FvH4_7g20701

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2 ). Among the enzymes to be stimulated by the calmodulin-Ca(2 ) complex are a number of protein kinases and phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0018478
Physical Location & Seq
Forward (+)
16778355 .. 16779084
730 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20701.t1

Sequence Viewer

Length: 615 bp
ATGTTCTTCTCGCAAACCCTACGTCGAACGACGACCACGGCGGCCACGGCGTCGGCCTATCAGCTCTCCGATGATGAGGCGGCGGAATTCAAGCAGGCTTTTAGCCTCTTGGATAAGGACGGCGACGGCTCCATCACCACCAACGAGCTCGGTACTGTGATGCGCTCACTCGGTCTGAAGCCAACTGAAGAAGAGCTGGAGGGGATGATCGAGGAGGTGGACGCAGATGGGAACGGTAGGGTTGATCTACAAGAGTTCCTTAGCCTAATGGCCCGGAAGATGCAAGACAATCTCTCAGAGAGACAACTCAGGGAAGCGTTTAAGGTTTTCGATAAGGACCAGAATGGATTCATTTCTGCCGATGAGCTTCGTCATGTTATGACCAGCCTCGGCGAGAACCTGATGGATGAGCAGGTTGAGGTGATGCTTCATGAGGCTGATATTGACGGCGATGGACAGATCAATTATGAGGAGTTTGTGAAGGTCATGTTGGCCAAGAAAGTACATAGCCGGAGCCTCGGGCATAGTAGCAGCATGGGTTCAACTAGATCAGAGGGTCACAAAACTAAGAGACTCCGTTGTAGACAGTGTATACGCCAATGTTTCTACCTCTAA

Protein Analysis

205

Amino Acids

23.06

Weight (kDa)

4.97

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 29 - 57 9.8e-08 EF hand domain
EF-hand_6 PF13405 29 - 58 3.6e-08 EF-hand domain
EF-hand_7 PF13499 30 - 90 1.2e-16 EF-hand domain pair
EF-hand_9 PF14658 32 - 92 1.2e-06 EF-hand domain
EF-hand_8 PF13833 42 - 92 3.4e-08 EF-hand domain pair
EF-hand_1 PF00036 65 - 92 1.5e-06 EF hand domain
EF-hand_7 PF13499 101 - 163 6.8e-19 EF-hand domain pair
EF-hand_6 PF13405 102 - 131 4.7e-10 EF-hand domain
EF-hand_1 PF00036 103 - 130 3e-09 EF hand domain
EF-hand_5 PF13202 103 - 127 9.6e-07 EF hand
EF-hand_8 PF13833 115 - 163 7.4e-10 EF-hand domain pair
EF-hand_1 PF00036 139 - 163 9.7e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018478)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20701
rosa_chinensis RchiOBHm_Chr1g0364941
rosa_laevigata RLG00000027457
rosa_multiflora Rmu_ssc0000112.1_g000025
rosa_roxburghii Rroxscaffold_4G00291660
rosa_rugosa Rorug01G0318600
rosa_samantha Rh1BG288600 Rh1CG305300 Rh1DG320200
rosa_wichuraiana Rw1G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 403
AccI GTMKAC 2 cut(s) 583, 592
AciI CCGC 3 cut(s) 41, 80, 83
AcoI YGGCCR 2 cut(s) 42, 492
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 2 cut(s) 197, 207
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 2 cut(s) 154, 504
AgsI TTSAA 2 cut(s) 91, 543
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AluBI AGCT 4 cut(s) 64, 148, 196, 367
AluI AGCT 4 cut(s) 64, 148, 196, 367
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 2 cut(s) 295, 565
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 4 cut(s) 42, 54, 270, 492
ApeKI GCWGC 1 cut(s) 531
ApoI RAATTY 1 cut(s) 86
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
Asp700I GAANNNNTTC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 271, 337
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 127, 433
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 337
BalI TGGCCA 1 cut(s) 494
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 543
BccI CCATC 4 cut(s) 140, 221, 397, 446
BceAI ACGGC 5 cut(s) 54, 63, 136, 142, 463
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 2 cut(s) 295, 565
BfaI CTAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 403
BisI GCNGC 3 cut(s) 42, 81, 532
BlsI GCNGC 3 cut(s) 43, 82, 533
Bme1390I CCNGG 1 cut(s) 274
Bme18I GGWCC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 271, 337
BmiI GGNNCC 2 cut(s) 130, 515
BmrFI CCNGG 1 cut(s) 274
BmsI GCATC 3 cut(s) 150, 270, 414
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 4 cut(s) 36, 45, 388, 517
BseDI CCNNGG 4 cut(s) 36, 45, 388, 517
BseGI GGATG 2 cut(s) 210, 412
BseMII CTCAG 2 cut(s) 309, 322
BseRI GAGGAG 2 cut(s) 227, 485
BseXI GCAGC 1 cut(s) 543
BshFI GGCC 4 cut(s) 44, 56, 272, 494
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 2 cut(s) 274, 511
BsmAI GTCTC 2 cut(s) 295, 565
BsnI GGCC 4 cut(s) 44, 56, 272, 494
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 207, 244, 459, 548
BspACI CCGC 3 cut(s) 41, 80, 83
BspANI GGCC 4 cut(s) 44, 56, 272, 494
BspCNI CTCAG 2 cut(s) 308, 321
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 130, 515
BspMI ACCTGC 1 cut(s) 403
BspQI GCTCTTC 1 cut(s) 186
BssECI CCNNGG 4 cut(s) 36, 45, 388, 517
BssMI GATC 4 cut(s) 207, 244, 459, 548
BssNAI GTATAC 1 cut(s) 593
BssNI GRCGYC 1 cut(s) 50
Bst1107I GTATAC 1 cut(s) 593
Bst4CI ACNGT 3 cut(s) 157, 236, 588
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 4 cut(s) 260, 295, 308, 567
BstDSI CCRYGG 2 cut(s) 36, 45
BstF5I GGATG 2 cut(s) 210, 412
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 4 cut(s) 210, 247, 462, 551
BstMAI GTCTC 2 cut(s) 295, 565
BstMBI GATC 4 cut(s) 207, 244, 459, 548
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 272
BstV1I GCAGC 1 cut(s) 543
BstZ17I GTATAC 1 cut(s) 593
BsuRI GGCC 4 cut(s) 44, 56, 272, 494
BtgI CCRYGG 2 cut(s) 36, 45
BtgZI GCGATG 1 cut(s) 465
BtsCI GGATG 2 cut(s) 210, 412
BtsIMutI CAGTG 1 cut(s) 593
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 96
CciI TCATGA 1 cut(s) 430
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 271, 337
CseI GACGC 2 cut(s) 39, 230
Csp6I GTAC 2 cut(s) 153, 503
CviAII CATG 4 cut(s) 374, 431, 487, 535
CviQI GTAC 2 cut(s) 153, 503
DdeI CTNAG 4 cut(s) 260, 295, 308, 567
DpnI GATC 4 cut(s) 209, 246, 461, 550
DpnII GATC 4 cut(s) 207, 244, 459, 548
EaeI YGGCCR 2 cut(s) 42, 492
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 1 cut(s) 98
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 197, 207
Eco88I CYCGRG 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 4 cut(s) 377, 434, 490, 538
FaiI YATR 9 cut(s) 375, 380, 432, 468, 488, 507, 525, 536, 593
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FatI CATG 4 cut(s) 373, 430, 486, 534
FblI GTMKAC 2 cut(s) 583, 592
Fnu4HI GCNGC 3 cut(s) 42, 81, 532
FokI GGATG 2 cut(s) 217, 419
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 3 cut(s) 42, 81, 532
FspBI CTAG 1 cut(s) 546
GlaI GCGC 1 cut(s) 164
GluI GCNGC 3 cut(s) 42, 81, 532
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 4 cut(s) 44, 56, 272, 494
HapII CCGG 2 cut(s) 274, 511
HgaI GACGC 2 cut(s) 39, 230
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 4 cut(s) 377, 434, 490, 538
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 2 cut(s) 348, 573
HpaII CCGG 2 cut(s) 274, 511
HphI GGTGA 2 cut(s) 127, 433
Hpy166II GTNNAC 3 cut(s) 220, 584, 593
Hpy188I TCNGA 4 cut(s) 70, 177, 298, 553
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 3 cut(s) 220, 584, 593
Hpy99I CGWCG 4 cut(s) 27, 34, 55, 128
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 3 cut(s) 157, 236, 588
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 4 cut(s) 260, 295, 308, 567
HpySE526I ACGT 1 cut(s) 22
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 4 cut(s) 377, 434, 490, 538
HspAI GCGC 1 cut(s) 163
Kzo9I GATC 4 cut(s) 207, 244, 459, 548
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 2 cut(s) 134, 513
LpnPI CCDG 9 cut(s) 80, 182, 287, 295, 353, 397, 398, 413, 524
Lsp1109I GCAGC 1 cut(s) 543
LweI GCATC 3 cut(s) 150, 270, 414
MaeI CTAG 1 cut(s) 546
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 1 cut(s) 557
MalI GATC 4 cut(s) 209, 246, 461, 550
MboI GATC 4 cut(s) 207, 244, 459, 548
MboII GAAGA 3 cut(s) 200, 203, 289
MhlI GDGCHC 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 2 cut(s) 86, 463
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 1 cut(s) 567
Mox20I TGGCCA 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 347
MscI TGGCCA 1 cut(s) 494
MseI TTAA 1 cut(s) 321
Msp20I TGGCCA 1 cut(s) 494
MspI CCGG 2 cut(s) 274, 511
MspR9I CCNGG 1 cut(s) 274
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 1 cut(s) 274
NdeII GATC 4 cut(s) 207, 244, 459, 548
NlaIII CATG 4 cut(s) 377, 434, 490, 538
NlaIV GGNNCC 2 cut(s) 130, 515
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 557
PagI TCATGA 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 348
PkrI GCNGC 3 cut(s) 43, 82, 533
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
Psp124BI GAGCTC 1 cut(s) 150
PspN4I GGNNCC 2 cut(s) 130, 515
PspPI GGNCC 2 cut(s) 271, 337
RsaI GTAC 2 cut(s) 154, 504
RsaNI GTAC 2 cut(s) 153, 503
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 42, 81, 532
Sau3AI GATC 4 cut(s) 207, 244, 459, 548
Sau96I GGNCC 2 cut(s) 271, 337
SchI GAGTC 1 cut(s) 567
ScrFI CCNGG 1 cut(s) 274
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 3 cut(s) 150, 270, 414
SinI GGWCC 1 cut(s) 337
Sse9I AATT 2 cut(s) 86, 463
SsiI CCGC 3 cut(s) 41, 80, 83
SspMI CTAG 1 cut(s) 546
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 272
TaaI ACNGT 3 cut(s) 157, 236, 588
TaiI ACGT 1 cut(s) 25
TaqI TCGA 3 cut(s) 25, 210, 330
TaqII GACCGA 1 cut(s) 161
TasI AATT 2 cut(s) 86, 463
TatI WGTACW 1 cut(s) 502
TauI GCSGC 2 cut(s) 44, 83
TfiI GAWTC 1 cut(s) 348
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 593
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 1 cut(s) 531
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 2 cut(s) 340, 419
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 1 cut(s) 593
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 1 cut(s) 86
XmiI GTMKAC 2 cut(s) 583, 592
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.