MD02G1226800.v1.1

Rgp1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007398
Physical Location & Seq
Forward (+)
26718087 .. 26725826
7740 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1226800.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGTTGCCGCGCAGGTTATCCGCCATTTTCGGAGGCCTCGGTTCGAACGCCAAGACAGTGACCAGCGAGCTCTTTCCGACTCTCAAGCTCCAAACCGACAAGGAAGTCTACAGGCCCGGCGATCCCATCCTCATCACCATCGAGATTTCGAACCCTAGCAGCAATGACGGTTCGGCGTACTCGCTCTTGATCGAGCGCCTAGGGTTTGAGATTAAGGGGATTGAGAAGCTCGATTCTCAGTGGTTCGCCACGCAGAAACCGACGGCTGGATCCAGACAGAGGAGAGGTGAATATGTGTTCATGGAGTGCTCGACTCCAGCCCTGGTTACCAATCAGATTGTTTCACCCGGGGGTACGAAATCATATGTGGTGCGGTCGTTGCTGGCCCCCGTTATACCACCATCGTACAAAGGTGCTACCCTTCGATATATGTACTATGTTAGAAGTACAATGTCTGGACAATGGCTGATATTGGAGAATGCCCACTCTCGTGGAGAATCAGTGAAAGATTTTCCTGAAATGGAAGCTCGTGTTCCAGTACTAGTATGGGTCACTCAGAAAACCAGTGGATTGGGAATGGAAGGTCTTACAGATGGGATTGTACCTTCTGTAACCATCCAAATGGATATGTATTGGAAAGAGATGGATGGGGACTCTGATTGGGTGAAAGCAAACGAAACATATGATGGGGTTGAAGAAGGGTATGAAAGCTCAAGGGATGAGATCTCATCCGTTTCTTCATACAATCCCATGAAAGAACATTTAAACAGAACATTCGGAAGTTCACTATCCTTGCAGTCCCAACGGTCTTCAAATAAGGATAATGTCTATATTGAAGGAGAACGTACAAGTTTATCTTCAAATCTTGCAATCCCACGGCTCTCTATGGCTGAAGTCTTATACGATACTGGTGCTAATCTTTCATTGCCCCTGATTTCATCTGCTATTGGCTCTCCAAGCCAGCAGCAGAATCTCACAAAGCAACTTTCTTTGGATGATGAAGCAAGGCCATCTTCTTCACCAGCATCTGGAGCAGTTGAAACCTTAGCATCAGAGGGCTTCATTCGAGGGAGGTCATACAATATCCGGCTGGATGACCGAGTTCTGCTTAGATGTTCCCCAAAAAATTCTGATTCCAATTATTACCTCAGTGATATGATTGGTGGAACTCTTACTTTCTTTCATGAAGAAGGAGCTAGAAGATGCCTTGAGGTTTCTATAACATTGGAGATTTCAGAAACTATTAGCCGGCGCTTTGTGCATCCTTCTCGGAGGAATTCTCCCACAATTACAAAGATCCAGAGTGATCATTATGAGGTTGTTGCGGACTTGGTGCAGACAAGTTTTCTTTTCTCCATTCCCATGGATGGGCCTATGTCTTTTTCCACTCCCCATGTTTCTGTACAATGGGAACTTCGATTTGAATTTTTTACCACTCCGAAGAATGTGGATTGGACCAGGTACGAGCATCCTCTTTTTATAGAGGGTAGAGACAAAAGCGAGTGGGTTCTGCCAATCACCGTGCATGCACCTCCAGCTGTGGGACCCACTGGTCACGCCCGAAATGAGAAGTCTCTCTTGGGAAACCTTGTGGGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rgp1

Protein Analysis

534

Amino Acids

59.26

Weight (kDa)

5.56

Isoelectric Point (pI)

52.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rgp1 PF08737 114 - 478 8.7e-15 Rgp1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 104, 1551
Acc36I ACCTGC 1 cut(s) 3
AccB1I GGYRCC 1 cut(s) 1595
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 10
AciI CCGC 4 cut(s) 8, 21, 373, 1325
AclWI GGATC 4 cut(s) 116, 264, 277, 1291
AcsI RAATTY 3 cut(s) 1126, 1276, 1424
AcuI CTGAAG 1 cut(s) 912
AfiI CCNNNNNNNGG 6 cut(s) 266, 279, 1028, 1367, 1368, 1541
AgsI TTSAA 6 cut(s) 695, 813, 836, 861, 1040, 1424
AhlI ACTAGT 1 cut(s) 541
AjnI CCWGG 2 cut(s) 321, 1457
AjuI GAANNNNNNNTTGG 2 cut(s) 1562, 1594
AleI CACNNNNGTG 1 cut(s) 489
AluBI AGCT 7 cut(s) 70, 88, 229, 527, 711, 1196, 1538
AluI AGCT 7 cut(s) 70, 88, 229, 527, 711, 1196, 1538
Alw21I GWGCWC 2 cut(s) 72, 311
Alw26I GTCTC 2 cut(s) 1485, 1578
AlwI GGATC 4 cut(s) 116, 264, 277, 1291
AlwNI CAGNNNCTG 1 cut(s) 1028
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 5 cut(s) 34, 113, 384, 1007, 1370
ApeKI GCWGC 2 cut(s) 159, 964
ApoI RAATTY 3 cut(s) 1126, 1276, 1424
AspA2I CCTAGG 1 cut(s) 199
AspLEI GCGC 3 cut(s) 12, 198, 1254
AspS9I GGNCC 5 cut(s) 114, 385, 1370, 1455, 1544
AsuC2I CCSGG 3 cut(s) 117, 348, 349
AsuHPI GGTGA 6 cut(s) 127, 299, 336, 676, 1011, 1510
AsuII TTCGAA 2 cut(s) 44, 149
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 2 cut(s) 1455, 1544
AvrII CCTAGG 1 cut(s) 199
BamHI GGATCC 1 cut(s) 269
BanI GGYRCC 1 cut(s) 1595
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 2 cut(s) 489, 528
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 2 cut(s) 72, 311
BbvI GCAGC 2 cut(s) 171, 976
BceAI ACGGC 2 cut(s) 279, 893
BcgI CGANNNNNNTGC 4 cut(s) 893, 927, 1250, 1284
BciT130I CCWGG 2 cut(s) 323, 1459
BclI TGATCA 1 cut(s) 1306
BcnI CCSGG 3 cut(s) 117, 348, 349
BcoDI GTCTC 2 cut(s) 1485, 1578
BcuI ACTAGT 1 cut(s) 541
BfaI CTAG 4 cut(s) 156, 200, 542, 1197
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 2 cut(s) 199, 1255
BfuAI ACCTGC 1 cut(s) 3
BglII AGATCT 1 cut(s) 723
BisI GCNGC 3 cut(s) 8, 160, 965
BlnI CCTAGG 1 cut(s) 199
BlsI GCNGC 3 cut(s) 9, 161, 966
BmcAI AGTACT 1 cut(s) 540
Bme1390I CCNGG 5 cut(s) 117, 323, 348, 349, 1459
Bme18I GGWCC 2 cut(s) 1455, 1544
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 5 cut(s) 114, 385, 1370, 1455, 1544
BmiI GGNNCC 5 cut(s) 271, 387, 1545, 1546, 1597
BmrFI CCNGG 5 cut(s) 117, 323, 348, 349, 1459
BmsI GCATC 5 cut(s) 1034, 1058, 1193, 1270, 1477
BpiI GAAGAC 1 cut(s) 801
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 3 cut(s) 300, 1050, 1518
Bpu10I CCTNAGC 1 cut(s) 1045
Bpu14I TTCGAA 2 cut(s) 44, 149
BpuEI CTTGAG 3 cut(s) 68, 697, 1229
BpuMI CCSGG 3 cut(s) 117, 348, 349
BsaJI CCNNGG 7 cut(s) 37, 199, 321, 347, 348, 875, 1362
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 6 cut(s) 266, 279, 1028, 1367, 1368, 1541
Bse118I RCCGGY 1 cut(s) 1248
Bse1I ACTGG 4 cut(s) 536, 564, 913, 1555
Bse3DI GCAATG 2 cut(s) 169, 923
BseBI CCWGG 2 cut(s) 323, 1459
BseDI CCNNGG 7 cut(s) 37, 199, 321, 347, 348, 875, 1362
BseLI CCNNNNNNNGG 6 cut(s) 266, 279, 1028, 1367, 1368, 1541
BseMI GCAATG 2 cut(s) 169, 923
BseMII CTCAG 3 cut(s) 251, 569, 1162
BseNI ACTGG 4 cut(s) 536, 564, 913, 1555
BseRI GAGGAG 1 cut(s) 295
BseXI GCAGC 2 cut(s) 171, 976
BsgI GTGCAG 1 cut(s) 1355
Bsh1236I CGCG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 377
BshFI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BshNI GGYRCC 1 cut(s) 1595
BsiEI CGRYCG 1 cut(s) 377
BsiHKAI GWGCWC 2 cut(s) 72, 311
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 4 cut(s) 117, 348, 1087, 1249
BslFI GGGAC 3 cut(s) 665, 784, 1557
BslI CCNNNNNNNGG 6 cut(s) 266, 279, 1028, 1367, 1368, 1541
BsmAI GTCTC 2 cut(s) 1485, 1578
BsmFI GGGAC 3 cut(s) 665, 784, 1557
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BsoBI CYCGRG 1 cut(s) 347
Bsp119I TTCGAA 2 cut(s) 44, 149
Bsp1286I GDGCHC 2 cut(s) 72, 311
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 4 cut(s) 8, 21, 373, 1325
BspANI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BspCNI CTCAG 3 cut(s) 250, 568, 1161
BspFNI CGCG 1 cut(s) 10
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 5 cut(s) 271, 387, 1545, 1546, 1597
BspMI ACCTGC 1 cut(s) 3
BspPI GGATC 4 cut(s) 116, 264, 277, 1291
BspT104I TTCGAA 2 cut(s) 44, 149
BspT107I GGYRCC 1 cut(s) 1595
BsrDI GCAATG 2 cut(s) 169, 923
BsrFI RCCGGY 1 cut(s) 1248
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 4 cut(s) 536, 564, 913, 1555
BssAI RCCGGY 1 cut(s) 1248
BssECI CCNNGG 7 cut(s) 37, 199, 321, 347, 348, 875, 1362
BssMI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
BssSI CACGAG 2 cut(s) 489, 528
BssT1I CCWWGG 2 cut(s) 199, 1362
Bst2BI CACGAG 2 cut(s) 489, 528
Bst2UI CCWGG 2 cut(s) 323, 1459
Bst4CI ACNGT 4 cut(s) 58, 170, 807, 1522
BstAUI TGTACA 1 cut(s) 1402
BstBI TTCGAA 2 cut(s) 44, 149
BstC8I GCNNGC 5 cut(s) 68, 384, 962, 1250, 1527
BstDEI CTNAG 5 cut(s) 237, 555, 1045, 1109, 1148
BstDSI CCRYGG 2 cut(s) 875, 1362
BstEII GGTNACC 1 cut(s) 325
BstFNI CGCG 1 cut(s) 10
BstH2I RGCGCY 2 cut(s) 199, 1255
BstHHI GCGC 3 cut(s) 12, 198, 1254
BstKTI GATC 6 cut(s) 124, 192, 272, 726, 1299, 1309
BstMAI GTCTC 2 cut(s) 1485, 1578
BstMBI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
BstMCI CGRYCG 1 cut(s) 377
BstMWI GCNNNNNNNGC 5 cut(s) 379, 957, 1031, 1258, 1535
BstNI CCWGG 2 cut(s) 323, 1459
BstNSI RCATGY 1 cut(s) 1529
BstPI GGTNACC 1 cut(s) 325
BstSCI CCNGG 5 cut(s) 115, 321, 346, 347, 1457
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 171, 976
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 3 cut(s) 269, 723, 1296
BstXI CCANNNNNNTGG 4 cut(s) 491, 571, 622, 1363
BstYI RGATCY 3 cut(s) 269, 723, 1296
BsuRI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BtgI CCRYGG 2 cut(s) 875, 1362
BtsIMutI CAGTG 6 cut(s) 63, 245, 507, 571, 1156, 1548
BveI ACCTGC 1 cut(s) 3
Cac8I GCNNGC 5 cut(s) 68, 384, 962, 1250, 1527
CaiI CAGNNNCTG 1 cut(s) 1028
CciI TCATGA 1 cut(s) 1183
CfoI GCGC 3 cut(s) 12, 198, 1254
Cfr10I RCCGGY 1 cut(s) 1248
Cfr13I GGNCC 5 cut(s) 114, 385, 1370, 1455, 1544
Cfr9I CCCGGG 1 cut(s) 347
CsiI ACCWGGT 1 cut(s) 1457
CviAII CATG 6 cut(s) 301, 751, 1184, 1363, 1394, 1526
DdeI CTNAG 5 cut(s) 237, 555, 1045, 1109, 1148
DpnI GATC 6 cut(s) 123, 191, 271, 725, 1298, 1308
DpnII GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
DraI TTTAAA 1 cut(s) 765
DrdI GACNNNNNNGTC 2 cut(s) 104, 1551
DseDI GACNNNNNNGTC 2 cut(s) 104, 1551
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 2 cut(s) 199, 1362
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 1455, 1544
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 912
Eco88I CYCGRG 1 cut(s) 347
Eco91I GGTNACC 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 1544
EcoO65I GGTNACC 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 2 cut(s) 321, 1457
EcoT14I CCWWGG 2 cut(s) 199, 1362
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 2 cut(s) 199, 1362
FaeI CATG 6 cut(s) 304, 754, 1187, 1366, 1397, 1529
FalI AAGNNNNNCTT 6 cut(s) 841, 873, 997, 1029, 1562, 1594
FaqI GGGAC 3 cut(s) 665, 784, 1557
FatI CATG 6 cut(s) 300, 750, 1183, 1362, 1393, 1525
FauNDI CATATG 2 cut(s) 364, 682
FbaI TGATCA 1 cut(s) 1306
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 3 cut(s) 8, 160, 965
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 3 cut(s) 8, 160, 965
FspBI CTAG 4 cut(s) 156, 200, 542, 1197
GlaI GCGC 3 cut(s) 11, 197, 1253
GluI GCNGC 3 cut(s) 8, 160, 965
GsuI CTGGAG 3 cut(s) 300, 1050, 1518
HaeII RGCGCY 2 cut(s) 199, 1255
HaeIII GGCC 5 cut(s) 36, 115, 386, 1009, 1372
HapII CCGG 4 cut(s) 117, 348, 1087, 1249
HhaI GCGC 3 cut(s) 12, 198, 1254
Hin1II CATG 6 cut(s) 304, 754, 1187, 1366, 1397, 1529
Hin6I GCGC 3 cut(s) 10, 196, 1252
HinP1I GCGC 3 cut(s) 10, 196, 1252
HinfI GANTC 7 cut(s) 79, 233, 313, 497, 653, 970, 1133
HpaII CCGG 4 cut(s) 117, 348, 1087, 1249
HphI GGTGA 6 cut(s) 127, 299, 336, 676, 1011, 1510
Hpy166II GTNNAC 2 cut(s) 109, 785
Hpy188III TCNNGA 8 cut(s) 142, 187, 273, 456, 515, 1029, 1184, 1300
Hpy8I GTNNAC 2 cut(s) 109, 785
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 7 cut(s) 431, 575, 615, 692, 830, 1184, 1275
HpyCH4III ACNGT 4 cut(s) 58, 170, 807, 1522
HpyCH4IV ACGT 1 cut(s) 844
HpyCH4V TGCA 6 cut(s) 796, 869, 1261, 1336, 1525, 1529
HpyF10VI GCNNNNNNNGC 5 cut(s) 379, 957, 1031, 1258, 1535
HpyF3I CTNAG 5 cut(s) 237, 555, 1045, 1109, 1148
HpySE526I ACGT 1 cut(s) 844
Hsp92II CATG 6 cut(s) 304, 754, 1187, 1366, 1397, 1529
HspAI GCGC 3 cut(s) 10, 196, 1252
KflI GGGWCCC 1 cut(s) 1544
KroI GCCGGC 1 cut(s) 1248
KroNI GCCGGC 1 cut(s) 1250
Ksp22I TGATCA 1 cut(s) 1306
Kzo9I GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
LmnI GCTCC 3 cut(s) 93, 1031, 1193
Lsp1109I GCAGC 2 cut(s) 171, 976
LweI GCATC 5 cut(s) 1034, 1058, 1193, 1270, 1477
MabI ACCWGGT 1 cut(s) 1457
MaeI CTAG 4 cut(s) 156, 200, 542, 1197
MaeII ACGT 1 cut(s) 844
MaeIII GTNAC 5 cut(s) 58, 325, 550, 610, 1553
MalI GATC 6 cut(s) 123, 191, 271, 725, 1298, 1308
MboI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
MboII GAAGA 9 cut(s) 707, 729, 801, 849, 1005, 1008, 1199, 1212, 1453
MflI RGATCY 3 cut(s) 269, 723, 1296
MhlI GDGCHC 2 cut(s) 72, 311
MluCI AATT 5 cut(s) 1126, 1138, 1276, 1287, 1424
MlyI GAGTC 3 cut(s) 73, 307, 647
MmeI TCCRAC 1 cut(s) 101
MroNI GCCGGC 1 cut(s) 1248
MseI TTAA 2 cut(s) 213, 764
MslI CAYNNNNRTG 3 cut(s) 489, 620, 1361
MspA1I CMGCKG 1 cut(s) 1538
MspI CCGG 4 cut(s) 117, 348, 1087, 1249
MspR9I CCNGG 5 cut(s) 117, 323, 348, 349, 1459
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 2 cut(s) 323, 1459
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 5 cut(s) 379, 957, 1031, 1258, 1535
NaeI GCCGGC 1 cut(s) 1250
NciI CCSGG 3 cut(s) 117, 348, 349
NcoI CCATGG 1 cut(s) 1362
NdeI CATATG 2 cut(s) 364, 682
NdeII GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
NgoMIV GCCGGC 1 cut(s) 1248
NlaIII CATG 6 cut(s) 304, 754, 1187, 1366, 1397, 1529
NlaIV GGNNCC 5 cut(s) 271, 387, 1545, 1546, 1597
NmuCI GTSAC 3 cut(s) 58, 550, 1553
NspI RCATGY 1 cut(s) 1529
NspV TTCGAA 2 cut(s) 44, 149
OliI CACNNNNGTG 1 cut(s) 489
PaeI GCATGC 1 cut(s) 1529
PagI TCATGA 1 cut(s) 1183
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PctI GAATGC 1 cut(s) 484
PdiI GCCGGC 1 cut(s) 1250
PfeI GAWTC 4 cut(s) 233, 497, 970, 1133
PflMI CCANNNNNTGG 1 cut(s) 1028
PkrI GCNGC 3 cut(s) 9, 161, 966
PleI GAGTC 3 cut(s) 73, 307, 647
PpsI GAGTC 3 cut(s) 73, 307, 647
PpuMI RGGWCCY 1 cut(s) 1544
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 1544
Psp6I CCWGG 2 cut(s) 321, 1457
PspEI GGTNACC 1 cut(s) 325
PspGI CCWGG 2 cut(s) 321, 1457
PspN4I GGNNCC 5 cut(s) 271, 387, 1545, 1546, 1597
PspPI GGNCC 5 cut(s) 114, 385, 1370, 1455, 1544
PspPPI RGGWCCY 1 cut(s) 1544
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 3 cut(s) 269, 723, 1296
PvuII CAGCTG 1 cut(s) 1538
RseI CAYNNNNRTG 3 cut(s) 489, 620, 1361
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 2 cut(s) 213, 764
SatI GCNGC 3 cut(s) 8, 160, 965
Sau3AI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
Sau96I GGNCC 5 cut(s) 114, 385, 1370, 1455, 1544
ScaI AGTACT 1 cut(s) 540
SchI GAGTC 3 cut(s) 73, 307, 647
ScrFI CCNGG 5 cut(s) 117, 323, 348, 349, 1459
SduI GDGCHC 2 cut(s) 72, 311
SexAI ACCWGGT 1 cut(s) 1457
SfaNI GCATC 5 cut(s) 1034, 1058, 1193, 1270, 1477
SfcI CTRYAG 1 cut(s) 109
SfuI TTCGAA 2 cut(s) 44, 149
SinI GGWCC 2 cut(s) 1455, 1544
SmaI CCCGGG 1 cut(s) 349
SmiMI CAYNNNNRTG 3 cut(s) 489, 620, 1361
SmlI CTYRAG 3 cut(s) 83, 712, 1208
SmoI CTYRAG 3 cut(s) 83, 712, 1208
SpeI ACTAGT 1 cut(s) 541
SphI GCATGC 1 cut(s) 1529
Sse9I AATT 5 cut(s) 1126, 1138, 1276, 1287, 1424
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 4 cut(s) 8, 21, 373, 1325
SspMI CTAG 4 cut(s) 156, 200, 542, 1197
SstI GAGCTC 1 cut(s) 72
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 5 cut(s) 115, 321, 346, 347, 1457
StyI CCWWGG 2 cut(s) 199, 1362
TaaI ACNGT 4 cut(s) 58, 170, 807, 1522
TaiI ACGT 1 cut(s) 847
TaqI TCGA 9 cut(s) 44, 141, 149, 192, 231, 311, 424, 1066, 1417
TaqII GACCGA 1 cut(s) 1113
TasI AATT 5 cut(s) 1126, 1138, 1276, 1287, 1424
TatI WGTACW 4 cut(s) 432, 446, 538, 1402
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 4 cut(s) 233, 497, 970, 1133
Tru1I TTAA 2 cut(s) 213, 764
Tru9I TTAA 2 cut(s) 213, 764
TscAI CASTG 6 cut(s) 63, 245, 507, 571, 1156, 1555
TseFI GTSAC 3 cut(s) 58, 550, 1553
TseI GCWGC 2 cut(s) 159, 964
Tsp45I GTSAC 3 cut(s) 58, 550, 1553
TspGWI ACGGA 1 cut(s) 721
TspMI CCCGGG 1 cut(s) 347
TspRI CASTG 6 cut(s) 63, 245, 507, 571, 1156, 1555
Van91I CCANNNNNTGG 1 cut(s) 1028
VpaK11BI GGWCC 2 cut(s) 1455, 1544
XapI RAATTY 3 cut(s) 1126, 1276, 1424
XceI RCATGY 1 cut(s) 1529
XcmI CCANNNNNNNNNTGG 1 cut(s) 543
XmaI CCCGGG 1 cut(s) 347
XmaJI CCTAGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 4 cut(s) 156, 200, 542, 1197
ZrmI AGTACT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.