FvH4_7g08600

Rgp1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007398
Physical Location & Seq
Reverse (-)
8452506 .. 8463598
11093 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08600.t6

Sequence Viewer

Length: 1299 bp
ATGGAGGCCTCGACACCGGCATTGGTTACCAATCAAATTGTTTCGCCAGGGGCCACGAAATCATATGTGGTACGGTCAGTGCTTCCAAGCATTATACCACCGTCATACAAAGGTGCTACCATTCGGTATATGTACTATGTTCGAAGTACAATGTCTGGACAATGGTTGATATTGGAGAATGCCCATTCTCGTGGAGAGTCAGTTAAAGATTTTCCAGAAATGGAAGCTCGTACTCCATTACAAGTATGGGTCACTCAGAAGACCAGTGGCTTGGGAGCAGAAGAAAGTCAAAGTGATGGGATTGTTCCTTCGTCAACCATGCAAATTGATATGTATTGGAAAGAGATGGATGGTGACTCTGAATGGGTAAAAGGAAATGATGTATATGATGGGATTGAGGATGGCTATGAAAGCTCAAAGGATGAGATCTCATCCGTTTCATCTTACAATCCTATGAAAGAACATATGAACAGAACCTTTGGTAGTTCATTATCATTGCAATCAGCTAGGTCTTCAAACAAGGATACATATCTTGAAAGAGAACGCACAAGTCTATCTTCAAATCTGGCACTTCCACTGCTCTCCGCTGCTGAAGTCGTGTATGATACTGGTGCTGATCTTAAATCGCCCAAAAATTCCTCTGTTGTTGGCTCTCCAAGCAGGCAGCAGAAACTTACAAAATCCCTTCCTTCAGAAGATGAAGCAAGAGCATCTACTTCACCAGCAGCTGGTGCTGCTGAAACCTTAGCATCAGAAGGCTTTATTCGAGGGAGGTCTTACAATATCCGGCTGGACGACAAAGTTCTGCTTAGATTTTCCCCGAAAAATTCTGATTCTAATTATTACTTCAGTGATATGATTGGTGGAACTCTTACTTTCTTTCATGAAGAAGGAGCTAGAAGATGCCTTGAGGTTTCTATAACACTGGAGACTTCAGAAACCATTAGTCGGCGATATGTCCATCCTTCTCGGAAGAATTCCCCTACAATTACAAAGGTTCAGAGTGATCATTACGAGGTTGTTGCTGATTTAGTGCAGACAAGTTTTCTCTTTTCCATTCCTATGGATGGGCCTATGTCTTTTTCCACTCCTCATGTATCTATAAAATGGGTCCTTCGGTTTGAATTTTTTACCACTCCAAAGAATGTTGACTGGACCAGGTACGATCATCCTCTTCTTGTAGAGGGAAGAGACAAAAGTGAGTGGGTTCTTCCTATCGTTGTTCATGCACCTCCAGGTGTGGGACCTAATGGTCAGTCACGAAATGAGAAGTCTCTGCTCGGAATAGTTGGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rgp1

Protein Analysis

433

Amino Acids

47.88

Weight (kDa)

5.62

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rgp1 PF08737 363 - 483 4.8e-13 Rgp1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1251
AccB7I CCANNNNNTGG 1 cut(s) 728
AciI CCGC 1 cut(s) 585
AcsI RAATTY 4 cut(s) 634, 826, 976, 1124
AcuI CTGAAG 4 cut(s) 612, 675, 832, 918
AfaI GTAC 5 cut(s) 72, 134, 148, 232, 1163
AfiI CCNNNNNNNGG 4 cut(s) 728, 948, 1067, 1241
AgsI TTSAA 4 cut(s) 516, 536, 561, 1124
AjnI CCWGG 3 cut(s) 46, 1157, 1234
AluBI AGCT 5 cut(s) 227, 414, 506, 728, 896
AluI AGCT 5 cut(s) 227, 414, 506, 728, 896
Alw26I GTCTC 3 cut(s) 923, 1185, 1278
AlwNI CAGNNNCTG 1 cut(s) 728
AoxI GGCC 3 cut(s) 6, 51, 1070
ApeKI GCWGC 4 cut(s) 587, 664, 725, 734
ApoI RAATTY 4 cut(s) 634, 826, 976, 1124
AspS9I GGNCC 5 cut(s) 51, 1070, 1111, 1155, 1244
AsuHPI GGTGA 2 cut(s) 365, 711
AsuII TTCGAA 1 cut(s) 142
AvaII GGWCC 3 cut(s) 1111, 1155, 1244
BauI CACGAG 1 cut(s) 189
BbsI GAAGAC 2 cut(s) 266, 504
BbvI GCAGC 4 cut(s) 574, 676, 721, 737
BccI CCATC 7 cut(s) 290, 340, 344, 383, 395, 969, 1061
BcgI CGANNNNNNTGC 2 cut(s) 1004, 1038
BciT130I CCWGG 3 cut(s) 48, 1159, 1236
BciVI GTATCC 1 cut(s) 517
BclI TGATCA 1 cut(s) 1006
BcoDI GTCTC 3 cut(s) 923, 1185, 1278
BfaI CTAG 2 cut(s) 507, 897
BfuI GTATCC 1 cut(s) 517
BglII AGATCT 1 cut(s) 426
BisI GCNGC 4 cut(s) 588, 665, 726, 735
BlsI GCNGC 4 cut(s) 589, 666, 727, 736
Bme1390I CCNGG 3 cut(s) 48, 1159, 1236
Bme18I GGWCC 3 cut(s) 1111, 1155, 1244
BmgT120I GGNCC 5 cut(s) 51, 1070, 1111, 1155, 1244
BmiI GGNNCC 3 cut(s) 52, 1112, 1245
BmrFI CCNGG 3 cut(s) 48, 1159, 1236
BmsI GCATC 3 cut(s) 719, 758, 893
BpiI GAAGAC 2 cut(s) 266, 504
BpmI CTGGAG 2 cut(s) 947, 1218
Bpu10I CCTNAGC 1 cut(s) 745
Bpu14I TTCGAA 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 929
BsaBI GATNNNNATC 1 cut(s) 528
BsaJI CCNNGG 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 4 cut(s) 728, 948, 1067, 1241
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 4 cut(s) 264, 613, 930, 1157
Bse3DI GCAATG 1 cut(s) 494
Bse8I GATNNNNATC 1 cut(s) 528
BseBI CCWGG 3 cut(s) 48, 1159, 1236
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 7 cut(s) 355, 406, 427, 431, 961, 1072, 1168
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 4 cut(s) 728, 948, 1067, 1241
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 4 cut(s) 264, 613, 930, 1157
BseRI GAGGAG 1 cut(s) 1080
BseXI GCAGC 4 cut(s) 574, 676, 721, 737
BsgI GTGCAG 1 cut(s) 1055
BshFI GGCC 3 cut(s) 8, 53, 1072
BsiSI CCGG 2 cut(s) 17, 787
BslFI GGGAC 1 cut(s) 1257
BslI CCNNNNNNNGG 4 cut(s) 728, 948, 1067, 1241
BsmAI GTCTC 3 cut(s) 923, 1185, 1278
BsmFI GGGAC 1 cut(s) 1257
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 3 cut(s) 8, 53, 1072
Bsp119I TTCGAA 1 cut(s) 142
Bsp143I GATC 4 cut(s) 426, 616, 1006, 1165
BspACI CCGC 1 cut(s) 585
BspANI GGCC 3 cut(s) 8, 53, 1072
BspCNI CTCAG 1 cut(s) 268
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 3 cut(s) 52, 1112, 1245
BspT104I TTCGAA 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 4 cut(s) 264, 613, 930, 1157
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 4 cut(s) 426, 616, 1006, 1165
BssSI CACGAG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 48, 1159, 1236
Bst4CI ACNGT 2 cut(s) 75, 102
Bst6I CTCTTC 2 cut(s) 1179, 1183
BstAPI GCANNNNNTGC 1 cut(s) 731
BstBI TTCGAA 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 662
BstDEI CTNAG 3 cut(s) 255, 745, 809
BstEII GGTNACC 1 cut(s) 25
BstF5I GGATG 7 cut(s) 355, 406, 427, 431, 961, 1072, 1168
BstKTI GATC 4 cut(s) 429, 619, 1009, 1168
BstMAI GTCTC 3 cut(s) 923, 1185, 1278
BstMBI GATC 4 cut(s) 426, 616, 1006, 1165
BstMWI GCNNNNNNNGC 4 cut(s) 411, 657, 731, 734
BstNI CCWGG 3 cut(s) 48, 1159, 1236
BstPI GGTNACC 1 cut(s) 25
BstSCI CCNGG 3 cut(s) 46, 1157, 1234
BstV1I GCAGC 4 cut(s) 574, 676, 721, 737
BstV2I GAAGAC 2 cut(s) 266, 504
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 3 cut(s) 191, 271, 1063
BstYI RGATCY 1 cut(s) 426
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 3 cut(s) 8, 53, 1072
BtsCI GGATG 7 cut(s) 355, 406, 427, 431, 961, 1072, 1168
BtsI GCAGTG 1 cut(s) 575
BtsIMutI CAGTG 5 cut(s) 84, 271, 575, 856, 923
Cac8I GCNNGC 1 cut(s) 662
CaiI CAGNNNCTG 1 cut(s) 728
CciI TCATGA 1 cut(s) 883
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 5 cut(s) 51, 1070, 1111, 1155, 1244
CsiI ACCWGGT 1 cut(s) 1157
Csp6I GTAC 5 cut(s) 71, 133, 147, 231, 1162
CviAII CATG 4 cut(s) 319, 884, 1094, 1226
CviQI GTAC 5 cut(s) 71, 133, 147, 231, 1162
DdeI CTNAG 3 cut(s) 255, 745, 809
DpnI GATC 4 cut(s) 428, 618, 1008, 1167
DpnII GATC 4 cut(s) 426, 616, 1006, 1165
DrdI GACNNNNNNGTC 1 cut(s) 1251
DseDI GACNNNNNNGTC 1 cut(s) 1251
Eam1104I CTCTTC 2 cut(s) 1179, 1183
EarI CTCTTC 2 cut(s) 1179, 1183
Eco147I AGGCCT 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 1111, 1155, 1244
Eco57I CTGAAG 4 cut(s) 612, 675, 832, 918
Eco91I GGTNACC 1 cut(s) 25
EcoO109I RGGNCCY 2 cut(s) 1111, 1244
EcoO65I GGTNACC 1 cut(s) 25
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 3 cut(s) 46, 1157, 1234
FaeI CATG 4 cut(s) 322, 887, 1097, 1229
FalI AAGNNNNNCTT 4 cut(s) 541, 573, 792, 824
FaqI GGGAC 1 cut(s) 1257
FatI CATG 4 cut(s) 318, 883, 1093, 1225
FauNDI CATATG 2 cut(s) 64, 465
FbaI TGATCA 1 cut(s) 1006
Fnu4HI GCNGC 4 cut(s) 588, 665, 726, 735
FokI GGATG 7 cut(s) 362, 413, 418, 434, 948, 1079, 1155
Fsp4HI GCNGC 4 cut(s) 588, 665, 726, 735
FspBI CTAG 2 cut(s) 507, 897
GluI GCNGC 4 cut(s) 588, 665, 726, 735
GsuI CTGGAG 2 cut(s) 947, 1218
HaeIII GGCC 3 cut(s) 8, 53, 1072
HapII CCGG 2 cut(s) 17, 787
Hin1II CATG 4 cut(s) 322, 887, 1097, 1229
HincII GTYRAC 2 cut(s) 315, 1150
HindII GTYRAC 2 cut(s) 315, 1150
HinfI GANTC 3 cut(s) 197, 356, 833
HpaII CCGG 2 cut(s) 17, 787
HphI GGTGA 2 cut(s) 365, 711
Hpy166II GTNNAC 2 cut(s) 315, 1150
Hpy188I TCNGA 9 cut(s) 258, 361, 694, 754, 832, 937, 972, 1002, 1283
Hpy188III TCNNGA 5 cut(s) 156, 215, 533, 884, 1260
Hpy8I GTNNAC 2 cut(s) 315, 1150
HpyAV CCTTC 7 cut(s) 318, 695, 699, 749, 884, 975, 1124
HpyCH4III ACNGT 2 cut(s) 75, 102
HpyCH4V TGCA 5 cut(s) 322, 499, 1036, 1229, 1295
HpyF10VI GCNNNNNNNGC 4 cut(s) 411, 657, 731, 734
HpyF3I CTNAG 3 cut(s) 255, 745, 809
Hsp92II CATG 4 cut(s) 322, 887, 1097, 1229
Ksp22I TGATCA 1 cut(s) 1006
Kzo9I GATC 4 cut(s) 426, 616, 1006, 1165
LmnI GCTCC 2 cut(s) 275, 893
Lsp1109I GCAGC 4 cut(s) 574, 676, 721, 737
LweI GCATC 3 cut(s) 719, 758, 893
MabI ACCWGGT 1 cut(s) 1157
MaeI CTAG 2 cut(s) 507, 897
MaeIII GTNAC 4 cut(s) 25, 250, 353, 1257
MalI GATC 4 cut(s) 428, 618, 1008, 1167
MboI GATC 4 cut(s) 426, 616, 1006, 1165
MflI RGATCY 1 cut(s) 426
MluCI AATT 8 cut(s) 36, 324, 634, 826, 838, 976, 987, 1124
MlyI GAGTC 2 cut(s) 206, 350
MseI TTAA 2 cut(s) 204, 621
MslI CAYNNNNRTG 2 cut(s) 189, 1061
MspA1I CMGCKG 2 cut(s) 587, 728
MspI CCGG 2 cut(s) 17, 787
MspR9I CCNGG 3 cut(s) 48, 1159, 1236
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 3 cut(s) 48, 1159, 1236
MwoI GCNNNNNNNGC 4 cut(s) 411, 657, 731, 734
NdeI CATATG 2 cut(s) 64, 465
NdeII GATC 4 cut(s) 426, 616, 1006, 1165
NlaIII CATG 4 cut(s) 322, 887, 1097, 1229
NlaIV GGNNCC 3 cut(s) 52, 1112, 1245
NmuCI GTSAC 3 cut(s) 250, 353, 1257
NspV TTCGAA 1 cut(s) 142
PagI TCATGA 1 cut(s) 883
PceI AGGCCT 1 cut(s) 8
PctI GAATGC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 833
PflMI CCANNNNNTGG 1 cut(s) 728
PkrI GCNGC 4 cut(s) 589, 666, 727, 736
PleI GAGTC 2 cut(s) 205, 350
PpsI GAGTC 2 cut(s) 205, 350
PpuMI RGGWCCY 2 cut(s) 1111, 1244
Psp5II RGGWCCY 2 cut(s) 1111, 1244
Psp6I CCWGG 3 cut(s) 46, 1157, 1234
PspEI GGTNACC 1 cut(s) 25
PspGI CCWGG 3 cut(s) 46, 1157, 1234
PspN4I GGNNCC 3 cut(s) 52, 1112, 1245
PspPI GGNCC 5 cut(s) 51, 1070, 1111, 1155, 1244
PspPPI RGGWCCY 2 cut(s) 1111, 1244
PstNI CAGNNNCTG 1 cut(s) 728
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 5 cut(s) 72, 134, 148, 232, 1163
RsaNI GTAC 5 cut(s) 71, 133, 147, 231, 1162
RseI CAYNNNNRTG 2 cut(s) 189, 1061
SaqAI TTAA 2 cut(s) 204, 621
SatI GCNGC 4 cut(s) 588, 665, 726, 735
Sau3AI GATC 4 cut(s) 426, 616, 1006, 1165
Sau96I GGNCC 5 cut(s) 51, 1070, 1111, 1155, 1244
SchI GAGTC 2 cut(s) 206, 350
ScrFI CCNGG 3 cut(s) 48, 1159, 1236
SexAI ACCWGGT 1 cut(s) 1157
SfaNI GCATC 3 cut(s) 719, 758, 893
SfuI TTCGAA 1 cut(s) 142
SinI GGWCC 3 cut(s) 1111, 1155, 1244
SmiMI CAYNNNNRTG 2 cut(s) 189, 1061
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
Sse9I AATT 8 cut(s) 36, 324, 634, 826, 838, 976, 987, 1124
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 1 cut(s) 585
SspMI CTAG 2 cut(s) 507, 897
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 46, 1157, 1234
TaaI ACNGT 2 cut(s) 75, 102
TaqI TCGA 3 cut(s) 11, 142, 766
TasI AATT 8 cut(s) 36, 324, 634, 826, 838, 976, 987, 1124
TatI WGTACW 2 cut(s) 132, 146
TfiI GAWTC 1 cut(s) 833
Tru1I TTAA 2 cut(s) 204, 621
Tru9I TTAA 2 cut(s) 204, 621
TscAI CASTG 5 cut(s) 84, 271, 582, 856, 930
TseFI GTSAC 3 cut(s) 250, 353, 1257
TseI GCWGC 4 cut(s) 587, 664, 725, 734
Tsp45I GTSAC 3 cut(s) 250, 353, 1257
TspDTI ATGAA 9 cut(s) 423, 429, 470, 477, 482, 714, 872, 900, 1214
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 5 cut(s) 84, 271, 582, 856, 930
Van91I CCANNNNNTGG 1 cut(s) 728
VpaK11BI GGWCC 3 cut(s) 1111, 1155, 1244
XapI RAATTY 4 cut(s) 634, 826, 976, 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 243
XspI CTAG 2 cut(s) 507, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.