Rw1G029010

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2 ). Among the enzymes to be stimulated by the calmodulin-Ca(2 ) complex are a number of protein kinases and phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018478
Physical Location & Seq
Forward (+)
56685235 .. 56685846
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029010.1

Sequence Viewer

Length: 612 bp
ATGGGATCGACGTTCTTCTCTCCAACCATACGTCGAACGACGACCACGGCCACGGCCACGGCCTATCAGCTTCCTGACGATCAGATCGCCGAATTCAAGCATGCTTTCAGCCTTTTGGACAAGGACGGCGACGGTTCCATCACTACCAAAGAGCTCGGCACTGTGATGCGCTCACTCGGTCAAAAGCCAACTGAAGAAGAGCTCGAGGTGATGATTAAAGAGGTCGACGTAGATGGAAACGGTAGGATTGATTTCCAAGAGTTCGTTAACCTTATGGCCCGGAAGATGCAGGACACTCTCACAGAGGACCAGCTCAAGGAAGCGTTTCGAGTTTTCGATAAGGACCAGAATGGCTTCATTTCTGCCGATGAGCTTCGTCATGTTATGACGAGCCTCGGCGAGACGCTGACGGATGAGCAGGTTAATCAGATGGTTCGTGAAGCTGATATGGACGGTGATGGACAAATCAACTATAAGGAGTTTGTGAAGGTCATGATGGCCAAGAAAGTACATAGCCGGAGCCTCGAGCATTGTAGCAGTGGTTCTAGTAAGGGTCACAAGCCAAAGAAACTCCGTTGTAGAGAGCGTGTACGCCAATGTTTCTACCTCTAG

Protein Analysis

203

Amino Acids

23.12

Weight (kDa)

5.61

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 27 - 97 5.3e-06 Allograft inflammatory factor 1
EF-hand_1 PF00036 31 - 59 4e-07 EF hand domain
EF-hand_6 PF13405 31 - 60 6.4e-08 EF-hand domain
EF-hand_7 PF13499 32 - 92 3.5e-18 EF-hand domain pair
EF-hand_9 PF14658 34 - 94 4.7e-07 EF-hand domain
EF-hand_8 PF13833 44 - 94 9.8e-11 EF-hand domain pair
EF-hand_1 PF00036 67 - 94 1.5e-08 EF hand domain
EF-hand_7 PF13499 102 - 166 6e-19 EF-hand domain pair
AIF-1 PF21008 103 - 170 1e-05 Allograft inflammatory factor 1
EF-hand_6 PF13405 104 - 133 7.4e-10 EF-hand domain
EF-hand_1 PF00036 105 - 132 1.8e-09 EF hand domain
EF-hand_5 PF13202 105 - 129 5.2e-07 EF hand
EF-hand_9 PF14658 107 - 167 4.5e-06 EF-hand domain
EF-hand_8 PF13833 118 - 165 6.1e-11 EF-hand domain pair
EF-hand_1 PF00036 141 - 167 3.3e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018478)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20701
rosa_chinensis RchiOBHm_Chr1g0364941
rosa_laevigata RLG00000027457
rosa_multiflora Rmu_ssc0000112.1_g000025
rosa_roxburghii Rroxscaffold_4G00291660
rosa_rugosa Rorug01G0318600
rosa_samantha Rh1BG288600 Rh1CG305300 Rh1DG320200
rosa_wichuraiana Rw1G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 225
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 3 cut(s) 48, 54, 498
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 2 cut(s) 510, 591
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 1 cut(s) 97
AluBI AGCT 6 cut(s) 70, 154, 202, 313, 373, 443
AluI AGCT 6 cut(s) 70, 154, 202, 313, 373, 443
Alw21I GWGCWC 2 cut(s) 156, 204
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 2 cut(s) 203, 524
AoxI GGCC 5 cut(s) 48, 54, 60, 276, 498
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 2 cut(s) 324, 353
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 3 cut(s) 277, 307, 343
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 220, 467
AvaI CYCGRG 2 cut(s) 203, 524
AvaII GGWCC 2 cut(s) 307, 343
BalI TGGCCA 1 cut(s) 500
BanII GRGCYC 2 cut(s) 156, 204
Bbv12I GWGCWC 2 cut(s) 156, 204
BccI CCATC 5 cut(s) 146, 227, 424, 452, 490
BceAI ACGGC 4 cut(s) 63, 69, 75, 142
BcnI CCSGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 546, 610
BfuAI ACCTGC 1 cut(s) 409
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 2 cut(s) 307, 343
BmeT110I CYCGRG 2 cut(s) 203, 524
BmgT120I GGNCC 3 cut(s) 277, 307, 343
BmiI GGNNCC 2 cut(s) 136, 521
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 2 cut(s) 156, 276
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 280
BsaJI CCNNGG 4 cut(s) 45, 51, 57, 394
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseDI CCNNGG 4 cut(s) 45, 51, 57, 394
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 1 cut(s) 316
BshFI GGCC 5 cut(s) 50, 56, 62, 278, 500
BsiHKAI GWGCWC 2 cut(s) 156, 204
BsiHKCI CYCGRG 2 cut(s) 203, 524
BsiSI CCGG 2 cut(s) 280, 517
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 395
BsmBI CGTCTC 1 cut(s) 395
BsnI GGCC 5 cut(s) 50, 56, 62, 278, 500
BsoBI CYCGRG 2 cut(s) 203, 524
Bsp1286I GDGCHC 2 cut(s) 156, 204
Bsp143I GATC 3 cut(s) 5, 79, 84
BspANI GGCC 5 cut(s) 50, 56, 62, 278, 500
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 2 cut(s) 136, 521
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 192
BssECI CCNNGG 4 cut(s) 45, 51, 57, 394
BssMI GATC 3 cut(s) 5, 79, 84
Bst4CI ACNGT 4 cut(s) 134, 163, 242, 455
Bst6I CTCTTC 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 102
BstDSI CCRYGG 3 cut(s) 45, 51, 57
BstF5I GGATG 1 cut(s) 418
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 3 cut(s) 8, 82, 87
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 3 cut(s) 5, 79, 84
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 278
BsuRI GGCC 5 cut(s) 50, 56, 62, 278, 500
BtgI CCRYGG 3 cut(s) 45, 51, 57
BtsCI GGATG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 159, 544
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 102
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 3 cut(s) 277, 307, 343
CseI GACGC 1 cut(s) 412
Csp6I GTAC 2 cut(s) 509, 590
CviAII CATG 3 cut(s) 101, 380, 493
CviQI GTAC 2 cut(s) 509, 590
DpnI GATC 3 cut(s) 7, 81, 86
DpnII GATC 3 cut(s) 5, 79, 84
EaeI YGGCCR 3 cut(s) 48, 54, 498
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Ecl136II GAGCTC 2 cut(s) 154, 202
Eco24I GRGCYC 2 cut(s) 156, 204
Eco47I GGWCC 2 cut(s) 307, 343
Eco53kI GAGCTC 2 cut(s) 154, 202
Eco57I CTGAAG 1 cut(s) 213
Eco88I CYCGRG 2 cut(s) 203, 524
EcoICRI GAGCTC 2 cut(s) 154, 202
EcoRI GAATTC 1 cut(s) 92
EcoT38I GRGCYC 2 cut(s) 156, 204
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 3 cut(s) 104, 383, 496
FaiI YATR 9 cut(s) 29, 102, 275, 381, 386, 449, 474, 494, 513
FatI CATG 3 cut(s) 100, 379, 492
FblI GTMKAC 1 cut(s) 225
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 2 cut(s) 156, 204
FspBI CTAG 2 cut(s) 546, 610
GlaI GCGC 1 cut(s) 170
HaeIII GGCC 5 cut(s) 50, 56, 62, 278, 500
HapII CCGG 2 cut(s) 280, 517
HgaI GACGC 1 cut(s) 412
HhaI GCGC 1 cut(s) 171
Hin1II CATG 3 cut(s) 104, 383, 496
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 2 cut(s) 226, 268
HindII GTYRAC 2 cut(s) 226, 268
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 2 cut(s) 280, 517
HphI GGTGA 2 cut(s) 220, 467
Hpy166II GTNNAC 3 cut(s) 226, 268, 590
Hpy188I TCNGA 2 cut(s) 84, 429
Hpy188III TCNNGA 3 cut(s) 74, 437, 493
Hpy8I GTNNAC 3 cut(s) 226, 268, 590
Hpy99I CGWCG 5 cut(s) 13, 36, 43, 134, 230
HpyAV CCTTC 1 cut(s) 481
HpyCH4III ACNGT 4 cut(s) 134, 163, 242, 455
HpyCH4IV ACGT 3 cut(s) 11, 31, 228
HpyCH4V TGCA 1 cut(s) 289
HpySE526I ACGT 3 cut(s) 11, 31, 228
Hsp92II CATG 3 cut(s) 104, 383, 496
HspAI GCGC 1 cut(s) 169
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 3 cut(s) 5, 79, 84
LguI GCTCTTC 1 cut(s) 192
LmnI GCTCC 1 cut(s) 519
LpnPI CCDG 7 cut(s) 87, 275, 293, 323, 359, 404, 530
LweI GCATC 2 cut(s) 156, 276
MaeI CTAG 2 cut(s) 546, 610
MaeII ACGT 3 cut(s) 11, 31, 228
MaeIII GTNAC 1 cut(s) 554
MalI GATC 3 cut(s) 7, 81, 86
MboI GATC 3 cut(s) 5, 79, 84
MboII GAAGA 4 cut(s) 7, 206, 209, 295
MhlI GDGCHC 2 cut(s) 156, 204
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 1 cut(s) 92
MluNI TGGCCA 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 5 cut(s) 199, 214, 298, 404, 533
Mox20I TGGCCA 1 cut(s) 500
MroXI GAANNNNTTC 2 cut(s) 324, 353
MscI TGGCCA 1 cut(s) 500
MseI TTAA 3 cut(s) 216, 267, 423
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 500
MspI CCGG 2 cut(s) 280, 517
MspR9I CCNGG 1 cut(s) 280
NciI CCSGG 1 cut(s) 280
NdeII GATC 3 cut(s) 5, 79, 84
NlaIII CATG 3 cut(s) 104, 383, 496
NlaIV GGNNCC 2 cut(s) 136, 521
NmeAIII GCCGAG 2 cut(s) 135, 375
NmuCI GTSAC 1 cut(s) 554
NspI RCATGY 1 cut(s) 104
PaeI GCATGC 1 cut(s) 104
PaeR7I CTCGAG 2 cut(s) 203, 524
PagI TCATGA 1 cut(s) 492
PciSI GCTCTTC 1 cut(s) 192
PdmI GAANNNNTTC 2 cut(s) 324, 353
Psp124BI GAGCTC 2 cut(s) 156, 204
PspN4I GGNNCC 2 cut(s) 136, 521
PspPI GGNCC 3 cut(s) 277, 307, 343
PspXI VCTCGAGB 2 cut(s) 203, 524
PsrI GAACNNNNNNTAC 2 cut(s) 526, 558
RsaI GTAC 2 cut(s) 510, 591
RsaNI GTAC 2 cut(s) 509, 590
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 2 cut(s) 156, 204
SalI GTCGAC 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 3 cut(s) 216, 267, 423
Sau3AI GATC 3 cut(s) 5, 79, 84
Sau96I GGNCC 3 cut(s) 277, 307, 343
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 156, 204
SfaNI GCATC 2 cut(s) 156, 276
Sfr274I CTCGAG 2 cut(s) 203, 524
SinI GGWCC 2 cut(s) 307, 343
SlaI CTCGAG 2 cut(s) 203, 524
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 3 cut(s) 203, 314, 524
SmoI CTYRAG 3 cut(s) 203, 314, 524
SphI GCATGC 1 cut(s) 104
Sse9I AATT 1 cut(s) 92
SspMI CTAG 2 cut(s) 546, 610
SstI GAGCTC 2 cut(s) 156, 204
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 4 cut(s) 134, 163, 242, 455
TaiI ACGT 3 cut(s) 14, 34, 231
TaqI TCGA 7 cut(s) 8, 34, 204, 225, 328, 336, 525
TaqII GACCGA 1 cut(s) 167
TasI AATT 1 cut(s) 92
TatI WGTACW 1 cut(s) 508
Tru1I TTAA 3 cut(s) 216, 267, 423
Tru9I TTAA 3 cut(s) 216, 267, 423
TscAI CASTG 2 cut(s) 166, 544
TseFI GTSAC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 1 cut(s) 346
TspGWI ACGGA 2 cut(s) 425, 563
TspRI CASTG 2 cut(s) 166, 544
VpaK11BI GGWCC 2 cut(s) 307, 343
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 104
XhoI CTCGAG 2 cut(s) 203, 524
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 2 cut(s) 324, 353
XspI CTAG 2 cut(s) 546, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.