RchiOBHm_Chr1g0319671

Methylenetetrahydrofolate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0021106
Physical Location & Seq
Forward (+)
7301187 .. 7301747
561 bp
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UTR
Exon/CDS
Intron
PRQ54989

Sequence Viewer

Length: 561 bp
ATGTATTTTTGCCCAAACATCTGCACTGGAAAGTCGATAAGTACAAGCCCTAATTGGTTAGAACAAGACGGGCTTGATCAGCTGCCAGAGACGAGGAAGATCAAGAATGAACTGCTGGAGGAACTTAACAGAAAAGGTTTCATTATCATTAACAGCCAAGCAGCAGTAAATGGGGAAAGATTCGATTCGGCATCTTTTGGAGGATATGTGTATCAAAAAGCCTATGTGGAGTTTTTCTGTTCCAAGGAGACATTGGATGTTCTTATCGAAAAATGTAAGGTTCATCTATCTCTGACCTACATGGCCGTGAACAGACAAGGTAGTTGTTGGATATCTAATCTTGATCAGAGTAATATCAATGCAGTTACATGGGGAGTCTTCCCTAGGAAGGAGATTATCGAGCGGACTGTTGACGCGGCTAGGTTTAGGGTTTGGAAGGATGAGGCTTTCGAAATTTGGTCAGAGAAATTACTTGAACAGGGGAACAACTACTTCTTAGTTAGCTTGGTAGATAATGACTACATCCATGGTGACCTTTCCACCGTTTTTCCTGATTTCTAA

Protein Analysis

186

Amino Acids

21.45

Weight (kDa)

4.92

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTHFR_C PF21895 29 - 175 1.8e-29 MTHFR, SAM-binding regulatory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021106)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02331
rosa_chinensis RchiOBHm_Chr1g0319671
rosa_laevigata RLG00000030465
rosa_roxburghii Rroxscaffold_4G00328220
rosa_samantha Rh1BG038300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 403
AccII CGCG 1 cut(s) 416
AciI CCGC 2 cut(s) 403, 416
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 1 cut(s) 453
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 1 cut(s) 476
AluBI AGCT 2 cut(s) 82, 504
AluI AGCT 2 cut(s) 82, 504
Alw26I GTCTC 2 cut(s) 83, 242
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 82, 161
ApoI RAATTY 1 cut(s) 453
AspA2I CCTAGG 1 cut(s) 383
AsuHPI GGTGA 1 cut(s) 542
AsuII TTCGAA 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 69, 173
BceAI ACGGC 1 cut(s) 290
BclI TGATCA 2 cut(s) 76, 343
BcoDI GTCTC 2 cut(s) 83, 242
BfaI CTAG 2 cut(s) 384, 420
BisI GCNGC 3 cut(s) 83, 162, 417
BlnI CCTAGG 1 cut(s) 383
BlsI GCNGC 3 cut(s) 84, 163, 418
BmsI GCATC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 370
BpmI CTGGAG 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 450
BsaJI CCNNGG 3 cut(s) 243, 383, 526
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse1I ACTGG 1 cut(s) 31
BseDI CCNNGG 3 cut(s) 243, 383, 526
BseGI GGATG 3 cut(s) 262, 445, 522
BseLI CCNNNNNNNGG 1 cut(s) 388
BseNI ACTGG 1 cut(s) 31
BseXI GCAGC 2 cut(s) 69, 173
BsgI GTGCAG 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 2 cut(s) 83, 242
BsmBI CGTCTC 1 cut(s) 83
BsnI GGCC 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 450
Bsp143I GATC 3 cut(s) 76, 99, 343
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 2 cut(s) 403, 416
BspANI GGCC 1 cut(s) 305
BspFNI CGCG 1 cut(s) 416
BspT104I TTCGAA 1 cut(s) 450
BsrBI CCGCTC 1 cut(s) 403
BsrI ACTGG 1 cut(s) 31
BssECI CCNNGG 3 cut(s) 243, 383, 526
BssMI GATC 3 cut(s) 76, 99, 343
BssT1I CCWWGG 3 cut(s) 243, 383, 526
Bst4CI ACNGT 2 cut(s) 409, 544
BstBI TTCGAA 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 496
BstDSI CCRYGG 1 cut(s) 526
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 3 cut(s) 262, 445, 522
BstFNI CGCG 1 cut(s) 416
BstKTI GATC 3 cut(s) 79, 102, 346
BstMAI GTCTC 2 cut(s) 83, 242
BstMBI GATC 3 cut(s) 76, 99, 343
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstPI GGTNACC 1 cut(s) 530
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 2 cut(s) 69, 173
BstV2I GAAGAC 1 cut(s) 370
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 3 cut(s) 262, 445, 522
BtsIMutI CAGTG 1 cut(s) 24
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 42
CviAII CATG 3 cut(s) 301, 369, 527
CviJI RGCY 9 cut(s) 48, 73, 82, 156, 221, 305, 419, 446, 504
CviKI_1 RGCY 9 cut(s) 48, 73, 82, 156, 221, 305, 419, 446, 504
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 1 cut(s) 496
DpnI GATC 3 cut(s) 78, 101, 345
DpnII GATC 3 cut(s) 76, 99, 343
EaeI YGGCCR 1 cut(s) 303
Eco130I CCWWGG 3 cut(s) 243, 383, 526
Eco32I GATATC 1 cut(s) 333
Eco91I GGTNACC 1 cut(s) 530
EcoO65I GGTNACC 1 cut(s) 530
EcoRV GATATC 1 cut(s) 333
EcoT14I CCWWGG 3 cut(s) 243, 383, 526
ErhI CCWWGG 3 cut(s) 243, 383, 526
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 3 cut(s) 304, 372, 530
FaiI YATR 5 cut(s) 207, 225, 302, 370, 528
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FatI CATG 3 cut(s) 300, 368, 526
FbaI TGATCA 2 cut(s) 76, 343
Fnu4HI GCNGC 3 cut(s) 83, 162, 417
FokI GGATG 3 cut(s) 269, 452, 509
Fsp4HI GCNGC 3 cut(s) 83, 162, 417
FspBI CTAG 2 cut(s) 384, 420
GluI GCNGC 3 cut(s) 83, 162, 417
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 1 cut(s) 305
HgaI GACGC 1 cut(s) 422
Hin1II CATG 3 cut(s) 304, 372, 530
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 3 cut(s) 180, 185, 375
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 2 cut(s) 310, 412
Hpy188I TCNGA 3 cut(s) 294, 348, 463
Hpy188III TCNNGA 3 cut(s) 103, 341, 551
Hpy8I GTNNAC 2 cut(s) 310, 412
HpyAV CCTTC 2 cut(s) 382, 430
HpyCH4III ACNGT 2 cut(s) 409, 544
HpyCH4V TGCA 2 cut(s) 24, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 1 cut(s) 496
Hsp92II CATG 3 cut(s) 304, 372, 530
Ksp22I TGATCA 2 cut(s) 76, 343
Kzo9I GATC 3 cut(s) 76, 99, 343
LpnPI CCDG 4 cut(s) 12, 99, 101, 464
Lsp1109I GCAGC 2 cut(s) 69, 173
LweI GCATC 1 cut(s) 200
MaeI CTAG 2 cut(s) 384, 420
MaeIII GTNAC 2 cut(s) 364, 530
MalI GATC 3 cut(s) 78, 101, 345
MbiI CCGCTC 1 cut(s) 403
MboI GATC 3 cut(s) 76, 99, 343
MboII GAAGA 2 cut(s) 109, 370
MluCI AATT 3 cut(s) 52, 453, 467
MlyI GAGTC 1 cut(s) 384
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 4 cut(s) 87, 112, 194, 436
MseI TTAA 2 cut(s) 126, 150
MslI CAYNNNNRTG 1 cut(s) 305
MspA1I CMGCKG 1 cut(s) 82
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 79
NcoI CCATGG 1 cut(s) 526
NdeII GATC 3 cut(s) 76, 99, 343
NlaIII CATG 3 cut(s) 304, 372, 530
NmuCI GTSAC 1 cut(s) 530
NspV TTCGAA 1 cut(s) 450
PfeI GAWTC 2 cut(s) 180, 185
PkrI GCNGC 3 cut(s) 84, 163, 418
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PspEI GGTNACC 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 82
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 2 cut(s) 126, 150
SatI GCNGC 3 cut(s) 83, 162, 417
Sau3AI GATC 3 cut(s) 76, 99, 343
SchI GAGTC 1 cut(s) 384
SetI ASST 8 cut(s) 84, 139, 282, 299, 322, 425, 506, 537
SfaNI GCATC 1 cut(s) 200
SfuI TTCGAA 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 305
Sse9I AATT 3 cut(s) 52, 453, 467
SsiI CCGC 2 cut(s) 403, 416
SspMI CTAG 2 cut(s) 384, 420
StyI CCWWGG 3 cut(s) 243, 383, 526
TaaI ACNGT 2 cut(s) 409, 544
TaqI TCGA 5 cut(s) 35, 183, 267, 399, 450
TasI AATT 3 cut(s) 52, 453, 467
TatI WGTACW 1 cut(s) 41
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 180, 185
Tru1I TTAA 2 cut(s) 126, 150
Tru9I TTAA 2 cut(s) 126, 150
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 2 cut(s) 82, 161
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 3 cut(s) 123, 130, 272
TspRI CASTG 1 cut(s) 31
XapI RAATTY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XmaJI CCTAGG 1 cut(s) 383
XspI CTAG 2 cut(s) 384, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.