	GGTGA	1 cut(s)	461
Hpy166II	GTNNAC	2 cut(s)	229, 331
Hpy188I	TCNGA	5 cut(s)	60, 119, 213, 267, 382
Hpy188III	TCNNGA	1 cut(s)	470
Hpy8I	GTNNAC	2 cut(s)	229, 331
Hpy99I	CGWCG	1 cut(s)	335
HpyAV	CCTTC	2 cut(s)	301, 349
HpyCH4III	ACNGT	3 cut(s)	85, 172, 328
HpyCH4V	TGCA	3 cut(s)	130, 195, 281
HpyF3I	CTNAG	1 cut(s)	415
Hsp92I	GRCGYC	1 cut(s)	19
Hsp92II	CATG	2 cut(s)	291, 449
LpnPI	CCDG	1 cut(s)	383
LweI	GCATC	2 cut(s)	119, 139
MaeI	CTAG	2 cut(s)	303, 339
MaeIII	GTNAC	2 cut(s)	283, 449
MboII	GAAGA	2 cut(s)	88, 289
MhlI	GDGCHC	1 cut(s)	41
MluCI	AATT	2 cut(s)	372, 386
MlyI	GAGTC	1 cut(s)	303
MmeI	TCCRAC	2 cut(s)	227, 360
MnlI	CCTC	3 cut(s)	5, 113, 355
MseI	TTAA	2 cut(s)	33, 261
MvnI	CGCG	1 cut(s)	335
NcoI	CCATGG	1 cut(s)	445
NlaIII	CATG	2 cut(s)	291, 449
NmuCI	GTSAC	1 cut(s)	449
NspV	TTCGAA	1 cut(s)	369
PfeI	GAWTC	3 cut(s)	55, 99, 104
PkrI	GCNGC	1 cut(s)	337
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
Psp124BI	GAGCTC	1 cut(s)	41
PspEI	GGTNACC	1 cut(s)	449
SacI	GAGCTC	1 cut(s)	41
SalI	GTCGAC	1 cut(s)	329
SaqAI	TTAA	2 cut(s)	33, 261
SatI	GCNGC	1 cut(s)	336
SchI	GAGTC	1 cut(s)	303
SduI	GDGCHC	1 cut(s)	41
SetI	ASST	7 cut(s)	41, 218, 241, 344, 366, 425, 456
SfaNI	GCATC	2 cut(s)	119, 139
SfuI	TTCGAA	1 cut(s)	369
Sse9I	AATT	2 cut(s)	372, 386
SsiI	CCGC	1 cut(s)	335
SspMI	CTAG	2 cut(s)	303, 339
SstI	GAGCTC	1 cut(s)	41
StyI	CCWWGG	3 cut(s)	162, 302, 445
TaaI	ACNGT	3 cut(s)	85, 172, 328
TaqI	TCGA	5 cut(s)	102, 186, 318, 330, 369
TasI	AATT	2 cut(s)	372, 386
TauI	GCSGC	1 cut(s)	338
TfiI	GAWTC	3 cut(s)	55, 99, 104
Tru1I	TTAA	2 cut(s)	33, 261
Tru9I	TTAA	2 cut(s)	33, 261
TscAI	CASTG	1 cut(s)	177
TseFI	GTSAC	1 cut(s)	449
Tsp45I	GTSAC	1 cut(s)	449
TspGWI	ACGGA	1 cut(s)	337
TspRI	CASTG	1 cut(s)	177
XapI	RAATTY	1 cut(s)	372
XcmI	CCANNNNNNNNNTGG	1 cut(s)	169
XmaJI	CCTAGG	1 cut(s)	302
XmiI	GTMKAC	1 cut(s)	330
XspI	CTAG	2 cut(s)	303, 339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.