Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0021106

Physical Location & Seq

Genomic Coordinates Reverse (-)

62115838 .. 62116254

Gene Structure 417 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030465

Sequence Viewer

CDS Protein Promoter (2k)

Length: 417 bp

ATGGGGAAAGACCAAGCAGCAGTAAATGGGGAAAGATTCGATTCGGCATCTTCCAGAGGATATGTGTATCAAAAAGCCTATGTGGAGTTTTTCTGTTCCAAGGAGACATTGGATGTTCTTATCGAAAAATGCAAGGCTCATCCATCTCTAACCTACATGGCCGTGAACAGACAAGGTAGTTGTTGGATATCTAATCATGATCAGAGTAATATCAATGCAGTTACATGGGGAGTCTTCCCTGGGAAGGAGATAATCGAACGGACTGTCGACGCGACTACGTTTAGGGTTTGGAAGGATGAGGCTTTCGAAATTTGGTCAGAGAAATTACTTGAACAGGGGAACAACTACTTCTTGGTTAGCTTGGTAGATAATGACTACATCCATGGTGACCTTTCCATCATTTTTCCTGATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004489 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 ko01523 map00670 map00720 map01100 map01120 map01200 map01523

Pfam Domains

Protein Families

MTHFR

Protein Analysis

139

Amino Acids

15.82

Weight (kDa)

4.86

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTHFR_C	PF21895	17 - 127	3.6e-25	MTHFR, SAM-binding regulatory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021106)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g02331
rosa_chinensis	RchiOBHm_Chr1g0319671
rosa_laevigata	RLG00000030465
rosa_roxburghii	Rroxscaffold_4G00328220
rosa_samantha	Rh1BG038300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	267
AccII	CGCG	1 cut(s)	272
AcoI	YGGCCR	1 cut(s)	159
AcsI	RAATTY	1 cut(s)	309
AfiI	CCNNNNNNNGG	1 cut(s)	244
AgsI	TTSAA	1 cut(s)	332
AjnI	CCWGG	1 cut(s)	238
AluBI	AGCT	1 cut(s)	360
AluI	AGCT	1 cut(s)	360
Alw26I	GTCTC	1 cut(s)	98
AoxI	GGCC	1 cut(s)	159
ApeKI	GCWGC	1 cut(s)	17
ApoI	RAATTY	1 cut(s)	309
ArsI	GACNNNNNNTTYG	2 cut(s)	249, 281
AsuHPI	GGTGA	1 cut(s)	398
AsuII	TTCGAA	1 cut(s)	306
BbsI	GAAGAC	1 cut(s)	226
BbvI	GCAGC	1 cut(s)	29
BccI	CCATC	2 cut(s)	151, 404
BceAI	ACGGC	1 cut(s)	146
BciT130I	CCWGG	1 cut(s)	240
BclI	TGATCA	1 cut(s)	199
BcoDI	GTCTC	1 cut(s)	98
BisI	GCNGC	1 cut(s)	18
BlsI	GCNGC	1 cut(s)	19
Bme1390I	CCNGG	1 cut(s)	240
BmrFI	CCNGG	1 cut(s)	240
BmsI	GCATC	1 cut(s)	56
BpiI	GAAGAC	1 cut(s)	226
Bpu14I	TTCGAA	1 cut(s)	306
BsaJI	CCNNGG	4 cut(s)	99, 238, 239, 382
Bsc4I	CCNNNNNNNGG	1 cut(s)	244
BseBI	CCWGG	1 cut(s)	240
BseDI	CCNNGG	4 cut(s)	99, 238, 239, 382
BseGI	GGATG	4 cut(s)	118, 139, 301, 378
BseLI	CCNNNNNNNGG	1 cut(s)	244
BseXI	GCAGC	1 cut(s)	29
Bsh1236I	CGCG	1 cut(s)	272
BshFI	GGCC	1 cut(s)	161
BslI	CCNNNNNNNGG	1 cut(s)	244
BsmAI	GTCTC	1 cut(s)	98
BsnI	GGCC	1 cut(s)	161
Bsp119I	TTCGAA	1 cut(s)	306
Bsp143I	GATC	1 cut(s)	199
Bsp19I	CCATGG	1 cut(s)	382
BspANI	GGCC	1 cut(s)	161
BspFNI	CGCG	1 cut(s)	272
BspHI	TCATGA	1 cut(s)	196
BspT104I	TTCGAA	1 cut(s)	306
BssECI	CCNNGG	4 cut(s)	99, 238, 239, 382
BssMI	GATC	1 cut(s)	199
BssT1I	CCWWGG	2 cut(s)	99, 382
Bst2UI	CCWGG	1 cut(s)	240
Bst4CI	ACNGT	1 cut(s)	265
BstBI	TTCGAA	1 cut(s)	306
BstDSI	CCRYGG	1 cut(s)	382
BstEII	GGTNACC	1 cut(s)	386
BstF5I	GGATG	4 cut(s)	118, 139, 301, 378
BstFNI	CGCG	1 cut(s)	272
BstKTI	GATC	1 cut(s)	202
BstMAI	GTCTC	1 cut(s)	98
BstMBI	GATC	1 cut(s)	199
BstNI	CCWGG	1 cut(s)	240
BstPI	GGTNACC	1 cut(s)	386
BstSCI	CCNGG	1 cut(s)	238
BstUI	CGCG	1 cut(s)	272
BstV1I	GCAGC	1 cut(s)	29
BstV2I	GAAGAC	1 cut(s)	226
BsuRI	GGCC	1 cut(s)	161
BtgI	CCRYGG	1 cut(s)	382
BtsCI	GGATG	4 cut(s)	118, 139, 301, 378
CciI	TCATGA	1 cut(s)	196
CseI	GACGC	1 cut(s)	278
CviAII	CATG	4 cut(s)	157, 197, 225, 383
CviJI	RGCY	5 cut(s)	77, 137, 161, 302, 360
CviKI_1	RGCY	5 cut(s)	77, 137, 161, 302, 360
DpnI	GATC	1 cut(s)	201
DpnII	GATC	1 cut(s)	199
EaeI	YGGCCR	1 cut(s)	159
Eco130I	CCWWGG	2 cut(s)	99, 382
Eco32I	GATATC	1 cut(s)	189
Eco91I	GGTNACC	1 cut(s)	386
EcoO65I	GGTNACC	1 cut(s)	386
EcoRII	CCWGG	1 cut(s)	238
EcoRV	GATATC	1 cut(s)	189
EcoT14I	CCWWGG	2 cut(s)	99, 382
ErhI	CCWWGG	2 cut(s)	99, 382
FaeI	CATG	4 cut(s)	160, 200, 228, 386
FaiI	YATR	6 cut(s)	63, 81, 158, 198, 226, 384
FatI	CATG	4 cut(s)	156, 196, 224, 382
FbaI	TGATCA	1 cut(s)	199
FblI	GTMKAC	1 cut(s)	267
Fnu4HI	GCNGC	1 cut(s)	18
FokI	GGATG	4 cut(s)	125, 126, 308, 365
Fsp4HI	GCNGC	1 cut(s)	18
GluI	GCNGC	1 cut(s)	18
HaeIII	GGCC	1 cut(s)	161
HgaI	GACGC	1 cut(s)	278
Hin1II	CATG	4 cut(s)	160, 200, 228, 386
HincII	GTYRAC	1 cut(s)	268
HindII	GTYRAC	1 cut(s)	268
HinfI	GANTC	3 cut(s)	36, 41, 231
HphI	GGTGA	1 cut(s)	398
Hpy166II	GTNNAC	2 cut(s)	166, 268
Hpy188I	TCNGA	2 cut(s)	204, 319
Hpy188III	TCNNGA	3 cut(s)	54, 197, 407
Hpy8I	GTNNAC	2 cut(s)	166, 268
Hpy99I	CGWCG	1 cut(s)	272
HpyAV	CCTTC	2 cut(s)	238, 286
HpyCH4III	ACNGT	1 cut(s)	265
HpyCH4IV	ACGT	1 cut(s)	278
HpyCH4V	TGCA	2 cut(s)	132, 218
HpySE526I	ACGT	1 cut(s)	278
Hsp92II	CATG	4 cut(s)	160, 200, 228, 386
Ksp22I	TGATCA	1 cut(s)	199
Kzo9I	GATC	1 cut(s)	199
LpnPI	CCDG	4 cut(s)	67, 225, 252, 320
Lsp1109I	GCAGC	1 cut(s)	29
LweI	GCATC	1 cut(s)	56
MaeII	ACGT	1 cut(s)	278
MaeIII	GTNAC	2 cut(s)	220, 386
MalI	GATC	1 cut(s)	201
MboI	GATC	1 cut(s)	199
MboII	GAAGA	2 cut(s)	42, 226
MluCI	AATT	2 cut(s)	309, 323
MlyI	GAGTC	1 cut(s)	240
MmeI	TCCRAC	1 cut(s)	164
MnlI	CCTC	2 cut(s)	50, 292
MslI	CAYNNNNRTG	1 cut(s)	161
MspR9I	CCNGG	1 cut(s)	240
MvaI	CCWGG	1 cut(s)	240
MvnI	CGCG	1 cut(s)	272
NcoI	CCATGG	1 cut(s)	382
NdeII	GATC	1 cut(s)	199
NlaIII	CATG	4 cut(s)	160, 200, 228, 386
NmuCI	GTSAC	1 cut(s)	386
NspV	TTCGAA	1 cut(s)	306
PagI	TCATGA	1 cut(s)	196
PasI	CCCWGGG	1 cut(s)	239
PfeI	GAWTC	2 cut(s)	36, 41
PkrI	GCNGC	1 cut(s)	19
PleI	GAGTC	1 cut(s)	239
PpsI	GAGTC	1 cut(s)	239
Psp6I	CCWGG	1 cut(s)	238
PspEI	GGTNACC	1 cut(s)	386
PspGI	CCWGG	1 cut(s)	238
RseI	CAYNNNNRTG	1 cut(s)	161
SalI	GTCGAC	1 cut(s)	266
SatI	GCNGC	1 cut(s)	18
Sau3AI	GATC	1 cut(s)	199
SchI	GAGTC	1 cut(s)	240
ScrFI	CCNGG	1 cut(s)	240
SetI	ASST	5 cut(s)	155, 178, 281, 362, 393
SfaNI	GCATC	1 cut(s)	56
SfuI	TTCGAA	1 cut(s)	306
SmiMI	CAYNNNNRTG	1 cut(s)	161
Sse9I	AATT	2 cut(s)	309, 323
StyD4I	CCNGG	1 cut(s)	238
StyI	CCWWGG	2 cut(s)	99, 382
TaaI	ACNGT	1 cut(s)	265
TaiI	ACGT	1 cut(s)	281
TaqI	TCGA	5 cut(s)	39, 123, 255, 267, 306
TasI	AATT	2 cut(s)	309, 323
TfiI	GAWTC	2 cut(s)	36, 41
TseFI	GTSAC	1 cut(s)	386
TseI	GCWGC	1 cut(s)	17
Tsp45I	GTSAC	1 cut(s)	386
TspGWI	ACGGA	1 cut(s)	274
XapI	RAATTY	1 cut(s)	309
XcmI	CCANNNNNNNNNTGG	1 cut(s)	106
XmiI	GTMKAC	1 cut(s)	267

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.