Rroxscaffold_4G00291660

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2 ). Among the enzymes to be stimulated by the calmodulin-Ca(2 ) complex are a number of protein kinases and phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018478
Physical Location & Seq
Reverse (-)
12043452 .. 12044051
600 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00291660.1

Sequence Viewer

Length: 600 bp
ATGGGATCGACGTTCTTCTCTCGAACCATACGTCGAACGACGACCACGGCCTATCAGCTTCCTGACGATCAGATCGCCGAATTCAAGCATGCTTTCAGCCTTTTGGACAAGGACGGCGACGGATCCATCACTACCAAAGAGCTCGGCACTGTGATGCGCTCACTGGGTCAGAAGCCAACTGAAGAAGAGCTCGAGGTGATGATTAAAGAGGTCGACGTAGATGGAAACGGTAGGATTGATTTCCAAGAGTTCGTTAACCTAATGGCCCGGAAGATGCAGGACACTCTCACAGAGGACCAGCTCAAGGAAGCGTTTCGGGTTTTCGATAAGGACCAGAATGGCTTCATTTCTGCCGATGAGCTTCGTCATGTTATGACGAGCCTCGGCGAGACGCTGACGGATGAGCAGGTTAATCAGATGGTTCGTGAAGCTGATATAGACGGTGATGGACAAATCAACTATAAGGAGTTTGTGAAGGTCATGATGGCCAAGAAAGTACATAGCCGGAGCCTCGAGCATTGTAGCAGTGGTTCTAGTAAGGCTCACAAGCCTAAGAAACTCCGTTGTAGAGAGCGTGTACGCCAATGTTTCTACCTCTAG

Protein Analysis

199

Amino Acids

22.83

Weight (kDa)

5.8

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 23 - 93 5.1e-06 Allograft inflammatory factor 1
EF-hand_1 PF00036 27 - 55 3.9e-07 EF hand domain
EF-hand_6 PF13405 27 - 56 6.2e-08 EF-hand domain
EF-hand_7 PF13499 28 - 88 3.4e-18 EF-hand domain pair
EF-hand_9 PF14658 30 - 90 4.5e-07 EF-hand domain
EF-hand_8 PF13833 40 - 90 9.4e-11 EF-hand domain pair
EF-hand_1 PF00036 63 - 90 1.4e-08 EF hand domain
EF-hand_7 PF13499 98 - 162 1.1e-19 EF-hand domain pair
AIF-1 PF21008 99 - 166 9.3e-06 Allograft inflammatory factor 1
EF-hand_6 PF13405 100 - 129 7.2e-10 EF-hand domain
EF-hand_1 PF00036 101 - 128 1.8e-09 EF hand domain
EF-hand_5 PF13202 101 - 125 5.1e-07 EF hand
EF-hand_11 PF08976 102 - 179 4.4e-06 EF-hand domain
EF-hand_9 PF14658 103 - 163 5.8e-06 EF-hand domain
EF-hand_8 PF13833 114 - 161 1.4e-10 EF-hand domain pair
EF-hand_1 PF00036 137 - 163 1.9e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018478)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20701
rosa_chinensis RchiOBHm_Chr1g0364941
rosa_laevigata RLG00000027457
rosa_multiflora Rmu_ssc0000112.1_g000025
rosa_roxburghii Rroxscaffold_4G00291660
rosa_rugosa Rorug01G0318600
rosa_samantha Rh1BG288600 Rh1CG305300 Rh1DG320200
rosa_wichuraiana Rw1G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 397
AccI GTMKAC 1 cut(s) 213
AclWI GGATC 3 cut(s) 13, 117, 130
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 2 cut(s) 498, 579
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 1 cut(s) 85
AluBI AGCT 6 cut(s) 58, 142, 190, 301, 361, 431
AluI AGCT 6 cut(s) 58, 142, 190, 301, 361, 431
Alw21I GWGCWC 2 cut(s) 144, 192
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 3 cut(s) 13, 117, 130
Ama87I CYCGRG 2 cut(s) 191, 512
AoxI GGCC 3 cut(s) 48, 264, 486
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
Asp700I GAANNNNTTC 2 cut(s) 312, 341
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 3 cut(s) 265, 295, 331
AsuC2I CCSGG 1 cut(s) 268
AsuHPI GGTGA 2 cut(s) 208, 455
AvaI CYCGRG 2 cut(s) 191, 512
AvaII GGWCC 2 cut(s) 295, 331
BalI TGGCCA 1 cut(s) 488
BamHI GGATCC 1 cut(s) 122
BanII GRGCYC 2 cut(s) 144, 192
Bbv12I GWGCWC 2 cut(s) 144, 192
BccI CCATC 5 cut(s) 134, 215, 412, 440, 478
BceAI ACGGC 2 cut(s) 63, 130
BcnI CCSGG 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 2 cut(s) 534, 598
BfuAI ACCTGC 1 cut(s) 397
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 2 cut(s) 295, 331
BmeT110I CYCGRG 2 cut(s) 191, 512
BmgT120I GGNCC 3 cut(s) 265, 295, 331
BmiI GGNNCC 2 cut(s) 124, 509
BmrFI CCNGG 1 cut(s) 268
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 2 cut(s) 144, 264
BmuI ACTGGG 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 268
BsaJI CCNNGG 2 cut(s) 45, 382
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 45, 382
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 168
BshFI GGCC 3 cut(s) 50, 266, 488
BsiHKAI GWGCWC 2 cut(s) 144, 192
BsiHKCI CYCGRG 2 cut(s) 191, 512
BsiSI CCGG 2 cut(s) 268, 505
BslI CCNNNNNNNGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 383
BsmBI CGTCTC 1 cut(s) 383
BsnI GGCC 3 cut(s) 50, 266, 488
BsoBI CYCGRG 2 cut(s) 191, 512
Bsp1286I GDGCHC 2 cut(s) 144, 192
Bsp143I GATC 4 cut(s) 5, 67, 72, 122
BspANI GGCC 3 cut(s) 50, 266, 488
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 124, 509
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 3 cut(s) 13, 117, 130
BspQI GCTCTTC 1 cut(s) 180
BsrI ACTGG 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 45, 382
BssMI GATC 4 cut(s) 5, 67, 72, 122
Bst4CI ACNGT 3 cut(s) 151, 230, 443
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 552
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 406
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 4 cut(s) 8, 70, 75, 125
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 4 cut(s) 5, 67, 72, 122
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 122
BstYI RGATCY 1 cut(s) 122
BsuRI GGCC 3 cut(s) 50, 266, 488
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 406
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 3 cut(s) 147, 161, 532
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 1 cut(s) 90
CciI TCATGA 1 cut(s) 480
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 3 cut(s) 265, 295, 331
CseI GACGC 1 cut(s) 400
Csp6I GTAC 2 cut(s) 497, 578
CviAII CATG 3 cut(s) 89, 368, 481
CviQI GTAC 2 cut(s) 497, 578
DdeI CTNAG 1 cut(s) 552
DpnI GATC 4 cut(s) 7, 69, 74, 124
DpnII GATC 4 cut(s) 5, 67, 72, 122
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Ecl136II GAGCTC 2 cut(s) 142, 190
Eco24I GRGCYC 2 cut(s) 144, 192
Eco47I GGWCC 2 cut(s) 295, 331
Eco53kI GAGCTC 2 cut(s) 142, 190
Eco57I CTGAAG 1 cut(s) 201
Eco88I CYCGRG 2 cut(s) 191, 512
EcoICRI GAGCTC 2 cut(s) 142, 190
EcoRI GAATTC 1 cut(s) 80
EcoT38I GRGCYC 2 cut(s) 144, 192
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 3 cut(s) 92, 371, 484
FaiI YATR 8 cut(s) 29, 90, 369, 374, 437, 462, 482, 501
FatI CATG 3 cut(s) 88, 367, 480
FblI GTMKAC 1 cut(s) 213
FokI GGATG 1 cut(s) 413
FriOI GRGCYC 2 cut(s) 144, 192
FspBI CTAG 2 cut(s) 534, 598
GlaI GCGC 1 cut(s) 158
HaeIII GGCC 3 cut(s) 50, 266, 488
HapII CCGG 2 cut(s) 268, 505
HgaI GACGC 1 cut(s) 400
HhaI GCGC 1 cut(s) 159
Hin1II CATG 3 cut(s) 92, 371, 484
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 2 cut(s) 214, 256
HindII GTYRAC 2 cut(s) 214, 256
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 2 cut(s) 268, 505
HphI GGTGA 2 cut(s) 208, 455
Hpy166II GTNNAC 3 cut(s) 214, 256, 578
Hpy188I TCNGA 3 cut(s) 72, 171, 417
Hpy188III TCNNGA 4 cut(s) 21, 62, 425, 481
Hpy8I GTNNAC 3 cut(s) 214, 256, 578
Hpy99I CGWCG 5 cut(s) 13, 36, 43, 122, 218
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 3 cut(s) 151, 230, 443
HpyCH4IV ACGT 3 cut(s) 11, 31, 216
HpyCH4V TGCA 1 cut(s) 277
HpyF3I CTNAG 1 cut(s) 552
HpySE526I ACGT 3 cut(s) 11, 31, 216
Hsp92II CATG 3 cut(s) 92, 371, 484
HspAI GCGC 1 cut(s) 157
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 4 cut(s) 5, 67, 72, 122
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 1 cut(s) 507
LpnPI CCDG 8 cut(s) 75, 149, 263, 281, 311, 347, 392, 518
LweI GCATC 2 cut(s) 144, 264
MaeI CTAG 2 cut(s) 534, 598
MaeII ACGT 3 cut(s) 11, 31, 216
MalI GATC 4 cut(s) 7, 69, 74, 124
MboI GATC 4 cut(s) 5, 67, 72, 122
MboII GAAGA 4 cut(s) 7, 194, 197, 283
MflI RGATCY 1 cut(s) 122
MhlI GDGCHC 2 cut(s) 144, 192
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 1 cut(s) 80
MluNI TGGCCA 1 cut(s) 488
MnlI CCTC 5 cut(s) 187, 202, 286, 392, 521
Mox20I TGGCCA 1 cut(s) 488
MroXI GAANNNNTTC 2 cut(s) 312, 341
MscI TGGCCA 1 cut(s) 488
MseI TTAA 3 cut(s) 204, 255, 411
MslI CAYNNNNRTG 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 488
MspI CCGG 2 cut(s) 268, 505
MspR9I CCNGG 1 cut(s) 268
NciI CCSGG 1 cut(s) 268
NdeII GATC 4 cut(s) 5, 67, 72, 122
NlaIII CATG 3 cut(s) 92, 371, 484
NlaIV GGNNCC 2 cut(s) 124, 509
NmeAIII GCCGAG 2 cut(s) 123, 363
NspI RCATGY 1 cut(s) 92
PaeI GCATGC 1 cut(s) 92
PaeR7I CTCGAG 2 cut(s) 191, 512
PagI TCATGA 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PdmI GAANNNNTTC 2 cut(s) 312, 341
Psp124BI GAGCTC 2 cut(s) 144, 192
PspN4I GGNNCC 2 cut(s) 124, 509
PspPI GGNCC 3 cut(s) 265, 295, 331
PspXI VCTCGAGB 2 cut(s) 191, 512
PsrI GAACNNNNNNTAC 2 cut(s) 514, 546
PsuI RGATCY 1 cut(s) 122
RsaI GTAC 2 cut(s) 498, 579
RsaNI GTAC 2 cut(s) 497, 578
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 2 cut(s) 144, 192
SalI GTCGAC 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 3 cut(s) 204, 255, 411
Sau3AI GATC 4 cut(s) 5, 67, 72, 122
Sau96I GGNCC 3 cut(s) 265, 295, 331
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 2 cut(s) 144, 192
SfaNI GCATC 2 cut(s) 144, 264
Sfr274I CTCGAG 2 cut(s) 191, 512
SinI GGWCC 2 cut(s) 295, 331
SlaI CTCGAG 2 cut(s) 191, 512
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 3 cut(s) 191, 302, 512
SmoI CTYRAG 3 cut(s) 191, 302, 512
SphI GCATGC 1 cut(s) 92
Sse9I AATT 1 cut(s) 80
SspMI CTAG 2 cut(s) 534, 598
SstI GAGCTC 2 cut(s) 144, 192
StyD4I CCNGG 1 cut(s) 266
TaaI ACNGT 3 cut(s) 151, 230, 443
TaiI ACGT 3 cut(s) 14, 34, 219
TaqI TCGA 7 cut(s) 8, 22, 34, 192, 213, 324, 513
TasI AATT 1 cut(s) 80
TatI WGTACW 1 cut(s) 496
Tru1I TTAA 3 cut(s) 204, 255, 411
Tru9I TTAA 3 cut(s) 204, 255, 411
TscAI CASTG 3 cut(s) 154, 168, 532
TspDTI ATGAA 1 cut(s) 334
TspGWI ACGGA 3 cut(s) 135, 413, 551
TspRI CASTG 3 cut(s) 154, 168, 532
VpaK11BI GGWCC 2 cut(s) 295, 331
XapI RAATTY 1 cut(s) 80
XceI RCATGY 1 cut(s) 92
XhoI CTCGAG 2 cut(s) 191, 512
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 2 cut(s) 312, 341
XspI CTAG 2 cut(s) 534, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.